• 한국식품위생안전성학회지
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• 제27권3호
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• pp.317-324
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• 2012

본 연구는 식품원료의 진위여부를 판별하기 위한 시험법으로 일반 프라이머를 이용한 DNA barcode 기법을 도입하였다. 동물성식품원료의 경우 미토콘드리아 DNA 중 cytochrome oxidase subunit I(COI) 부위 검출을 위하여 디자인된 프라이머(LCO1490/HCO2198 및 VF2/FISH R2)와 cytochrome b(cyt b) 부위 검출을 위하여 디자인된 프라이머(L14724/H15915)를 사용하였다. 상기 3 종류의 프라이머를 사용하여 가축류 6종(소, 돼지, 염소, 양, 말 및 사슴), 가금류 4종(닭, 오리, 칠면조 및 타조), 어류 7종(명태, 대구, 청대구, 청어, 송어, 다랑어 및 우럭)을 대상으로 PCR 후 전기영동하여 예상되는 PCR 산물의 생성 유무를 확인하였다. 가축류 6종에 대하여는 LCO1490/HCO2198, VF2/FISH R2 및 L14724/H15915 프라이머를 사용한 경우 COI 및 cyt b가 모두 검출되었으며, 가금류 4종은 LCO1490/HCO2198 및 VF2/FISH R2 프라이머를 사용한 경우만 COI이 검출되었다. 또한 어류 7종은 VF2/FISH R2 프라이머를 사용한 경우에만 COI 부위가 검출됨을 확인하였다. 식물의 경우 엽록체 DNA 부위를 이용하여 디자인된 3 종류의 프라이머(trnH/psbA, rpoB 1F/4R 및 rbcL 1F/724R)를 사용하였다. 각각의 프라이머를 이용하여 식물 5종(마늘, 양파, 무, 녹차 및 시금치)에 대하여 실험한 결과 3종류의 프라이머에서 PCR의 산물을 모두 확인하였으며 trnH/psbA 프라이머의 경우 식물 종마다 PCR 산물의 크기는 다르게 검출되었다. 본 연구에서는 17종의 식품원료별 일반 프라이머 및 PCR 조건을 확립하였으며, 생산된 PCR 산물을 대상으로 염기서열을 결정하고 유전자은행에 있는 염기서열과 DB 비교 분석을 통하여 식품에 사용된 원료의 진위여부 판별에 적용이 가능할 것으로 기대된다.

• 한국안전학회지
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• 제21권1호
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• pp.48-52
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• 2006

This study is a research of safety valve development for LP gas cylinder which use sintering metal. Re-searcher wishes to apply technology of sintering metal for safety valve development and do gas flow control. The basis of this study is most suitable fluid examination that to reduce gas accident. This research concluded following results. 1. When press pin length is 42mm to 45mm powder quantity is 0.25g, in case of press pin length 36mm to 42mm powder quantity is 0.2g, displayed fluid optimization. 2. When press pin length is 39mm and powder quantity is 0.25g, press pressure displayed fluid optimization at all interests from $1.2\;tons/cm^{2}\;to\;2\;tons/cm^{2}$. 3. When apparent density is about $5.0g/cm^{3}\;to\;4.5g/cm^{3}$, fluid optimization becomes.

• 보건교육건강증진학회지
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• 제23권2호
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• pp.121-140
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• 2006

Objectives: Safety is a primary health promotion issue in worksite because injury induces multi-fold loss of the human and economic resources to profit organization. The purposes of this study were to describe worksite health and safety education and management status in Korean manufacturing companies. Methods: The original population size of Korean manufacturing industry in 2004 was 74,398 and 2,960 factories were selected by the multiple stratified sampling method for this study. The health and safety manager or representatives of the selected 2,960 companies successfully finished in the face-to-face interview survey about company's general characteristics, health and safety management style, health and safety education hours conducted by the Korean Occupational Safety and Health Agency. Results: The manufacturing companies in Seoul and Kyunggi areas, small size, and clothes and press industries were related to low health and safety management and education status. The companies which assigned at least one safety manager were 70.5% and which had a health and safety room within the company were only 9.3%. The companies which took the health and safety education for their regular blue-collar employees more than the legal education hours were under 56.1% and the percentage of the companies which took their health and safety education for newcomers less than the legal limits was lower than any other types of health and safety education in workplace. The significant strong workplace health and safety management variables in predicting employee health and safety education were psycho-social variables such as the company own health and safety regulation and the workplace health and safety management committee organization. rather than physical variables such as health manager employment, safety manager employment. Conclusions: Systematic and legal approaches are effective to encourage workplace health and safety education, specifically, through sustaining health and safety managers and building the company-wide health and safety management system. Furthermore, theses approaches should primarily focus on the small companies of which sizes were under 50.

