• 제목/요약/키워드: Sample pre-treatment

검색결과 223건 처리시간 0.031초

전국 간호전문대학 남학생의 제특성에 관한 조사연구 (Characteristics of Male Diploma Nursing Students in Korea)

  • 김혜성
    • 대한간호학회지
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    • 제9권2호
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    • pp.63-72
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    • 1979
  • This study was performed to investigated of characteristics of male diploma nursing students. Data were collected by means of a mailed questionnaire. The sample included 29 respondents from 3 diploma nursing colleges during the period of Nov. 1st-15th, 1978. Major findings included. 1 ) Motive by which the objects of this investigation have chosen the science of nursing. Twenty-one (72.4%) responded, “As nursing occupation is a public welfare work, ”the highest rate, eighteen (62.3%) chose on advices of their parents and acquaintances. Seventeen (58.6%) reflected as a means of life with an occupation in hope of employment abroad. 2) Appreciation of nursing occupation. Twenty-two (75.9%) of opinions that the nursing job is called for by society was pre-dominent. While eighteen (62.1%) replied, “It is the job fit for the male sex, too.”“It is admitted as specialized occupation.”, or“It needs various human relation.”3) Degree of satisfaction wilt the science of nursing. Fifteen (51.7%) responded neither satisfied nor dissatisfied, while eight (27.6%) indicated as “satisfied”and four (13.8%) as“dissatisfied.”4) Degree of satisfaction with the faculty. Sixteen (55.2%) replied, “common, ”the highest, while ten (34.5%) indicated as“dissatisfied, ”two (6.9%) as“satisfied.”The reason for dissatisfaction with the faculty; The responses regarding dissatisfaction was twenty-three (79.3%) as insufficiency of the faculty. Thirteen (44.8%) indicated“the lack of personal cultivation of the faculty.”, And eleven (37.9%) indicated as“the quantitive shortage of the faculty, ”or“the vagueness of learning estimation.”5) Degree of satisfaction with the clinical, training. Eight (27.6%) responded as“common, ”or “dissatisfied, ”while seven (24.1%) indicated as“satisfied.”Reason for dissatisfaction with the training ; Twenty (69.0%) indicated“deficiency of personal treatment to the students of the men of business in the hospital”with respect to the reason, eighteen (62.1%) was indicated as gap between theory and practice, while eleven (37.9%) indicated“insufficiency of the equipment and materials of the hospital.”6) Interest in employment after graduation. Twenty-five (86.2%) indicated“going abroad”while fifteen (51.7%) indicated “education of nursing, ”which were the highest responses. Thirteen (44.8%) chose“Community Health Nursing (Health Center, Industrial Health).”7) Interest an employment during clinical nursing. Sixteen (55.2%) was interested in an operating room or the department of anesthesia, while fifteen (51.7%) was indicated “psychiatry, ”Eight (21.6%) chose a intensive care unit or a emergency room.

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결혼이주여성의 구강보건교육 프로그램 적용 후 구강상태의 변화 (The Change of the Oral Health Status after Applying the Dental Health Education Program for International Marriage Migrant Women)

  • 최미숙
    • 한국산학기술학회논문지
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    • 제14권1호
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    • pp.206-213
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    • 2013
  • 본 연구는 구강건강 증진을 위한 구강건강신념의 형성을 위해서 결혼이주여성들은 대상으로 구강보건교육 프로그램을 개발 실시하여 구강보건교육 실시 전과 실시 후가 구강건강 지식 및 태도에 어떤 효과가 있는지 진단해보고 실제적으로 구강상태를 조사하여 구강건강증진 어떤 변화가 있는지 알아보고자한다. 다문화가족지원센터에서 2012년 3월 26일에서 2012년 6월 30일까지 연구대상자는 실험집단, 통제집단 각각 51명으로 구성되었고, 구강지식과 인식을 사전-사후 조사했으며 인식에 변화가 있어 치과를 방문한 사람을 추후 조사하여 구강상태를 조사하여 대응표본t검증, 반복측정 변량분석을 실시하였다. 그 결과 구강보건 인식에서는 구강상식, 치주질환, 치아우식증, 잇솔질 영역에서 향상 나타났고 구강보건지식에서는 치주질환, 치아우식증, 잇솔질, 불소, 구취영역에서 구강보건인식에서 향상 나타났다. 우식경험영구지수(DMFT index)변화 즉 우식경험 미처치 치아수 (DT index)가 줄어들고 우식경험 처치치아수 (FT index) 늘어났다. 이상의 결과와 같이 본 연구에서 개발한 국제결혼이주 여성을 위한 구강보건 교육 프로그램은 결혼이주여성들의 구강보건인식. 지식 등의 변화를 가져왔으며 지식의 변화는 행동의 영향을 주어 치주상태, 우식경험 영구치지수의 변화도 있었다. 이는 결혼이주여성의 구강보건을 위한 교육프로그램의 중요성을 보여주고 있으며, 추후 구강보건교육 프로그램 개발을 위한 정보를 제공하여 다양한 측면에서 구강보건교육 프로그램이 개발되어 예방과 치료를 병행할 수 있게 되어야할 것이다.

