• 제목/요약/키워드: ScpB

검색결과 27건 처리시간 0.019초

Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria

  • Park, Hye-Jee;Han, Sang-Wook
    • The Plant Pathology Journal
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    • 제33권6호
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    • pp.602-607
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    • 2017
  • Segregation and condensation protein B (ScpB) is essential for replication and segregation in living organisms. Here, we reported the functions of ScpBXv (ScpB-like protein in Xanthomonas campestris pv. vesicatoria) using phenotypic and proteomic analyses. Growth of $Xcv{\Delta}scpBXv$ (ScpBXv knockout mutant) was reduced under both slow and fast growth conditions in rich medium, but comparable to this of the wild-type in plant-mimic conditions. Interestingly, the mutant was significantly less virulent than the wild-type in tomato, indicating that ScpBXv is involved in virulence. To investigate ScpBXv-associated mechanisms, comparative proteomic analyses were carried out and the abundance of 187 proteins was altered. Among them, diverse transcriptional regulators involved in biofilm formation and virulence were abundant in the wild-type. We further showed that biofilm formation of $Xcv{\Delta}scpBXv$ was reduced. This study provides new insights into the functions of ScpBXv in bacterial replication and biofilm formation, which may contribute to the virulence of Xcv.

Effect of the Addition of Schisandra chinensis Powder on the Physico-chemical Characteristics of Sausage

  • Jin, S.K.;Park, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제26권12호
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    • pp.1753-1761
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    • 2013
  • The individual and interactive effects of Schisandra chinensis powder (SCP) and sodium nitrite additions on color, pH, water holding capacity, residual nitrite, 2-thiobarbituric acid reactive substances (TBARS), volatile basic nitrogen, texture properties, fatty acids, amino acids and sensory evaluation of cooked pork sausages were investigated after 20 d of storage at $4^{\circ}C$. The powders (0, 0.5 and 1.0%) were added to sausages either alone or in combination with nitrite (0 and 100 ppm). SCP added-sausages showed lower $L^*$ (lightness) and W (whiteness) values, and higher $b^*$ (yellowness) values than sausage containing no nitrite, and exhibited the highest $a^*$ values at a 0.5% addition (p<0.05). Residual nitrite and TBARS values were found to be significantly reduced as the addition levels of SCP increased (p<0.05). As the addition of SCP increased, the sausage showed gradually decreased brittleness, cohesiveness, springiness, gumminess and chewiness, while adhesiveness increased. Polyunsaturated fatty acid, n-6 and n-6/n-3 fatty acid ratio concentrations were significantly higher in sausages containing SCP (p<0.05). The addition of SCP to sausage significantly (p<0.05) increased the ammonia content (by 0.5% SCP) and aromatic amino acid concentrations (by 1.0% SCP) (p<0.05). Inclusion of SCP in sausage meat resulted in a significant deterioration in quality characteristics of flavor, springiness, juiciness and overall acceptability (p<0.05). As expected, the observed changes in $a^*$, W, pH, shear force, texture property, TBARS, fatty acid, amino acid and sensory score of sausages, depended on the rate of addition of nitrite (p<0.05). These results suggest that SCP addition is not an effective way of improving the sensory evaluation of sausages, but may beneficially affect TBARS, nitrite scavenging activity, fatty acid and amino acid content in pork sausages.

집중체중 이동훈련이 뇌성마비 아동의 체간조절 능력에 미치는 영향: 사례연구 (The Effect of Intensive Weight Shift Training on Seated Postural Balance in Children With Spastic Cerebral Palsy: A Case Study)

  • 유현남;한진태
    • 대한감각통합치료학회지
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    • 제17권1호
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    • pp.9-18
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    • 2019
  • 목적 : 본 연구의 목적은 경직성 뇌성마비아동의 앉은 자세균형에 대한 집중체중 이동훈련의 효과를 알아보고자 하였다. 연구방법 : 경직성 사지마비로 진단 받은 11세 여자를 대상으로 ABA 실험설계를 이용하여 8주간 실험을 진행하였다. 기초선(A1)에서 일반 물리치료를 수행하였고, 중재 중(B1, B2)에서 일반 물리치료와 집중체중 이동훈련을 실시하였으며, 중재 후(A'1, A'2)에서 다시 일반 물리치료를 실시하였다. 체간조절능력은 한국형 체간조절 측정척도(TCMS-K)와 균형측정시스템(Biorescue, RM Ingenierie, France)를 사용하여 측정하였다. 연구결과 : 한국형 체간조절 측정척도의 모든 항목이 집중체중 이동훈련 기간 중에 증가하였다. 또한 안정성한계 면적이 집중체중 이동훈련 기간 중에 증가하였고 왼쪽/오른쪽 그리고 앞/뒤 안정성한계 면적이 집중체중 이동훈련 기간 중에 대칭적이었다. 결론 : 집중체중 이동훈련이 경직성 뇌성마비 아동의 체간조절 능력을 개선하는데 긍정적인 효과가 있었다. 본 연구는 집중체중 이동훈련이 경직성 뇌성마비 아동의 독립적 앉기, 자세조절, 일상생활을 위한 균형능력 개선에 도움이 될 것으로 사료된다.

