• Korean Journal of Environmental Agriculture
• /
• v.36 no.4
• /
• pp.241-248
• /
• 2017

BACKGROUND: RNA interference (RNAi) eliminates or decreases gene expression by disrupting the target mRNA or by interfering with translation. Recently, RNAi technique was applied to generate new crop traits which provide protection against pests. To establish the environmental risk assessment protocol of RNAi LMO in lab scale, we developed dsRNA expression system using E. coli and tested acute oral toxicity assay to honey. METHOD AND RESULTS: The dsRNA expression vector, L4440, was chosen and cloned 240 bp of Snf7 and GFP gene fragment. To develop the maximum dsRNA induction condition in E. coli, we tested induction time, temperature and IPTG concentration in media. To estimate the risk assessment of dsRNA to honey bee, it has been selected and cultured with dsRNA supplement for 48 hours according to OECD guideline. As a result, the optimum condition of dsRNA induction was $37^{\circ}C$, 4 hours and 0.4 mM IPTG concentration and the difference between Snf7 and GFP dsRNA molecules from E. coli was not significant in survival and behavior to honey bee. Furthermore, blast search results indicated that effective match of predicted dsRNA fragments were not existed in honey bee genome. CONCLUSION: In this study, we developed and tested the acute oral toxicity of dsRNA using E. coli expression system to honey bee.

• Journal of Internet Computing and Services
• /
• v.17 no.3
• /
• pp.75-85
• /
• 2016

Changes in the distribution and sharing information using the Internet is changing the most part in our daily life, Utilizing the Internet of our lives to the rapid development of IT technology, are living in the off-line (off-line) and online (on-line) a mix of life, We expect now online life specific gravity is further increased. However, do not systematically implement the education of Internet ethics awareness deficiencies of objective indicators to assess the current knowledge level of the Internet ethics is the actual situation. Therefore, in this paper, it provides a development process in order to establish a common standard for the evaluation of Internet ethics index. Development procedure of Internet ethics index in order to establish a common standard, "evaluation item (draft) derivation", "Creating a questionnaire", "the calculation of the analysis and item-by-item weight of the questionnaire", and a common reference determination of the stage having. Was also a questionnaire that target 46 people to calculate the weight of the common reference. Internet ethics index evaluation model through the establishment of the common standards developed in this paper, municipal road Office of Education, related organizations, high school, such as small, medium, can be utilized as an indicator to measure the Internet ethics education level.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.37 no.2C
• /
• pp.206-216
• /
• 2012

Today's war fields can be characterized by net-centric wars where a variety of independent weapon systems are operated in connection with each other via networks. As such, weapon systems become dramatically advanced in terms of complexity, functionality, precision and so on. It is then obvious that the defense R&D of those requires systematic and efficient development tools enabling the effective management of the complexity, budget/cost, development time, and risk all together. One viable approach is known to be the development methods based on systems engineering, which is already proved to successful in U.S. In this paper, a systems engineering approach is studied to be used in the conceptual design of advanced weapon systems. The approach is utilizing some graphical models in the design phase. As a target system, an unmanned aerial vehicle system is considered and the standard SysML is also used as a modeling language to create models. The generated models have several known merits such as ease of understanding and communication. The interrelationships between the models and the design artifacts are identified, which should be useful in the generation of some design documents that are required in the defense R&D. The result reported here could be utilized in the further study that can eventually lead to a full-scale model-based systems engineering method.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.45 no.1
• /
• pp.82-93
• /
• 2017