미생물활성화제를 이용한 유류오염토양 복원에 관한 연구 (A Study on the Remediation using Microbial Activator from Oil-Contaminated Soil)

  • 이채영;정찬교;김종문
    • 유기물자원화
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    • 제19권2호
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    • pp.41-48
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    • 2011
  • 본 연구에서는 미생물활성화제를 토양경작법에 적용하였을 경우 토양을 복원함에 있어 타 공법에 비해 장시간 걸리는 단점을 최소화하고, 빠른 시일 내에 친환경적으로 복원이 가능한지에 대한 타당성 조사와 더불어 석유계총탄화수소(TPH)의 저감 능력을 확인하였다. Pre-test의 개념으로 미생물활성화제의 성능과 분해 효율을 lab-test를 통해 확인하였으며, 유류오염 토양의 지표인 석유계총탄화수소(TPH)의 효과를 확인하였다. 석유계총탄화수소(TPH)의 처리 효과를 확인한 결과, 20일 정도까지는 자연분해와 미생물활성화제의 차이가 미미하게 발생하였으나, 20일 경과 후에는 처리 효과가 대조군에 비해 높게 나타나는 것을 확인할 수 있었다. 또한, 각층에 따른 제거율을 살펴본 결과, 상층 85.8 %, 중층 84.4 %의 제거율을 나타냈으나, 하층에서는 66.10 % 제거율을 나타냈다. 대조군에서 자연적으로 줄어드는 석유계총탄화수소(TPH)의 저감율이 평균 71.1 %임을 근거로 봤을 때 미생물활성화제가 하층까지 충분하게 전달되지 않은 상태로 볼 수 있었으며, 이는 토양 더미의 문제로 판단된다. 현장 실험에서는 토양 더미가 1 m 로 진행되었기 때문에 더미 높이를 0.6 m 이하로 낮추게 되면 석유계총탄화수소(TPH)의 처리효율은 더 높아질 수 있을 것으로 사료된다.

Multi-dimensional GC-MS를 이용한 항공터빈유의 첨가제 분석 (Determination of Additives Content in Aviation Turbine Fuel Using Multi-dimensional GC-MS)

  • 연주민;장윤미;임의순;김성룡;강용
    • 한국응용과학기술학회지
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    • 제35권4호
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    • pp.1260-1268
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    • 2018
  • 항공터빈유는 등유 기반의 석유제품에 산화 방지제(Antioxidant), 빙결 방지제(Fuel system icing inhibitor, FSII), 전기전도도 향상제(Electrical conductivity improver) 등의 첨가제를 첨가하여 항공기 연료로서 필요한 성능 향상 및 보관이나 이송 등에 관한 특정한 능력을 부여시키고 있다. 이들 첨가제는 항공터빈유의 품질에 이상이 발생하거나 다른 석유제품과 구별하기 위하여 그 첨가량을 정성 및 정량적으로 분석할 수 있어야 한다. 항공터빈유는 수많은 탄화수소 화합물로 구성된 복잡한 화합물이기 때문에 미량으로 첨가된 산화 방지제와 빙결 방지제를 분석하기 위하여 Multi-dimensional GC-MS (MDGC-MS)의 Deans switching 기술을 적용하였다. 2.5 - 20 mg/L 농도 범위의 산화 방지제와 빙결 방지제를 MDGC-MS로 정량 및 정성적으로 분석할 수 있었으며, 검출 한계는 1-dimensional GC-MS의 분석 결과와 비교하여 약 2배 정도 낮았다. 본 연구에서 개발된 시험 방법은 기존의 GC-MS보다 첨가제 피크의 분리능이 더 우수하였으며, 시료의 전처리가 필요없이 두 가지 첨가제를 동시에 분석할 수 있었다.

