• 한국균학회지
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• 제37권2호
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• pp.198-200
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• 2009

From 2008 to 2009, the stem rot of Astragalus sinicus L. caused by Sclerotium rolfsii occurred sporadically in Gyeongnam area, Korea. The typical symptom is water-soaking, rotting and wilting on the stem. The infected plants were eventually died. White mycelial mats were spread over lesions, and then sclerotia were formed on stems and near soil line. The sclerotia were globoid in shape, white to brown in color, 1-3 mm in size and the hyphal width was 3-9 μm. The optimum temperature for mycelial growth and sclerotial formation on PDA was 30oC. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the stem rot of A. sinicus caused by S. rolfsii in Korea.

• 식물병연구
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• 제21권4호
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• pp.334-336
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• 2015

2013년 7월 경상남도 진주시농업기술센터 양묘장 전시포에 재배중인 할미꽃에서 흰비단병 증상이 발생하였다. 병징은 할미꽃의 꽃줄기와 꽃자루 부분이 수침상으로 물러지면서 부패되어지고 서서히 시들면서 포기 전체가 말라 죽었다. 병반부와 토양 표면에 흰색의 곰팡이가 발생하며 갈색의 작은 둥근 균핵이 많이 형성되었다. 감자한천배지에서 균총은 흰색이고 배양 기간이 경과됨에 따라 갈색의 작은 둥근 균핵을 많이 형성하였다. 균핵의 크기는 1-3 mm이며 균사의 폭은 $4-9{\mu}m$였다. 균사생육과 균핵 형성 적온은 $30^{\circ}C$이었다. 균사특유의 clamp connection이 관찰되었다. 할미꽃에서 발생한 병징과 병원균의 균학적 특징, 그리고 ITS rDNA 염기서열 비교분석 결과, 이 병을 S. rolfsii Saccardo에 의한 할미꽃 흰비단병으로 명명하고자 제안한다.

• Mycobiology
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• 제30권4호
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• pp.244-246
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• 2002

A destructive stem rot of tomato(Lycopersicon esculentum) occurred sporadically some farmers' fields in Jinju City, Gyeongnam province in Korea. The infected plants also showed stem, crown rot or whole plant blight. White mycelium spread over stems of infected plants and formed sclerotia on the old lesions nearby soil surface. The fungus showed maximum mycelial growth around $30^{\circ}C$. The fungus formed white colony on PDA, usually with many narrow mycelial strands in the aerial mycelium and the width were $4.0{\sim}9.8{\mu}m$. The typical clamp connections were formed on the mycelium. Numerous sclerotia was formed on PDA at $30^{\circ}C$. The shape of sclerotia was globoid and $1.0{\sim}3.0$ mm in size. The fungus was isolated repeatedly from the infected tissues and the pathogenicity was confirmed to tomato and identified as Sclerotium rolfsii. This is the first report on the stem rot of tomato caused by S. rolfsii in Korea.

• Mycobiology
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• 제32권2호
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• pp.95-97
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• 2004

In July 2002, a destructive stem rot of tawny daylily(Hemerocallis fulva) was occurred sporadically in exhibition farm of Gyeongsangnam-do Agricultural Research and Extension Services located in Hamyang-gun, Korea. The fungus also caused collar and crown rot, and systemic wilt or blight of whole plant. White mycelium spread over stems and petioles of infected plants and sclerotia were formed on the old lesions and near the soil surface. The optimum temperature for mycelial growth and scierotial formations was $30^{\circ}C$ on PDA. The mycelial width ranged $4.2{\sim}10.4{\mu}m$ and the color was white, usually many narrow mycelial strand grew in the aerial mycelium and formed clamp connection. The shape of sclerotia was spherical and $1.0{\sim}3.2$ mm in diameter. The fungus was isolated repeatedly from the infected tissues and confirmed its pathogenicity to Hemerocallis fulva and identified as Sclerotium rolfsii. This is the first report on the stem rot of H. fulva caused by S. rolfsii in Korea.

• The Plant Pathology Journal
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• 제20권2호
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• pp.103-105
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• 2004

A destructive stem rot of strawberry (Fragaria x ananassa cv. Akihime) sporadically occurred in farmers' fields in Daegok-myon, Jinju city, Gyeongnam province in Korea. The infected plants showed stem and crown rot, with occasional blighting of the whole plant. White mycelia appeared on stems of infected clones and sclerotia formed on the old lesions near soil surface. The fungus formed white colony on PDA and showed maximum mycelial growth and sclerotial formation at $30^{\circ}C$. The fungus usually have many narrow hyphal strands, 2.6-10.0 $\mu\textrm{m}$ in width, in the aerial mycelium. Typical clamp connections were formed on the mycelium. Sclerotia were spherical and 1.0-2.4 mm in size. The fungus was repeatedly isolated from infected tissues and identified as Sclerotium rolfsii. Its patho-genicity was confirmed when inoculated onto straw-berry. This is the first report on the stem rot of strawberry caused by S. rolfsii in Korea.