모래다짐말뚝의 원심모델링 (Centrifugal Modeling of Sand Compaction Pile)

  • 유남재;정길수;김상진;채승호
    • 산업기술연구
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    • 제21권B호
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    • pp.187-193
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    • 2001
  • This paper is results of extensive centrifuge model experiments about design factors influencing the bearing capacity and the settlement behaviors of SCP (Sand Compaction Pile). Centrifuge model tests were carried out changing design factors for SCP method such as replacement area ratio (as= 20, 40, 70%), improvement ratio to footing width (W/B = 1, 2, 3), and amount of fines in sand pile (#200 = 5, 10, 15). Therefore, the effects of these design factors on the bearing capacity and the settlement behavior of SCP were investigated and changes of stress concentratio rato due to such an design factors were also investigated. Centrifuge model testing technique for preparing and installing centrifuge model of sand compaction pile, using freezing them, was also developed. As results of centrifuge model tests, more fines in sand compaction pile increases the bearing capacity of SCP. Optimum improvement ratio to footing width was found to be 2. Values of stress concentration ratio was in the ranges of 1.5 - 3.5. The depth of bulging in sand piles was found in the range of 2.0 - 2.5 times of pile diameter.

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Sheet Pile 설치에 따른 SCP개량지반의 거동 (Behavior of SCP Improved Ground with Installation of Sheet Pile)

  • 유남재;박병수;정길수
    • 산업기술연구
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    • 제22권B호
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    • pp.211-218
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    • 2002
  • The paper is to show the behavior of composit ground which is installed with sheet pile in soft soil improved by sand compaction pile. The results of load-settlement relationship, earth pressure, stress concentration characteristics, and final water content were obtained by centrifuge model test. Two cases of tests, installation of sheet pile on the corner and both side of the loading plate for the improved SCP ground which was designed twice of the footing width, were performed for the tests under the vertical and horizontal loading and both side of corner. Finite element program(CRISP) for sand compaction pile using elasto-plastic model and numerical analysis for soft soil using modified cam-clay constitutive equation were compared and analized with the results of model tests. The result of analysis show the increased bearing capacity of soil after, SCP and sheet pile was installed.

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용접 판재의 U 드로오 벤딩에서 스프링백 예측을 위한 이론적 단순화 (A Simplified Approach for Predicting Springback in U-Draw Bending of Sheet Metals)

  • 장성호;서대교
    • 소성∙가공
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    • 제13권8호
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    • pp.678-688
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    • 2004
  • The U-draw bending operation is known as a representative test method for springback evaluation of sheet metals since the sheet in U-draw bending operation undergoes stretching, bending and unbending deformations occurred at the stamping process. In this study, a simplified approach was proposed for predicting springback and side-wall curls of tailor-welded blank in U-draw bending operations, using moment-curvature relationships derived for sheets undergoing stretching, bending and unbending deformation. Two different welded strips were adopted to compare the effects of weld-line locations on the springback. One (type A) was welded along the centerline of the strip-width and the other (type B) was welded along the centerline of the strip-length. To investigate the effect of different thickness combination on the springback, the tailor-welded strips were joined by the laser welding process and consisted of three types of thickness combinations of sheets, SCP1 0.8t * SCP1 1.2t, SCP1 0.8t * SCP1 1.6t and SCP1 0.8t * TRIP 1.0t. Some calculated results by the simplified formula were compared with experimental results.

SCP 보강 점성토 지반의 지지력 및 응력분담특성 (Characteristics of Bearing Capacity and Stress Concentration of Clay Ground Improved with Sand Compaction Piles)

  • 유남재;박병수;정길수;고경환;김지성
    • 한국지반공학회논문집
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    • 제21권1호
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    • pp.81-91
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    • 2005
  • 본 논문은 SCP로 보강된 점성토 지반의 파괴메카니즘 및 응력분담비, 지지력등에 미치는 설계변수에 대한 실험적 수치적 연구 결과이다. SCP로 보강된 점성토 지반의 거동을 알아보기 위하여 SCP의 치환율을 20, 40, $70\%$로 변화시키고, 비소성 세립분 함유량을 5, 10, $15\%$로 변화시켰으며 하중재하폭에 대한 지반개량폭의 비를 1, 2, 3으로 변화시키는 광범위한 원심모형실험을 실시하였다. 한편, 원심모형실험 결과를 모사하기 위하여 상용 유한요소 프로그램인 CRISP을 이용하였으며, 수치해석시 모래다짐말뚝은 탄소성모델로 점토지반은 한계상태이론에 기초한 수정 Cam-clay 모델을 사용하였다.