The purpose of this study is analyze the impact on users' cognition of design concept, satisfaction of design concept, adapted park space, and effect of design concepts on park use satisfaction. The study sites are Central Mountain Park and Lake Park in Gwanggyo New Town, Suwon. We analyzed importance-satisfaction of park facilities in the park and preference-satisfaction of park. The results of this study are as follows. First, generally users visit the park as a family unit. Second, trails got high scores in importance-satisfaction of park facilities. Third, park users' satisfaction received a high score generally, but it doesn't show statistic significance with users' cognition of design concept. Fourth, cognition of the two parks' design concept and design strategy got a high score - over three points on average - based on the 5 - point Likert scale. However, upon analysis of users' cognition of design concept and satisfaction of design concept adapted park space, there is no statistic significance. Therefore, we noticed that the park's design concept had an effect on park users' satisfaction; rather, personal preference and satisfaction of users had more impact on satisfaction. On the other hand, this study was limited in that we didn't survey across all four seasons and had few target areas. But it has some significance in that we used a visual documentation, attaching existing photos including design concept, to increase users' understanding. Also, we directly asked about the parks' user design concept and strategy.

• Solar Energy
• /
• v.12 no.3
• /
• pp.10-20
• /
• 1992

In this experiment, photochemical reaction has been applied to destroy TCE in water phase. The main target of this work is to investigate the technical feasibility of large scale of solar detoxification reactor for water treatment. The results have revealed that solar detoxification utilizing photon energy from the sun is the most attractive process to decompose organic toxins in water phase at room temperature. The detailed results from this work are as follows; (1) The highest conversion ratio of TCE was obtained by using $TiO_2$, annatase as a photocatalyst among $TiO_2$ anatase, $TiO_2$ rutile and $V_2O_5$ under the same experimental condition. The anatase crystal structure was confirmed with XRD analysis, and its surface area was 7.748 $m^2/g$ from the BET-$N_2$ measurement (2) 0.1 wt% of $TiO_2$ anatase has been adopted as optimal quantity for batch slurry reactor at this experimental conditions. (3) The effect of hydrogen peroxide on the conversion of TCE was investigated. Its optimal quantity was 0.06 vol. % under this experimental conditions. (4) The effect of oxygen on the conversion of TCE also was studied by controlling the head space in photoreactor. Results indicated that sufficient amount of oxygen should be supplied to accomplish the highest conversion rate of TCE in water phase.

• Journal of the Institute of Electronics and Information Engineers
• /
• v.50 no.5
• /
• pp.83-93
• /
• 2013

Recently, the SURF algorithm, which is conjugated for object tracking system as part of many computer vision applications, is a well-known scale- and rotation-invariant feature detection algorithm. The SURF, due to its high computational complexity, there is essential to develop a hardware accelerator in order to be used on an IP in embedded environment. However, the SURF requires a huge local memory, causing many problems that increase the chip size and decrease the value of IP in ASIC and SoC system design. In this paper, we proposed a way to design a SURF algorithm in hardware with greatly reduced local memory by partitioning the algorithms into several Sub-IPs using external memory and a DMA. To justify validity of the proposed method, we developed an example of simplified object tracking algorithm. The execution speed of the hardware IP was about 31 frame/sec, the logic size was about 74Kgate in the 30nm technology with 81Kbytes local memory in the embedded system platform consisting of ARM Cortex-M0 processor, AMBA bus(AHB-lite and APB), DMA and a SDRAM controller. Hence, it can be used to the hardware IP of SoC Chip. If the image processing algorithm akin to SURF is applied to the method proposed in this paper, it is expected that it can implement an efficient hardware design for target application.

• Composites Research
• /
• v.28 no.4
• /
• pp.212-218
• /
• 2015

A large composite spar was manufactured using an automatic fiber placement (AFP) machine. To verify its structural performance, the weakest part of the structure, which is called 'corner radius', was tested under bending and examined by finite element analysis. Since the application of AFP machine to composite structure fabrication is still in early stage in Korea, this paper presents the summary of whole process for manufacturing composite spar using AFP machine from mandrel design and analysis to verification test. The deflection and stress by mandrel weight and AFP machine force, thermal deformation and natural frequency were all examined for mandrel design. The target structure was composite C-spar and cured in an autoclave. Test results were compared with nonlinear finite element analysis results to show that the structure has the strength close to the theoretical value. It was confirmed that the corner radius of the spar manufactured by AFP process showed deviation less than 20% compared with first ply failure strength. The results indicate that the AFP technology could be used for large scale composite structure production in the near future.

