• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 1998년도 추계 학술대회 발표 논문집
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• pp.68-68
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• 1998

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 1999년도 춘계학술대회 발표논문집
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• pp.77-77
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• 1999

• 한국미생물·생명공학회지
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• 제42권2호
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• pp.131-138
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• 2014

While coupling wastewater treatment with microalgal bioenergy production is very promising, new approaches are needed to enhance microalgal growth and lipid accumulation in wastewater. Therefore, this study investigated the effect of iron on the growth, nutrient removal, and lipid accumulation of Scenedesmus sp. LX1 in both artificial wastewater and domestic secondary effluents. When increasing the iron concentration from 0 to 2 mg/l in the artificial wastewater, the biomass production of Scenedesmus sp. LX1 increased from 0.17 to 0.54 g/l; the nitrogen and phosphorus removal efficiency increased from 15.7% and 80.6% to 97.0% and 99.2%, respectively; and the lipid content was enhanced 84.2%. The relationship between the carrying capacity/maximal population growth rate of Scenedesmus sp. LX1 and the initial iron concentration were also in accordance with the Monod model. Furthermore, when increasing the iron concentration to 2 mg/l in four different domestic secondary effluent samples, the lipid content and lipid production of Scenedesmus sp. LX1 was improved by 17.4-33.7% and 21.5-41.8%, respectively.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2000년도 추계심포지움 및 학술발표회:바이오모니터링 기법을 이용한 환경위해성 평가
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• pp.77-77
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• 2000

• KSBB Journal
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• 제25권4호
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• pp.363-370
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• 2010

Lipid from Scenedesmus sp. was extracted, fractionated and purified by silicic acid column chromatography. Total lipid content of extract was $21.38{\pm}1.03$ wt%, and triacylglycerol, an index lipid for biodiesel production was detected. Ten species of $C_{16}{\sim}C_{22}$ with saturated and unsaturated fatty acid were identified showing the very adequate fatty acid profiles for biodiesel production with a good flow property at low temperature. The fractions of glycolipid, neutral and phospholipid were 52.64, 28.10 and 19.26% of total lipid, respectively. The triacylglycerol of 12.63% and chlorophyll a of 49.47% was fractioned using stepwise elution of n-hexane-diethyl ether (95:5, v/v) solvent. The high content of chlorophyll was considered as a potential source of value-added co-product.

• 미생물학회지
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• 제6권1호
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• pp.12-21
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• 1968

The accelerate effects on the growth rate and the capacity of fermentation of L. delbrukii and B. subtilis was investigated in the Henneberg's medium added by various amounts of cellular components of Chlorella and Scenedesmus and also was investigated in the media added micronutritional elements such as Mn, Fe and Mo, etc. The results in the comparative experiments are as follow; 1. Various amounts of Chlorella cell components in the media accelerated remarkably the lactic acid formation and growth of L. delbrukii. For example, lactic acid formation in the medium of contained 1 percent Chlorella cell components was promoted more than twice effects compare with control. 2. The formation of .alpha.-amylase by B. subtilis in the medium of 2 percent Chlorella cell contents was also promoted more than nine twice effects compare with control. 3. The formation of lactic acid of L. delbruckii in the medium of Scenedesmus cell contents was a little more than in the medium of Chlorella cell contents. 4. The lactic acid fermented level attained with the addition of 0.2-0.25 percent Chlorella cells was the effect of promoting fermentation attained of saturating level at 100$\mu$g. /ml. of Mn and 0. 1 $\mu$g./ml. of Fe.

• 상하수도학회지
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• 제29권3호
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• pp.319-323
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• 2015

This study investigated the effects of the light conditions on the productivity of scenedesmus dimorphus in the continuous mass cultivation system. To compare the algal productivity according to the light conditions, S. dimorphus was cultivated continuously under the wide range of light intensity(200-600 PPFD) and various light wavelength(white light and red-blue mixed light). After 100 days of cultivation under the different light intensity, the productivity of S. dimorphus increased as light intensity decreased. So, the productivity was maximized as 100 mg/L/d when light intensity was 200 PPFD. In case of light wavelength, the productivity of S. dimorphus was enhanced about 20% with the white light compared to that of the red-blue mixed light. Consequently, the optimal light conditions for the continuous mass cultivation of S. dimorphus were 200 PPFD as light intensity and white light as light wavelength.

