• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1364-1370
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• 2002

The objective of this study was to examine the effect of compensatory growth nutritional regimen on mammary gland growth and lactation. One hundred twenty-two Sprague Dawley female rats (35 days of age) were randomly assigned to either a control or a stair-step compensatory nutrition (SSCN) feeding regimen or an alternating 2-2-3-3-week schedule beginning with 40% energy restriction for 2 weeks followed by re-alimentation (control diet) for 2 weeks. Pup weight gain and milk yield were improved 8% and 8 to 15%, respectively, by the SSCN regimen. The gene expression of $\beta$-casein was 2.3-fold greater in the SSCN group than in the control group during early lactation, but they were greater at all stages of the second lactation. The gene expression of insulin-like growth factor-I was 40% lower in the SSCN group than in the control group during early lactation of the second lactation, but during late lactation it was 80% greater than in the control group. The concentration of serum corticosterone tended to be higher in the SSCN group during the late stage of the first lactation. These results suggest that the stair-step compensatory nutrition regimen improves lactation performance and persistency by modulation of cell differentiation and apoptotic cell death.

• International journal of advanced smart convergence
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• 제8권3호
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• pp.54-60
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• 2019

In this paper, a localization system for indoor objects is proposed. The proposed system consists of Beacons, LED Cells, Main Cell Controller (MCC), and Display. A Beacon is attached at each indoor object, and each LED cell has Beacon Scanner and VLC Transmitter. The Visual Light Communications (VLC) and Power Line Communications (PLC) methods are used to communicate the signals for localization of indoor objects. And the proposed system is designed, and implemented as a prototype. To certify that our propose d system can exactly localize a given indoor object, we take test for the implemented system as a p rototype. Here the location of the given indoor object is known. Test is done in two ways. The first is to check the operation of the detail of the system, and the second is the position recognition of i ndoor object. The second is the test of the implemented system to correctly detect the location of the indoor object with Beacon, while the object with Beacon is moved from location C to A. The experimental result shows that the system is exactly detect the moving locations. The system has the advantages of using previously installed power lines, and it does not need to use LAN lines or optical cables. The proposed system is usefully applied to indoor object localization area.

• Asian Pacific Journal of Cancer Prevention
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• 제16권13호
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• pp.5521-5524
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• 2015

Purpose: This analysis was conducted to evaluate the efficacy and safety of icotinib based regimens in treating patients with non-small cell lung cancer (NSCLC). Methods: Clinical studies evaluating the efficacy and safety of icotinib-based regimens with regard to response and safety for patients with NSCLC were identified using a predefined search strategy. Pooled response rates of treatment were calculated. Results: With icotinib-based regimens, 7 clinical studies which including 5,985 Chinese patients with NSCLC were considered eligible for inclusion. The pooled analysis suggested that, in all patients, the positive reponse rate was 30.1% (1,803/5,985) with icotinib-based regimens. Mild skin itching, rashes and diarrhea were the main side effects. No grade III or IV renal or liver toxicity was observed. No treatment-related death occurred in patients treated with icotinib-based regimens. Conclusions: This evidence based analysis suggests that icotinib based regimens are associated with mild response rate and acceptable toxicity for treating Chinese patients with NSCLC.

• 동의생리병리학회지
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• 제26권1호
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• pp.40-46
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• 2012

Cigarette smoking is known to be associated with various chronic pulmonary and cardiovascular diseases ranging from inflammation to cancer. Not only first-hand smoke but also second-hand smoke is suggested to be a factor of health risk. This study was to investigate whether Platycodi Radix extract administration would alter oxidative stress in lung leading to protection of cigarette smoke-induced lung damage. Sprague-Dawley rats were randomly divided into 3 groups; Intact, Smoke+PR and Smoke+Vehicle. In Smoke+PR and Smoke+Vehicle group, the exposure to cigarette smoke was performed for 15 min/day for 4 weeks in ventilated smoking chamber. The Platycodi Radix extract and saline were orally administrated to Smoke+PR and Smoke+Vehicle group each. The rats of Intact group were just kept in ventilated chamber without cigarette smoke. After the experiment for 4 weeks, the lung tissues were collected for histological observation and immunohistochemistry. In Results, airspace enlargement and goblet cell hyperplasia were observed after 4 weeks' exposure to cigarette smoke. Whereas, the oral administration of Platycodi Radix extract for 4 weeks reduced airspace enlargement and goblet cell hyperplasia. Moreover, the alterations of BAX/Bcl-2 proteins in lung tissues were observed. These results suggest that Platycodi Radix extract ameliorates lung damage in cigarette smoke-exposed rats and has protective effects on second-hand smoke injury.

• 한국환경성돌연변이발암원학회지
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• 제26권1호
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• pp.1-6
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• 2006

To enhance our understanding of toxicity mediated through the pathway by which TCDD stimulates gene expression, we have investigated genes whose expressions are changed after treatment with TCDD and/or MNNG in human Chang liver cell. First, we treated with MNNG and TCDD for two weeks to transform human Chang liver cell. We obtained cell looks like to be transformed and compared the differential gene expression by using cDNA chip (Macrogen) which carrys genes related with signal transduction pathways, oncogenes and tumor suppressor genes, etc. We found that TCDD up- or down-regulated 203 and 111 genes including oncogenes and tumor suppressor genes in human Chang liver cell two fold or more, respectively. Second, we compared the differential gene expression after treatment with TCDD only by using cDNA chip (Superarray) which carrys genes related with cell cycle regulations, and found that TCDD up regulated genes related with cell proliferation as well as cell growth inhibition in human Chang liver cell two fold or more, respectively. These results suggest that toxicity induced by TCDD may reflect sustained alterations in the expression of many genes and that the changes reflect both direct and indirect effects of TCDD.

