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Agrobacterium-Mediated Genetic Transformation of Pepper for the Development of Blight Resistant Cultivar (고추의 역병 저항성 품종 개발을 위하여 Agrobacterium tumefaciens를 이용한 elicitin 유전자 도입)

  • Kwon, Tae-Ryong;Lee, Moon-Jung;Han, Jung-Sul;Shin, Dong-Hyun;Oh, Jung-Youl;Kim, Kyung-Min;Kim, Chang-Kil
    • Journal of Plant Biotechnology
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    • v.34 no.1
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    • pp.55-59
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    • 2007
  • The study was carried out to develop transformants resisting to Phyophthora blight disease in the domestic pepper cultivar Subicho. In transforming of syn600 promoter with elicitin gene using Agrobacterium (LBA4404/pBI101 syn600-syn${\alpha}$-elicitin) to cotyledons of pepper, rate of shoot formation in 'Subicho' was 11.1% in medium containing 3 mg/L zeatin and 0.05 mg/L NAA, and also 12.8% in medium containing combination of 4 mg/L zeatin and 0.05 mg/L MAA. For PCR reaction using elicitin gene primer of transformants regenerated from cotyledons, we detected a specific band of 536 bp, and also showed strong signal at position of 536 bp in accordance with NPTII gene used as probe in Southern blot. Transformants pepper shown resistance to blight fungus was inoculated to seedlings of the $T_{1}\;and\;T_{2}$ transformants by concentration (density: zoo spore $10^{3}/mL$).

Genetic Improvement for Yield and Yield Related Traits by Introgressive Hybridization in Sweet Corn

  • Nigussie Mandefro;Saleh Ghizan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.2
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    • pp.91-96
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    • 2005
  • Proper choice of source populations contributes to the ultimate success of selection for genetic improvement. The source population should possess the most desirable alleles at as many loci as possible for intra population improvement. Such desirable alleles can be intensified by introgression of exotic germ plasm into locally adapted ones through hybridization followed by selection. The objectives of this study were to determine the mean performance, genetic variability $({\sigma}^2G)$ and heritability of fresh ear yield and other important traits within two sweet corn source populations, $BC1-10{\times}Syn-II$ and BC2-10. One hundred selfed progenies from each of the two source populations were evaluated in a $10\times10$ lattice design, at the Institute of Bioscience (IBS) Farm, University of Putra Malaysia (UPM) following the recommended cultural practices. Significant differences among selfed progenies within $BC1-10{\times}Syn-II$ were observed for all traits, while differences among selfed progenies within BC2-10 were noted for fresh ear yield, ear length, ear diameter, number of kernels per row, ear height, days to tasseling and days to silking. Progenies developed from $BC1-10{\times}Syn-II$ population had higher estimates of ${\sigma}^2G$ than did progenies from BC2-10 population for number of kernel rows per ear, total soluble solids, plant height, days to tasseling and days to silking, showing that selection to improve these traits would be more effective in selfed progenies of $BC1-10{\times}Syn-II$ than that in BC2-10. On the other hand, progenies developed from BC2-10 population had higher estimates of ${\sigma}^2G$ for ear length, ear diameter and ear height, indicating that progenies from this population would have better genetic gain than $BC1-10{\times}Syn-II$. Comparable estimates of genetic variance were found for fresh ear yield, and number of kernels per row, indicating that genetic improvement of the two source populations is expected to produce similar genetic gains for these two traits. Therefore, selfed progenies developed from both source populations could be used to improve the two populations for various traits and thereby develop superior genotypes for immediate use in the production system.

Bench Scale Performance Test of WGS and $CO_2$ PSA for $H_2$ Recovery from Syn Gas (합성가스로부터 수소회수를 위한 WGS 및 $CO_2$ PSA 성능평가)

  • Jeong, Seong-Jae;Cha, Jae-Jun;Kim, Young-Suk
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.06a
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    • pp.206.1-206.1
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    • 2010
  • 폐기물 등을 열분해 가스화한 합성가스로부터 효과적으로 고순도의 수소를 회수하기 위하여 WGS(수성가스전환반응) 및 $CO_2$ 회수 PSA 공정을 적용하였다. 벤치스케일 열교환형 WGS반응기를 개발하여 기존 단열방식에 비하여 단순화한 반응시스템을 구축하였으며 출구 CO농도 4%대를 달성하였다. 또한 3베드로 구성된 벤치스케일의 $CO_2$ PSA운전을 수행한 결과, 2.5barg 흡착 및 진공재생단계를 적용하여 회수되는 $CO_2$의 농도가 95%이상, 회수율 80%이상을 기록하는 효율적인 $CO_2$ 회수공정을 개발하였다. 한편, 흡탈착 모사프로그램인 ADSIM을 통해서도 실험과 비교적 일치한 결과를 얻을 수 있었는데 향후 스케일업 설계자료 확보시 유용할 것으로 판단되었다.

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Studies on the Quality Reinforcement for Pneumatic Tire and Tube. Part 2. Physical Properties of NR-Syn. R blends. (Tire 및 Tube의 품질(品質) 보강(補强)에 관(關)한 연구(硏究)(제2보(第2報)) 천연(天然) 및 합성(合成)고무의 변량혼합(變量混合)에 따른 이화학적(理化學的) 성능(性能))

  • Kim, Joon-Soo;Lee, Myung-Whan;Yum, Hong-Chan;Lee, Chin-Bum;Park, Chang-Ho;Hong, Chong-Myung;Im, Dong-Ho;Lee, Chong-Koun
    • Elastomers and Composites
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    • v.3 no.1
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    • pp.32-38
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    • 1968
  • The physical properties of NR-SBR and NR-BR blends were studied. 1. In blending, tensile strength decreased with increase in synthetic rubber contents. It was most effective when the ratio of NR/Syn. R is 75/25. 2. Tensile strength decreased with order of NR, SBR and BR and modulus after aging is proportional of synthetic rubber contents. 3. Elongation is less influenced by synthetic rubber ratio that on the other hand, the decrease of elongation is proportional to synthetic rubber contents after aging. 4. Hardness decreased with increase in the synthetic rubber contents and on the other hand, the hardness increased after aging.

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