• 생약학회지
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• 제33권2호통권129호
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• pp.144-150
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• 2002

The methanol extracts of 132 herbal medicines were screened for the inhibitory activity against heparinase enzyme from Flavobacterium heparinum. Eleven medicinal plants, Amomum xanthiodides, Agrimonia pilosa, Paeonia lactiflora, Rubia cordifolia, Sanguisorba officinalis, Torrega grandis, Morus alba, Gleditsia sinensis, Crataegus pinnatifida, Cornus officinalis, Paeonia japonica showed potent inhibition on heparinase activity. The active substituents of those herbal medicine could be extracted into butanol fraction and the inhibitory compounds of Morus alba are now isolating.

• 한국약용작물학회지
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• 제13권5호
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• pp.219-226
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• 2005

Sanguisorbae radix (SR) from Sanguisorba officinalis L. (Losaceae) is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of SR on amyloid ${\beta}$ Protein(25-35) $(A{\beta}\;(25-35))$, a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. SR, over a concentration range of $10-50\;{\mu}g/ml$, inhibited the $A{\beta}$ (25-35) $(10\;{\mu}M)-induced$ neuronal cell death, as assessed by a 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. Pretreatment of SR $(50\;{\mu}g/ml)$ inhibited $10\;{\mu}M\;A{\beta}$ (25-35)-induced} elevation of cytosolic calcium concentration $([Ca^{2+}]c)$, which was measured by a fluorescent dye, fluo-4 AM. SR $(10\;and\;50\;{\mu}g/ml)$ inhibited glutamate release into medium induced by $10\;{\mu}M\;A{\beta}(25-35)$, which was measured by HPLC, and generation of reactive oxygen species. These results suggest that SR prevents $A{\beta}$ (25-35)-induced neuronal cell damage in vitro.

• The Korean Journal of Physiology and Pharmacology
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• 제18권6호
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• pp.469-474
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• 2014

DWP208 is a sodium succinate form of ZYM-201 which is a triterpenoid glycoside isolated from Sanguisorba officinalis, a medicinal plant prescribed for various diseases, such as duodenal ulcers and bleeding in East Asian counties. We demonstrated that this compound is able to normalize the altered lipid metabolism induced by hyperglycemia and a high fat diet. In this study, we determined whether hyperlipidemic conditions induced with chronically treated alcohol can also be restored by DWP208. Similar to our previous results, orally administered DWP208 (1 to 10 mg/kg) also ameliorated the hyperlipidemia that was induced by alcohol. This compound reversed the alcohol-induced hyperlipidemia including (i) up-regulated hyperlipidemic parameters such as low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), atherosclerotic index (AI), triglyceride, and total cholesterol, and (ii) down-regulated hyperlipidemic parameters such as absolute body weight, superoxide dismutase (SOD) activity, and high-density lipoprotein (HDL) in serum and liver. According to our data, the ameliorative activity of DWP208 is due to its indirect anti-oxidative activity as a result of which lipid peroxide and hydroxyl radical levels were reduced and the activity of SOD was enhanced. Therefore, our data strongly suggest that DWP208 can be used as a remedy against alcohol-induced hyperlipidemia.

• 대한본초학회지
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• 제38권5호
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• pp.21-29
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• 2023

Objects : This study aimed to identify the origin species of Ulmi Cortex and Ulmi Semen Pasta. Methods : I searched for different types of 'Yu(楡)' from Zhong Xiu Zheng Huo Jing Shi Zheng Lei Bei Yong Ben Cao (重修政和經史證類備用本草) of the Song Dynasty, then compared the descriptions of Yu with real life species. Items that were inconsistent were sequentially deleted to finally find the original species that satisfies all conditions. Results : There were five types of Yu : Jiyu(地楡), Jayu(刺楡), Langyu(郞楡), Goyu(姑楡), and Baekyu(白楡). Jiyu was Sanguisorba officinalis or Sanguisorba officinalis var. longifolia which was a herb. Jayu was Hemiptelea davidii (Hance) Planch. which had thorns on its branches. Ulmus laciniata had leaves that split into three branches. Langyu was Ulmus parvifolia which beared fruit in the fall. For these reasons, the four items above were ruled out. The fruit of Goyu was Ulmi Semen Pasta, which was the largest among fruits and was therefore Ulmus macrocarpa. The fruit of Baekyu was Yuhyup(楡莢) and the ancient coin Yuhyupjeon(楡莢錢) was resembled Yuhyup. The normal pulse of the lung resembled the appearance of falling Yuhyup. The peel of Baekyu was Yubaekpi(楡白皮), and when it was removed from coarse bark, contained an abundance of white sap; the place where this sap overflowed on the stem bark was white, therefore Baekyu was identified as Ulmus pumila. Conclusions : From the results above, the origin species of Ulmi cortex was Ulmus pumila and the origin species of Ulmi Semen Pasta was Ulmus macrocarpa.

