Effects of Different Temperatures on Pathogenicity of Spodoptera exigua Nucleopolyhedrovirus (SeNPV)

This experiment was conducted to investigate pathogenicity of Spodoptera exigua nucleopolyhedrovirus (SeNPV) with different temperatures for mass production. In laboratory condition, $LC_{50}$ values of SeNPV were $9.797{\times}10^{3}PIBs/mL$ at $20^{\circ}C$ and $3.351{\times}10^{2}PIBs/mL$ at $32^{\circ}C$ in 2nd instar larvae. $LC_{50}$ of the other larval stage were similar to that of 2nd instar. $LT_{50}$ values of SeNPV was 9.0 days in $1.0{\times}10^{3}PIBs/mL$ but 6.9 to 3.5 days in $1.0{\times}10^{4-6}PIBs/mL$ against 3rd instar of Spodoptera exigua. $LT_{50}$ Values of $1.0{\times}10^{4}PIBs/mL$ were 5.7, 5.5 and 4.9 days in 24, 28 and $32^{\circ}C$, respectively. As a results, $LT_{50}$ was shortened with increase of temperatures up to $32^{\circ}C$ and also dependent on viral concentration and larval instars.

온도조건에 따른 파밤나방핵다각체병바이러스(SeNPV)의 병원 활성

파밤나방 핵다각체병바이러스(Spodoptera exigua nucleopolyhedrovirus: SeNPV)의 온도별, 농도별로 병원활성을 조사한 결과는 다음과 같다. SeNPV의 온도에 따른 $LC_{50}$은 2령 $20^{\circ}C$에서는 $9.797{\times}10^{3}PIBs/mL$, $32^{\circ}C$에서는 $3.351{\times}10^{2}PIBs/mL$로 온도가 높아질수록 낮아졌으며, 다른 영기에서도 같은 경향이었다. $20^{\circ}C$에서 SeNPV $1.0{\times}10^{3}PIBs/mL$의 파밤나방 3령 유충에 대한 $LT_{50}$은 9.0일이었으나 SeNPV $1.0{\times}10^{4-6}PIBs/mL$에서는 6.9일-3.5일로 더 짧았다. 24, 28, $32^{\circ}C$의 SeNPV $1.0{\times}10^{4}PIBs/mL$에서는 5.7일, 5.5일, 4.9일의 병원 활성을 보여 온도가 올라갈수록 $LT_{50}$은 짧아졌다. 또한 바이러스 농도가 높고 유충의 영기가 어릴수록 짧아져 $LT_{50}$은 온도 바이러스 농도, 유충영기에 따라 차이가 있었다.