• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.179-180
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• 2000

$\beta$-glucan은 단순한 영양 공급원 혹은 식이 섬유로서 뿐 만 아니라 면역 활성증강 등의 생리적 활성이 우수하여 기능성 식품 소재로 활용가능함이 보고되고 있다. $\beta$-glucan은 $\beta$-1,3 glucan의 구조를 기본으로 하여 $\beta$-1,4 혹은 $\beta$-1,6 등의 가지를 함유하고 있어 그 구조에 따라 생리 활성이 달라지는 것으로 알려져 있다. 현재 식품 소재로 이용하고 있는 $\beta$-glucan은 보리, 귀리, 효모 등에서 생산되고 있으나 대량 생산 및 분리, 정제 등의 어려움이 있다. (중략)

• Journal of Applied Biological Chemistry
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• v.64 no.1
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• pp.103-103
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• 2021

• Korean Journal of Food Science and Technology
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• v.34 no.1
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• pp.8-12
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• 2002

In order to artificially increase the contents of essential oils from 4 different trees by inducing with elicitors, 5 kinds of chemical elicitors and 4 kinds of biotic elicitors were selected. Before treatment, the contents of essential oils from Japanese Cypress (Chamaecyparis obtusa), Sawara cypress (Chamaecyparis pisifera), Japanese Red Pine (Pinus densiflora), and Korean Pine (Pinus koraiensis) were 2.0, 1.6, 0.4, and 0.7 percent, respectively, and the maximum content of essential oils from all species were reached in July. By most of elicitors, the essential oil content was generally increased after 6 months later, but some of elicitors did not affect the content of essential oil. Finally, the appropriate inducers for artificially increasing the essential oil were respectively selected depending on each species; Schizophyllum commune Fries for Japanese Red Pine and Japanese Cypress, hydrogen peroxide for Korean Pine, and ${\beta}-pyridone$ for Sawara cypress. Especially, hydrogen peroxide and ${\beta}-pyridone$ could be wide spread inducer for all 4 species.

• Mycobiology
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• v.36 no.1
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• pp.34-39
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• 2008

Schizophyllum commune is an edible and medicinal mushroom widely distributed in the world. The optimal growth conditions for the mycelia of 10 strains of the fungus were investigated. The temperature suitable for the mycelial growth and density was obtained at $30{\sim}35^{\circ}C$. Among the tested conditions, the minimum mycelial growth was found at $15^{\circ}C$. In case of pH, the most favorable growth was found at pH 5. The results indicated that this mushroom well adapted to high temperature and low pH for its mycelial growth. Considering growth phenotype of mycelia, Hamada, Hennerberg, PDA and YM were the most suitable and Lilly, Glucose triptone, Glucose peptone and Hoppkins were the most unfavorable among tested media for the mycelial growth of S. commune. Out of tested carbon sources, dextrin and fructose were the most suitable and lactose, mannose and sorbitol were the unsuitable for the fungus. Compact mycelial density was obtained from most of the carbon sources. Among used nitrogen sources, calcium nitrate, potassium nitrate and alanine were the most appropriate and the most incompatible were ammonium phosphate, histidine, urea and arginine for mycelial growth of S. commune on the culture media. Calcium nitrate, histidine and potassium nitrate showed moderately thin or thin, and rest of nitrogen sources showed compact or moderately compact mycelial density.

• The Korean Journal of Mycology
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• v.22 no.3
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• pp.247-253
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• 1994

This study was carried out to isolate and characterize $A{\alpha}$ mating locus controlling fruiting body formation directly in the Basidiomycete Schzophyllum commune growing in the North America. Total numbers of genomic library of S. commune UVM1-34 was about $2{\times}10^4$ cells. About 90% library was appeared to have about 35 kb inserted genome DNA in cosmid pTC20 vector. 6 clones were proved to have positive signal to probes within Z and Y region in colony and southern hybridization. In the mating activity test, all the 6 positive clones were appeared to have $A{\alpha}3$ mating activity although they had two different restriction patterns. pSC13 containing 5.7 Kb PstI-fragment of UVM 1-34 $A{\alpha}3$ allele showed about 50% clamp cell formation indicating mating activity when cotransformation was done together with cosmid pTC20.

• The Korean Journal of Mycology
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• v.42 no.4
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• pp.269-275
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• 2014

Halophytes of seven species, Carex scabrifolia Steud., Limonium tetragonum Bullock, Salicornia europaea L., Suaeda glauca Bunge, Suaeda japonica Makino, Suaeda maritima Dumort., and Triglochin maritimum L. were collected from the Taean Peninsula. Thirty-seven endophytic fungi were isolated from the root of halophytes, and analyzed using the DNA sequences of internal transcribed spacers (ITS). The diversity of all endophytic fungi was analyzed using diversity indices. The endophytic fungi associated with the halophytes belonged to seven orders; Eurotiales (78%), Capnodiales (5%), Hypocreales (5%), Agaricales (3%), Corticiales (3%), Glomerellales (3%), and Pleosporales (3%). At the phylum level, the endophytic fungi were composed of Ascomycota and Basidiomycota. At the genus level, Alternaria, Aspergillus, Cladosporium, Paecilomyces, Penicillium, Phanerochaete, Schizophyllum, Talaromyces, and Verticillium were confirmed. Among them, Penicillium was the most abundant in the roots of the halophytes. This study analyzed the distribution and diversity of endophytic fungi on halophytes in the Taean Peninsula.