농촌(農村) 주민(住民)들의 의료필요도(醫療必要度)에 관(關)한 연구(硏究) (A Study Concerning Health Needs in Rural Korea)

  • 이성관;김두희;정종학;정극수;박상빈;최정헌;홍순호;라진훈
    • Journal of Preventive Medicine and Public Health
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    • 제7권1호
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    • pp.29-94
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    • 1974
  • Today most developed countries provide modern medical care for most of the population. The rural area is the more neglected area in the medical and health field. In public health, the philosophy is that medical care for in maintenance of health is a basic right of man; it should not be discriminated against racial, environmental or financial situations. The deficiency of the medical care system, cultural bias, economic development, and ignorance of the residents about health care brought about the shortage of medical personnel and facilities on the rural areas. Moreover, medical students and physicians have been taught less about rural health care than about urban health care. Medical care, therefore, is insufficient in terms of health care personnel/and facilities in rural areas. Under such a situation, there is growing concern about the health problems among the rural population. The findings presented in this report are useful measures of the major health problems and even more important, as a guide to planning for improved medical care systems. It is hoped that findings from this study will be useful to those responsible for improving the delivery of health service for the rural population. Objectives: -to determine the health status of the residents in the rural areas. -to assess the rural population's needs in terms of health and medical care. -to make recommendations concerning improvement in the delivery of health and medical care for the rural population. Procedures: For the sampling design, the ideal would be to sample according to the proportion of the composition age-groups. As the health problems would be different by group, the sample was divided into 10 different age-groups. If the sample were allocated by proportion of composition of each age group, some age groups would be too small to estimate the health problem. The sample size of each age-group population was 100 people/age-groups. Personal interviews were conducted by specially trained medical students. The interviews dealt at length with current health status, medical care problems, utilization of medical services, medical cost paid for medical care and attitudes toward health. In addition, more information was gained from the public health field, including environmental sanitation, maternal and child health, family planning, tuberculosis control, and dental health. The sample Sample size was one fourth of total population: 1,438 The aged 10-14 years showed the largest number of 254 and the aged under one year was the smallest number of 81. Participation in examination Examination sessions usually were held in the morning every Tuesday, Wenesday, and Thursday for 3 hours at each session at the Namchun Health station. In general, the rate of participation in medical examination was low especially in ages between 10-19 years old. The highest rate of participation among are groups was the under one year age-group by 100 percent. The lowest use rate as low as 3% of those in the age-groups 10-19 years who are attending junior and senior high school in Taegu city so the time was not convenient for them to recieve examinations. Among the over 20 years old group, the rate of participation of female was higher than that of males. The results are as follows: A. Publie health problems Population: The number of pre-school age group who required child health was 724, among them infants numbered 96. Number of eligible women aged 15-44 years was 1,279, and women with husband who need maternal health numbered 700. The age-group of 65 years or older was 201 needed more health care and 65 of them had disabilities. (Table 2). Environmental sanitation: Seventy-nine percent of the residents relied upon well water as a primary source of dringking water. Ninety-three percent of the drinking water supply was rated as unfited quality for drinking. More than 90% of latrines were unhygienic, in structure design and sanitation (Table 15). Maternal and child health: Maternal health Average number of pregnancies of eligible women was 4 times. There was almost no pre- and post-natal care. Pregnancy wastage Still births was 33 per 1,000 live births. Spontaneous abortion was 156 per 1,000 live births. Induced abortion was 137 per 1,000 live births. Delivery condition More than 90 percent of deliveries were conducted at home. Attendants at last delivery were laymen by 76% and delivery without attendants was 14%. The rate of non-sterilized scissors as an instrument used to cut the umbilical cord was as high as 54% and of sickles was 14%. The rate of difficult delivery counted for 3%. Maternal death rate estimates about 35 per 10,000 live births. Child health Consultation rate for child health was almost non existant. In general, vaccination rate of children was low; vaccination rates for children aged 0-5 years with BCG and small pox were 34 and 28 percent respectively. The rate of vaccination with DPT and Polio were 23 and 25% respectively but the rate of the complete three injections were as low as 5 and 3% respectively. The number of dead children was 280 per 1,000 living children. Infants death rate was 45 per 1,000 live births (Table 16), Family planning: Approval rate of married women for family planning was as high