• Animal Systematics, Evolution and Diversity
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• v.26 no.1
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• pp.21-27
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• 2010

Two marine euplotid ciliates, i.e. Euplotes cristatus Kahl, 1932 and E. minuta Yocom, 1930, were collected from the public waterfront of Incheon on the Yellow Sea and from the Songjeong Beach, Busan, in the Strait of Korea, respectively. These two species were verified as unrecorded species in Korea. These species were described based on live observation, protargol impregnation, and silver nitrate impregnation. In addition, the small subunit ribosomal DNA (SSU rDNA) sequences of the two species were compared with previously known sequences of the Euplotes species. Euplotes cristatus has an elongated oval form, size in vivo of $60-84{\times}38-68\;{\mu}m$, 35-50 adoral zone of membranelles (AZM), 10 frontoventral cirri (FVC), 5 transverse cirri (TC), 4-5 caudal cirri (CC), 8 dorsal kineties (DK), 10-16 dorsal cilia of middle DK, and silverline system of single-vannus type. Euplotes minuta has a small ovoid form ($44-53{\times}26-35\;{\mu}m$ in vivo), 31-41 AZM, 10 FVC, 5 TC, 4 CC, 9 DK, 10-12 dorsal cilia of middle DK, and silverline system of single-vannus type.

• Animal Systematics, Evolution and Diversity
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• v.25 no.2
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• pp.167-178
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• 2009

Two poorly known and often confused pleurostomatid ciliates, Litonotus paracygnus Song, 1994 and L. pictus Gruber, 1884, were collected from the coastal waters of Yeonggeumjeong and Bongpo-port, Gangwondo in the East Sea and from the Iwon tide embankment near Ganwol-do, Chungcheongnam-do in the Yellow Sea, Korea. These species were described based on live observations, the protargol-impregnation and morphometrics of the species. Also provided are their diagnoses. The small subunit ribosomal DNA (SSU rDNA) sequences of these species were compared with previously known sequences of related species. The diagnostics of the two Litonotus species are as follows. L. paracygnus: 150-300 $\mu$m long in vivo, strongly contractile neck region, two ellipsoid macronuclei (Ma) and one micronucleus (Mi), 7 left (LSK) and 11-14 right somatic kineties (RSK), 2-4 contractile vacuoles (CV) located on the posterior end, extrusemes (Ex) distributed on the anterior region of the ventral margin only. L. pictus: about 200-600 $\mu$m long in vivo, extremely contractile, beautiful body color with rows of yellow to yellow-brownish cortical pigment granules, 12-21 Ma arranged in moniliform pattern, infrequently vermiform, 7-11 LSK and 18-26 RSK, several CV located on both margins, Ex distributed on the anterior region of the ventral margin only. In this study, this genus was firstly recorded in Korea.

• Korean Journal of Plant Taxonomy
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• v.50 no.2
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• pp.227-231
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• 2020

While conducting a floristic study of Korean hepatics, we discovered two unrecorded species, which were collected from wind holes near the Donggang River, Korea. Mannia fragrans (Balb.) Frye & L. Clark and Mannia androgyna (L.) A. Evans are hereby reported for the first time in Korea. M. androgyna is characterized by pale grayish oil bodies in both the aerenchyma and basal tissue, and saccate spores with a conspicuous proximal disc. M. fragrans is characterized by a gynoecial segment with a whitish apical brush of scales, a commonly aromatic smell, and areolate spores with a conspicuous proximal disc. Two unrecorded species are described and illustrated based on Korean material.

• Korean Journal of Microbiology
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• v.15 no.2
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• pp.77-84
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• 1977

The yeast population on 14 species of seaweeds and in water estimated by cultural mothods over a 5-month period in south-west in Korea, Nine species of yeasts, comprising unidentified one, and one of yeast-like fungi were identified. Fifty phycase were attributed to the difference of cultural method between the present work and earlier repoeters and, to some exent, the higher number of Rhodotorula glutinis which had a prior adaptation to the release of inhibitory polyphenolic materials. Although, to what extent, all division of algae showed a similar variation in yeast population, correlated with month, the rapid decrease of yeast population in August seems to be the cause of exposure of heat irradiation in this month. The cultural estimate of per se fltration without double filter and one of unidentified species are discussed.

• The Korean Journal of Zoology
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• v.35 no.1
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• pp.17-22
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• 1992

The three yolk polypeptides have been isolated and partially characterized. Their molecular weights of YPI, YP2, and YP3 were 48, 000, 47, 000, and 46, 000, respectivelv, as judged by SDS-polyacrvlamide gel electrophoresis. They have different digestion products upon in situ peptide mapping by limited proteolysis. Two-dimensional gel electrophoresis showed that their isoelectric points were heterogeneous from 5.92 to 6.54. And thew showed three different antigen-antibody reactions when each polvpeptides is reacted with antisera made to a mixture of all of three. These data reported here indicate that the yolk proteins are consisted of distinctive polypeptides in Drosophlla sp. (robusta species group).

