• Title/Summary/Keyword: Second cell

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Pulsed Laser System of Ultra-scan Way for Uterus Rehabilitation Treatment (자궁 재활치료를 위한 울트라-스캔 방식의 펄스형 레이저시스템)

  • Kim, Whi-Young
    • The Journal of the Korea Contents Association
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    • v.9 no.6
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    • pp.256-265
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    • 2009
  • Laser output becomes output adjustment from 20 w to 100 w consecutively and time of exposure is available adjustment through water plant in 0.01 seconds. Pulse action can intercept laser beam periodically and supermarket pulse 0.1 $\sim$ between 1ms discharge consist and momentary laser output is increased to 5 $\sim$ 10. Specially, that must remove malignancy cell in womb nine escarps in the case of uterine cancer first of all stability of tube output about pulse by weight very, stable soft switching action area is defined without high frequency transformer leakage inductance ($L_1$) increase and additional series inductor insertion to converter the first main circuit securing zero voltage and marks of switching action in this research specially, because circulation current path of inductor ($L_f$) current is intercepted, converter the first main circuit switching component and spiritual enlightenment damage of high frequency transformer take decreasing greatly and high frequency the second stoppage department ($D_5,\;D_6$) becomes soft switching, and also, switching damage absorption quantity characteristic that can come to life again as subordinate have, and to become tube stabilization design and result that manufacture and experiment, brought result that improve of 10% than existing equipment, and if supplement as systematic late, it becomes thought to get into superior result.

Isolation and Identification of Contaminated Organisms on Dried Persimmon (곶감으로부터의 오염미생물 분리 및 동정)

  • Kang, Bok-Hee;Jo, Mi-Young;Hur, Sang-Sun;Shin, Kee-Sun;Lee, Dong-Sun;Lee, Sang-Han;Lee, Jin-Man
    • Food Science and Preservation
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    • v.19 no.6
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    • pp.939-945
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    • 2012
  • In this study, we isolated microorganisms from dried persimmon in Sangju and obtained 15 strains of microorganisms as the basic research to prevent the quality changes during drying and storage of dried persimmon. Contaminated microorganisms were separated using seven species of medium. Viable cell counts of dried persimmon from Sangju was $5.18{\times}10^2{\sim}1.68{\times}10^7$ CFU/g. Green mold K2-1 accounted for the highest percentage in the contaminated dried persimmons and identified as a major causative microorganism. Light violet and creamy yeasts were the second largest contaminated microorganisms. Green mold K2-1 strain was identified as Penicillium sp. and fungus K-1 and K-3 were identified as Caldosporium sp. and Aspergillus sp.

Effects of Soil Type and Light Condition on Seedling Quality of Rare and Endemic Plants Orostachys iwarenge and Orostachys iwarenge for. magnus (토양 종류와 광 조건이 희귀특산식물 연화바위솔 및 울릉연화바위솔 규격묘 특성에 미치는 영향)

  • Lee, Su Gwang;Lee, Dong Jun;Kim, Hyo Yun;Ku, Ja Jung
    • Korean Journal of Plant Resources
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    • v.27 no.2
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    • pp.174-182
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    • 2014
  • This study was carried out to investigated the effects of soil type and light condition on seedling quality of rare and endemic plants Orostachys iwarenge and Orostachys iwarenge for. magnus. The best seedling quality of O. iwarenge and O. iwarenge for. magnus seedling were shown in Klasmann soil at non-shading or LED (Red+Blue) condition, 50% shading or LED (Red+Blue) condition, respectively. Survival of O. iwarenge seedling was not influenced by the period of experiment but survival of O. iwarenge for. magnus seedling in sandy soil conditions decreased significantly. Results of correlation analysis of seedling quality and survival rate, indicated that O. iwarenge showed a correlation of 0.8 or more at plant height, leaf length and fresh weight. O. iwarenge for. magnus showed a correlation of 0.8 or more at plant height, leaf width and leaf length. Especially, seedling survival of O. iwarenge and O. iwarenge for. magnus showed a correlation of 0.8 or more at first seedling survival and second seedling survival. It was important to manage the early seedling stages of O. iwarenge and O. iwarenge for. magnus. Therefore, seedlings with plant height (over 2.5~4 cm) and root length (over 3~5 cm) were grown vigorously at 35 days after the seedling was transplanted in mid-April in Klasmann soil of 72 plug cell tray at LED (Red+Blue) conditions.