as 86%. The rate of experiences of contraception in the past was 51%. The current rate of contraception was 37%. Willingness to use contraception in the future was as high as 86% (Table 17). Tuberculosis control: Number of registration patients at the health center currently was 25. The number indicates one eighth of estimate number of tuberculosis in the area. Number of discharged cases in the past accounted for 79 which showed 50% of active cases when discharged time. Rate of complete treatment among reasons of discharge in the past as low as 28%. There needs to be a follow up observation of the discharged cases (Table 18). Dental problems: More than 50% of the total population have at least one or more dental problems. (Table 19) B. Medical care problems Incidence rate: 1. In one month Incidence rate of medical care problems during one month was 19.6 percent. Among these health problems which required rest at home were 11.8 percent. The estimated number of patients in the total population is 1,206. The health problems reported most frequently in interviews during one month are: GI trouble, respiratory disease, neuralgia, skin disease, and communicable disease-in that order, The rate of health problems by age groups was highest in the 1-4 age group and in the 60 years or over age group, the lowest rate was the 10-14 year age group. In general, 0-29 year age group except the 1-4 year age group was low incidence rate. After 30 years old the rate of health problems increases gradually with aging. Eighty-three percent of health problems that occured during one month were solved by primary medical care procedures. Seventeen percent of health problems needed secondary care. Days rested at home because of illness during one month were 0.7 days per interviewee and 8days per patient and it accounts for 2,161 days for the total productive population in the area. (Table 20) 2. In a year The incidence rate of medical care problems during a year was 74.8%, among them health problems which required rest at home was 37 percent. Estimated number of patients in the total population during a year was 4,600. The health problems that occured most frequently among the interviewees during a year were: Cold (30%), GI trouble (18), respiratory disease (11), anemia (10), diarrhea (10), neuralgia (10), parasite disease (9), ENT (7), skin (7), headache (7), trauma (4), communicable disease (3), and circulatory disease (3) -in that order. The rate of health problems by age groups was highest in the infants group, thereafter the rate decreased gradually until the age 15-19 year age group which showed the lowest, and then the rate increased gradually with aging. Eighty-seven percent of health problems during a year were solved by primary medical care. Thirteen percent of them needed secondary medical care procedures. Days rested at home because of illness during a year were 16 days per interviewee and 44 days per patient and it accounted for 57,335 days lost among productive age group in the area (Table 21). Among those given medical examination, the conditions observed most frequently were respiratory disease, GI trouble, parasite disease, neuralgia, skin disease, trauma, tuberculosis, anemia, chronic obstructive lung disease, eye disorders-in that order (Table 22). The main health problems required secondary medical care are as fellows: (previous page). Utilization of medical care (treatment) The rate of treatment by various medical facilities for all health problems during one month was 73 percent. The rate of receiving of medical care of those who have health problems which required rest at home was 52% while the rate of those who have health problems which did not required rest was 61 percent (Table 23). The rate of receiving of medical care for all health problems during a year was 67 percent. The rate of receiving of medical care of those who have health problems which required rest at home was 82 percent while the rate of those who have health problems which did not required rest was as low as 53 percent (Table 24). Types of medical facilitied used were as follows: Hospital and clinics: 32-35% Herb clinics: 9-10% Drugstore: 53-58% Hospitalization Rate of hospitalization was 1.7% and the estimate number of hospitalizations among the total population during a year will be 107 persons (Table 25). Medical cost: Average medical cost per person during one month and a year were 171 and 2,800 won respectively. Average medical cost per patient during one month and a year were 1,109 and 3,740 won respectively. Average cost per household during a year was 15,800 won (Table 26, 27). Solution measures for health and medical care problems in rural area: A. Health problems which could be solved by paramedical workers such as nurses, midwives and aid nurses etc. are as follows: 1. Improvement of environmental sanitation 2. MCH except medical care problems 3. Family planning except surgical intervention 4. Tuberculosis control except diagnosis and prescription 5. Dental care except operational intervention 6. Health education for residents for improvement of utilization of medical facilities and early diagnosis etc. B. Medical care problems 1. Eighty-five percent of health problems could be solved by primary care procedures by general practitioners. 2. Fifteen percent of health problems need secondary medical procedures by a specialist. C. Medical cost Concidering the economic situation in rural area the amount of 2,062 won per residents during a year will be burdensome, so financial assistance is needed gorvernment to solve health and medical care problems for rural people.