• Korean Journal of Plant Taxonomy
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• v.51 no.1
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• pp.33-48
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• 2021

We investigated the bryophyte flora of the Gayasan Mountain National Park in Korea by conducting 18 field surveys in from April of 2009 to November of 2016 at various sites on the mountains. During the surveys, we discovered 204 taxa comprising 57 families, 106 genera, 199 species, 2 subspecies, and 3 varieties. Among these, 145 species were reported as new to the flora of Gayasan Mountain. A checklist based on a study of 903 specimens is provided. The most notable species recorded during the surveys were the rare bryophytes Hattoria yakushimensis (Horik.) R. M. Schust., Nipponolejeunea pilifera (Steph.) S. Hatt., Drepanolejeunea angustifolia (Mitt.) Grolle, Lejeuena otiana S. Hatt., Cylindrocolea recurvifolia (Steph.) Inoue and Pogonatum contortum (Menzies ex Brid.) Lesq.

• Korean Journal of Plant Resources
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• v.36 no.3
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• pp.219-224
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• 2023

Melilotus dentatus (Waldst. & Kit.) Desf. is an invasive plant native to Europe, Russia, Uzbekistan, Mongolia and China. It was introduced to North America to create grazing areas for livestock; its growth has since become uncontrollable. Melilotus dentatus is now found in Namyang-eup, Hwaseong-si, Gyeonggi-do, South Korea as an alien plant. This species is morphologically similar to Melilotus suaveolens Ledeb. and Melilotus officinalis (L.) Lam. M. dentatus differs from other species based on its oblong leaflets, distinct teeth, two ovules, surface of the legume that is minutely reticulate-nerved or smooth, and seeds that are dark green to greenish brown. Here, we have provided a detailed description and photographs of the alien species M. dentatus as well as an updated key to five Melilotus taxa studied, which will help prepare a continuous monitoring and management plan.

• Biomedical Science Letters
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• v.8 no.1
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• pp.39-46
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• 2002

Amplified fragment length polymorphism (AFLP) is a recently developed PCR-based high resolution fingerprinting method that is able to generate complex banding patterns which can be used to delineate intraspecific genetic relationships among bacteria. In this study, we have modified and evaluated a PCR-based technique, amplified fragment length polymorphism (AFLP) analysis, for use in fingerprinting strains of Staphylococcus aureus. Single-enzyme amplified fragment length polymorphism (SE-AFLP) analysis was used to perform strain identification of Staphylococus aureus. By careful selection of AFLP primers, it was possible to obtain reproducible and sensitive identification to strain level. AFLP fingerprinting of 5 reference strains of Staphylococcus aureus and 65 strains of Staphylococcus aureus that were isolated from food sources of different area and diverse genomic types of Staphylococcus aureus were recognized. As a result of this study, we found that the AFLP patterns of Staphylococcus aureus isolated from Seoul, Taejeon and Gwang-Ju indicated the close relation with genetic similarity. The main purpose of this study was to find an alternative and reliable fingerprinting method to study the overall genetic diversity, using Staphylococcus aureus species as an example, and observed if the method can be successfully applied to all staphylococcal species.

• Korean Journal of Veterinary Research
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• v.47 no.2
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• pp.147-155
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• 2007

We investigated the safety, immunogenicity and protectivity of mix-crude outer membrane protein (cOMP) vaccine against salmonellosis in animals. The mix-cOMP vaccine was extracted from Salmonella enterica serovar Typhimurium (ST) and Salmonella enterica serovar Enteritidis (SE) and Salmonella enterica serovar Braenderup (SB) isolated from pigs. The mix-cOMP vaccine gave significantly higher antibody response than ST-bacterin and ST-cOMP vaccine in guinea pigs. The survival rates of mix-cOMP vaccinated groups showed significantly higher (100%) than those (0-20%) of unvaccinated control group, challenged with 3 species of Salmonella (ST, SE and SB) in mice. Vaccinated groups in pigs showed reduction of clinical signs, increase of average weight gains, decrease of bacterial recovery rates, compared with unvaccinated groups. Especially, the survival rates (100%) of vaccinated groups in chickens showed higher than that (0%) of unvaccinated group. Based on these results, we suggest that the mix-cOMP Salmonella vaccine developed in this study will be effective for the protection against Salmonellosis caused by the various serotypes Salmonella species in animals.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.08a
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• pp.88-88
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• 2010

We synthesized molybdenum thin films deposited by RF magnetron sputtering and physicochemical analysis was performed. The physical and chemical properties of these films were examined with X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The obtained film at the oxygen ratio of 0% showed crystallinity of cubic Mo(110) phase. After the oxygen ratio increased more than 5% in the sputter gas, the molybdenum films were formed as an amorphous phase. The thickness of the Mo thin film was drastically decreased from 1000 nm to ca 70 nm after introduction of oxygen in the sputter gas confirmed by spectroscopic ellipsometer (SE) and scanning electron spectroscopy (SEM). The calculated band gap of the film deduced from SE data increased from 3.17 to 3.63 eV by addition of oxygen in the sputter gas. The roughness of the Mo film was examined with atomic force microscopy (AFM) and it was dramatically decreased by introducing of oxygen during sputtering. XPS results revealed that the ratio of metallic Mo species in the film decreased by the contents of Mo(VI) species increased at the ratio of oxygen increased in the sputter gas and fully oxidized at low content of oxygen in the sputter gas.