• The Korean Journal of Physiology
• /
• 제21권2호
• /
• pp.297-304
• /
• 1987

A study was carried out to find out the relationship between arginine vasopressin (AVP) release and plasma osmolality in 15 young men (age: 21.4 yr). After an overnight fasting, wale. (20 ml/kg) was imbibed, and venous blood and urine samples were collected every 30 min for 90 min. then 5% saline was infused (0.06 ml/min/kg) for 120 min. AVP was extracted on Sep-Pak column and measured by radioimmunoassay. Under basal condition, plasma osmolality (pOsm), AVP (pAVP) and aldosterone (pAldo) levels were 286.5 mOsm/kg, 1.1 pg/ml, and 140 pg/ml, respectively. pAVP became undetectable during maximum water diuresis, and increased in response to hypertonic saline infusion. pAVP level began to increase when pOsm was above 280 mOsm/kg. Changes in urinary AVP excretion (uAVP) was parallel to pAVP levels. The fall in pAVP was followed by a decrease in uAVP, uOsm and an increase in free water clearance, while the later rise in pAVP was followed by an increase in uAVP, uOsm and a decrease in free water clearance. When pooling all data together, relationships between pAVP and pOsm, and uAVP and uOsm were best expressed by an exponential relationship (r=0.78, 0.86, respectively). pAldo level decreased to 71 pg/ml after water ingestion, and decreased further to 30 pg/ml 2 hr after 5% saline infusion. Even at the same pNa, pAldo level during dehydration state was significantly higher than during hydration state. Negative exponential relation (r=-0.59) was observed between pAldo and pNa. Response to change in body fluid volume was greater in aldosterone than in AVP release.

• 터널과지하공간
• /
• 제26권3호
• /
• pp.166-180
• /
• 2016

본 연구에서는 TOUGH-FLAC 연동 해석기법을 이용하여 호주 오트웨이 실증프로젝트 염수층 내 이산화탄소 주입을 수치적으로 모델링하고 주변 지층에 야기되는 수리-역학적 거동 특성을 살펴보았다. 대상 부지의 물리검층, 시추코어 분석 및 지구통계기법을 통해 구현된 3차원 추계학적 지질모델을 바탕으로 해석모델을 작성하였으며, 이산화탄소의 주입과 이에 따른 수리-역학적 연계거동을 분석하였다. 총 30,000톤의 이산화탄소를 200일 동안 주입하는 경우, 주입공 주변의 압력 변화는 0.5 MPa 이하로 나타났으며, 주입 5일 경과 시 압력이 수렴하는 경향을 보였다. 지층 내 역학적 거동에는 큰 영향을 미치지 않을 것으로 판단되며, 주변 단층대로의 유동도 미미한 수준으로 나타났다.

• 한국토양비료학회지
• /
• 제44권1호
• /
• pp.84-90
• /
• 2011

It is imperative to study the hydrolysis of urea in high saline-sodic condition of a newly reclaimed tidal land in order to overcome the problems associated with use of urea fertilizer. The methodology adopted in this study tried to get a convenient way of estimating rate for N transformation needed in N fate and transport studies by reviewing pH and salt contents which can affect the microbial activity which is closely related to the rate of urea hydrolysis. The hydrolysis of urea over time follows first-order kinetics and soil urease activity in reclaimed soils will be represented by Michaelis-Menten-type kinetics. However, high pH and less microorganisms may delay the hydrolysis of urea due to decrease in urease activity with increasing pH. Therefore, the rate of urea hydrolysis should adopt $V_{max}$ referring enzyme activity ($E_0$) accounting for urease concentration which is indicative for urea hydrolysis, especially in a high saline and sodic soils.

