• 대한한방부인과학회지
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• 제19권3호
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• pp.95-107
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• 2006

Purpose : Salviae Miltiorrhizae Radix is a herb with an effect on extravasated blood and is widely used in gynecology. This study examine, the effects of Salviae Miltiorrhizae Radix on endometriosis. Methods : Rats with surgically induced endometriosis were administered Salviae Miltiorrhizae Radix for 40 days. The size of the ectopic uterine implants at the serosal wall and the concentration of progesterone, estradiol, TNF-${\alpha}$ and IL-2, 4, 6 and 10 in the blood were examined and compared with the control group. Results : The size of the ectopic uterine implants in the treated group was much smaller than that in the control group. The estradiol concentration was significantly lower in the experimental group than in the control group. The IL-10 level was significantly higher in the experimental group than in the control group. The TNF-${\alpha}$ level was lower in the experimental group than in the control group but the difference was not significant. The progesterone, IL-2, 4, 6 levels were similar in the experimental and control groups. Conclusion : These results indicate that Salviae Miltiorrhizae Radix reduces the size of ectopic uterine implants at the serosal wall and inhibits the growth of ectopic uterine implants. This suggests that Salviae Miltiorrhizae Radix is an effective treatment for endometriosis.

• 대한본초학회지
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• 제30권4호
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• pp.89-94
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• 2015

Objectives : It had been reported that herbal medicines containing polyphenol and flavonoid have been shown to be associated with decreased the cause of aging and variety of disease such as reactive nitrogen species and reactive oxygen species in several recent studies. Salviae miltiorrhizae Radix, origined fromSalvia miltiorrhizaBGE., is one of popular traditional herbal medicines that is commonly used by traditional medicine practitioners. To this date, Salviae miltiorrhizae Radix has more than 2000-year history of mature application. This study was conducted to investigate whether the Salviae miltiorrhizae Radix methanol extract has an inhibitory effect association with oxidation or inflammation.Methods : Cytotoxic activity of Salviae miltiorrhizae Radix methanol extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide solution. Nitric oxide production was measured using griess reagent system. Western blot analysis and measurement for changes of protein expression, nitric oxide synthase and cyclooxygenase-2, also performed.Results : The medicinal plant, Salviae miltiorrhizae Radix, does not impair the cell viability in tested concentration (25-100 μg/ml). SMR showed anti-oxiative effectsin vitroby decreasing electron donating ability, and also showed anti-inflammatory effects suppressing NO and COX-2 expressin in LPS induced RAW264.7 activation. SMR inhibited the generation of intracellular ROS production as dose dependant manner.Conclusions : These results indicate that Salviae miltiorrhizae Radix methanol extract has an anti-inflammatory activities via anti-oxidative effects, and the anti-inflammatory effect was presentedd as dose dependant manner.

• 한방안이비인후피부과학회지
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• 제23권3호
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• pp.165-171
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• 2010

Objective : This clinical research was conducted to test patients with dark circles by external application with Chinensis galla, Lithospermi radix and Salviae miltiorrhizae radix in cosmetics. Methods : We gave prototype cosmetics to 27 patients who volunteered for this clinical research in Kyungwon university from October 14th, 2009 to November 11th. Left inferior palpebra area of patients was treated with Chinensis galla, Lithospermi radix and Salviae miltiorrhizae radix in cosmetics(Experimental group) and right inferior palpebra area of patients was treated with placebo(Control group) for 4weeks. We observed change of darkness of inferior palpebra and subjective satisfaction. Also taking digital image and color meter were used to evaluate the effects of Chinensis galla, Lithospermi radix and Salviae miltiorrhizae radix in cosmetics. Results : Overall evaluation was slightly positive. It is more effective on Right inferior palpebra than on Left inferior palpebra to improve darkness of inferior palpabra in this clinical research. Conclusion : We can guess that cosmetics with Chinensis galla, Lithospermi radix and Salviae miltiorrhizae radix has some therapeutical effects in reducing the symptoms of dark circles.

