• Title/Summary/Keyword: Salmonella typhimurium Sal-13

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Antibiotic Susceptibility of Salmonella spp. Isolated From Diarrhoea Patients in Seoul From 1996 to 2001 (서울 시내 설사환자에서 분리한 살모넬라의 항생제 감수성의 년도별 변화 추이)

  • 박석기;박성규;정지헌;진영희
    • Journal of Food Hygiene and Safety
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    • v.17 no.2
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    • pp.61-70
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    • 2002
  • In order to investigate the classification and antibiotic resistance of Salmonella species,718 isolates were isolated from patient in Seoul from 1996 to 2001. The two hundred and ninety eight isolates (41.5%) were identified as Sal. Enteritidis, followed by Sal. Typhi 218 isolates (30.4%), and Sal. Typhimurium 87 isolates (12.1%). The identified Salmonella species were most resistant to tetracycline (32.7%), followed by streptomycin (28.0%), ticarcillin (18.1%) and ampicillin (12.4%). Among isolates,34.7% of Sal. Enteritidis were resistant to tetracycline, 32.3% to streptomycin,23.2% to ticarcillin,13.5% to ampicillin, respectively. 13.8% of Sal. Typhi were resistant to streptomycin,10.6% to tetracycline, respectively.66.7% of Sal. Typhimurium were resistant to tetracycline, 42.5% to streptomycin, 28.7% to ticarcillin, 26.4% to ampicillin and 17.2% to chloramphenicol, respectively. Of 718 isolates, 324 isolates (45.1%) were resistant to 1 or more drugs and 64 isolates (19.8%) were resistant to 1 drug, 132 isolates (40.7%) were resistant to 2 drugs,50 isolates (15.4%) were resistant to 3 drugs, 27 isolates (8.3%) to 4 drugs,27 isolates (8.3%) to 5 drugs,22 Isolates (6.8%) to 6 drugs. The most prevalent multiple resistant pattern was tetracycline-kanamycin (35.5%), followed by tetracycline-kanamycin-ticarcillin (8.3%), and tetracycline-kanamycin-ticarcillin-ampicillin (7.4%) . Antibiotic resistant rate of Sal. Typhimurium was 73.6%,1311owe4 by Sal. Enteritidis 53.7% and Sal. Typhi 19.3%. Most Sal. Enteritidis was resistant to 1 drug o.2 drugs, whereas Sal. Typhi. and Sal.. Typhunurium were more .resistant to 5 (16.7%) or 6 drugs (26.6%). The old generation antibiotics such as ampicillin, tetracycline, and streptomycin were annually more resistant than the new generation antibiotics such as ceftriaxone, ciprofloxacin or cefoxitin.

Effect of Clove (Eugenia caryophyllata Thumb) on the Survival of Listeria monocytogenes and Salmonella typhimurium during Cold Storage (저온저장중 Clove(Eugenia caryophyllata Thumb)가 Listeria monocytogenes와 Salmonella typhimurium의 생존에 미치는 영향)

  • 박찬성;최미애
    • Korean journal of food and cookery science
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    • v.13 no.5
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    • pp.602-608
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    • 1997
  • The antibacterial activity of low concentrations of clove (Eugenia caryophyllata Thumb) in culture broth against Listeria monocytogenes and Salmonella typhimurium was tested at 35, 5, and -20$^{\circ}C$. Tryptic soy broth (TSB) containing 0∼0.5% (w/v) of clove was inoculated with 10$\^$5/∼10$\^$7/ cell/ml of L. monocytogenes and S. typhimurium and incubated at each temperature. The growth of L. monocytogenes occured only after a prolonged lag period at 0.1% clove at 35$^{\circ}C$, while viabilities of the cells decreased by 1.4 and 3.3 log cycles at 0.3 and 0.5% clove, respectively. Growth of S. typhimurium occured at the presence of 0∼0.5% clove after a longer lag period with increasing concentration of clove at 35$^{\circ}C$. During refrigerated storage at 5$^{\circ}C$, the growth of L. monocytogenes occured after 6 days of lag period at 0.1% clove while viability of the cells were decreased during 24 days of storage. During frozen storage at -20$^{\circ}C$, the viability of L. monocytogenes and S. typhimurium decreased about 4 log cycles during 3 days of early period of storage at 0.1% clove. There were no major changes in the population of L. monocytogenes and S. typhimurium in TSB with different concentrations of clove during frozen storage.