Expression Analysis of Chicken Interleukin-34(IL-34) for Various Pathogenic Stimulations (주요 병원균 자극에 의한 닭의 Interleukin-34 발현 분석 비교)

  • Hong, Yeong Ho
    • Korean Journal of Poultry Science
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    • v.48 no.3
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    • pp.111-122
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    • 2021
  • Recently, interleukin 34 (IL-34) was identified as the second functional ligand for macrophage colony-stimulating factor receptor (M-CSFR). IL-34 functions similarly to M-CSF through its binding to the M-CSFR. There is still insufficient information on IL-34 in chickens, which has until now been reported only through predicted sequences and not through experimental research. Thus, to confirm its expression and to determine its potent biological activity, several chicken lines and cell lines were used. Cloning of recombinant chicken IL-34 and M-CSF genes was performed to investigate their modulatory effects on proinflammatory cytokine expression in vitro. The expression levels of IL-34, M-CSF, and M-CSFR genes were upregulated in broiler chickens with leg dysfunction (cause unknown). However, IL-34 was downregulated in most pathogen-stimulated tissues. M-CSFR expression was enhanced by recombinant IL-34 and M-CSF proteins in vitro. IFN-γ expression was enhanced by recombinant IL-34, but not by M-CSF. However, IL-12 expression was not regulated in any of the treated cells, and IL-1β was decreased in all tissues. These results indicate that IL-34 and M-CSF have roles in both the classical and alternative macrophage activation pathways. Collectively, our findings demonstrate the expression of IL-34 in chickens for pathogenic trials, both in vitro and in vivo. Our results suggest that the IL-34 protein plays a role in both pro- and anti-inflammatory functions in macrophages. Therefore, further research is needed to determine the cytokines or chemokines that can be induced by IL-34 and to further elucidate the functions of IL-34 in the inflammatory pathway.

Weaving the realities with video in multi-media theatre centering on Schaubuhne's Hamlet and Lenea de Sombra's Amarillo (멀티미디어 공연에서 비디오를 활용한 리얼리티 구축하기 - 샤우뷔네의 <햄릿>과 리니아 드 솜브라의 <아마릴로>를 중심으로 -)

  • Choi, Young-Joo
    • Journal of Korean Theatre Studies Association
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    • no.53
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    • pp.167-202
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    • 2014
  • When video composes mise-en-scene during the performance, it reflects the aspect of contemporary image culture, where the individual as creator joins in the image culture through the device of cell phone and computer remediating the former video technology. It also closely related with the contemporary theatre culture in which 1960's and 1970's video art was weaved into the contemporary performance theatre. With these cultural background, theatre practitioners regarded media-friendly mise-en-scene as an alternative facing the cultural landscape the linear representational narrative did not correspond to the present culture. Nonetheless, it can not be ignored that video in the performance theatre is remediating its historical function: to criticize the social reality. to enrich the aesthetic or emotional reality. I focused video in the performance theatre could feature the object with the image by realizing the realtime relay, emphasizing the situation within the frame, and strengthening the reality by alluding the object as a gesutre. So I explored its two historical manuel. First, video recorded the spot, communicated the information, and arose the audience's recognition of the object to its critical function. Second, video in performance theatre could redistribute perceptual way according to the editing method like as close up, slow motion, multiple perspective, montage and collage, and transformation of the image to the aesthetic function. Reminding the historical function of video in contemporary performance theatre, I analyzed two shows, Schaubuhne's Hamlet and Lenea de Sombra's Amarillo which were introduced to Korean audiences during the 2010 Seoul Theatre Olympics. It is known to us that Ostermeir found real social reality as a text and made the play the context. In this, he used video as a vehicle to penetrate the social reality through the hero's perspective. It is also noteworthy that Ostermeir understood Hamlet's dilemma as these days' young generation's propensity. They delayed action while being involved in image culture. Besides his use of video in the piece revitalized the aesthetic function of video by hypermedial perceptual method. Amarillo combined documentary theatre method with installation, physical theatre, and video relay on the spot, and activated aesthetic function with the intermediality, its interacting co-relationship between the media. In this performance theatre, video has recorded and pursued the absent presence of the real people who died or lost in the desert. At the same time it fantasized the emotional aspect of the people at the moment of their death, which would be opaque or non prominent otherwise. As a conclusion, I found the video in contemporary performance theatre visualized the rupture between the media and perform their intermediality. It attempted to disturb the transparent immediacy to invoke the spectator's perception to the theatrical situation, to open its emotional and spiritual aspect, and to remind the realities as with Schaubuhne's Hamlet and Lenea de Sombra's Amarillo.

