• Journal of Pharmaceutical Investigation
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• 제31권1호
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• pp.27-35
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• 2001

To evaluate the site-specific permeation of aspalatone (acetylsalicylic acid maltol ester, AM) through gastrointestinal tract, the enzymatic degradation and permeation studies were carried out using gastric, duodenal and jejunal mucosae of rabbits. It was found that $15.2{\pm}11.4%$, $11.6{\pm}5.2$ and $0.8{\pm}0.6%$ of the donor dose of AM, salicylmaltol (SM) and aspirin (ASA) permeated through the upper gastric mucosa after 8 hr of permeation, respectively. After 8 hr of AM permeation, SM and ASA were measured to be $15.0{\pm}1.7$ and $2.6{\pm}0.8%$ of the dose in the donor solutions, respectively, and salicylic acid (SA) was not detected even after 6 hr, suggesting a very low gastric damage. For the gastric mucosa, the increase of donor dose from 100 to $1,000\;{\mu}g/ml$ increased the permeation flux dose-dependently (r=0.9905). For the duodenal and jejunal mucosae, however, AM was fully degraded into SM and SA due to the esterase activities within 30 min. AM and ASA were not detected in the receptor solution. This result indicates that AM is not a prodrug of ASA. Addition of potassium fluoride (0.5%) into the donor solution delayed the degradation of AM, but did not allow the permeation through duodenal mucosa even by the inhibition of esterase activity. The addition of $dimethyl-{\beta}-cyclodextrin$ and $2-hydroxypropyl-{\beta}-cyclodextrin$ (5%) into the donor solutions also did not show favorable effects on the permeation of AM through various mucosae. In comparison of permeation rates of AM and ASA through the upper gastric mucosa, the flux of ASA was 4.2 times faster than AM based on the molar concentration. ASA also was fully degraded in the donor solutions faced with duodenal and jejunal mucosae within 2 hr, and was not detected in the receptor solution, suggesting a slower metabolism compared with AM.

• Molecules and Cells
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• 제24권2호
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• pp.232-239
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• 2007

$HrpN_{EP}$, from the gram-negative pathogen, Erwinia pyrifoliae, is a member of the harpin group of proteins, inducing pathogen resistance and hypersensitive cell death in plants. When the $hrpN_{EP}$ gene driven by the OsCc1 promoter was introduced into tobacco plants via Agrobacterium-mediated transformation, their resistance to the necrotrophic fungal pathogen, Botrytis cinerea, increased. Resistance to B. cinerea was correlated with enhanced induction of SA-dependent genes such as PR-1a, PR2, PR3 and Chia5, of JA-dependent genes such as PR-1b, and of genes related to ethylene production, such as NT-EFE26, NT-1A1C, DS321, NT-ACS1 and NT-ACS2. However the expression of NPR1, which is thought to be essential for multiple-resistance, did not increase. Since the pattern of expression of defense-related genes in $hrpN_{EP}$-expressing tobacco differed from that in plants expressing $hpaG_{Xoo}$ from Xanthomonas oryzae pv. Oryzae, these results suggest that different harpins can affect the expression of different defense-related genes, as well as resistance to different plant pathogens.

• 보건교육건강증진학회지
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• 제31권5호
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• pp.109-120
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• 2014

Objectives: This study was to examine the associations between obesity level and major chronic diseases in older population in Korea, using different obesity indicators. Methods: Data was from the 2008 KloSA Biomarker pilot, a nationwide sample of 514 non-institutionalized subjects (age $63.6{\pm}9.8$ years; women 57.2%). Anthropometric information was collected by home visiting nurses. Portable bioimpedence devices(Omran HBF359) were used for measuring fat mass. Obesity cut-offs used the Asian criteria of $BMI{\geq}25$, $WC{\geq}90/85cm$, and $fat%{\geq}25/35$ for men and women. Chronic disease prevalence was defined by physician-diagnosed history of 8 specified diseases. Results: Prevalence of chronic diseases significantly increased with increment of obesity level by BMI, WC and fat %. Odds ratios of acquiring chronic diseases remained significant in the obese group (BMI OR 2.76, 95%CI 1.82-4.19; WC OR 2.73, 95%CI 1.81-4.11; Fat OR 1.87 95%CI 1.26-2.78), after adjusting for age, sex, marital status, education, work participation, household income, smoking, drinking, and exercise. Conclusions: Obesity measured by all three indicators, BMI, WC, and fat mass cannot be disregarded, accentuating the significant effect on increases in disease risks among older population. BMI and WC measure appeared better to assess the risks of chronic diseases.