• Restorative Dentistry and Endodontics
• /
• 제29권4호
• /
• pp.339-345
• /
• 2004

The aim of this study was to measure and compare the micro shear bond strengths of the following dentin bonding systems to the dentin surfaces under simulated pulpal pressure: All Bond $2^{\circledR},{\;}Second^{\circledR},{\;}AdheSE^{\circledR}$, Adper Prompt $L-Pop^{\circledR}$. The occlusal surfaces of 180 extracted human molars were prepared so the dentin bonding surfaces could be exposed. The teeth were randomly assigned to 3 equal groups of 60 each and subdivided. The dentin surfaces were treated with the above mentioned bonding system and resin composite cylinders were built up under a simulated pulpal pressure when saline (Group II) or diluted bovine serum (Group III) was used as the pulpal fluid. As a control. the same procedures were performed in the dried dentin surfaces (Group I). After one day of storage in water. the micro shear bond strengths were measured using an EZ tester. Group II and III showed significantly lower shear bond strength than Group I statistically (p < 0.05). $SEbond^{\circledR}{\;}and{\;}AdheSE^{\circledR}$ showed no difference among the different dentin condition. In the Adper Prompt $L-Pop^{\circledR}$. a simulated pulpal pressure were applied to the specimens using diluted bovine serum. which showed a higher strength than the specimens in which saline was used (p < 0.05).

• Journal of Plant Biotechnology
• /
• 제5권3호
• /
• pp.157-161
• /
• 2003

Proline is known as an osmoprotectant accumulating in response to salt and dehydration stresses. An increased level of proline is achieved by either an induced synthesis or a reduced degradation of proline. In an attempt to increase salt tolerance in carrot, a P5CS gene from mothbean was introduced via an Agrobacterium-mediated transformation. The resulting carrot cells and the regenerated plants containing the transgene showed increased levels of proline compared to nontransgenics. The transgenic cell line, Pj2 showed about 6 times increased degree of tolerance determined by relative growth after a treatment in 250 mM NaCl. In facts, due to the retarded growth shown in non-saline condition, Pj2 cells grow only about 1.2 times better than nontransgenic control under salt stress condition. Taken together, it appears that a P5CS is a key enzyme in proline biosynthesis and the increased accumulation of proline by overexpression of the enzyme is enough to enhance tolerance to salt stress in carrot.

• 대한치과교정학회지
• /
• 제32권3호
• /
• pp.227-234
• /
• 2002

본 연구는 조골세포의 특성을 가지고 있는 G292세포주를 이용하여 세포막 이온통로에 대한phorbol ester의 효과를 조사하여 protein kinase C (PCK)의 이온통로에 대한 작용기전을 밝히고자 하였다. Patch clamp 기법을 이용하여 G292 세포에서 cell-attached configuration으로 단일이온통로의 활동을 관찰하고 Phorbol 12, 13-dibutyrate (PDBu)의 효과를 관찰하였다. 안정상태 G292 세포에서 cell-attached 모드로 세포막의 단일이 온통로 활동을 관찰한 결과 45pS의 $K^+$통로가 특징 적으로 우세하였다. 유리 전극 내부에 세포내 액과 세포외 액을 사용하여 전류-전압의 관계를 조사한 결과, 세포내 액을 사용하는 경우에는 역전전압이 5.5mV이었으며 세포외액을 사용하는 경우에는 -27mV이었다. PDBu는 45pS의 이온통로를 10nM이상의 농도에서 이온통로의 열릴 확률을 증가시켰으며 PKC억제제인 staurosporine 10nM에 의하여 차단되는 특성을 보였다. PDBu는 45pS의 이온통로에 작용하여 전류-전압의 관계에서 역전전압을 음의 방향으로 이동시켰으며 동일한 막전압에서 단일이온통로의 전류 크기를 증가시켰다. G292세포에서 PDBu에 의하여 PKC가 활성화되는 것을 western blot으로 확인한 결과 PDBu 0.luM은 세포질에서 세포막으로 PKC translocation을 유의하게 증가시키는 것을 확인하였다. 이상의 결과는 G292세포에서 phorbol ester의 일종인 PDBu가 세포내 PKC를 활성화시켜 45pS의 이온통로를 활성화시키며 이러한 작용의 결과로 세포막전압의 변화가 세포의 기능을 조절할 것으로 사료된다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.316-316
• /
• 2017

