Private detectives and investigators offer many services, including executive, corporate, and celebrity protection; pre-employment verification; and individual background profiles. They also investigate computer crimes, personal injury cases, insurance claims and fraud, child custody and protection cases, missing persons cases, and premarital screening. This paper focuses on a private detective or investigator system in most developed U.S.A, but it is not easy to describe that system. Licensing requirements vary in U.S.A. Some States have few requirements, and many other States have stringent regulations. A growing number of States are enacting mandatory training programs for private detectives and investigators. Employment and need of private detectives and investigators is expected to grow faster than the average for all occupations. Thus, it is reasonable that korean government should permit private investigation service find the way to minimize the side effects of private investigation service instead of banning the service totally.
Heo, Seok-Mo;Lee, Sol;Wang, HongTao;Jeong, Jeong Hyeok;Oh, Sang Wook
Journal of Periodontal and Implant Science
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v.46
no.5
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pp.320-328
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2016
Purpose: Human saliva, as a vital part of the immune defense system, contains a number of distinct proteins and peptides. Recently human common salivary protein 1 (CSP1) has been identified as an abundant salivary protein and may play a role in promoting the binding of cariogenic bacteria to salivary pellicles. However, nothing else is known regarding the role of CSP1 in periodontology. The aim of this study was to quantify and compare CSP1 levels between healthy subjects and periodontal patients. Methods: This controlled clinical study was conducted in periodontally healthy individuals and patients with chronic periodontitis Chonbuk National University Hospital, with Institutional Review Board approval. Whole saliva samples were collected from 36 healthy subjects and 33 chronic periodontitis patients and analyzed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immune blotting were conducted to ensure that anti-CSP1 monoclonal antibody (mAb) binds to CSP1 in human saliva. A sandwich enzyme-linked immunosorbent assay (ELISA) system was house-fabricated using mAb-hCSP1#14 and mAb-hCSP1#4 as a capture and a detector mAb, respectively. The CSP1 concentrations in saliva from 36 healthy subjects and 33 periodontal patients were quantified using the CSP1 sandwich ELISA system, and the results were analyzed using the Student's t-test. Results: Immunoblot analysis using mAb-hCSP1 as a probe confirmed that CSP1 in human saliva existed as a single band with a molecular weight of approximately 27-kDa. The quantification of CSP1 concentrations by CSP1 ELISA showed that the median values (25th to 75th percentiles) of periodontal patients and healthy subjects were 9,474 ng/mL (range, 8,434.10,139 ng/mL) and 8,598 ng/mL (range, 7,421.9,877 ng/mL), respectively. The Student's t-test indicated the presence of a statistically significant difference between the 2 groups (P=0.024). Conclusions: The presence of a significant difference in CSP1 levels between healthy subjects and periodontal patients suggests that CSP1 may be a potential biomarker for the detection or screening of periodontitis patients.
Nighttime sea fog detection from satellite is very hard due to limitation in using visible channels. Currently, most widely used method for the detection is the Dual Channel Difference (DCD) method based on Brightness Temperature Difference between 3.7 and 11 ${\mu}m$ channel (BTD). However, this method have difficulty in distinguishing between fog and low cloud, and sometimes misjudges middle/high cloud as well as clear scene as fog. Using CALIPSO Lidar Profile measurements, we have analyzed the intrinsic problems in detecting nighttime sea fog from various satellite remote sensing algorithms and suggested the direction for the improvement of the algorithm. From the comparison with CALIPSO measurements for May-July in 2011, the DCD method excessively overestimates foggy pixels (2542 pixels). Among them, only 524 pixel are real foggy pixels, but 331 pixels and 1687 pixels are clear and other type of clouds, respectively. The 514 of real foggy pixels accounts for 70% of 749 foggy pixels identified by CALIPSO. Our proposed new algorithm detects foggy pixels by comparing the difference between cloud top temperature and underneath sea surface temperature from assimilated data along with the DCD method. We have used two types of cloud top temperature, which obtained from 11 ${\mu}m$ brightness temperature (B_S1) and operational COMS algorithm (B_S2). The detected foggy 1794 pixels from B_S1 and 1490 pixel from B_S2 are significantly reduced the overestimation detected by the DCD method. However, 477 and 446 pixels have been found to be real foggy pixels, 329 and 264 pixels be clear, and 989 and 780 pixels be other type of clouds, detected by B_S1 and B_S2 respectively. The analysis of the operational COMS fog detection algorithm reveals that the cloud screening process was strictly enforced, which resulted in underestimation of foggy pixel. The 538 of total detected foggy pixels obtain only 187 of real foggy pixels, but 61 of clear pixels and 290 of other type clouds. Our analysis suggests that there is no winner for nighttime sea fog detection algorithms, but loser because real foggy pixels are less than 30% among the foggy pixels declared by all algorithms. This overwhelming evidence reveals that current nighttime sea fog algorithms have provided a lot of misjudged information, which are mostly originated from difficulty in distinguishing between clear and cloudy scene as well as fog and other type clouds. Therefore, in-depth researches are urgently required to reduce the enormous error in nighttime sea fog detection from satellite.
