• Korean journal of applied entomology
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• v.38 no.2
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• pp.145-149
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• 1999

To develop the baculovirus expression vector system (BEVS) adopting p10 gene promoter of Spodoptera exigua nucleopolyhedrovirus (SeNPV), we characterized the p10 gene of SeNPV. The nucleotide sequence of 545 bases including the coding region of p10 gene was determined. Compared with the previously reported SeNPV p10 gene (Zuidema et al., 1993), 4 bases were different in the 5' and 3' flanking region but no difference was found in the coding region. The p10 gene was located within Xho I 1.5 Kb, Sph 1 2.4 Kb and Cla I 4.0 Kb fragments by Southern hybridization analysis. Also, the Sph I 2.4 Kb and the Cla I 4.0 Kb fragments were cloned and their restriction enzyme maps were determined.

• Korean journal of applied entomology
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• v.35 no.3
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• pp.228-231
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• 1996

To produce Spodoptera exigua nuclear polyhedrosis virus (SeNPV) using S. exigua larvae, the efficiency of the in vivo production was analysed by larval instar, inoculum and mortality. The results revealed that the mortality of 4th instar larvae inoculated with 1.OX 10' EIBs per ml was 86.7% and the yields of SeNPV was maximal, demonstrating that 4th instar larvae inoculated with 1 . 0l~o6 PIBs per ml were effective to mass production of SeNPV.

• Korean journal of applied entomology
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• v.43 no.4 s.137
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• pp.329-332
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• 2004

This experiment was conducted to investigate pathogenicity of Spodoptera exigua nucleopolyhedrovirus (SeNPV) with different temperatures for mass production. In laboratory condition, $LC_{50}$ values of SeNPV were $9.797{\times}10^{3}PIBs/mL$ at $20^{\circ}C$ and $3.351{\times}10^{2}PIBs/mL$ at $32^{\circ}C$ in 2nd instar larvae. $LC_{50}$ of the other larval stage were similar to that of 2nd instar. $LT_{50}$ values of SeNPV was 9.0 days in $1.0{\times}10^{3}PIBs/mL$ but 6.9 to 3.5 days in $1.0{\times}10^{4-6}PIBs/mL$ against 3rd instar of Spodoptera exigua. $LT_{50}$ Values of $1.0{\times}10^{4}PIBs/mL$ were 5.7, 5.5 and 4.9 days in 24, 28 and $32^{\circ}C$, respectively. As a results, $LT_{50}$ was shortened with increase of temperatures up to $32^{\circ}C$ and also dependent on viral concentration and larval instars.

• Korean Journal of Organic Agriculture
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• v.23 no.4
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• pp.797-811
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• 2015

This study was carried out to identify the control effect of entomopathogenic microagent against Spodoptera exigua on organic chinese cabbage. In laboratory condition, insecticidal activity of 4 commercial BT pesticides against S. exigua were lower than 10% against second instar S. exigua. The insecticidal activity of entomopathogenic nematode were 33.3%, 83.3% and 100% at the concentration of $1{\times}10^2$, $3{\times}10^2$, $1{\times}10^3nematodes/ml$, respectively. Mixture of BT and nematode showed growth inhibition against S. exigua larvae. S. exigua nucleopolyhedrovirus (SeNPV) of $10^5PIBs/ml$ showed more than 70% insecticial activity. The yield of SeNPV was increased as in higher initial inoculation concentration of NPV, food supply, and growth temperature increased. In greenhouse experiment, the control value of BT and nematode mixture treatment was higher than BT and nematode treatment alone against S. exigua. In treatment of $10^7PIBs/ml$ of SeNPV, S. exigua was controlled completely. In farm condition, mixture of microbial agent and organic agricultural material showed higher control value against lepidopteran pest including S. exigua than BT single treatment.