• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.68-68
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• 2002

Positional cloning of hereditary deafness genes is a direct approach to identify molecules and mechanisms underlying auditory function. Nowadays many deafness genes are newly identified by finding the locus for the causative genes. Mutations at many different loci in humans and mice are known to cause hearing impairment. Mouse mutants exhibiting deafness may be useful in identifying some of genes involved. (omitted)

• Korean Journal of Clinical Laboratory Science
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• v.44 no.3
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• pp.112-117
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• 2012

Escherichia coli biofilm, reported to be produced in the human intestine causing a significant health risk, was successfully grown on transwell$^{(R)}$. This biofilm layer was identified by crystal violet staining and prepared for the in vitro E. coli biofilm system which can be used to screen for inhibitors. The biofilm formation did not show a change in transepithelial electrical resistance values. Furthermore, rhodamine 123 staining showed that the dye did not pass through the membrane once biofilm was formed.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.10a
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• pp.41.2-41
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• 1999

• Proceedings of the Korean Vacuum Society Conference
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• 2007.02a
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• pp.66-66
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• 2007

• Journal of Korean Elementary Science Education
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• v.25 no.3
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• pp.281-295
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• 2006

The purpose of this study was to analyze laboratory instructions used in elementary school science lessons by utilizing an analysis instrument for science laboratory instruction(AISLI). This analysis instrument was comprised of 3 elements; the aim of the laboratory activity, the interaction generated, and the inquiry process. There were also a total of 20 sub-categories and its validity was identified at 0.89 by four science educators. For the purposes of this study, 90 laboratory instructions were video-recorded, then transcribed. Laboratory instructions were analyzed by three analyst teams, and the inter-rater reliability within teams was checked through Pearson correlation, with a score of 0.91. The results of this study were as follows: the two principle aims of laboratory activity, namely, to acquire declarative knowledge and to increase attitudes toward science, were observed to be in evidence in 98.9% and 92.2% in laboratory instructions, and the levels of categories of the interaction and inquiry processes were situated at level-1. The implications of these results are that laboratory instructions currently in use do not place sufficient demands upon students' scientific thinking and are not at all conducive to student-centered activities. Therefore in order to remedy this situation, the usefulness of current science laboratory instructions need to be re-evaluated.

• Bulletin of the Korean Space Science Society
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• 2008.10a
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• pp.37.1-37.1
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• 2008

Space Science and Technology Laboratory at Kyung Hee University developed an 80cm Nasmyth telescope with the joint work of Space & Astronomy, Inc. It was set up at Muju county public astronomical observatory in Jeonlabuk-Do. Nasmyth focus system was selected for the telescope to use two focal points by a rotatable tertiary mirror. Focal ratios of the telescope are f10, f5 respectively. Support of the main mirror is made with Lasalle-system. This system uses 24-points in the back side of the mirror that are all resting on small counter-weights and side support is 10-points Boll link Flexible type with 2 Lasalle type. The mount is wheel & disk type Alt-Azimuth design using DC-servo motors. External high accuracy encoder has 47,600 sine-waves/rot. These encoders are used to make real-time corrections on all gearing errors.

• Proceedings of the Korean Society of Life Science Conference
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• 2006.09a
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• pp.117.1-117.1
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• 2006

• Fisheries and Aquatic Sciences
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• v.25 no.4
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• pp.231-242
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• 2022

Effect of different concentrations of hypotaurine (HTU) on melanosis and quality of shrimps during 10 d storage in ice were studied. During refrigeration, the total plate count and total volatile basic nitrogen of shrimps treated with 20 g/L HTU were the lowest, and the hardness and microstructure were the best. Moreover, the score of melanoses, pH and total bile acid of shrimps treated with 20 g/L HTU were also low. Sensory evaluation showed that HTU treatment could make the shelf life of shrimps 3-4 days longer compared with the control. Based on the above physical and chemical indexes, 20 g/L HTU showed great potential as a safe inhibitor in the treatment of shrimps' melanosis.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09a
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• pp.672.1-672.1
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• 2006

• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.1018-1025
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• 2016

Probiotics are considered as the best effective alternatives to antibiotics. The aim of this study was to characterize the probiotic potential of lactobacilli for use in swine farming by using in vitro evaluation methods. A total of 106 lactic acid bacterial isolates, originating from porcine feces, were first screened for the capacity to survive stresses considered important for putative probiotic strains. Sixteen isolates showed notable acid and bile resistance, antibacterial activity, and adherence to intestinal porcine epithelial cells (IPEC-1). One isolate, LR1, identified as Lactobacillus reuteri, was selected for extensive study of its probiotic and functional properties in IPEC-1 cell models. L. reuteri LR1 exhibited good adhesion to IPEC-1 cells and could inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells. L. reuteri LR1 could also modulate transcript and protein expression of cytokines involved in inflammation in IPEC-1 cells; the Lactobacillus strain inhibited the ETEC-induced expression of proinflammatory transcripts (IL-6 and TNF-α) and protein (IL-6), and increased the level of anti-inflammatory cytokine (IL-10). Measurement of the permeation of FD-4 showed that L. reuteri LR1 could maintain barrier integrity in monolayer IPEC-1 cells exposed to ETEC. Immunolocalization experiments showed L. reuteri LR1 could also prevent ETEC-induced tight junction ZO-1 disruption. Together, these results indicate that L. reuteri LR1 exhibits desirable probiotic properties and could be a potential probiotic for use in swine production.