• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.68-68
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• 2018

In this study, we investigated whether S. baicalensis rhizome ethanol extract (SBRE) has antioxidant capacities against oxidative stress induced cellular damage in the HaCaT keratinocytes. Our results revealed that treatment with SBRE prior to hydrogen peroxide ($H_2O_2$) exposure significantly increased the HaCaT cell viability. SBRE also effectively attenuated $H_2O_2$ induced comet tail formation, and inhibited the $H_2O_2$ induced phosphorylation levels of the histone ${\gamma}H2AX$, as well as the number of apoptotic bodies and Annexin V positive cells. In addition, SBRE exhibited scavenging activity against intracellular ROS generation and restored the mitochondria membrane potential loss induced by $H_2O_2$. Moreover, $H_2O_2$ enhanced the cleavage of caspase-3 and degradation of poly (ADP-ribose)-polymerase as well as DNA fragmentation; however, these events were almost totally reversed by pretreatment with SBRE. Furthermore, SBRE increased the levels of HO-1 associated with the induction of Nrf2. Therefore, we believed that SBRE may potentially serve as an agent for the treatment and prevention of neurodegenerative diseases caused by oxidative stress.

• Journal of Life Science
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• 제10권1호
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• pp.51-56
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• 2000

In many cases of human cancer, the appearance of hypersialylated glycan structures is related to a precise stage of the disease ; this may depend on the elebated sialyltransferase activity during carcinogenesis. The aim of this study was to investigate the inhibitory effects of Oriental medicinal herbs on enzymatic activities of two kinds ofsialyltransferase, Gal $\beta$ 1,3GalNAc$\alpha$2,3-sialyltransferase(ST3Gal I) and Gal $\beta$ 1,4GlcNAc $\alpha$2,6-sialyltransterases(ST6Gal I), which are well known as glycosyltransterases associated with cancer. The aqueous extracts of Scutellaria Baicalensis Georgi, Coptidis Rhizoma, Glycyrrhiza urlensis Fisch, Bupleuri Radix and Platycodi Radix were prepared and tested, respectively. At concentration of 100$\mu$g, Glycyrrhiza uralensis Fisch showed the highest inhibitory effects(about 42% and 57%, respectively) on ST3Gal Iand ST6Gal Iactivities. ST3GAl I was inhibited about 23% by Scutellaria baicalensi G댁햐, but not by the other samples, whereas ST6Gal I was inhibited about 20% and 40%,respectively, by Scutellaria baicalensis Georgi and Bupleuri Radix. All inhibitory effects were obtained in a concentration-dependent manner.

• 한국식품영양과학회지
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• 제36권4호
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• pp.470-475
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• 2007

두부에서 분리한 부패 미생물 16균주와 6종류의 병원성 미생물에 대한 황금 에탄을 추출물의 항균활성을 조사한 결과 분리 13균주와 병원성 미생물에 대해서 항균활성을 나타내었으며, 두부 부패 미생물인 KN-4, KN-6, KN-10, KN-20에 대하여 황금 에탄을 추출물 0.05% 이상의 농도에서 성장억제 현상을 나타내었다 선발균주를 동정한 결과, KN-4, KN-6, KN-10과 KN-20 공히 Bacillus sp.로 동정되었다. 황금 에탄을 추출물은 Listeria monocytogenes ATCC 19115, Pseudomonas fluorescens ATCC 21541, Staphylococcus aureus ATCC 29273, Salmonella Typhimurium ATCC 21541, Vibrio parahaemolyticus ATCC 17802, Aeromonas hydrophila KCTC 2358에 대해 항균활성을 나타내었으며, 0.5% 이상 농도에서 성장이 억제되었다 황금에탄올 추출물은 $80^{\circ}C$에서 30min, $100^{\circ}C$에서 30 min, $121^{\circ}C$에서 15 min 열처리에 의해, 공시균주에 대한 항균활성이 다소 감소하는 현상을 나타내었으나 열처리 후에도 두부 부패미생물과 공시 병원성 미생물에 대한 항균활성은 뚜렷하게 유지되었다. 두부 부패 미생물과 병원성 미생물에 대한 황금 에탄을 추출물의 최소저해농도는 각각 0.1%와 0.5%이었다.

• Journal of Acupuncture Research
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• 제19권2호
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• pp.211-220
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• 2002

Objective : This study was undertaken to determine whether Scutellaria baicalensis Georgi extract (SbG) exerts the protective effect against oxidant-induced alterations in organic cation transport in the renal proximal tubule. Methods : Organic cation transport was estimated by examining alterations in tetraethylammonium (TEA) uptake in rabbit renal cortical slices. The slices were treated with 0.2 mM tBHP for 60 min at $37^{\circ}C$. tBl-IP caused an inhibition in TEA uptake by renal cortical slices. Such an effect was accompanied by depressed Na+-K+-ATPase activity and ATP depletion. Result : SbG prevented tBHP-induced inhibition of TEA uptake in a dose-dependent manner at the concentration ranges of 0.05-0.1%. SbG also prevented H2O2-induced reduction in TEA uptake. tBHP-induced inhibition of Na+-K+-ATPase activity and ATP depletion were significantly prevented by 0.05% SbG. Oxidants increased LDH release, which was blocked by SbG. Oxidants caused a significant increase in lipid peroxidation and its effect was prevented by SbG. Conclusion : These results suggest that SbG prevents oxidant-induced alterations in organic cation transport in rabbit renal cortical slices. Such protective effects of SbG may be attributed to inhibition of peroxidation of membrane lipid.