• 생약학회지
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• 제27권1호
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• pp.32-36
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• 1996

Various culture conditions for cell growth and berberine production in cork tree (Phellodendron amurense Rupr.) were investigated. Callus was induced from cambium tissue of cork tree, and cultured on LS liquid medium supplemented with 0.5 mg/1 2,4-D, 0.1mg/1 BA, and 3% sucrose. Several factors enhancing berberine production and cell growth in cork tree cell cultures were found. Some of them enhanced both cell growth and berberine production, but others resulted in a decoupling of cell growth and berberine production with significant in the specific levels. High level of nitrate (80mM), high level of phosphate (8.98mM), and sucrose (7%), 1.0mg/l IAA were effective in berberine production, whereas low level of nitrate (40mM), and phosphate (2.25mM), and high level of sucrose (7%) in the medium were effective in cell growth. Two stage culture(first stage for cell growth, and second stage for berberine production) increased berberine production almost twice (5.06mg/g dry weight) as much as single stage cultures in berberine production.

• 대한기계학회논문집A
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• 제27권12호
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• pp.2079-2084
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• 2003

In biological cell manipulation, manual thrust or penetration of an injection pipette into an embryo cell is currently performed by a skilled operator, relying on visual feedback information only. Accurately measuring cellular forces is a requirement for minimally invasive cell injections. Moreover, the cellular force sensing is essential in investigating the biophysical properties for cell injury and membrane modeling studies. This paper presents cellular force measurements for the force feedback-based biomanipulation. Cellular force measurement system using piezoelectric polymer sensor is implemented to measure the penetration force of a zebrafish egg cell. First, measurement system setup and calibration are described. Second, the force feedback-based biomanipulation is experimentally carried out. Experimental results show that it successfully supplies real-time cellular force feedback to the operator at tens of uN and thus plays a main role in improving the reliability of biological cell injection tasks.

• 한국시뮬레이션학회논문지
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• 제13권4호
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• pp.1-16
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• 2004

The modelling and simulation of the activation process for the heart system is meaningful to understand special excitatory and conductive system in the heart and to study cardiac functions because the heart activation conducts through this system. This thesis proposes two dimensional cellular automaton(CA) model for the activation process of the myocardium and conducted simulation by means of discrete time and discrete event algorithm. In the model, cells are classified into anatomically similar characteristic parts of the heart and each of cells has a set of cells with preassigned properties. Each cell in this model has state variables to represent the state of the cell and has some state transition rules to change values of state variables executed by state transition function. The state transition rule is simple as follows. First, the myocardium cell at rest stay in passive state. Second, if any one of neighborhood cell in the myocardium cell is active state then the state is change from passive to active state. Third, if cell's state is an active then automatically go to the refractory state after activation phase. Four, if cell's state is refractory then automatically go to the passive state after refractory phase. These state transition is processed repeatedly in all cells through the termination of simulation.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2013년도 추계학술대회
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• pp.943-945
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• 2013

슈반세포의 수초화에 대한 연구는 일차 슈반세포의 분리와 배양의 성공에 의해 가능해지고 있다. 본 연구에서는 슈반세포의 근원으로서 마우스 배아의 척수신경절이 사용되었다. 이 방법에는 세 가지 단계가 있다. 첫 단계는 배아의 척수신경절의 파쇄이고 두 번째 단계는 섬유아세포로부터 슈반세포-뉴런 연합체의 기계적인 분리에 의한 슈반세포의 전구세포의 확장이며 세 번째 단계는 뉴런으로 부터 슈반세포의 분리와 분리된 슈반세포의 확장이다. 우리는 본 과정을 통해 짧은 시간이내에 슈반세포-뉴런 연합체와 슈반세포를 고순도로 분리하였다.

• KSBB Journal
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• 제20권1호
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• pp.1-11
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• 2005

Recently, there has been extremely active in the research of stem cell biology. Stem cells have excellent potential for being the ultimate source of transplantable cells for many different tissues. Researchers hope to use stem cells to repair or replace diseased or damaged organs, leading to new treatments for human disorders that are currently incurable, including diabetes, spinal cord injury and brain diseases. There are primary sources of stem cells like embryonic stem cells and adult stem cells. Stem cells from embryos were known to give rise to every type of cell. However, embryonic stem cells still have a lot of disadvantages. First, transplanted cells sometimes grow into tumors. Second, the human embryonic stem cells that are available for research would be rejected by a patient's immune system. Tissue-matched transplants could be made by either creating a bank of stem cells from more human embryos, or by cloning a patient's DNA into existing stem cells to customize them. However, this is laborious and ethically contentious. These problems could be overcome by using adult stem cells, taken from a patient, that are treated to remove problems and then put back. Nevertheless, some researchers do not convince that adult stem cells could, like embryonic ones, make every tissue type. Human stem cell research holds enormous potential for contributing to our understanding of fundamental human biology. In this review, we discuss the recent progress in stem cell research and the future therapeutic applications.