• 대한약침학회지
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• 제17권1호
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• pp.7-12
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• 2014

Objectives: The objective of this study is to investigate the effects of Salviae Miltiorrhizae Radix hot aqueous extract on nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production and on 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging in macrophages. Methods: Salviae Miltiorrhizae Radix (300 g) was heated at $100^{\circ}C$ with distilled water (2 L) for 4 hours. The extract was filtered and concentrated to 100 mL by using a rotary evaporator, was frozen at $-80^{\circ}C$, and was then freeze-dried by using a freezing-drying system. The RAW 264.7 macrophage was subcultured by using $10-{\mu}g/mL$ lipopolysaccharide (LPS). In order to evaluate cytotoxicity, we performed 3-(4,5-dimrthylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and measured the cell viability. The NO production was measured by using Griess assays, and the $PGE_2$ production was measured by using enzyme immunoassays. The antioxidant activity, the 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging capability, was measured by using the DPPH method. Results: Cell viability with the 1-, 5-, 25-, 125- and $625-{\mu}g/mL$ Salviae Miltiorrhizae Radix hot aqueous extract was not significantly decreased compared to the cell viability without the extract. When 125 and $625{\mu}g/mL$ of Salviae Miltiorrhizae Radix hot aqueous extract were used, nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. When 25, 125, and $625{\mu}g/mL$ of Salviae Miltiorrhizae Radix hot aqueous extract were used, $PGE_2$ production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. The 125- and $625-{\mu}g/mL$ Salviae Miltiorrhizae Radix hot aqueous extracts had high DPPH free-radical scavenging capabilities in RAW 264.7 macrophages. Conclusion: This study indicates that Salviae Miltiorrhizae Radix hot aqueous extract suppresses NO and $PGE_2$ production and improves DPPH free-radical scavenging capability. Thus, it seems that Salviae Miltiorrhizae Radix hot aqueous extract may have an anti-inflammation effect and antioxidant activity.

• 동의생리병리학회지
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• 제18권2호
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• pp.431-435
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• 2004

The purpose of this study was to examine the effects of Salviae Miltiorrhizae Radix on the high fat diet-induced hyperlipidemia in rats. Sixty male Sprague-Dawley rats, around 270g of B.W., were divided into normal, control, sample A and sample B group. Normal and control groups were orally administrated with saline during 4 weeks, sample A and sample B with 252.5mg/kg(rat) and 420.1 mg/kg(rat) of solid extract of Salviae Miltiorrhizae Radix during 4 weeks respectively. Blood samples were collected from each of animals at 4 weeks, and used for the blood chemical analysis. Animals were sacrificed at 4 weeks, and chemical analysis was performed on the collected liver samples. The serum total cholesterol, HDL-cholesterol, LDL-cholesterol levels of sample A group were significantly decreased compared with the control group. On the basis of these of results, it is confirmed that Salviae Miltiorrhizae Radix has the curative effects on the hyperlipidemia in rats induced by high fat diet

• 대한한의학방제학회지
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• 제11권2호
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• pp.173-184
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• 2003

Objectives: This study investigates the effects of Salviae miltiorrhizae Radix, Carthami Flos on Brain ischemia of the rats induced from the Occlusion of Lt. Common Carotid Artery. Methods: I observed effects using light microscopes and examined tissue of parietal lobe and hippocampus and VEGF-immunoreactive cells. Results: A small number of VEGF-immunoreactive cells are observed in the control group. VEGF-immunoreactive cells in Salviae miltiorrhizae Radix-administered group were slightly increased compared with control group. VEGF-immunoreactive cells in Carthami Flos-administered group were significantly increased compared with control group. Neurons of parietal lobe and pyramidal cells of hippocampus in the control group were greatly damaged.(neuronal densitity, form of dendrite and axon) On the other hand, neurons of parietal lobe and pyramidal cells of hippocampus in Salviae miltiorrhizae Radix-administered group were less damaged. Neurons of parietal lobe and pyramidal cells of hippocampus in Carthami Flos-administered group were significantly less damaged compared with control group. Conclusion : Salviae miltiorrhizae Radix, Carthami Flos can effect on stimulating angiogenesis and reducinging the damage of neurons in the rats induced from the Occlusion of Lt. Common Carotid Artery.

• 동아시아식생활학회지
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• 제18권4호
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• pp.618-623
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• 2008