• The Plant Pathology Journal
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• v.36 no.6
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• pp.579-590
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• 2020

Botrytis cinerea, which causes gray mold disease in more than 200 plant species, is an economically important pathogen that is mainly controlled by synthetic fungicides. Synergistic fungicide mixtures can help reduce fungicide residues in the environment and mitigate the development of fungicide-resistant strains. In this study, we screened microbial culture extracts on Botrytis cinerea to identify an antifungal synergist for tebuconazole. Among the 4,006 microbial extracts screened in this study, the culture extract from Schizophyllum commune displayed the most enhanced activity with a sub-lethal dosage of tebuconazole, and the active ingredient was identified as schizostatin. In combination with 5 ㎍/ml tebuconazole, schizostatin (1 ㎍/ml) showed disease control efficacy against gray mold on tomato leaf similar to that achieved with 20 ㎍/ml tebuconazole treatment alone. Interestingly, schizostatin showed demethylation inhibitor (DMI)-specific synergistic interactions in the crossed-paper strip assay using commercial fungicides. In a checkerboard assay with schizostatin and DMIs, the fractional inhibitory concentration values were 0.0938-0.375. To assess the molecular mechanisms underlying this synergism, the transcription levels of the ergosterol biosynthetic genes were observed in response to DMIs, schizostatin, and their mixtures. Treatment with DMIs increased the erg11 (the target gene of DMI fungicides) expression level 15.4-56.6-fold. However, treatment with a mixture of schizostatin and DMIs evidently reverted erg11 transcription levels to the pre-DMI treatment levels. These results show the potential of schizostatin as a natural antifungal synergist that can reduce the dose of DMIs applied in the field without compromising the disease control efficacy of the fungicides.

• Journal of the Korean Wood Science and Technology
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• v.48 no.1
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• pp.50-60
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• 2020

Fungi are wood-decaying organisms, and this is an important trait that should be considered in wood utilization. When fungi attack wood, it decreases the quality of the wood. The use of metal screws has become an important part of woodworking. The ability of fungi to decay wood and damage metal screws that are embedded into wood is varied. In this study, eight fungal species were evaluated with respect to their ability to decay Parashorea smythiesii and P. tomentella wood. In addition, the effect of fungi on corroding metal screws was determined using the Kolle flask method. The evaluation showed that the fungal species Schizophyllum commune, Pycnoporus sanguineus, and Polyporus arcularius were highly capable of decaying Parashorea spp. woods. The greatest wood weight loss occurred with the heartwood of P. tomentella exposed to S. commune. Based on the classification of wood resistance against fungal attack, the two Parashorea spp. were classified as moderately resistant woods (class III). Schizophyllum commune was classified as highly capable of decaying wood that was embedded with metal screws and was highly capable of corroding metal screws placed in fungi-culture media. The greatest weight of rust powder formed because of screw corrosion was obtained from screw-embedded wood exposed to S. commune. Additionally, the most severe corrosion of metal screws that were embedded into woods was caused by the activities of P. arcularius. Moreover, the average weight loss of screw-embedded wood was greater than that of unscrewed wood.

• Mycobiology
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• v.31 no.1
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• pp.42-45
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• 2003

For transformation of Pleurotus ostreatus, two novel vectors, pPhKM1 and pPhKM2, were constructed, using the regulatory sequences of the P. sajor-caju $\beta$-tubulin gene(TUB1) and the ble gene encoding phleomycin binding protein. pPhKM1 contains ble fused to the TUB1 promoter and the Schizophyllum commune GPD terminator. pPhKM2 contains ble fused to the promoter and terminator regions of P. sajor-caju TUB1. To confirm phleomycin-resistance activity, each vector was cotrans-formed with pTRura3-2 into the P. ostreatus homokaryotic $ura^-$ strain. The transforming DNA was stably integrated into the genomic DNA. Subsequently, phleomycin resistance was conferred on wild-type dikaryotic P. ostreatus by transformation with pPhKM1 or pPhKM2. This transformation system generated stable phleomycin-resistant transformants.

• Mycobiology
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• v.38 no.3
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• pp.189-194
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• 2010

This study investigated the cultural characteristics of Shimizuomyces paradoxus in different nutritional and environmental conditions. The highest mycelial growth was observed in Schizophyllum (mushroom) genetics complete medium plus yeast extract agar medium, and the optimal temperature and pH were $25^{\circ}C$ and pH 8.0, respectively. The optimal carbon and nitrogen sources were 1% dextrose and 1% peptone in agar. However, in liquid culture the highest dry mycelium weight was found for the potato dextrose agar and potato sucrose agar broths. The optimum inoculum size was five mycelial discs (5 mm) per 100 mL of broth, and the optimum liquid culture period was 25 days. This is the first ever report of S. paradoxus cultural characteristics.