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Fate of Heavy Metals in Activated Sludge: Sorption of Heavy Metal ions by Nocardia amarae

  • Kim, Dong-wook
    • 한국환경과학회:학술대회논문집
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    • 한국환경과학회 1998년도 가을 학술발표회 프로그램
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    • pp.2-4
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    • 1998
  • Proliferation of Nocardia amarae cells in activated sludge has often been associated with the generation of nuisance foams. Despite intense research activities in recent years to examine the causes and control of Nocardia foaming in activated sludge, the foaming continued to persist throughout the activated sludge treatment plants in United States. In addition to causing various operational problems to treatment processes, the presence of Nocardia may have secondary effects on the fate of heavy metals that are not well known. For example, for treatment plants facing more stringent metal removal requirements, potential metal removal by Nocardia cells in foaming activated sludge would be a welcome secondary effect. In contrast, with new viosolid disposal regulations in place (Code o( Federal Regulation No. 503), higher concentration of metals in biosolids from foaming activated sludge could create management problems. The goal of this research was to investigate the metal sorption property of Nocardia amarae cells grown in batch reactors and in chemostat reactors. Specific surface area and metal sorption characteristics of N. amarae cells harvested at various growth stages were compared. Three metals examined in this study were copper, cadmium and nickel. Nocardia amarae strain (SRWTP isolate) used in this study was obtained from the University of California at Berkeley. The pure culture was grown in 4L batch reactor containing mineral salt medium with sodium acetate as the sole carbon source. In order to quantify the sorption of heavy metal ions to N amarae cell surfaces, cells from the batch reactor were harvested, washed, and suspended in 30mL centrifuge tubes. Metal sorption studies were conducted at pH 7.0 and ionlc strength of 10-2M. The sorption Isotherm showed that the cells harvested from the stationary and endogenous growth phase exhibited significantly higher metal sorption capacity than the cells from the exponential phase. The sequence of preferential uptake of metals by N. amarae cells was Cu>Cd>Ni. The specific surFace area of Nocardia cells was determined by a dye adsorption method. N.amarae cells growing at ewponential phase had significantly less specific surface area than that of stationary phase, indicating that the lower metal sorption capacity of Nocardia cells growing at exponential phase may be due to the lower specific surface area. The growth conditions of Nocardia cells in continuous culture affect their cell surface properties, thereby governing the adsorption capacity of heavy metal. The comparison of dye sorption isotherms for Nocardia cells growing at various growth rates revealed that the cell surface area increased with increasing sludge age, indicating that the cell surface area is highly dependent on the steady-state growth rate. The highest specific surface area of 199m21g was obtained from N.amarae cell harvested at 0.33 day-1 of growth rate. This result suggests that growth condition not only alters the structure of Nocardia cell wall but also affects the surface area, thus yielding more binding sites of metal removal. After reaching the steady-state condition at dilution rate, metal adsorption isotherms were used to determine the equilibrium distributions of metals between aqueous and Nocardia cell surfaces. The metal sorption capacity of Nocardia biomass harvested from 0.33 day-1 of growth rate was significantly higher than that of cells harvested from 0.5- and 1-day-1 operation, indicatng that N.amarae cells with a lower growth rate have higher sorpion capacity. This result was in close agreement with the trend observed from the batch study. To evaluate the effect of Nocardia cells on the metal binding capacity of activated sludge, specific surface area and metal sorption capacity of the mixture of Nocardia pure cultures and activated sludge biomass were determined by a series of batch experiments. The higher levels of Nocardia cells in the Nocardia-activated sludge samples resulted in the higher specific surface area, explaining the higher metal sorption sites by the mixed luquor samples containing greater amounts on Nocardia cells. The effect of Nocardia cells on the metal sorption capacity of activated sludge was evaluated by spiking an activated sludge sample with various amounts of pre culture Nocardia cells. The results of the Langmuir isotherm model fitted to the metal sorption by various mixtures of Nocardia and activated sludge indicated that the mixture containing higher Nocardia levels had higher metal adsorption capacity than the mixture containing lower Nocardia levels. At Nocardia levels above 100mg/g VSS, the metal sorption capacity of activate sludge increased proportionally with the amount of Noeardia cells present in the mixed liquor, indicating that the presence of Nocardia may increase the viosorption capacity of activated sludge.