• 항공우주시스템공학회지
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• 제7권2호
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• pp.29-34
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• 2013

Safety requirements for aircraft and system functions include minimum performance constraints for both availability and integrity of the function. These safety requirements should be determined by conducting a safety assessment. The depths and contents of aircraft system safety assessment vary depending on factors such as the complexity of the system, how critical the system is to flight safety, what volume of experience is available on the type of system and the novelty and complexity of the technologies being used. Requirements that are defined to prevent failure conditions or to provide safety related functions should be uniquely identified and traceable through the levels of development. This will ensure visibility of the safety requirements at the software and electronic hardware design level. This paper has prepared to study on promoting the efficiency of establishing hierarchical safety requirements from aircraft level function to item level through system safety processes.

• Journal of information and communication convergence engineering
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• 제21권2호
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• pp.174-184
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• 2023

In this study, the structural relationship among risk factors, safety motivation, and safety behavior perceived by telecommunications workers was empirically verified. A survey was conducted on field workers at S telecommunications companies in Korea, and the following major results were obtained. First, among the risk factors perceived by telecommunications workers, personal, cultural, and institutional factors were found to have significant positive effects on workers' safety motivation. Second, workers' safety motivation had a significant positive effect on safety behavior. Third, among the risk factors of telecommunications companies, personal, cultural, and institutional factors did not significantly affect workers' safety behavior. Fourth, all risk-causing factors perceived by workers, such as personal, cultural, and institutional factors, indirectly affected safety behavior through safety motivation. This study presents the importance of perceiving risk factors in the workplace to prevent and alleviate industrial accidents by examining the effects of riskcausing factors perceived by carrier workers on safety motivation and behavior.

• 보건교육건강증진학회지
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• 제19권1호
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• pp.1-15
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• 2002

In this study, it is aimed to clarify the status of safety accident and safety health education and risk factors on safety accidents among the selected high school students. The study is accomplished by the survey which includes a total of 558 students sampled from the six high school. The survey took for 10 days from the date of 3th July 2000 to 13th July 2000 and the results were as follows; 1. The rate of experience of safety health education during last one year was 26.4% and the rate of accident during last one month was 20.6%. 2. The risk factors of safety accident during last one year were understanding degree of safety health education(R.R.=2.81), experience of smoking(R.R.=2.55), educational level of mother (R.R=l.63) From the results of this study, we are to propose as follow; Because the purpose of safety health education is the practice, it is important to induce changing the recognition and behavior. Therefore the government have to build up experience place, to bring up professional teacher of safety health education. It need to develope a reference of each subject and to utilize various media. Especially it need to take of education comprehensive health and environmental inducing safety health subject.

• 한국산업보건학회지
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• 제17권3호
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• pp.181-191
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• 2007

With the 2-Butanethiol, which is an unidentified inhalation toxic material, acute inhalation toxicity was tested with SD rats. The $LC_{50}$ was evaluated to be 2,500 ppm (9.22 mg/L) or higher which falls under the criteria of acute toxicity Category 3 (500<$LC_{50}$<2,500 ppm) in the Industrial Safety and Health Act. In the subchronical inhalation toxicity test by 0, 25, 100, and 400 ppm, 6 hours a day, 5 days a week, for 13 weeks repeated exposure, though no death or particular clinical presentation was observed, in the female 25 and 400 ppm group, including weight change, and in each concentration group including 400 ppm, change of feed rate, eye stimulation, motility change in male group, and lesions in blood and blood biochemical were observed. In the internal organs weight, 25, 100, and 400 ppm groups in male and 400 ppm group in female showed significant (p<0.05) changes in kidney, liver, thymus, and lung. In the pathological tissue test, severe cortical tubular hyaline droplets were observed in the male 400 ppm group, and all male rats of 400 ppm group and 2 female individuals showed tubular degeneration/regeneration accompanied with pigmentation, showing that the target organs of inhalation exposure of 2-Butanethiol are spleen, kidney, nasal cavity, and adrenal. Through the tests, the NOEL of 2-Butanethiol was evaluated to be 25 ppm (0.092 mg/L) or less for both male and female.