Removal of mycotoxin ochratoxin A by isolated bacteria (분리세균에 의한 진균독소 ochratoxin A의 제거)

  • Choi, Ho-Yeong;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.55 no.1
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    • pp.33-38
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    • 2019
  • Ochratoxin A (OTA), one of mycotoxins produced mainly by Aspergillus is a common contaminant of stored grains, posing health hazards to human and livestock. The aim of this study is to explore ability of isolated bacteria Bacillus subtilis AF13 and Streptomyces shenzhenensis YR226 to remove OTA. AF13 and YR226 could remove 94.23 and 97.73% of OTA ($100{\mu}g/L$), respectively during 24 h incubation in NB medium. When cultures of two strains were separated into washed cells and cell-free supernatant, the supernatant of both strains removed more than 90% of $100{\mu}g/L$ OTA, and 98.88% of OTA could be also removed by the washed cells of YR226. OTA removal occurred in a few second by the supernatant of both strains, and treatments of autoclaving, proteinase K and chymotrypsin did not affect the OTA removal by the culture supernatants, which indicate that some thermostable and non-proteinaceous substances secreted by these bacteria may be involved in OTA removal in these two bacteria. These results suggest that AF13 and YR226 can be used to remove OTA from OTA-contaminated grains and feeds, and therefore decrease economic damage in agriculture and feed industry.

Fortification of γ-aminobutyric acid and bioactive compounds in whey by co-fermentation using Bacillus subtilis and Lactobacillus plantarum (유청을 이용한 Bacillus subtilis와 Lactobacillus plantarum의 혼합발효를 통한 γ-aminobutyric acid와 생리활성물질 강화)

  • Kim, Geun-young;Lim, Jong-soon;Lee, Sam-pin
    • Korean Journal of Food Science and Technology
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    • v.50 no.6
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    • pp.572-580
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    • 2018
  • Biologically active substances including gamma-aminobutryric acid (GABA) were added into whey by co fermentation using Bacillus subtilis HA and Lactobacillus plantarum EJ2014. The first fermentation using B. subtilis HA with 5% monosodium glutamate (MSG) and 2% glucose enhanced the production of poly-${\gamma}$-glutamic acid (PGA), resulting in higher consistency of $4.09Pas^n$ as well as whey protein peptides. After the second fermentation using L. plantarum EJ2014, the remaining MSG (3.40%) as a precursor was completely converted to 2.21% GABA. Furthermore, the lactose content in whey decreased from 6.73 to 3.68% after co-fermentation, and the tyrosine content increased from 20.47 to 38.24%. Peptides derived of whey proteins were confirmed by SDS-PAGE. Viable cell counts of B. subtilis and L. plantarum were 5.83 log CFU/mL and 9.20 log CFU/mL, respectively. Thus, co-fermentation of whey could produce the novel food ingredient fortified with biologically active compounds including GABA, ${\gamma}$-PGA, peptides, and probiotics.

The dietary effect of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus

  • Hwang, Yo-Sep;Bang, Seok Jin;Kang, Tae Yun;Choi, Jae Hyeok;Jung, Sang Mok;Kang, In Sung;Jeon, Se young;Park, Kwan Ha;Choi, Sanghoon
    • Journal of fish pathology
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    • v.32 no.1
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    • pp.9-20
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    • 2019
  • The study investigated the dietary effects of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus. Tilapia were divided in four groups. The first is a fish group fed a basal diet added with 40% medicinal herbs extract (MHE). The second is a fish group fed a basal diet supplied with $2{\times}10^8CFU/g$ of 2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp, respectively (PB). The third group was fed with a mixture of probiotics (2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp) with the medicinal herbs extract added in basal diet (MHE+PB). The fourth group was fed only a basal diet (C). In a non-specific immune parameters analysis, respiratory burst activity, lysozyme activity, phagocytic activity (PA), alternative complement pathway activity ($ACH_{50}$) and superoxide dismutase (SOD) activity were significantly (p<0.05) increased in the group MHE+PB compared to other groups. Both PB and MHE groups showed a significant (p<0.05) increase in respiratory burst activity, lysozyme activity compared to the control C group, whereas no significant differences were observed in PA, $ACH_{50}$ and SOD activity compared to the control group. In challenging test, fish were administered with Edwardsiella tarda (E. tarda) on 30 days after feeding with each experimental diet and viable E. tarda cell reduction was checked over 21 days post injection. MHE+PB group showed a significantly (p<0.05) reduced E. tarda cells compared to other groups. No significant antibacterial difference (p>0.05) was observed between PB and MHE only treated group. Compared to the control, a significant antibacterial difference (p<0.05) appeared in PB but not in MHE (p>0.05). The results suggest that the probiotics and MHE mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.