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교정치료에 따른 사춘기 성장 아동의 상기도 폭경과 안면 성장 변화와의 관계 연구 (The relationship between upper airway width and facial growth changes in orthodontic treatment of growing children)

  • 김윤지;복규석;이규홍;황용인;박양호
    • 대한치과교정학회지
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    • 제39권3호
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    • pp.168-176
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    • 2009
  • 본 연구의 목적은 아동에서 사춘기 성장 동안 기도의 변화가 안면 성장에 미치는 영향을 평가하는 것이었다. 9 - 11세(평균 10.7세)의 교정환자 36명을 대상으로 하여 초진 시(T1)와 치료 종료 시(T2)에 각각 측모두부방사선사진과 수완부 방사선 사진을 촬영하였다. 측모두부방사선사진상의 기도 폭경을 기준으로 하여 대상을 세 그룹으로 나누었다; 폭경이 좁은 그룹(AW-Narrow: 5.2 - 8.6 mm), 중간 그룹(AW-Medium: 8.9 - 11.5 mm), 넓은 그룹(AW-Wide: 11.7 - 16.0 mm). T1과 T2시기에 각 그룹 간의 안면 계측치의 차이를 비교하였으며 T1 - T2 사이의 계측치의 변화량을 각 그룹별로 비교하여 성장을 평가하였다. 각 그룹의 기도 폭경 증가량은 AW-Narrow, AW-Medium, AW-Wide group에서 각각 4.55 mm, 3.84 mm, 1.94 mm였으며 통계적으로 유의한 차이를 보였다. T1시기의 각 그룹 간의 비교에서 AW-Narrow group에서 다른 그룹에 비해 PFH가 유의하게 작은 값을 나타냈다. T1 - T2 사이의 성장 평가에서는 AW-Narrow group에서 다른 그룹에 비해 PFH/AFH, facial axis, ANS (T1 - T2), and Gn (T1 - T2)의 변화가 유의하게 큰 값을 보였다. 이 시기의 안면 성장 기간 동안 기도 폭경이 좁은 그룹에서 기도 폭경 및 안면 성장의 보상성 변화가 일어난 것으로 생각된다. 바람직하지 않은 안면 성장을 예방하기 위한 외과적 기도 처치를 고려할 때 자발적인 개선 가능성을 고려하여 좀 더 신중한 평가가 필요할 것으로 생각된다.

골격성 III급 부정교합자에서 상악골 전방 이동술 후 코의 변화에 관한 연구 (Nose Changes after Maxillary Advancement Surgery in Skeletal Class III Malocclusion)

  • 강은희;박수병;김종렬
    • 대한치과교정학회지
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    • 제30권5호
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    • pp.657-668
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    • 2000
  • 본 연구는 골격성 III급 부정교합 환자에서 상악골 전방이동 수술 후 야기되는 코의 형태변화에 대해 연구하고 이를 예측할 수 있는 요소를 찾아 교정-악교정 수술 복합 치료계획의 수립과 결과의 예측에 이용하고자 시행되었다. 부산대학교병원 치과진료처 교정과에 내원하여 골격성 III급 부정교합으로 진단되어 술전 교정치료를 받고 Le Fort I 골절단술로 상악골의 전방 이동시키는 동시에 하악골을 후방 이동시킨 남녀 성인 환자 25명을 대상으로 하였다. 이들의 수술전, 후 측모두부규격방사선사진과 정모 및 측모 안면사진을 계측, 분석하여 다음과 같은 결과를 얻었다. 1. 상악골 전방이동에 따른 비첨의 수직적 위치 변화는 상관성이 높았으며 ANt, SNt에서 A point의 전방이동에 의해 ${\beta}_0$는 각각 0.228, 0.257로 나타났다. 2. 상악골 전방이동에 따른 비첨의 수평적 위치변화는 상관성이 높았으며 ANt, SNt, Sn point에서 A point의 전방이류에 의해 ${\beta}_0$는 각각 0.484, 0.431, 0.806으로 나타났다. 3. 상악골의 전방이동에 따른 비익부의 확장정도는A point의 전방이동에 의해 0.002의 ${\beta}_0$를 나타내었으나, 임상적으로 유의한 변화는 없었다. 4. 수술 후 비첨의 수직적 및 전후방적 위치변화를 예측하는 지표로 사용된 ADV, DRI, Prominence of nose, Pre-Op CA 중 ADV 만이 설명변수로서 유의성이 있었다.

