• Title/Summary/Keyword: Safety-2

Search Result 21,454, Processing Time 0.055 seconds

Application for Identification of Food Raw Materials by PCR using Universal Primer (일반 프라이머를 이용한 PCR의 식품원료 진위 판별에 적용)

  • Park, Yong-Chjun;Jin, Sang-Ook;Lim, Ji-Young;Kim, Kyu-Heon;Lee, Jae-Hwang;Cho, Tae-Yong;Lee, Hwa-Jung;Han, Sang-Bae;Lee, Sang-Jae;Lee, Kwang-Ho;Yoon, Hae-Seong
    • Journal of Food Hygiene and Safety
    • /
    • v.27 no.3
    • /
    • pp.317-324
    • /
    • 2012
  • In order to determine an authenticity of food ingredient, we used DNA barcode method by universal primers. For identification of animal food ingredients, LCO1490/HCO2198 and VF2/FISH R2 designed for amplifying cytochrome c oxidase subunit1 (CO1) region and L14724/H15915 for cytochrome b (cyt b) region on mitochondrial DNA were used. Livestock (cow, pig, goat, sheep, a horse and deer) was amplified by LCO1490/HCO 2198, VF2/FISH R2 and L14724/H15915 primers. Poultry (chicken, duck, turkey and ostrich) was amplified by LCO1490/HCO 2198 and VF2/FISH R2 primers. But, Fishes (walleye pollack, herring, codfish, blue codfish, trout, tuna and rockfish) were only amplified by VF2/FISH R2 primers. For plant food ingredients, 3 types of primers (trnH/psbA, rpoB 1F/4R and rbcL 1F/724R) have been used an intergenic spacer, a RNA polymerase beta subunit and a ribulose bisphosphate carboxylase region on plastid, respectively. Garlic, onion, radish, green tea and spinach were amplified by trnH/psbA, rpoB 1F/4R and rbcL 1F/724R. The PCR product sizes were same by rpoB 1F/4R and rbcL 1F/724R but, the PCR product size using trnH/psbA primer was different with others for plants each. We established PCR condition and universal primer selection for 17 item's raw materials for foods and determine base sequences aim to PCR products in this study. This study can apply to determine an authenticity of foods through making an comparison between databases and base sequences in gene bank. Therefore, DNA barcode method using universal primers can be a useful for species identification techniques not only raw materials but also processed foods that are difficult to analyze by chemical analysis.

The Development of the Safety Valve for LP Gas Cylinder Using the Sintered Metal (소결금속을 이용한 LPG 용기용 안전 밸브의 개발)

  • Rhim, Jong-Kuk
    • Journal of the Korean Society of Safety
    • /
    • v.21 no.1 s.73
    • /
    • pp.48-52
    • /
    • 2006
  • This study is a research of safety valve development for LP gas cylinder which use sintering metal. Re-searcher wishes to apply technology of sintering metal for safety valve development and do gas flow control. The basis of this study is most suitable fluid examination that to reduce gas accident. This research concluded following results. 1. When press pin length is 42mm to 45mm powder quantity is 0.25g, in case of press pin length 36mm to 42mm powder quantity is 0.2g, displayed fluid optimization. 2. When press pin length is 39mm and powder quantity is 0.25g, press pressure displayed fluid optimization at all interests from $1.2\;tons/cm^{2}\;to\;2\;tons/cm^{2}$. 3. When apparent density is about $5.0g/cm^{3}\;to\;4.5g/cm^{3}$, fluid optimization becomes.

Management Factors Associated with Health and Safety Education in Korean Manufacturing Companies (산업장 안전보건교육 관리요인)

  • Lee Myung-Sun;Lee Gwan-Hyung;Park Kyoung-Ok
    • Korean Journal of Health Education and Promotion
    • /
    • v.23 no.2
    • /
    • pp.121-140
    • /
    • 2006
  • Objectives: Safety is a primary health promotion issue in worksite because injury induces multi-fold loss of the human and economic resources to profit organization. The purposes of this study were to describe worksite health and safety education and management status in Korean manufacturing companies. Methods: The original population size of Korean manufacturing industry in 2004 was 74,398 and 2,960 factories were selected by the multiple stratified sampling method for this study. The health and safety manager or representatives of the selected 2,960 companies successfully finished in the face-to-face interview survey about company's general characteristics, health and safety management style, health and safety education hours conducted by the Korean Occupational Safety and Health Agency. Results: The manufacturing companies in Seoul and Kyunggi areas, small size, and clothes and press industries were related to low health and safety management and education status. The companies which assigned at least one safety manager were 70.5% and which had a health and safety room within the company were only 9.3%. The companies which took the health and safety education for their regular blue-collar employees more than the legal education hours were under 56.1% and the percentage of the companies which took their health and safety education for newcomers less than the legal limits was lower than any other types of health and safety education in workplace. The significant strong workplace health and safety management variables in predicting employee health and safety education were psycho-social variables such as the company own health and safety regulation and the workplace health and safety management committee organization. rather than physical variables such as health manager employment, safety manager employment. Conclusions: Systematic and legal approaches are effective to encourage workplace health and safety education, specifically, through sustaining health and safety managers and building the company-wide health and safety management system. Furthermore, theses approaches should primarily focus on the small companies of which sizes were under 50.

