The methods that make Hantavirus grow consist of inoculation into the experimental animals and cultured cells. The cultured cells, such as Vero-E6 and A549 cells, have been usually used for isolation of the virus and the animals, such as mice and rats, are used for large scale preparation of the virus so far. Furthermore, the cell can be used to maintain the virus and assay the infectivity and the animals can be used for the experiment of viral pathogenicity and challenge for assessment of vaccine. Apodemus mice, the own natural host of the virus, has been used for challenge test of Hantaan virus. However it has been pointed out to difficult handling and breeding the animal in laboratory. Therefore, we attempted to establish a new animal model for challenge test at the time of isolation of Maaji virus which is a new hantavirus similar but distinct to Hantaan virus. In suckling hamster, the titer of Maaji virus and the lethality to mice of the virus were increased gradually in the titer and lethality through passage by intracerebral (IC) inoculation. We tried to re-adapt this brain virus to lung of weanling hamster. The brain passaged virus was inoculated into weanling hamster intramuscularly. Again, the titer of the virus in lung was also increased by continuous passage of this virus. This facts could regarded as adaptation to new environment in which the virus proliferates. To identity the virus passaged in hamster with Maaji virus, both of the virus passaged in hamster brain and lung were compared with Maaji virus (MAA-I) and Hantaan virus (HTN 76-118) by means of restriction fragment length polymorphism (RFLP) and slingle strand conformation polymophism (SSCP). As a result, we conclude that Maaji virus could be adapted successfully to weanling hamster through this passage strategy. Utilizing this adapted Maaji virus strain, hamster model is able to be used for challenge test in hantaviral vaccinology and further experiments utilizing hamster system as a rather available and convenient lab animal are expected.
Journal of the Korean Society of Environmental Restoration Technology
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v.17
no.1
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pp.135-146
/
2014
The improvement of adaptation capability against heat island (ACHI) by greening buildings is considered as an important measure to cope with a climate change. This study aimed to select the most appropriate zones for green roof initiative in case study sites, Bucheon, Anyang, and Suwon Cities and to investigate the characteristics of buildings for greening to improve ACHI. Relative ACHI for each lot was estimated from 0 to -9, assuming that it decreases with the distance from green space and waterbody. Low adaptation capabilities were mostly shown in the old urban blocks with dense low-rise buildings and lack of green space. Three blocks with the lowest ACHIs were chosen as a green roof initiative zone in each city. They are largely residential areas including low-rise buildings such as single, multi-household houses, townhouses, 5 or lower story apartments and few are industrial areas crowded with small factory buildings. The areas of building roof available for greening are 8.8% within the selected zones in Bucheon City, 5.3% in Anyang City, and 4.9% in Suwon City. As it were, 25.2~41.7% of the roof top areas are available for greening in these zones. It means that roof top areas of $25,000{\sim}120,000m^2$ can be used for greening within the selected zones of $0.64{\sim}1.65km^2$ to improve ACHI. The approach and results of the study are significant to provide a logical basis and information on location, scale, effect, and target figure of greening as a measure to cope with climate change.
International Journal of Internet, Broadcasting and Communication
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v.12
no.3
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pp.163-170
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2020
The purpose of this research is to organize panels centered on professional workers and experts in the sports media industry, identify structures and items of sports media commentators' attributes that conform to the present era through the collective agreement process, and evaluate the derived measures according to the scale development procedure. We would like to shed new light on the nature of commentators due to the rapid development of broadcasting technology and the emergence of new media platforms, the emergence and expansion of smart media receptors and the changing environment of modern sports broadcasting. For this purpose, a panel of experts from academia and industry related to sports management and sports media was organized and the attributes of commentators were analyzed using the Delphi method. Technology development in the sports market has created a new sports consumption environment. In these consumer environments, various environmental factors influence the consumer's consumption culture of sports consumers. For example, professional information, as well as interpretations of sports culture, history and society are becoming their share for sports commentators, and viewers are demanding more diverse characteristics from sports commentators. In this respect, the study of sports commentator attributes can be a deep understanding of the rapidly changing culture of sports consumption.
The Journal of Korean Academic Society of Nursing Education
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v.25
no.1
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pp.17-26
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2019
Purpose: This study was conducted to verify the validity and reliability of the modified Transition Shock Scale for use among nursing students. Methods: From March 8-April 3, 2018, 207 nursing students participated in this study and were asked to complete self-reported questionnaires, including transition shock, clinical stress, and adapting to clinical practicums. The IBM SPSS Win 18.0 and AMOS 18.0 programs were used for data analysis. Results: A confirmatory factor analysis supported good convergent and discriminant validities ($x^2/df=1.58$, TLI=.92, CFI=.95, RMSEA=.053, AVE=.51-.68, CR=.75-.68). Correlations among transition shock, clinical stress (r=.34, p<.001), and adapting to clinical practicums (r=-.54, p<.001) were significant. Cronbach's alpha for the overall scale was .85, and for the six subfactors it ranged from .65 to .75. Conclusion: The findings suggest that the 17-item transition shock questionnaire is an appropriate instrument for measuring nursing students' transition shock with good validity and reliability.