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고초균에 의한 생강 발효 추출물의 신경세포 보호 효과 (Neuroprotective effect of fermented ginger extracts by Bacillus subtilis in SH-SY5Y cells)

  • 양희선;김미진;김민아;최정숙
    • Journal of Nutrition and Health
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    • 제54권6호
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    • pp.618-630
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    • 2021
  • 본 연구에서는 생강을 효소처리하여 수용화율을 높인 후 고초균을 이용하여 발효시킨 생강발효물 (FGEs)을 제조하고, 이를 SH-SY5Y에 6-OHDA와 함께 처리하여 세포 보호 효과 및 AChE 저해 활성을 평가하였다. 그 결과, 6-OHDA로 자극된 신경세포에서 FGE를 처리한 모든 실험군에서 세포 생존율이 증가하고 LDH 농도가 감소하였다. 6-OHDA로 유발된 세포자멸사를 억제할 수 있는지 확인하기 위해 핵의 형태학적인 변화 및 caspase-3 활성을 확인하였다. FGE를 처리한 모든 실험군에서 핵의 손상 및 apoptotic body의 감소와 caspase-3 억제 활성을 확인할 수 있었다. 또한 FGE는 AChE의 활성을 유의적으로 감소시켰다. 시료 간의 활성 차이를 비교하였을 때, 생강에 효소 처리 후 고초균으로 발효한 추출물군 (E/BKG와 E/BCG)의 신경세포 보호 활성이 효소 처리하지 않은 발효생강군 (BKG와 BCG) 보다 유의적으로 크게 나타났다. 그러나 발효에 사용된 고초균 2종 간의 활성은 유사하였으며 처리군 간 유의적인 차이를 보이지 않았다. 본 연구 결과로부터 효소처리하여 수용화율을 높여 고초균으로 발효한 FGE는 신경세포 보호 및 AChE 저해 효과를 나타내어 향후 신경질환 연구를 위한 기초자료 제공 및 고부가가치 식품소재 개발에 이용 가능할 것으로 판단된다.

Macrolide계 항생물질 동시분석법 확립 및 모니터링 (Simultaneous Determination and Monitoring of Three Macrolide Antibiotics in Foods by HPLC)

  • 박상욱;이상호;안종훈;정영지;김성철;김지연;금은희;성주현;김상엽;장영미;강찬순
    • 한국식품과학회지
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    • 제42권3호
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    • pp.287-291
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    • 2010
  • 축산물 중 macrolide계 항생물질 3종을 신속분석하기 위하여 효과적인 전처리법을 설정하고 고속액체크로마토그라피(HPLC)를 이용한 동시분석법을 제시하였다. 대상물질은 tilmicosin, tylosin, spiramycin이며, 확립된 분석법을 이용하여 모니터링을 실시하기 위하여 전국 6개 도시인 서울, 부산, 대전, 인천, 대구, 광주에서 수입원산지가 표시된 쇠고기와 돼지고기 및 그 가공품을 수거하여 분석하였다. 전처리법에 있어서 solid phase extraction(SPE)법에 비하여 액상추출법이 더 높은 회수율을 나타내었으며 전처리 단계도 간단하여 대상 항생물질을 분석하기에 적절하였다. 3종의 항생물질 분리를 위한 컬럼은 C18($250\;mm{\times}4.6\;mm$, $5\;{\mu}m$)을 사용하였으며, HPLC 이동상은 0.025M phosphate buffer(pH 2.5) 및 acetonitrile을 이용한 gradient 조건을 설정하였다. UV 검출파장은 spiramycin 경우 232 nm이고, tilmicosin과 tylosin은 288 nm을 이용하였다. 평균회수율은 83.0-90.2%이였으며, 검출한계는 각각 7(spiramycin), 12(tilmiconsin), 12(tylosin) ng/g으로 나타났다. 수입 축산물의 항생물질에 대한 안전성을 검토하기 위하여 국내 유통 중인 수입축산물 및 그 가공품을 대상으로 하여 모니터링을 실시한 결과, 시료는 전국 6개 대도시에서 126건 구입하였으며 모든 시료에서 macrolide계 항생물질이 검출되지 않았다.