• 동의생리병리학회지
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• 제32권6호
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• pp.411-417
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• 2018

Last year, the elderly population aged 65 years and over exceeded 14.2 percent of the total population, and Korea has entered the ageing society. At the same time, social interest in improving the health level and quality of life in the elderly has also increased. In particular, pain is one of the most common diseases experienced by middle-aged and elderly people and is one of the factors that closely affect the quality of life. The purpose of this study was to analyze the factors affecting the pain level and pain region of Korean medicine users and the use of Korean medicine in the Korean middle aged and elderly using the 6th Korean Longitudinal Study of Ageing (KLoSA) panel. As a result, the main influencing factors of Korean medical care were subjective factors such as gender, subjective health status, and education level. In particular, half of the middle-aged and elderly who used Korean medicine experienced back and knee pain, and generally complained of low level of pain. This study suggests the need for research and development of Korean medicine that can control and relieve the pain of the middle-aged and elderly.

• 대한토목학회논문집
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• 제26권2B호
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• pp.145-152
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• 2006

본 연구에서는 영산강 유역의 본류를 대표하는 나주지점과 황룡강 유역을 대표하는 선암지점에 대하여 물리적인 매개변수를 이용하지 않는 인공신경망 이론을 이용하여 강우-유출 과정의 비선형 모형을 개발하였다. 본 연구결과 나주지점에서는 ANN_NJ_9 모형이 선암지점에서는 ANN_SA_9 모형이 강우-유출 특성을 가장 잘 반영하였다. 또한, 본 연구에서 개발한 GUI_FFS에 대하여 기 확보된 강우 및 유출량을 적용한 결과 실측치와 예측치 간에 0.98이상의 $R^2$값을 보임으로서 향후 수자원 및 하천계획 수립과 그에 따른 운영 및 관리에 효율성을 더할 수 있을 것이라 판단된다.

• Parasites, Hosts and Diseases
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• 제62권1호
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• pp.53-63
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• 2024

The intracellular parasite Babesia microti is among the most significant species causing human babesiosis and is an emerging threat to human health worldwide. Unravelling the pathogenic molecular mechanisms of babesiosis is crucial in developing new diagnostic and preventive methods. This study assessed how priming with B. microti surface antigen 1 (BHSA 1) and seroreactive antigen 5-1-1 (BHSA 5-1-1) mediate protection against B. microti infection. The results showed that 500 ㎍/ml rBMSA1 and rBMSA5-1-1 partially inhibited the invasion of B. microti in vitro by 42.0±3.0%, and 48.0±2.1%, respectively. Blood smears revealed that peak infection at 7 days post-infection (dpi) was 19.6%, 24.7%, and 46.7% in the rBMSA1, rBmSA5-1-1, compared to the control groups (healthy mice infected with B. microti only), respectively. Routine blood tests showed higher white blood cell, red blood cell counts, and haemoglobin levels in the 2 groups (BMSA1 and BMSA5 5-1-1) than in the infection control group at 0-28 dpi. Moreover, the 2 groups had higher serum interferon-γ, tumor necrosis factor-α and Interleukin-17A levels, and lower IL-10 levels than the infection control group throughout the study. These 2 potential vaccine candidate proteins partially inhibit in vitro and in vivo B. microti infection and enhance host immunological response against B. microti infection.