A New Dual Connective Network Resource Allocation Scheme Using Two Bargaining Solution (이중 협상 해법을 이용한 새로운 다중 접속 네트워크에서 자원 할당 기법)

  • Chon, Woo Sun;Kim, Sung Wook
    • KIPS Transactions on Computer and Communication Systems
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    • v.10 no.8
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    • pp.215-222
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    • 2021
  • In order to alleviate the limited resource problem and interference problem in cellular networks, the dual connectivity technology has been introduced with the cooperation of small cell base stations. In this paper, we design a new efficient and fair resource allocation scheme for the dual connectivity technology. Based on two different bargaining solutions - Generalizing Tempered Aspiration bargaining solution and Gupta and Livne bargaining solution, we develop a two-stage radio resource allocation method. At the first stage, radio resource is divided into two groups, such as real-time and non-real-time data services, by using the Generalizing Tempered Aspiration bargaining solution. At the second stage, the minimum request processing speeds for users in both groups are guaranteed by using the Gupta and Livne bargaining solution. These two-step approach can allocate the 5G radio resource sequentially while maximizing the network system performance. Finally, the performance evaluation confirms that the proposed scheme can get a better performance than other existing protocols in terms of overall system throughput, fairness, and communication failure rate according to an increase in service requests.

Reaction of Dehydrated Ag$_2$Ca$_5$-A with Cesium. Crystal Structures of Fully Dehydrated Ag$_2$Ca$_5$-A and Ag$_2$Cs$_{10}$-A

  • Kim, Yang;Song, Seong-Hwan;Park, Jong-Yul;Kim, Un-Sik
    • Bulletin of the Korean Chemical Society
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    • v.10 no.3
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    • pp.243-247
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    • 1989
  • Two crystal structures of dehydrated $Ag^+\;and\;Ca^{2+}$ exchanged zeolite A, $Ag_2Ca_$5-A, reacting with 0.01 Torr of Cs vapor at $200^{\circ}C$ for 2 hours and 0.1 Torr of Cs vapor at $250^{\circ}C$ for 48 hours, respectively, have been determined by single crystal X-ray diffraction techniques. Their structures were solved and refined in the cubic space group Pm3m at $21(1)^{\circ}C$. The stoichiometry of first crystal was $Ag_2Ca_5$-A (a = 12.294(1)${\AA}$), indicating that Cs vapor did not react with cations in zeolite A and that of second crystal was $Ag_2Cs_{10}$-A (a = 12.166(1)${\AA}$), indicating that all $Ca^{2+}$ ions were reduced by Cs vapor and replaced by $Cs^+$ ions. Full-matrix least-squares refinements of $Ag_2Ca_5-A\;and\;Ag_2Cs_{10}$-A has converged to the final error indices, $R_1\;=\;0.041\;and\;R_2$ = 0.048 with 227 reflections, and $R_1\;=\;0.117\;an\;n\;fdd\;R_2$ = 0.120 with 167 reflections, respectively, for which I > $3{\sigma}$(I). In the structure of $Ag_2Ca_5$-A, both $Ag^+$ ions and $Ca^{2+}$ ions lie on two crystal symmetrically independent threefold axis sites on the 6-rings; $2\;Ag^+$ ions are recessed 0.33 ${\;AA}$ from the (111) planes of three O(3) oxygens and 5 $Ca^{2+}$ ions lie on the nearly center of each 6-oxygen planes. In the structure of $Ag_2Cs_{10}-A,\;Cs^+$ ions lie on the 5 different crystallographic sites. 3 $Cs^+$ ions lie at the centers of the 8-rings at sites of D4h symmetry. 6 $Cs^+$ ions lie on the threefold axes of unit cell: $4\;Cs^+$ ions are found deep in the large cavity and 2 $Cs^+$ ions are found in the sodalite cavity. One $Cs^+$ ion is found in the large cavity near a 4-ring.