A Study on the Safety Requirements Establishment through System Safety Processes (시스템 안전성평가를 통한 효율적 요건 도출방안 연구)

  • Yoo, Seung-woo;Jung, Jinpyong;Yi, Baeck-Jun
    • Journal of Aerospace System Engineering
    • /
    • v.7 no.2
    • /
    • pp.29-34
    • /
    • 2013
  • Safety requirements for aircraft and system functions include minimum performance constraints for both availability and integrity of the function. These safety requirements should be determined by conducting a safety assessment. The depths and contents of aircraft system safety assessment vary depending on factors such as the complexity of the system, how critical the system is to flight safety, what volume of experience is available on the type of system and the novelty and complexity of the technologies being used. Requirements that are defined to prevent failure conditions or to provide safety related functions should be uniquely identified and traceable through the levels of development. This will ensure visibility of the safety requirements at the software and electronic hardware design level. This paper has prepared to study on promoting the efficiency of establishing hierarchical safety requirements from aircraft level function to item level through system safety processes.

Relationship between Risk-causing Factors Perceived by Telecommunications Workers and Safety Motivation and Behavior

  • Kwang-Seup Byun;Jin-woo Jung
    • Journal of information and communication convergence engineering
    • /
    • v.21 no.2
    • /
    • pp.174-184
    • /
    • 2023
  • In this study, the structural relationship among risk factors, safety motivation, and safety behavior perceived by telecommunications workers was empirically verified. A survey was conducted on field workers at S telecommunications companies in Korea, and the following major results were obtained. First, among the risk factors perceived by telecommunications workers, personal, cultural, and institutional factors were found to have significant positive effects on workers' safety motivation. Second, workers' safety motivation had a significant positive effect on safety behavior. Third, among the risk factors of telecommunications companies, personal, cultural, and institutional factors did not significantly affect workers' safety behavior. Fourth, all risk-causing factors perceived by workers, such as personal, cultural, and institutional factors, indirectly affected safety behavior through safety motivation. This study presents the importance of perceiving risk factors in the workplace to prevent and alleviate industrial accidents by examining the effects of riskcausing factors perceived by carrier workers on safety motivation and behavior.

The Status of Safety Accident and Safety Health Education and Risk Factors of Safety Accident among the selected High School Students (일부 고등학생들의 안전보건교육 및 안전사고 실태와 관련요인)

  • 한성현
    • Korean Journal of Health Education and Promotion
    • /
    • v.19 no.1
    • /
    • pp.1-15
    • /
    • 2002
  • In this study, it is aimed to clarify the status of safety accident and safety health education and risk factors on safety accidents among the selected high school students. The study is accomplished by the survey which includes a total of 558 students sampled from the six high school. The survey took for 10 days from the date of 3th July 2000 to 13th July 2000 and the results were as follows; 1. The rate of experience of safety health education during last one year was 26.4% and the rate of accident during last one month was 20.6%. 2. The risk factors of safety accident during last one year were understanding degree of safety health education(R.R.=2.81), experience of smoking(R.R.=2.55), educational level of mother (R.R=l.63) From the results of this study, we are to propose as follow; Because the purpose of safety health education is the practice, it is important to induce changing the recognition and behavior. Therefore the government have to build up experience place, to bring up professional teacher of safety health education. It need to develope a reference of each subject and to utilize various media. Especially it need to take of education comprehensive health and environmental inducing safety health subject.

A Study on the Subchronic Inhalation Toxicity of 2-Butanethiol Using SD Rats (2-부탄티올의 SD Rats를 이용한 아만성 흡입독성연구)

  • Kim, Hyeon-Yeong;Lee, Sung-Bae;Lim, Cheol-Hong;Cho, Hae-Won;Kang, Min-Gu;Lee, Jun-Yeon
    • Journal of Korean Society of Occupational and Environmental Hygiene
    • /
    • v.17 no.3
    • /
    • pp.181-191
    • /
    • 2007
  • With the 2-Butanethiol, which is an unidentified inhalation toxic material, acute inhalation toxicity was tested with SD rats. The $LC_{50}$ was evaluated to be 2,500 ppm (9.22 mg/L) or higher which falls under the criteria of acute toxicity Category 3 (500<$LC_{50}$<2,500 ppm) in the Industrial Safety and Health Act. In the subchronical inhalation toxicity test by 0, 25, 100, and 400 ppm, 6 hours a day, 5 days a week, for 13 weeks repeated exposure, though no death or particular clinical presentation was observed, in the female 25 and 400 ppm group, including weight change, and in each concentration group including 400 ppm, change of feed rate, eye stimulation, motility change in male group, and lesions in blood and blood biochemical were observed. In the internal organs weight, 25, 100, and 400 ppm groups in male and 400 ppm group in female showed significant (p<0.05) changes in kidney, liver, thymus, and lung. In the pathological tissue test, severe cortical tubular hyaline droplets were observed in the male 400 ppm group, and all male rats of 400 ppm group and 2 female individuals showed tubular degeneration/regeneration accompanied with pigmentation, showing that the target organs of inhalation exposure of 2-Butanethiol are spleen, kidney, nasal cavity, and adrenal. Through the tests, the NOEL of 2-Butanethiol was evaluated to be 25 ppm (0.092 mg/L) or less for both male and female.