The objective of this study is to estimate quantitatively soil salinity and soil loss at upland soils in agriculture land region in Saemangeum reclaimed land on the south Korea coasts. Soil loss and soil salinity are the most critical problem at reclaimed tidal saline soil in Korea. The several thematic maps of research area such as land cover map, topographic and soil maps, together with tabular precipitation data used for soil erosion and soil salinity calculation. Meteorological data were measured directly as air temperature, wind speed, solar radiation, and precipitation. The experiment was conducted 2% sloped lysimeter ($5.0m{\times}20.0m$) with 14 treatments and it were separated by low salinity division (LSD) and high salinity division (HSD) install. The cation content in ground water increased during time course, but in the case of land surface water the content was variable, and $K^+$ was lower than that of $Na^+$ and $Mg^{2+}$. At the LSD under rainproof condition, the salinity was directly proportional to soil water content, but at the HSD the tendency was no reversed. In condition of rainproof, the amount of soil salinity was higher at the HSD than at the LSD. Positive correlation was obtained between the soil water content and available phosphorous content at the rainfall division, but there was no significance at the surface soil of the rainproof division. Sodium adsorption ratio and anion contents in soil were repressed in the order of vinyl-mulching > non-mulching > bare field. According to the result of analyzing soil loss, soil loss occurred in a vinyl-mulching, a non-mulching and a bare field in size order, and also approximately 11.2 ton/ha soil loss happened on the reclaimed land area. The average soil loss amount by the unit area takes place in a non-mulching and bare field a lot. Our results indicate that soluble salt control and soil erosion are critical at reclaimed tidal saline soil and the results can provide some useful information for deciding management plans to reduce soil loss and salt damage for stable crop production and diverse utilization or cultivation could be one of the management options to alleviate salt damage at reclaimed tidal saline soil in Korea.

• 대한한방소아과학회지
• /
• 제24권3호
• /
• pp.106-120
• /
• 2010

Objectives: Sanjointang has been clinically used much for treating sleeplessness. However, the effects of Sanjointang in artificial sleep deprivation situations are not known. The purpose of this study is to evaluate the heart rate, left ventricular systolic pressure, left ventricular diastolic pressure, +dp/dt maximum, -dp/dt maximum, and -dp/dt / +dp/dt ratio which are related to the hemodynamic functions of the heart by using sleep-deprived Sparague-Dawley rats, in order to clarify the impact of Sanjointang on hemodynamic functions of the heart of sleep deprived rats. Methods: Eighteen hearts were removed from the male Sparague-Dawley rats weighting about 180g were perfused by the Langendorff technique with modified 37 Krebs-Henseleit's buffer solution at a constant perfusion pressure (60mmHg). They were randomly assigned to one of the following three groups, 1) Normal group (those which did not have sleep deprivation and received normal saline administration), 2) Control group (sleep deprived and normal saline administered), 3) Sample group (sleep deprived and Sanjointang was administered). Control and sample groups rats were deprived 96 hours of sleep by using the modified multiple platform technique. Heart rate, left ventricular systolic pressure, left ventricular diastolic pressure, +dp/dt maximum, -dp/dt maximum, -dp/dt / +dp/dt ratio were evaluated at baseline after the administration of either normal saline or Sanjointang. Results: The heart rate and -dp/dt / +dp/dt ratio was significantly decreased in rats with 96 hours of sleep deprived significantly decreased. The change in the heart rate after administering Sanjointang did not show any significant difference. The left ventricular systolic pressure of the removed heart significantly decreased due to Sanjointang administration, while the left ventricular diastolic pressure significantly increased (p<0.05). The +dp/dt maximum and -dp/dt maximum both significantly decreased in the removed heart after administering Sanjointang. (p<0.05). There was no significant difference observed in the -dp/dt / +dp/dt ratio after administering Sanjointang. Conclusions: According to the results above, sleep deprivation significantly decreases heart rate and -dp/dt / +dp/dt ratio. This is considered as a result of exhaustion due to accumulation of fatigue. Meanwhile, Sanjointang reduced left ventricular systolic pressure and raised left ventricular diastolic pressure, and relieved the contractility and relaxation of the myocardium. Consequently, this reduces the burden of the heart and creates a relatively stabilized heart condition similar to a sleeping condition.