• 생명과학회지
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• 제33권5호
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• pp.406-413
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• 2023

본 연구에서는 기능성 화장품 소재로써 오이풀 뿌리 추출물의 다양한 생리활성 및 항염증 활성을 연구하였다. 오이풀 뿌리 추출물의 항산화능을 알아보기 위해 전자공여능을 측정한 결과 농도가 증가함에 따라 활성이 증가하였으며 1,000 ㎍/ml의 농도에서 93.8%의 우수한 항산화능을 나타내었다. 또한, ABTS⁺radical scavenging activity 측정을 통해 알아본 오이풀 뿌리 추출물의 항산화력은 50 ㎍/ml 이상의 농도에서부터 99% 이상을 나타내었다. 미백활성 검증을 위해 tyrosinase 저해활성 측정을 시행하였으며 최고 농도인 1,000 ㎍/ml에서 37.7%의 저해율을 나타내었다. 오이풀 뿌리 추출물의 주름개선 활성을 알아보기 위해 elastase 및 collagenase 저해활성을 측정한 결과 농도 의존적으로 저해율이 증가하였으며 각각 1,000 ㎍/ml의 농도에서 84.9%, 90.3%의 저해율을 나타내었다. MTT assay에 따른 Raw 264.7 cell의 생존율을 확인한 결과 100 ㎍/ml 농도 이하에서 80% 이상의 세포 생존율을 보여 이하의 세포 관련 실험에서는 100 ㎍/ml 이하의 농도에서 세포 실험을 시행하였다. 오이풀 뿌리 추출물의 항염증 활성을 알아보기 위해 NO assay를 측정한 결과 500 ㎍/ml의 농도에서 50.8%의 저해율을 나타내었으며 오이풀 뿌리 추출물이 염증발현 억제에 뛰어난 효능이 있음을 확인할 수 있었다. Raw 264.7 cell에 오이풀 뿌리 추출물을 처리하여 단백질 발현저해를 확인한 결과 모든 인자에서 단백질 발현이 농도 의존적으로 저해됨을 확인할 수 있었다. 따라서 오이풀 뿌리가 항산화, 미백 및 주름개선의 생리활성과 항염 활성이 있는 기능성 화장품 소재로써 활용 가능이 적합하다고 판단된다.

• 한국환경복원기술학회지
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• 제9권6호
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• pp.52-62
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• 2006

The flora of vascular plants around Chunji and Sochunji in Mt. Baekdu was listed 120 taxa; 31 families, 81 genera, 97 species, 1 subspecies, 21 varieties and 1 form. About endangered wild plants, 2 taxa (Paeonia obovata, Rhododendron aureum) are in class II. 12 taxa were listed as rare and endangered plants; Disporum ovale, Lilium davuricum, Saxifraga punctata, Rosa marretii, Cnidium tachiroei, Bupleurum euphorbioides, Halenia corniculata, Gentiana jamesii, Pedicularis verticillata, etc. 10 taxa were listed as Korean endemic plants; Lilium amabile, Aconitum pseudolaeve var. erectum, Oxytropis anertii for. alba, Valeriana officinalis var. latifolia, Cardamine resedifolia var. morii, Homopteryx nakaiana, Saussurea alpicola, Ligularia jamesii, etc. Specific plants by floral region were total 47 taxa; 3 taxa (Paeonia obovata, Rhododendron aureum, Pedicularis verticillata) in class V, 15 taxa (Lycopodium annotinum, Alnus maximowiczii, Saxifraga punctata, Petasites saxatile, etc.) in class IV, 5 taxa (Spiraea salicifolia, Sanguisorba hakusanensis, Acer ukurunduense, Campanula glomerata var. dahurica, Cacalia adenostyloides) in class III, 18 taxa (Lycopodium chinense, Disporum ovale, Ceologlossum viride var. bracteatum, Betula ermani, Aruncus dioicus var. kamtschaticus, Filipendula multijuga, Geranium eriostemon, Cnidium tachiroei, Ligularia stenocephala, etc.) in class II, 6 taxa (Pinus koraiensis, Cimicifuga simplex, Sanguisorba tenuifolia var. alba, Senecio nemorensis, Cacalia hastata subsp. orientalis, Cirsium pendulum) in class I. Peculiar flora around Chunji is thought to be made by the environmental factors such as wind, temperature and rainfall that influence the plants' growth.