• 한국농공학회논문집
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• 제53권1호
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• pp.63-69
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• 2011

The green algae Scenedesmus acuminatus was cultured in different media: animal wastewater and an artificial culture medium in order to evaluate potential use for tertiary treatment. The experiments were conducted with air flowrate 1~2 L/min at $28{\sim}30^{\circ}C$. The nitrogen and phosphorus showed very similar removal efficiencies (68~77 % and 69~80 % for nitrogen and phosphorus respectively). The optimal fed period was estimated as three days in the semi-continuous experiment. The effects of $CO_2$ (4.5 %) injection on nutrient uptake from animal wastewater (biological treatment effluent) were compared to an air injection under the same conditions of light and photoperiod. The uptake rates of nutrient with air injection were observed 0.009 gN/gChl-a/day, 0.028 gN/gChl-a/day and T-P 0.003 gP/gChl-a/day for nitrate, total nitrogen and phosphorus respectively. The rates were enhanced by addition of $CO_2$ to 0.026 gN/gChl-a/day, 0.076 gN/gChl-a/day and T-P 0.018 gP/gChl-a/day. This study establishes that $CO_2$ addition during nutrient deprivation of microalgal cells may accelerate tertiary wastewater treatment.

• 한국환경보건학회지
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• 제23권1호
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• pp.18-27
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• 1997

The isolation, morphological study and growth characteristics of the algae were investigated from Lake Chuam. The isolated algae were applied the Agal Growth Potential test. The method of isolation and purification of the algae were used to Agar plating(AP), nutrient enrichment(NE), dilution(DI) and micro capillary technique(MC). Total isolated algae were 21 species. They were composed of Cyanophyceae, Dinophyceae, Bacillariophyceae, Euglenophyceae and Chlorophyceae. The numbers of algal strain by isolation technique were highest in dilution(21 species), and those of the rests were showed in order of NE > MC > AP. The sizes of isolated Selenastrum and Scenedesmus were $1.8\pm 1.4 \mu m$, $3.3\pm 0.9 \mu m$ in diameter and $6.4\pm 2.3 \mu m$, $13.6\pm 1.9 \mu m$ in length respectively. The morphology of isolated algae and NIES-collection strain was very similar each other, but the size was smaller isolated algae than that of NIES-collection. The optimum culture condition of isolated Selenastrum and Scenedesmus was about 30$\circ$C(25$\circ$C-35$\circ$C) in temperature and the maximum growth was appeared between 7,000 lux and 8,000 lux in the light intensity. The comparison of $\mu$(specific growth rate) on the concentration of nutrients such as nitrate and phosphate, isolated Selenastrum was appeared maximum it at 1.0 mg $NO_3-N/l$ but NIES-collection strain was showed 95% of maximum it at same nitrate concentration. Maximum g of isolated algae and NIES-collection strain in Scenedesmus onto nitrate concentration were very similar with the result of selenastrum. The specific growth rates of isolated algae and NIES-collection strain on the gradient concentration of phosphate were showed 0.72/day and 0.70/day at 0.02 mg $PO_4-P/l$ in Selenastrum but those of Scenedesmus were appeared 0.61/day and 0.57/day at same concentration $PO_4-P$.

• 생태와환경
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• 제34권4호통권96호
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• pp.285-291
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• 2001

상위포식자에 의해 유도되는 Scenedesmus dimorphus (T$\ddot{u}$rpin) K$\ddot{u}$tzing의 군체형성에 관한 실험을 수행하였다. 동물플랑크톤 Daphnia magna (300 ind./L)와 Moina macrocopa (500 ind./L) 배양한후, $0.1\;{\mu}m$ (millipore)로 여과하여 얻어낸 용액 (ZFW)을 S. dimorphus가 1.8 ($10^5\;cells/ml$까지 자란 처리군에 첨가하여, ZFW를 넣지않은 대조군과 비교하였다. 대조군에 비해 두 동물플랑크톤 처리군에서 S. dimorphus의 군체형성의 유도가 뚜렷이 관찰되었다. 이 현상은 M. macrocopa 보다는 D. magna처리군에서 뚜렷하게 관찰되었으며, 첨가해준 ZFW의 양이 증가할수록 particle당 세포수도 비례적으로 증가하는 것으로 나타났다. ZFW처리군에서 1군체당 세포수(cells/colony)와 평균체적 (mean particle biovolume)은 $24{\sim}72$시간 사이에 급격히 증가하는 것으로 관찰되었다. S. dimorphus의 군체형성현상은 동물플랑크톤 D. magna와 M. macrocopa의 분비물에서 유도되는 화학물질의 영향으로 보이며, 이와 같은 형태변화현상은 동물플랑크톤의 포식에 대한 방어기작으로 작용하게 될 것으로 사료된다.