• Animal Bioscience
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• 제35권10호
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• pp.1616-1627
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• 2022

Objective: This work was conducted to investigate the effects of oxidative stress on meat quality, mitochondrial function, calcium metabolism and ferroptosis of broilers. Methods: In this study, a total of 144 one-day-old male Ross 308 chicks were divided into 3 groups (control group, saline group, and hydrogen peroxide [H₂O₂] group) with 6 replicates of 8 broilers each. The study lasted for 42 d. The broilers in the saline and H₂O₂ groups were intraperitoneally injected with 0.75% saline and 10.0% H₂O₂ on the 16th and 37th day of the experimental period respectively, the injection volumes were 1.0 mL/kg of broiler body weight. On the 42nd day of the experimental period, two chicks were randomly selected from each cage, a total of thirty-six chicks were stunned by electric shock and slaughtered to collect breast muscle samples. Results: The H₂O₂ exposure reduced pH value, increased drip loss and shear force of breast meat (p<0.05), impaired the ultrastructure and function of mitochondria. The H₂O₂ exposure damaged the antioxidant system in mitochondria, excessive reactive oxygen species carbonylation modified calcium channels on mitochondria, which impaired the activities of key enzymes on calcium channel, resulted in the increased calcium concentration in cytoplasm and mitochondria (p<0.05). In addition, the H₂O₂ exposure increased the iron content and lipid peroxidation (p<0.05), which induced ferroptosis. Conclusion: Oxidative stress could impair meat quality by causing mitochondrial dysfunction, resulting in calcium metabolism disorder and ferroptosis.

• 한국화재소방학회논문지
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• 제29권2호
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• pp.64-72
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• 2015

본 연구는 충남지역 소방분야종사자를 대상으로 일반안전, 생활안전 그리고 소방안전에 대한 의식조사를 목적으로 실시하였다. 연구는 설문조사로 진행되었으며 설문응답자는 소방공무원, 소방업체종사자, 안전관련 공무원 등 534명이다. 설문결과는 SPSS win 19.0을 사용하여 빈도분석 등의 통계처리를 하였다. 본 연구에서 설문의 신뢰도는 Cronbach' ${\alpha}=0.710{\sim}0.807$이다. 조사결과에 의하면 국민의 안전의식수준이 높다고 생각하는 응답자가 전체 응답자의 15%로 소방분야종사자들은 국민의 안전의식수준이 낮다고 인식하고 있었다. 소방안전의식에 대해 소방공무원은 5점 기준 4.2의 인식을 가지며, 안전관련 공무원은 3.59의 안전의식수준을 나타내고 있다. 안전의식수준은 소방공무원 > 소방업체종사자 > 안전관련 공무원 순으로 조사되었다. 조사결과에 의하면 안전에 대한 인식의 차이를 줄이기 위한 노력이 필요하다고 할 수 있다.

• 한국항공운항학회지
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• 제26권2호
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• pp.8-30
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• 2018

The purpose of this study was to investigate the relationship between empowering leadership, directive leadership, safety communication, and safety behavior. Also, we examined whether safety motivation had a moderated mediating effect on this relationship. We collected the surveys from 343 combat pilots of the Republic of Korea Air Force for the analysis. Our structural equation modeling analysis revealed that there was a positive relationship between empowering leadership and safety behavior, and safety communication fully mediated the relationship. However, there is no relationship between directive leadership and safety behavior. Also, our results showed that safety motivation moderated the relationship between empowering leadership and safety communication, but not the relationship between directive leadership and safety communication. Furthermore, the relationship between empowering leadership and safety behavior through safety communication varied significantly with the degree of safety motivation, such that the higher the safety motivation, the higher the impact of empowering leadership on safety behavior through safety communication. Finally, we discussed the implications of these results, study limitations, and practical suggestions for future research.