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Bioserotype and drug resistance of Salmonella spp isolated from feces in zoo animals (동물원(動物園)의 야생동물(野生動物) 분변(糞便)에서 분리(分離)한 살모넬라균의 생물형(生物型), 혈청형(血淸型) 및 약제내성(藥劑耐性))

  • Youn, En-sun;Park, Seog-gee;Oh, Young-hee;Kim, Tae-jong
    • Korean Journal of Veterinary Research
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    • v.34 no.2
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    • pp.267-273
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    • 1994
  • Feces samples, obtained from zoo animals around Seoul, were examined for the isolation of Salmonella species, bioserotype and drug resistance for the prevention and treatment of salmonellosis, Salmonella spp were isolated 19(4.7%) from 408 samples of zoo animals. The subspecies in 19 Salmonella were all subspecies 1. The serological identification of Salmonella isolated were 31.6% in Sal typhimurium, 26.3% in Sal hadar, 21.1% in Sal muenchen, 15.8% in Sal enteritidis and 5.3% in Sal ayinde. The antibiotic resistance of Salmonella isolated were 13(68.4%) strains. The multiple resistant patterns of antibiotics in Salmonella were 2 drugs- and 3 drugs-resistance 30.8% respectively. The transferred rate of resistance to recipients(E coli ML 1410 $NA^r$) in Salmonella was 38.5%.

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Studies on the Comparison of Antibacterial Activity by Catechin Concentration in Green Tea Extract (녹차 추출물 중 카테킨 성분 농도에 따른 항균활성 비교 연구)

  • Choi Kyung-Min;Yun Young-Gab;Jiang Jing-Hua;Oh Sung-Su;Yang Hwan-Deok;Kim Hyoung-Jun;Jeon Byung-Hun;Park Hyun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.5
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    • pp.1233-1237
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    • 2005
  • Catechin products in green tea extract was prepared to investigate antibacterial activity on the pathogenic bacteria. Survival of pathogenic bacteria (MASA - methicillin resistant Staphylocouus aureus, E.coli O157 and S. typhimurium Sal-13) in tryptic soy agar(TSA) containing Catechin products powder incubated at various concentration was evaluated. TSA containing $0{\sim}2%(w/v)$ of Catechin products was inoculated approximately $10^4\;CFU/ml$ of pathogenic bacteria and incubated at $37^{\circ}C$ for 24 hours. The plate counting technique and clear zoon test were used to test survival effect of the Catechin products. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was derived from the survival curves of pathogenic bacteria. S. typhimurium Sal-13 was the most sensitive strain to Catechin products. This result suggested that Catechin products can be used as an effective natural antibacterial agent.

Antibacterial Activities of Medicinal Herbs on Salmonella and E. Coli (생약재가 살모넬라균 및 대장균의 항균에 미치는 효과)

  • Kim, Gye-Yeop;Nam, Ki-Won;Jang, Seung-Joo;Kim, Jung-Eun;Iim, Joung-Soo;Ahn, Byung-Sun;Kwun, Du-Seog;Jung, Hun-Woo;Cho, Kyoung-Oh
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.3
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    • pp.619-623
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    • 2008
  • The purpose of this study was to observe the effects of antibacterial activities on various medicinal herbs. Medicinal herbs used this study was Curcuma aromatica, Scutellaria baicalensis, Angelica dahurica, Platycodon grandiflorum, Houttuynia corolata, and Forsythia saxatilis. To evaluation of the antibacterial effect, we used S. gallinarum(ATCC 9184), S. typhimurium(SAL-13, France), S. enteritidis (ATCC 10376), Escherichia coli(E-62, O78), and S. gallinarum(E. coli). Extracts from each medicinal herbs divided into 100mg/ml, 50 mg/ml, 25 mg/ml, and 12.5 mg/ml. Antibacterial activities achieved by the paper disc test. Antibacterial activities was showed at extracts from Scutellaria baicalensis, Houttuynia corolata, Forsythia saxatilis, but little found at Curcuma aromatica, Angelica dahurica, Platycodon grandiflorum.