• 한국해양학회지
• /
• 제30권4호
• /
• pp.237-249
• /
• 1995

대한 해협 횡단면 상의 수온, 염분 및 해류의 구조를 이해하기 위하여 1994년 5월 2일부터 20일 까지 현장 조사를 수행하였다. 연구선 탐양호를 이용하여 상세한 CTD 수 직 profile과 ADCP 측정 기록을 얻었으며 두 개의 시간차(15일과 25시간)에 대한 평균 장과 변동장을 분석하였다. 대한 해협 중앙부에는 서로 다른 두 개의 수기 즉 동측의 고온 고염수와 서측의 저온저염수가 서로 인접하면서 대단히 뚜렷한 연안 전선을 형성 하고 있다. 이 고운 고염 수괴가 약 보름에 걸쳐서 서진하면서 수온 염분장을 크게 변 화 시키고 있음을 관찰할 수 있었다. 또한 중층에서는 수시간대의 짧은 주기로서도 수 온과 염분이 변화하고 있었으며, 이 현상은 EOF분석에서 제 1고유 모드로 나타나며 그 것은 50%이상의 전체 분산을 점하고 있다. 대한 해협에서의 흐름은 조류에 의하면 심 하게 영향을 받고 있으며 해협 중앙부에서 사리와 조금 때 각각 최대 80-90 및 60-70 cm/s의 크기를 보여 준다. 강한 남향의 조류에 의해 때에 따라서는 북향하는 대마 난 류의 존재가 완전히 가려 보이지 않기도 하였다. 25시간 연속 해류 관측 결과의 조화 분석 결과사리와 조금 때 평균, 단일주조류 및 일일주조류의 크기가 서로 비슷함을 알 수 있었다. 대한 해협 서수도를 통과하는 용접수송량은 조금 때 2.1 사리 때에 3.4 Sv 으로 추정되었다.

• 한국토양비료학회지
• /
• 제20권4호
• /
• pp.359-367
• /
• 1987

수종(數種)의 유기산(有機酸), 당류(糖類)(단당(單糖), 복합당(複合糖)) 및 수도근계분비물질(水稻根系分泌物質)에 대(對)한 협생질소고정균(協生窒素固定菌)의 화학주화성(化學走化性)을 검토(檢討)하기 위하여 우리나라 서해간척지(西海干拓地)의 잡초(雜草) 및 수도(水稻) 근조직(根組織)에서 분리동정(分離同定)한 균(菌)을 공시(供試)하여 시험(試驗)하였다. 공시(供試)한 협생질소고정균중(協生窒素固定菌中) Azospirillum lipoferum Ecc 3-1은 유인물질(誘引物質)이 있는 방향(方向)으로 이동(移動)하기 위한 천이환(遷移環)을 만들었으나 Agrobacterium radiobacter Ecc 1-1과 Pseudomonas sp Ecc 4-1은 천이환(遷移環)을 만들지 않했다. 당류(糖類) 및 유기산(有機酸)에 대(對)한 공시세균(供試細菌)의 화학주화성(化學走化性)은 D(+)-glucose나 D(+)-fructose 보다 malic acid나 citric acid에 대(對)한 주화성(走化性)이 높았다. 특(特)히 복합당(複合糖)인 D-galactouronic acid와 L-aspartate는 공시(供試)한 타종(他種)의 당류(糖類)나 유기산(有機酸)보다 가장 높은 주화력(走化力)을 보였다. 수도근분비물질(水稻根分泌物質)에 대(對)한 공시협생질소고정균(供試協生窒素固定菌)의 주화성(走化性)을 수도품종(水稻品種)과 세균간(細菌間)에 서로 상이(相異)한 교호관계(交互關係)를 보였다.