• Preventive Nutrition and Food Science
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• 제10권1호
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• pp.81-87
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• 2005

The objective of this study was to determine the radical scavenging activity, total phenolic content, antimicrobial activity, minimum inhibitory concentration (MIC) of ethanol extracts of 32 medical plant species that have been commonly used in medicinal plants. Total phenolic index of T. chebula exhibited the highest value (498.01㎎/g), followed by R. coreanus miquel (400.33㎎/g), Sanguisorba officinalis (368.25㎎/g), P. thumbergiana (259.74㎎/g) and Eugenia aromaticum (229.38㎎/g). Radical scavenging activity for the DPPH radical was highest in T. chebula (40.91%, p<0.01), followed by C. sappan (36.50%), S. officinalis (32.92%), R. coreanus miquel (26.54%) and P. thumbergiana (24.50%). The extracts from T. chebula, R. coreanus muquel, C. sappan, E. aromaticum, S. officinalis and C. japonica possessed outstanding antimicrobial activity against Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Lactobacillus plantarum. MIC was determined on those extracts that showed high efficacy against the test organisms. The most potent MIC values were seen for T. chebula extract against P. aeruginosa, S. aurusa, E. coli, B. subtilis, L. plantarum and S. Typhimurium at 7.8, 7.8, 15.6, 7.8, 125 and 31.2㎍/mL, respectivley. Furthermore, the total phenolic content and radical scavenging activity were very closely correlated for all samples (r=0.78). The coefficient correlations between total phenolic index and antimicrobial activity were 0.91 (E. coli), 0.91 (B. subtillis), 0.79 (P. aeruginosa), 0.79 (S. Typhimurium) and 0.70 (L. plantarum).

• Biomolecules & Therapeutics
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• 제11권3호
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• pp.196-199
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• 2003

The methanol extracts from 60 plant extracts were prepared and evaluated for their antioxidative activity. Arctium lappa, Diospyros kaki, Eugenia caryophyllata, Melia azedarach and Forsythia suspensa showed the inhibitory activity against lipid peroxidation. Caesalpinia sappan, Crataegus pinnatifida, Eugenia caryophyllata, Gleditsia japonica, Osmunda japonica, Rhus javanica and Sanguisorba officinalis showed the highest inhibitory activity against DPPH radical formation. In particular, Eugenia caryophyllata demonstrated strong inhibitory activity on the lipid peroxidation and free radicals. The results suggested that selected plant extracts have a potential as natural antioxidant.

• Natural Product Sciences
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• 제9권2호
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• pp.87-90
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• 2003

The purpose of this study was to find the effect of 36 traditional medicinal plant species on hepatoprotective activity screening. For this study, carbon tetrachloride $(CCl_{4})$ intoxificated rats were used. Test extracts were made with the traditional medicinal plants refluxed in 95% MeOH and orally administered to the rats. Sixteen species, such as Mentha arvensis, Sophora japonica, Benincasa hispida, Lonicera japonica (Lonicerae Flos), Agaricus blazei, Epimedium koreanum, Aralia continentalis, Lithospermum erythrorhizon, Cimicifuga foetida, Gastrodia elata, Sanguisorba officinalis, Cephalonoplos segetum, Bupleurum falcatum, Alisma plantago-aquatica var. orientale, Lonicera japonica (Lonicerae Folium) and Sinomenium acutum showed protective effect against increased serum alanine aminotransferase (ALT) and/or serum asparteate aminotransferase (AST) activities.

• 한국식품영양학회지
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• 제3권2호
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• pp.177-184
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• 1990

General components, amino acids and minerals contained in T kind of edible wild grasses were estimated. Samples were collected from the field of Kapyong area, Kyung-ki, Korea. Results of the estimation were summarized as follows ; 1) A relatively large amounts of protein and fat were contained in the edible parts of each sample. 2) The most abundant amino acids in the edible parts of each sample were glutamic acid (26.6%), serine(18.7%), valise(22.4%), respectively. 3) A high level of calcium and iron was found in the edible parts of each samples. Especially calcium was abundant in the leaves of Rumex coreanus Nakai and iron was abundant in the Commelina communis L. Both copper and zinc were abundant in the Sanguisorba officinalis L.