• 한국식품과학회지
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• 제28권6호
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• pp.1059-1064
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• 1996

키토산의 in vitro 돌연변이 억제활성을 Salmonella typhimurium reversion assay와 SOS chromotest 이용하여 살펴보았다. S. typhimurium에 의한 시험된 간접변이원 Trp-P-2에 대해서 0.1-1.0 mg/plate의 chitosan농도로 시험하였을 때, 24-65%의 돌연변이 억제활성을 나타내었다(p<0.01). Chitosan농도 0.1-0.5 mg/plate 범위에서는 용량-반응(dose-responese)관계를 나타내면서 저해효과를 나타내었으며, 0.5 mg/plate이상의 농도에서는 오히려 저해효과가 감소하는 경향이었다. 반면, 직접변이원인 SA 및 2-NF로 유도된 돌연변이에 대해서는 시험한 어느 농도에서는 chitosan에 의한 돌연변이 억제효과가 나타나지 않았다. Chitosan은 직접변이원인 4-NQO에 의한 SOS 유도에 대해 chitosan농도가 0.15 mg/assay 및 0.20 mg/assay일 때 4-NQO에 의해 유도된 유도지수(induction factor) 8.290을 4.226및 4.516으로 낮추어 46-49%의 저해활성을 나타내었다. 간접변이원인 Trp-P-2에 의한 SOS 유도에 대한 chitiosan의 억제효과는 시험한 chitosan의 농도범위에서 약 9-39%의 저해활성을 나타내었으며, chitosan농도가 0.1 mg/assay이 경우를 제외하고는 chitosan농도 증가에 따라 비례적으로 SOS유도 저해활성이 나타났다. Trp-P-2d 의해 DNA 손상을 유도한 다음 chitosan의 세포내 역제(bio-antimutagenicity) 활성을 살펴 본 결과, 저농도에서는 세포의 억제(desmutagenicity) 특성을, 0.75-1.0 mg/plate의 비교적 고농도에서는 세포내 억제특성을 나타내었다.

• 대한간호학회지
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• 제36권6호
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• pp.897-904
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• 2006

Purpose: The purpose of this study was to identify the effects of Yakson therapy as a pain management tool oil the physiologic and behavioral reponses of infants with a painful heelstick procedure. Method: Infants were randomly assigned to a group that underwent a series of Yakson therapy and a control that received nothing before a heelstick. Heart rate, oxygen saturation, and NIPS were compared between the experimental (n=16) and control (n=16) infants during an undisturbed baseline and after a standard heelstick procedure Yakson therapy consisted of laying a hand on the back, and caressing the abdomen by hand for 5 minutes. Result: The pain scores of the Yakson group were lower than the control group. Foroxygen saturation, there were statistically significant differences between groups. For heart rate, there were no statistically significant differences between groups. Conclusion: This data suggests that Yakson therapy had a pain relief effect in behavior responses and $SaO_2$. Accordingly, Yakson therapy should be used as a nursing intervention for simple pain management for a heel prick.

• 지역사회간호학회지
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• 제21권4호
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• pp.429-438
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• 2010

Purpose: This study was descriptive survey research to examine the factors affecting menopause, depression and menopausal symptoms, and the relations among these factors in South Korean midlife women. Methods: The subjects were 319 midlife women in Daegu City. Measures were Zung's Self-Report Depression Scale (1979) to check depression, and the Midlife Women's Symptom Index developed by Im (2006) to check menopausal symptoms. Data were collected from June 1 to July 31, 2010. Collected data were analyzed by mean, standard deviation, and Pearson's correlation coefficient through SPSS/WIN 18.0. Results: There was a statistically significant correlation between depression and menopausal symptoms (r=.692, p<.001). The factors affecting depression were age (r=.194, p<.001), income (r=-.307, p<.001), exercise (r=-.602, p<.001), and menopausal status (r=.224, p<.001). The factors affecting menopausal symptoms were exercise (r=-.158, p<.037), menstrual pain (r=-.171, p = .004) and menopausal status (r=.222, p<.001). Conclusion: Intervention programs for decreasing menopausal symptoms in midlife women should be focused on relieving menopausal pain and encouraging excise from youth. Further studies will be needed to examine the relations among other factors including living style, area and menopausal symptoms.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2003년도 추계학술대회 논문집
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• pp.187-191
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• 2003

The motion and time-mean drift force of a 2-D floating BBDB in waves are studied with and without taking account of fluctuating air pressure in the air chamber. It has been found numerically that the drift for a of the BBDB is in the reverse direction of propagation of the incident waves over specific frequency ranges as found by McCormick through his experiment work. The drift force is calculated by Pinkster's near-field method. Since Maruo's formula method for the drift force is always positive, Maruo's formula is only approximate and should be replaced by the correct near-field method.