Kim, Gi Yong;Kang, Sung-Min;Jang, Sung-Chan;Huh, Yun Suk;Roh, Changhyun
Korean Journal of Environmental Biology
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v.34
no.2
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pp.107-115
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2016
Currently, there are 442 operating nuclear power plants in the world, and 62 more are under construction. According to this reasoning, the treatment of radioactive waste is important to prevent the environmental ecosystem including humans, animals, and plants. Especially, a leakage of radioactive waste causes not only regional problem but also serious global one. In this study, we demonstrate the effect of radioisotopes (e.g., cesium, strontium, and cobalt) on a 3D culture cell. To develop the 3D cell culture system, we used a 96-well-culture plate with biocompatible agarose hydrogel. Using this method, we can perform the 3D cell culture system with three different cell lines such as HeLa, HepG2, and COS-7. In addition, we conducted a cell viability test in the presence of radioisotopes. Interestingly, the 3D morphological cells showed 42% higher cell viability than those on the 2D against cesium. This result indicates that the 3D platform provides cells morphological and physiological characteristic similar to in vivo grown tissues. Moreover, it overcomes the limitation of conventional cell culture system that can't reflect in vivo systems. Finally, we believe that the proposed approach can be applied a new strategy for simple high-throughput screening and accurate evaluation of metal toxicity assay.
This study examines the case of living lab applied in the R&D initiatives for solving societal problems and challenges. It discusses how to use the living lab in national R&D projects. The analyzed cases are 'Develop portable fundus camera for eye disease screening test to resolve health inequalities' and 'Auto-sensing integrated system development in rural pedestrian crosswalk'. As a result of the analysis, both cases were designed as a user participatory R&D structure by utilizing living lab. In other words, living lab has operated as a system that evolves technology-products-services into an infrastructure. It can realize final demand specification, product, service improvement and demonstration through continuous interaction of end users. As a result of the case analysis, the following policy tasks can be derived. First, living lab is a new concept and it is in the early stage of implementation in Korea. Therefore, it is necessary to monitor and evaluate living lab experiments and build suitable models for Korean society by sharing cases and achievements. Second, the strategic niche management are necessary for the introduction of living lab. Third, living lab can be used as a tool to transform the existing technology acquisition centered innovation policy to the policy for customer needs and problem solving. Fourth, there is a need for flexibility and adaptability in strategy and system to correct errors that appear in the living lab processes.
Journal of the Institute of Electronics Engineers of Korea SC
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v.46
no.2
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pp.15-21
/
2009
Hearing loss is one of the most common birth defects among infants. Most of hearing-impaired children are not diagnosed until 1 to 3 years of age - which is too late for the critical period (6 month) for normal speech and language development. If a hearing impairment is identified and treated in its early stage, child's speech and language skills could be comparable to his or her normal-hearing peers. For these reasons, hearing screening at birth and throughout childhood is extremely important. ABR (Auditory brain-stem response) is nowadays one of the most reliable diagnostic tools in the early detection of hearing impairment. In this study, we have developed the system that automatically detects if there is hearing impairment or not for infants or children. For future studies, it will be developed as a portable system to be able to take a measurement not only in sound proof room but also in nursery for neonates.
Kim, J.D.;Kwon, C.H.;Shin, C.N.;Kim, C.H.;Kim, D.A.
Asian-Australasian Journal of Animal Sciences
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v.18
no.7
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pp.997-1002
/
2005
The objective of this experiment was to determine agronomic characteristics, and forge yield and quality responses of rye variety to location and year for a double-cropping system in South Korea. Five forage rye varieties ('Koolgrazer', 'Jlee select', '9909', 'Homill22', 'Danko') were evaluated for forage quality and yields during the two growing years (the First year; 2001-2002, and the Second year; 2002-2003) at two locations (the central and southern zone). The heading date of rye in the central zone (Cheonan) was 15 April, and that of the southern zone (Daegu) was 10 April. The heading dates of 'Koolgrazer', 'Jlee select' and '9909' were earlier than those of the others. Although the five varieties had resistance to foliar diseases and insects, these had less lodging resistance. Dry matter (DM) content, DM and total digestible nutrients (TDN) yields had significant differences among rye varieties. DM and TDN yields of the rye varieties at the second year were higher than the first year in the central zone. In the southern zone, DM and TDN yields at the first year were lower than the second year. 'Jlee select' and '9909' had higher DM and TDN yields than the other varieties in both location and year except the first year of the central zone (p<0.001). The crude protein (CP) contents of rye grown in Cheonan were higher than Daegu. The CP content of 'Danko' variety was highest among tested varieties. The acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents were greater for the varieties grown in Cheonan and in the second year. The ADF and NDF contents of 'Danko' were lowest, while 'Danko' in TDN and relative feed value (RFV) was highest among rye varieties. Our study showed that there are differences in forage production and quality from location to location, year to year and variety to variety. Therefore, forage quality and forage production are an important factor for screening forage rye varieties. Early maturing varieties should be recommended as high-yielding forage in a double-cropping system because their production of forage was greatest early in the season.