Purpose: This study aimed to develop and test a model for posttraumatic growth among cured patients with COVID-19. This model was based on Calhoun and Tedeschi's Posttraumatic Growth model and a literature review. Methods: The participants comprised 223 patients cured from COVID-19 who were ≥ 19 years of age. The data were collected through an online questionnaire from March 21 to 24, 2022. The assessment tools included the Impact of Event Scale: Revised Korean version, the Connor-Davidson Resilience Scale, the Distress Disclosure Index, the Multidimensional Scale of Perceived Social Support, the Korean version of the Event-related Rumination Inventory, and the Korean version of the Post-traumatic Growth Inventory. Data were analyzed using the IBM SPSS version 24.0 and IBM AMOS 26.0. Results: The modified model showed appropriate goodness of fit (χ2 = 369.90, χ2 /degree of freedom = 2.09, SRMR = .09, RMESA = .07, CFI = .94, TLI = .93). The post-traumatic growth of cured patients with COVID-19 was explained through distress perception, self-disclosure, and deliberate rumination, with the explanatory power being 70.0%. Conclusion: This study suggests preparing a disaster psychology program involving experts who can activate deliberate rumination is necessary. Further, this study may serve as basic data for developing a program to enhance the post-traumatic growth of patients cured from COVID-19.
Traditional manual identification of crop leaf diseases is challenging. Owing to the limitations in manpower and resources, it is challenging to explore crop diseases on a large scale. The emergence of artificial intelligence technologies, particularly the extensive application of deep learning technologies, is expected to overcome these challenges and greatly improve the accuracy and efficiency of crop disease identification. Crop leaf disease identification models have been designed and trained using large-scale training data, enabling them to predict different categories of diseases from unlabeled crop leaves. However, these models, which possess strong feature representation capabilities, require substantial training data, and there is often a shortage of such datasets in practical farming scenarios. To address this issue and improve the feature learning abilities of models, this study proposes a deep transfer learning adaptation strategy. The novel proposed method aims to transfer the weights and parameters from pre-trained models in similar large-scale training datasets, such as ImageNet. ImageNet pre-trained weights are adopted and fine-tuned with the features of crop leaf diseases to improve prediction ability. In this study, we collected 16,060 crop leaf disease images, spanning 12 categories, for training. The experimental results demonstrate that an impressive accuracy of 98% is achieved using the proposed method on the transferred ResNet-50 model, thereby confirming the effectiveness of our transfer learning approach.
The self-report measure is a useful tool for evaluating self-recognized disability and difficulty in daily living activities. Although many studies and clinics used the Neck Pain and Disability Scale (NPDS) for measuring neck pain and functional impairment, there has not been much adaptation of this for use with Korean patients. The purpose of this study was to establish the reliability and validity of NPDS among Korean neck pain patients. Fifty-five subjects (32 males, 23 females) with neck pain enrolled in this study. They completed standardized self-administered questionnaires. The NPDS measures pain intensity; its interference with vocational, emotional, recreational, social, and functional aspects of living; and the presence and extent of associated factors. Reliability was determined by the intraclass correlation coefficient (ICC) and Cronbach's alpha for internal consistency. Validity was examined by correlating the NPDS scores to the Visual Analog Scale (VAS) score. The test-retest reliability of the translated versions of the NPDS was good ICC (2,1)=.86 (95%CI .76~.92). Cronbach's alpha value for NPDS was found to be .93, and this was statistically significant (p<.05). The criterion-related validity coefficient was .79 (p<.Oll. We conclude that the Korean version of NPDS has been shown to be a reliable and valid instrument for the assessment of neck pain. Successful linguistic and cultural translation will admit appropriate cross-cultural comparison for clinical analysis. Therefore, this study can be expected to be used as an adequate evaluation scale for neck pain related studies and treatments.
The purpose of this study was to examine the mediating effect of functional and dysfunctional expressions of anger on the relationship between covert narcissism and dating violence among college students. The study also investigated gender differences with regard to covert narcissism, dating violence, and functional and dysfunctional expressions of anger. To this end, 339 college students (133 males, 206 females) with heterosexual dating experiences in the Busan Metropolitan area were recruited. They responded to the Covert Narcissism Scale, the Revised Conflict Tactics Scale 2, and the Korean adaptation of the State-Trait Anger Expression Inventory. The results were as follows. First, covert narcissism, dating violence, and functional and dysfunctional expressions of anger differed significantly by gender. Second, there were statistically significant correlations between covert narcissism, dating violence, and functional and dysfunctional expressions of anger. Third, multiple regression analysis showed that dysfunctional expressions of anger for males were positively correlated with dating violence while functional expressions of anger were negatively correlated. For females, dysfunctional expressions of anger were positively correlated with dating violence while functional expressions of anger were not significantly correlated. Fourth, dysfunctional expressions of anger completely mediated the relationship between covert narcissism and dating violence for both males and females, but functional expressions of anger had no mediating effect. These results suggest that dysfunctional anger expression control program should be developed for students which may help those with covert narcissistic tendencies to functionally express their anger. Future research should analyze dating violence prevention and counseling programs.