• 대한화장품학회지
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• 제39권2호
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• pp.141-148
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• 2013

자외선 노출 증가로 인한 자외선의 유해성을 방지하기 위해 자외선차단제의 사용이 보편화되었다. 이러한 추세에 맞게 화장품 업계에서는 서로 다른 성분을 혼합하거나 천연물에서 유효성분을 추출하는 연구를 활발하게 진행하고 있다. 본 연구에서는 황금에서 추출한 천연성분인 황금추출물을 함유하는 자외선차단제품의 자외선 차단효과를 확인해보고자 하였다. 총 10명의 지원자를 대상으로 각 제품의 최소홍반량을 측정하였고 자외선차단지수를 구하였다. 그 결과 황금추출물을 함유하는 자외선차단제품과 함유하지 않는 자외선차단제품의 자외선차단지수는 각각 $34.52{\pm}2.13$, $32.67{\pm}1.44$였다. 두 제품 간의 자외선차단지수 차이는 통계적으로 유의하지는 않았지만, 인체를 대상으로 천연물 자외선차단제의 임상적 효과를 평가하였고 제품도포로 인한 이상반응이 없었다는 점에서 의미 있는 시도였다고 생각된다.

• 한국약용작물학회지
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• 제24권2호
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• pp.101-109
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• 2016

Background: The purpose of this study was to evaluate methods to reduce seeding expenses, thereby increasing farm income. This study investigated the effects of rain shelter controlled cultivation and adapted seeding times on the stable seed production of Scutellaria baicalensis Georgi. Methods and Results: Seed germination was conducted under 10 condition compose to control, water washing, cold storage at $4^{\circ}C$ for 15 days, seed sterilization with a benomyl pesticides, hormone treated seed by submerging in 100 ppm $GA_3$ with the cold storage at $20^{\circ}C$ and $25^{\circ}C$, darkness in a covered petridish and illuminated with a 1,500 Lux lamp. There were three cultivation type, open cultivation with non-woven fabric mulching, cultivation with a vinyl covering and rain sheltered in a plastic greenhouse. Sowing dates were April 27, May 18, June 7 and June 28, 2013. Plants were spaced 10 cm apart in rows 30 cm apart. Mixed oil cake fertilizer, $N-P_2O_5-K_2O$ (12-10-10) was applied at $600kg{\cdot}10a^{-1}$. Conclusions: Optimum germination occured in darkness at $25^{\circ}C$ and cold storage after submerging in $GA_3$. The highest seed yields ($4.5kg{\cdot}10a^{-1}$) occurred in the plastic greenhouse for the April 25 sowing. The highest root yield (17%) was found on April 1, under greenhouse conditions.

• 대한본초학회지
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• 제29권6호
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• pp.7-13
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• 2014

Objectives : Anti-obesity drugs that have been developed so far have limited efficacies and considerable adverse effects affecting tolerability and safety. Therefore, most anti-obesity durgs have been withdrawn. We tried to develop anti-obesity agent by combinations from herbs that are used in food ingredients as well as in traditional medicines. Methods : The 80% (v/v) ethanol extracts from Bamboo (Phyllostachys pubescence) leaf (BL) and Scutellaria baicalensis (SB) and their 1:1 combination (BLSB) was evaluated on high fat diet induced obese mice compared to Omega-3 as a positive control. The mice were divided into six groups (n=5), one group fed a normal diet (ND), and the others fed a high fat diets for eight weeks. Two weeks after starting feeding the diets, the high fat diet groups were orally administered vehicle and Omega-3, BL, SB, and BLSB at dosage of 200 mg/kg/day for six weeks. All groups were assayed for body weights, food efficiency ratio, blood biochemistry parameters, and organic tissue weights. Results : BLSB group showed significant reductions in body weight gain and fat weights of liver and epididymal adipose tissue compared to BL or SB alone as well as control. Total-cholesterol and LDL-cholesterol levels significantly decreased, and HDL-cholesterol level increased. In liver tissue, macrovesicular steaotisis was remarkably improved and its fat cell size was also significantly decreased. Conclusions : These results suggested that a combination preparation of bamboo leaf and S. baicalensis has anti-obesity effect and have synergistic effect compared to bamboo leaf or S. baicalesis.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.303.2-304
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• 2002

Wogonin. a flavonoid originated from the root of Scutellaria baicalensis Georgi. is known to exhibit potent anti-inflammatory effects and variable degrees of antioxidant and free radical scavenging effects depending on the experimental systems. In addition. wogonin has been reported to protect neurons from excitotoxic and oxidative injuries in primary cultured rat cortical cells. (omitted)

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2001년도 추계학술대회 및 정기총회
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• pp.80-80
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• 2001

The roots of Scutellaria baicalensis Georgi have been used in the chinese medicine for the treatment of inflammation, fever and headache. Recent studies had shown that baicalin and baicalein showed a detoxifying effect and could inhibit peripheral capillary permeability, and Wogonin was found to inhibit the proliferation of tumor cells. Wogonin has been synthesized as shown in schem. Compound 2 was prepared from 2,6-dimethoxy-1,4-benzoquinone(1) followed by acylation to give compound 3. The base treatment of 3 gave cyclized product which was carried by deprotection of methyl group chemoselectively. Overall step included relatively simple ten steps.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.299-300
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• 2009