In this study, a Korean native cattle strain (Hanwoo) evidencing high performance in terms of both meat quality and quantity was employed in the generation of 150,000 BAC clones with an average insert size of 140 kb, and corresponding to about a 6X coverage of bovine chromosomal DNA. The BAC clones were pooled in a mini-scale via three rounds of a pooling protocol, and the efficiency of this pooling protocol was evaluated by testing the accuracy of accessibility to the positive clones, via a PCR-based screening method. Two sets of primers designed from each of two known genes were tested, and each yielded 2 or 3 positive clones for each gene, thereby indicating that the BAC library pooling system was appropriate with regard to the accession of the target BAC clones. Analyses of $3.3{\times}10^6$ base pairs obtained from the 7,090 BAC end sequence (BES) showed that 34.88% of the DNA sequence harbored the repetition sequence. Analysis of the 7,090 BES to the $1^{st}$ and $2^{nd}$ generation radiation hybrid map of the cattle genome, using the COMPASS program designed for the construction of a cattle-human comparative mapping, resulted in the localization of a total of 1,374 clones proximal to 339 $1^{st}$ generation markers, and 1,721 clones proximal to 664 $2^{nd}$ generation markers. Collectively, the BAC library and pooling system of the BAC clones from the Korean cattle, coupled with the chromosome-localized BAC clones, will provide us with novel tools for the excavation of desired clones for genome mapping and sequencing, and will also furnish us with additional information regarding breed differences in cattle.
Screenings were performed on edible plants to examine their complement-system activating ability (anti-complementary activity) by hemolytic complement assay $(TCH_{50})$. Among 38 kinds of plant extracts, 5 kinds showed relatively strong anti-complementary activity which decreased $TCH_{50}$ more than 60% comparison with control and the order of activity was Zingiber officinale>Colocasia antiquorum>Capsella bursapastoris>Ginkgo biloba>Alium monanthum in $1000{\mu}g/ml$. The anti-complementary activity of ZR-1 prepared from the root of Zingiber officinale which was showed the most potent activity, did not change by pronase treatment, but decreased greatly by periodate oxidation. These results indicate that not protein moiety but carbohydrate moiety in ZR-1 fraction may also contribute to the anti-complementary activity. Also, the anti-complementary activity of ZR-1 was reduced partially in the absence of the $Ca^{2+}$ ion. When crossed immunoelectrophoresis using anti-human C3 serum was carried out after incubation of normal human serum with the ZR-1 in $Ca^{2+}$ free condition, a cleavage of C3 precipitin line was observed. Furthermore this polysaccharide fraction considerably inhibited $ACH_{50}$. These results also indicate that the mode of complement activation by polysaccharide from Zingiber officinale is via not only the classical pathway but also the alternative pathway.
This study is focusing on activating and developing the right model of win-win partnership innovative activity for the purpose of win-win relationship among companies. These are the following studies that are conducted for that objective. First, This study systematically analyzes the best cases of the win-win partnership focusing on the proceedings of the companies participating in the field of win-win Partnership at the national quality-circle contest for the last three years(2014~2016). This study reveals the underlying problems of win-win partnership innovative activity and draws the conclusion in the way of improvement about the win-win partnership innovative activity. Second, This study suggests the efficient method of the function and improvement of a system for activating win-win partnership innovative activity by analyzing screening criterion and selecting excellent cases in the field of win-win Partnership at the national quality-circle contest in 2017. Third, This study suggests the method of strengthening the system of sharing result for invigorating and motivating the win-win partnership innovative activity. It is expected that this methodology for reinforcing the system of Win-win Partnership among companies will help to create the environment where a sound and new corporate culture can be settled. And It is also expected that this study can contribute to attaining the goal of growing together through enhancing the competitiveness, productivity and quality among Win-win Cooperation companies.
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