Oxidative stress impairs the meat quality of broiler by damaging mitochondrial function, affecting calcium metabolism and leading to ferroptosis

  • Chen, Zuodong;Xing, Tong;Li, Jiaolong;Zhang, Lin;Jiang, Yun;Gao, Feng
    • Animal Bioscience
    • /
    • v.35 no.10
    • /
    • pp.1616-1627
    • /
    • 2022
  • Objective: This work was conducted to investigate the effects of oxidative stress on meat quality, mitochondrial function, calcium metabolism and ferroptosis of broilers. Methods: In this study, a total of 144 one-day-old male Ross 308 chicks were divided into 3 groups (control group, saline group, and hydrogen peroxide [H2O2] group) with 6 replicates of 8 broilers each. The study lasted for 42 d. The broilers in the saline and H2O2 groups were intraperitoneally injected with 0.75% saline and 10.0% H2O2 on the 16th and 37th day of the experimental period respectively, the injection volumes were 1.0 mL/kg of broiler body weight. On the 42nd day of the experimental period, two chicks were randomly selected from each cage, a total of thirty-six chicks were stunned by electric shock and slaughtered to collect breast muscle samples. Results: The H2O2 exposure reduced pH value, increased drip loss and shear force of breast meat (p<0.05), impaired the ultrastructure and function of mitochondria. The H2O2 exposure damaged the antioxidant system in mitochondria, excessive reactive oxygen species carbonylation modified calcium channels on mitochondria, which impaired the activities of key enzymes on calcium channel, resulted in the increased calcium concentration in cytoplasm and mitochondria (p<0.05). In addition, the H2O2 exposure increased the iron content and lipid peroxidation (p<0.05), which induced ferroptosis. Conclusion: Oxidative stress could impair meat quality by causing mitochondrial dysfunction, resulting in calcium metabolism disorder and ferroptosis.

A Study on the Safety Consciousness of Fire-fighting Sector Workers - Based on Fire-fighting Sector Workers in Chung-nam - (소방분야 종사자들의 안전의식에 관한 연구 - 충남지역 소방분야 종사자를 중심으로 -)

  • Hong, Young-Ho
    • Fire Science and Engineering
    • /
    • v.29 no.2
    • /
    • pp.64-72
    • /
    • 2015
  • This study was carried out for the purpose of awareness survey for safety and fire fighting safety of lives and general safety for fire-fighting sector workers of Chung-nam region. The study was carried out in the questionnaire survey. The questionnaire respondents were 534 people such as fire-fighting public officials, fire-fighting company worker and safety-related public officials. The results of the questionnaire were subjected to statistical processing, such as frequency analysis using SPSS win 19.0. The reliability of the questionnaire in this study is a Cronbach' ${\alpha}=0.710{\sim}0.807$. According to the survey results, Only 15% of the surveyed are think that peoples safety consciousness was high, the remaining respondents think the low level of safety consciousness of peoples. According to the findings of the fire-fighting related safety consciousness, fire-fighting public officials safety consciousness level was 4.2 at standard 5 and safety-related public officials was 3.59. Safety consciousness level order was fire-fighting public officials > Fire-fighting company workers > safety-related public officials. According to the survey, the efforts to decrease the difference in recognition for safety is required.

The Relationship between Empowering Leadership, Directive Leadership, Safety Communication, and Safety Behavior for Air Force Combat Pilots: The Moderated Mediating Effect of Safety Motivation (공군 전투조종사의 임파워링 리더십, 지시적 리더십, 안전의사소통 및 안전행동과의 관계: 안전동기의 조절된 매개효과)

  • Kong, Manseok;Park, Jiyoung;Shin, Yonghwan;Sohn, Young Woo
    • Journal of the Korean Society for Aviation and Aeronautics
    • /
    • v.26 no.2
    • /
    • pp.8-30
    • /
    • 2018
  • The purpose of this study was to investigate the relationship between empowering leadership, directive leadership, safety communication, and safety behavior. Also, we examined whether safety motivation had a moderated mediating effect on this relationship. We collected the surveys from 343 combat pilots of the Republic of Korea Air Force for the analysis. Our structural equation modeling analysis revealed that there was a positive relationship between empowering leadership and safety behavior, and safety communication fully mediated the relationship. However, there is no relationship between directive leadership and safety behavior. Also, our results showed that safety motivation moderated the relationship between empowering leadership and safety communication, but not the relationship between directive leadership and safety communication. Furthermore, the relationship between empowering leadership and safety behavior through safety communication varied significantly with the degree of safety motivation, such that the higher the safety motivation, the higher the impact of empowering leadership on safety behavior through safety communication. Finally, we discussed the implications of these results, study limitations, and practical suggestions for future research.