• The Journal of Korean society of community based occupational therapy
• /
• v.8 no.1
• /
• pp.23-31
• /
• 2018

Objective : Purpose of this study was to investigate the effect of depression and self - efficacy on the quality of life of the elderly hospitalized in a nursing hospital and to suggest a new direction for improving the quality of life of the elderly. Methods : The target was 212 senior citizens who were hospitalized at a nursing hospital located in K city in North Gyeongsang Province. The questionnaire was structured by adding the characteristics of the subjects, the Korean Senior Depression Index (GDS-K), the Self-effective Index (WHQOL-BRFA), and the World Health Organization's Life-Enhanced scale. coded using SPSS ver.18, the depression, self-efficiency, and quality of life differences according to the general characteristics (t-test, ANOVA), and Self-efficacy, depression, and the correlation coefficient between quality of life and A multI-sentence analysis to see the impact of depression and self-efficacy on the quality of life. Results : Self - efficacy and depression according to subject characteristics were different according to length of stay, and quality of life was different in religion. The correlation between depression, self - efficacy, and quality of life of admitted elderly showed statistically significant negative correlation (p <.01) with depression in both the quality of life and self - efficacy sub - variables. The depression and the self - efficacy of the elderly had the greatest effect on the quality of life. The variables were depression (${\beta}=-.328$), social efficacy (${\beta}=.248$), and physical efficacy (${\beta}=.193$). Conclusion : In order to improve the quality of life of the elderly, it will be necessary to provide and develop medical care services that reduce depression and improve self - efficacy (physical and social).

• Journal of Radiation Protection and Research
• /
• v.30 no.4
• /
• pp.237-245
• /
• 2005

The distribution of radionuclides in soil and plants were calculated, assuming an accidental release of radionuclides from Yeonggwang Nuclear Power Plant. The results which show the concentration change with time and regions were displayed by GIS. GIS Included the commercial program, ArcView(ESRI), and a basic digital map of 1:5000 scale for 30km by 30km area around Yeonggwang Nuclear Power Plant. The target material was $^{137}Cs$ in soil around Yeonggwang area. Given denosited $^{137}Cs$ concentrations, ECOREA-II code computed the $^{137}Cs$ concentration of the soil and the plant in the area divided by 16 azimuth, 480 unit cells in total in which the concentrations also varied with time. The results were introduced into the attributed data of previously designed polygon cells in ArcView. In order to display the concentration change with time by monotonic color, the RGB value for ArcView color lamp was controlled. This display is useful for the public to understand the concentration change of radionuclide around Yeonggwang area definitely.

• Resources Recycling
• /
• v.26 no.6
• /
• pp.73-83
• /
• 2017

Used small household appliances have a wide variety of product types and component materials, and contain high percentage of black plastics. However, they are not being recycled efficiently as conventional sensors such as near-infrared ray (NIR), etc. are not able to detect black plastic by types. In the present study, an automatic sorting system was developed based on laser-induced breakdown spectroscopy (LIBS) to promote the recycling of waste plastics. The system we developed mainly consists of sample feeder, automatic position recognition system, LIBS device, separator and control unit. By applying laser pulse on the target sample, characteristic spectral data can be obtained and analyzed by using CCD detectors. The obtained data was then treated by using a classifier, which was developed based on artificial intelligent algorithm. The separation tests on waste plastics also were carried out by using a lab-scale automatic sorting system and the test results will be discussed. The classification rate of the radial basis neural network (RBFNNs) classifier developed in this study was about > 97%. The recognition rate of the black plastic by types with the automatic sorting system was more than 94.0% and the sorting efficiency was more than 80.0%. Automatic sorting system based on LIBS technology is in its infant stage and it has a high potential for utilization in and outside Korea due to its excellent economic efficiency.