This study was done to analyze the trends of research on coping in Korea, to suggest future direction, for research on coping, and ultimately to contribute to an increase in explanation of adaptation. This article reviewed 79 nursing research papers on coping done since 1978 by examining them according to the period of publication or presentation, research design, type of subjects, measurement instruments, research for a degree or not, range of reliability, and association of coping and related variables. The results are as follows : The number of studies on coping increased rapidly from the mid-1980's and decreased slowly from the mid-1990's. The maority of the studies were surveys, comparative studies, or correlational studies. The subects of the 46 studies were healthy people, while those in the remaining studies were patients with a variety of illnesses. Thirth-eight studies on coping were done for master's thesis, three for dissertion, and 38 were not degrees. The Bell and Jalowiec coping scales have not been used since the early 1990's. In contrast, Lazarus and Folkman's W.C.C.L. has been used increasingly since that time. The reliabilities of the coping scale were reported in 37 cases and the Cronbach's alpha coefficients were .71 to .86. All subjects reported using more problem-oriented coping than emotion-oriented coping in short-term or emotion-oriented coping and healthy groups did more long-term coping. It was difficult to describe consistently the relationship between stress and coping according to the type of coping scale or research subjects, but generally moderate relationships were found. This was due to instrumental problems and no consideration of situational context. The subject group who used more short-term coping and less long-term coping reported poorer mental status, and higher scores in burnout and state anxiey than others. That is, the relationship between stress and adaptation increased the power of explanation with intervening the mediating effect of coping. The association of locus of control, mastery, social support, and self-concept with coping showed positive relationships : those of uncertainty and severity in illness with coping showed negativerelationships ; those of state anxiety and depression with short-term coping were positive, and those of self-esteem with long-term coping or problem-oriented coping were negative. There were significant differences in the scores of types of coping according to religion, level of education, and socio-economic status. That is, Presbyterians and Catholics, those with higher education levels and higher socio-economic status used more long-term or problem-oriented coping. On the basis of the above findings the following recommendations are made : 1. There is a need to test the mediating effect of coping variable in order to clarify the concept. 2. Longitudinal studies are needed to determine the patterns of change in coping strategies when stressful events are encountered. 3. It's necessary to develop a reliable and variable measurement tool for coping. 4. There is a need to identify subscales of coping to increase explanation of variance 5. It's necessary to consider personal, situational, and antecedent variables : the characteristics of subject populations, the natures of illness and treatment situations. 6. The power of explanation of studies designed to identify the stress-adaptation process should be increased using the combination model of process-oriented coping and cognitive-structural model.
This study aimed to develop and validate the Classroom Problem Behavior Scale - Elementary School Version (CPBS-E) measure which is unique to classroom problem behavior exhibited by Korean elementary school students. The focus was on developing a universal screening instrument designed to identify and provide intervention to students who are at-risk for severe social-emotional and behavioral problems. Items were initially drawn from the literature, interviews with elementary school teachers, common office discipline referral measures used in U.S. elementary schools, penalty point systems used in Korean schools, 'Green Mileage', and the Inventory of Emotional and Behavioral Traits. The content validity of the initially developed items was assessed by six classroom and subject teachers, which resulted in the development of a preliminary scale consisting of 63 two-dimensional items (i.e., Within Classroom Problem Behavior and Outside of Classroom Problem Behavior), each of which consisted of 3 to 4 factors. The Within Classroom Problem Behavior dimension consisted of 4 subscales (not being prepared for class, class disruption, aggression, and withdrawn) and the Outside of Classroom Problem Behavior dimension consisted of 3 subscales (rule-violation, aggression, and withdrawn). The CPBS-E was pilot tested on a sample of 154 elementary school students, which resulted in reducing the scale to 23 items. Following the scale revision, the CPBS-E was validated on a sample population of 209 elementary school students. The validation results indicated that the two-dimensional CPBS-E scale of classroom problem behavior was a reliable and valid measure. The test-retest reliability was stable at above .80 in most of the subscales. The CPBS-E measure demonstrated high internal consistency of .76-.94. In examining the criterion validity, the scale's correlation with the Teacher Observation of Classroom Adaptation-Checklist (TOCA-C) was high and the aggression and withdrawn subscales of the CPBS-E demonstrated high correlations with externalization and internalization, respectively, of the Child Behavior Checklist - Teacher Report Form CBCL-TRF). In addition, the factor structure of the CPBS-E scale was examined using the structural equation model and found to be acceptable. The results are discussed in relation to implications, contributions to the field, and limitations.
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