기능성 식품 및 기능성 화장품으로 활용 가능한 소재를 개발하기 위한 목적으로 자외선 차단 효과, 항산화 효과, 미백효과 등을 측정한 결과는 다음과 같다. 1. 자외선 차단 효과는 두충 추출물은 $280{\sim}210\;nm$에서 단삼은 $270{\sim}220\;nm$에서 나타내는 것으로 보아 자외선 차단용 화장품 원료로 사용 시 UV-C영역의 자외선을 흡수하는 목적으로 사용할 수 있을 것이며, 진피는 $400{\sim}210\;nm$에서 천궁은 $380{\sim}220nm$의 거의 자외선 파장의 전 영역에서 흡수를 나타내었다. 2. DPPH 라디칼에 대한 소거능은 천궁 추출물은 79.0%, 두충 추출물은 74.2%, 진피 추출물은 40.4%, 단삼 추출물은 19.4%의 저해율 순서로 천궁 추출물이 가장 높은 전자공여능을 나타내었다. 3. Superoxide dismutase(SOD) 유사활성율은 단삼 추출물은 21.9%, 진피 추출물은 18.3%, 두충 추출물은 11.7% 순으로 나타내었으나, 천궁 추출물은 반응 과정에서 부유물이 생성되어 정확한 값을 측정할 수 없었다. 4. Xanthine oxidase 저해 활성을 측정한 결과, 단삼 추출물은 79.3%, 천궁 추출물은 71.9%, 두충 추출물은 57.5% 순으로 xanthine oxidase 저해 활성도가 매우 높은 것으로 나타났다. 5. Tyrosinase 저해 활성도는 천궁 추출물은 59.5%, 단삼추출물은 22.8%, 진피 추출물은 27.5%, 두충 추출물은 12.4% 순으로 나타내었다. 이상의 결과를 보면 자외선 차단 효과는 진피와 천궁 추출물이 좋은 자외선 차단용 원료로 사용할 수 있을 것이며, 항산화 효과는 네 가지 추출물의 특성을 고려하여 적당히 혼합한 혼합물, 미백 효과는 천궁 추출물을 사용하면 좋은 기능성 식품 및 기능성 화장품 원료로 사용 가능한 것으로 사료된다.

• Natural Product Sciences
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• 제16권4호
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• pp.251-258
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• 2010

In this study, quantitative analysis for the quality evaluation of Salviae Miltiorrhizae Radix using HPLC/UV was developed. For quantitative analysis, six major bioactive compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS $C_{18}$ column ($250{\times}4.6\;mm$, $5\;{\mu}m$) with gradient condition of A (1% formic acid in $H_2O$) and B (acetonitrile : methanol : formic acid = 100 : 75 : 1) as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 280 nm. These methods were fully validated with respect to the linearity, accuracy, precision and recovery. The HPLC/UV method was applied successfully to the quantification of six major compounds in the Salviae Miltiorrhizae Radix. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.46-46
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• 2003

Salviae Miltiorrhizae Radix has been used for treatment of cardiovascular diseases in oriental medicine. To investigate the possible involvement of cardiac ion channel in this effect, we examined electrophysiological effects of the extract of Salviae Miltiorrhizae Radix on action potentials and ionic currents in rat ventricular myocytes. The extracts of Salviae Miltiorrhizae Radix were fractionated into nine fractions, and the effect of each fraction on action potential was tested. The fraction containing monomethyl lithospermic acid-A (LSA-A) induced a significant prolongation of action potential duration (APD). LSA-B which is a major component of Salviae Miltiorrhizae Radix, however, did not cause a significant effect. In voltage clamp experiments, the effects of LSA-A on K currents, Ca currents and Na currents were tested. Neither K currents nor L-type Ca currents were affected by LSA-A. On the contrary, LSA-A significantly slowed down the inactivation kinetics of the Na current with no effect on the fast component of the inactivation process. The amplitude of the peak current and the voltage-dependence of activation were not changed by LSA-A. The effect of LSA-A on Na current was abolished when high concentration of $Ca^{2+}$ buffer (10 mM BAPTA) was included in the pipette solution or when Ca2+ current was blocked by nicardipine (1 $\mu$M) in the bath solution.n.

• 한방안이비인후피부과학회지
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• 제21권3호
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• pp.52-68
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• 2008

Objectives and Methods : Salviae miltiorrhizae Radix (SMR) promotes blood circulation to remove blood stasis, cools the blood to relieve carbuncle, clears away heat from the heart and tranquilizes the mind. This study was designed to investigate the effects of SMR on immuno-potentiative action in terms of changes in the genetic profile of dendritic cells (DC) using by microarray analysis. Results and Conclusion: In this experiment, treatments with more than 250 ${\mu}g/ml$ upto 1000 ${\mu}g/ml$ of SMR elevated the proliferation rates of DC. Microscopic observations confirmed the tendency on proliferation rates. Expression levels of genes related with cellular methabolic process, cell communication, and macromolecule metabolic process were elevated by treatment with SMR in comparison of functional distribution in a Biological Process. In molecular functions, expression levels of genes related with receptor activation, nucleotide binding and nucleic acid binding were elevated. In cellular components, expression levels of genes related to cellular membrane-bound organelles were elevated. In addition, expression levels of genes related to Wnt signalling pathways and the glycerophospholipid metabolism were elevated through analysis using pathway analysis between up-and down-regulated genes in cells treated with SMR. Finally, genes related to JAK2, GRB2, CDC42, SMAD4, B2M, FOS and ESRI located the center of Protein interaction network of genes through treatment with SMR.