Nd:YAG 레이저빔을 이용한 SCP 강판과 STS304강판 용접시 오프셋(off-set) 위치 결정에 관한 연구 (A study of the determination of off-set position for Nd:YAC laser welding between SCP steel sheet and STS304 sheet)

  • 윤부선;김도훈;박기영;이경돈
    • 한국레이저가공학회지
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    • 제7권2호
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    • pp.1-10
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    • 2004
  • This work was attempted to join SCP sheet and STS304 sheet by using Nd:YAC laser beam. SCP sheet has good formability and low cost, while STS304 has excellent corrosion resistance and mechanical properties in high temp. In this experiment, butt joint type was used to develop the tailored blank welding for dissimilar steel. Sheets which have different thermal properties. Computer simulation was conducted to obtain the off-set position for efficient welding by considering laser power, scanning speed, focal length and basic properties. The result showed that the optimum thermal distribution was obtained when the laser beam was irradiated at $0.05{\sim}0.1$ mm off-set toward the SCP sheet side. The experiment was conducted based on the result of computer simulation to show the same optimum conditions. Optimum conditions were 3KW in laser beam power, 6m/min in scanning speed, -0.5mm in focal position, 0.1mm off-set toward SCP. Microhardness test, tensile test, bulge test, optical microscopy, EDS, and XRD were performed to observe the microstructure around fusion zone and to evaluate the mechanical properties of optimum conditions, The weld zone had high microhardness values by the formation of the martensitic structure. Tensile test measured the strength of welded region by vertical to strain direction and the elongation of welded region by parallel to strain direction. Bulge test showed $52\%$ formability of the original materials. Bead shape, grain size, and martensitic structure were observed by the optical microscopy in the weld zone. Detailed results of EDS, XRD confirmed that the welded region was connected of martensitic structure.

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모래다짐말뚝으로 개량된 연약지반의 응력분담특성 (Stress Concentration Characteristics of Soft Ground Treated by Sand Compaction Pile)

  • 유남재;박병수;정길수;김상진
    • 산업기술연구
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    • 제22권A호
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    • pp.145-151
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    • 2002
  • This paper is results of extensive centrifuge model experiments about design factors influencing the bearing capacity and the settlement behaviors of SCP (Sand Compaction Pile). Centrifuge model tests were carried out changing design factors for SCP method such as replacement area ratio (as= 20, 40, 70%), Improvement ratio to footing width (W/B = 1, 2, 3), and amount of fines m sand pile (#200 = 5, 10, 15). Therefore, the effects of these design factors on the bearing capacity and the settlement behavior of SCP were investigated and changes of stress concentratio rato due to such an design factors were also investigated. Centrifuge model testing technique for preparing and installing centrifuge model of sand compaction pile, using freezing them, was also developed. As results of centrifuge model tests, more fines in sand compaction pile increases the bearing capacity of SCP. Optimum improvement ratio to footing width was found to be 2. Values of stress concentration ratio was in the ranges of 1.5 - 3.5. The depth of bulging in sand plies was found in the range of 2.0 - 2.5 times of pile diameter.

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초음파분무 연소법에 의한 나노결정 ZnO 초미분체 제조 (Preparation of Nanocrystalline ZnO Ultrafine Powder Using Ultrasonic Spraying Combustion Method)

  • 김광수;황두선;구숙경;이강;전치중;이은구;김선재
    • 한국재료학회지
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    • 제12권10호
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    • pp.784-790
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    • 2002
  • For mass product of nanocrystalline ZnO ultrafine powders, self-sustaining combustion process(SCP) and ultrasonic spray combustion method(USCM) were applied at the same time. Ultrasonic spray gun was attached on top of the vertical type furnace. The droplet was sprayed into reaction zone of the furnace to form SCP which produces spherical shape with soft agglomerate crystalline ZnO particles. To characterize formed particles, fuel and oxidizing agent for SCP were used glycine and zinc nitrate or zinc hydroxide. Respectively, with changing combustion temperature and mixture ratio of oxidizing agent and fuel, the best ultrasonic spray conditions were obtained. To observe ultrasonic spray effect, two types of powder synthesis processes were compared. One was directly sprayed into furnace from the precursor solution (Type A), the other directly was heated on the hot plate without using spray gun (Type B). Powder obtained by type A was porous sponge shape with heavy agglomeration, but powder obtained using type B was finer primary particle size, spherical shape with weak agglomeration and bigger value of specific surface area. 9/ This can be due to much lower reaction temperature of type B at ignition time than type A. Synthesized nanocrystalline ZnO powders at the best ultrasonic spray conditions have primary particle size in range 20~30nm and specific surface area is about 20m$^2$/g.