• The Plant Pathology Journal
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• 제24권1호
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• pp.90-92
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• 2008

In 2007, a bulb rot of Allium victorialis var. platyphyllum Makino caused by Sclerotium rolfsii occurred sporadically in a herb farm at Gyeongsangnam-do, Korea. The symptom initiated with water-soaking lesion and progressed into bulb rot and wilt of a whole plant. Severely infected plants were blighted and killed eventually. White mycelial mats appeared on the lesion at early stage and a number of sclerotia were formed on the bulb. The sclerotia were spherical, $1{\sim}3 mm$ and white to brown. The optimum temperature for the growth and sclerotia formation was $30^{\circ}C$ on PDA. The hyphal width was measured $4-9{\mu}m$. The typical clamp connections were observed on PDA. Based on the symptom, mycological characteristics and pathogenicity to the host plant, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the bulb rot of A. victorialis var. platyphyllum caused by S. rolfsii in Korea.

• Mycobiology
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• 제30권2호
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• pp.102-104
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• 2002

In July 2001, a destructive stem rot of tatarian aster(Aster taturicus) was occurred sporadically in exhibition farm of Gyeongsangnam-do Agricultural Research and Extension Services, in Hamyang, Korea. The fungus also caused collar and crown rot and systemic wilt or blight of whole plant. White mycelium spread over stems and petioles of infected plants and sclerotia formed on the old lesions and near the soil surface. The fungus showed maximum mycelial growth was obtained around $30^{\circ}C$ but did not grow below $5^{\circ}C$ or above $45^{\circ}C$. The mycelial width ranges $4.2{\sim}10.4{\mu}m$. and the color is white, usually many narrow mycelial stand grow in the aerial mycelium and formed clamp connection. Numerous sclerotia were formed in artificial media like PDA at $30^{\circ}C$. The shape of sclerotia were sphere and $1.0{\sim}3.2{\mu}m$ in diameter. The fungus was isolated repeatedly from the infected, tissues and confirmed its pathogenecity to aster and identified as Sclerotium rolfsii. This is the first report that Sclerotium rolfsii causes stem rot of tatarian aster in Korea.

• 식물병연구
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• 제10권1호
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• pp.21-24
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• 2004

2002년 경남 하동군 진교면 노지재배 포장과 2003년 진주시 문산읍 촉성재배 비닐하우스 고추에서 이상증상이 발생하였다 병징은 지제부의 줄기가 수침상으로 물러지고 부패하면서 암갈색으로 변하고 시들면서 말라 죽는다. 병반부에 흰색의 곰팡이가 솜털처럼 생기고, 병반 부위와 토양 표면에 갈색의 둥근 균핵이 많이 형성된다 병원균의 균사생장 및 균핵형성은 감자한천배지에 배양시 3$0^{\circ}C$에서 가장 양호하였다. 균핵은 갈색 또는 암갈색으로 크기는 1.0∼2.7 mm이었다. 균사 특유의 clamp connection이 관찰되었고 균사 폭은 3.6∼10.2 $\mu\textrm{m}$이었다. 본 병원균을 Sclerotium rolfsii로 동정하였으며 고추 흰비단병으로 명명할 것을 제안한다.

• 식물병연구
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• 제11권1호
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• pp.85-87
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• 2005

2003년 6월에 경상남도 농업기술원 함양 약초시험포장에 재배중임 섬쑥부쟁이에서 Sclerotium rolfsii에 의한 흰비단병이 발생되였다. 병징은 줄기와 지제부위가 수침상으로 물러지고 부패하면서 암갈색으로 변하고 그 위에 흰색의 곰팡이가 솜털처럼 생기고, 병반부와 지제부위의 토양 표면에 갈색의 둥근 균핵을 많이 형성되었다. 병원균은 감자한천 배지상에서 왕성하게 생장하였으며 균사생장 중에 특유의 clamp connection이 관찰되었다. 균사의 크기는 3~$8\mu\textrm{m}$이었다. 감자한천 배지에서 갈색을 띤 구형 또는 부정형의 많은 균핵을 형성하였는데 균핵의 크기는 1~3mm였다. 균사생육 적온은 $30^{\circ}C$였다. 이상과 같이 병징과 균학적 특징 및 병원성을 검정한 결과, 이 병을 Sclerotium rolfsii에 의한 섬쑥부쟁이 흰비단병으로 명명하고자 한다.

• 식물병연구
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• 제9권1호
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• pp.25-27
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• 2003

2002년 경상남도 진주시 도로변 화단에 재배중인 팬지에서 줄기가 수침상으로 물러지고 부패하는 증상이 관찰되었다. 이 병의 병징은 지제부가 암갈색으로 변하고 그위에 흰색의 곰팡이가 솜털처럼 밀생하며, 병반 부위뿐만 아니라 지제부의 토양 표면에도 갈색의 둥근 균핵이 많이 형성된다. 병원균의 균사생장 및 균핵형성은 PDA배지에 배양시 3$0^{\circ}C$에서 가장 양호하였다. 균사에는 특유의 clamp connection이 관찰되었고 균사 폭은 3.9~10.4 $\mu\textrm{m}$이었다. 균핵은 갈색 또는 암갈색으로 크기는 1.3~4.2 mm 이었다. 이상의 특징으로 이 병원균은 Sclerorium rolfsii로 동정하였으며 팬지 흰비단병으로 명명할 것을 제안한다.