• The Korean Journal of Nuclear Medicine Technology
• /
• v.14 no.2
• /
• pp.77-82
• /
• 2010

Purpose: As PET/CT come into wide use, it caused increasing of expose in clinical use. Therefore, Korea Food and Drug Administration issued Patient DRL (Diagnostic Reference Level) in CT scan. In this study, to build the basis of patient dose reduction, we analyzed effective dose in transmission scan with CT scan. Materials and Methods: From February, 2010 to March 180 patients (age: $55{\pm}16$, weight: $61.0{\pm}10.4$ kg) who examined $^{18}F$-FDG PET/CT in Asan Medical Center. Biograph Truepoint 40 (SIEMENS, GERMANY), Biograph Sensation 16 (SIEMENS, GERMANY) and Discovery STe8 (GE healthcare, USA) were used in this study. Per each male and female average of 30 patients doses were analyzed by one. Automatic exposure control system for controlling the dose can affect the largest by a patient's body weight less than 50 kg, 50-60 kg less, 60 kg more than the average of the three groups were divided doses. We compared that measured value of CT-expo v1.7 and ImPACT v1.0. The relationship between body weight and the effective dose were analyzed. Results: When using CT-Expo V1.7, effective dose with BIO40, BIO16 and DSTe8 respectably were $6.46{\pm}1.18$ mSv, $9.36{\pm}1.96 $mSv and $9.36{\pm}1.96$ mSv for 30 male patients respectably $6.29{\pm}0.97$ mSv, $10.02{\pm}2.42$ mSv and $9.05{\pm}2.27$ mSv for 30 female patients respectably. When using ImPACT v1.0, effective dose with BIO40, BIO16 and DSTe8 respectably were $6.54{\pm}1.21$ mSv, $8.36{\pm}1.69$ mSv and $9.74{\pm}2.55$Sv for 30 male patients respectably $5.87{\pm}1.09$ mSv, $8.43{\pm}1.89$ mSv and $9.19{\pm}2.29$ mSv for female patients respectably. When divided three groups which were under 50 kg, 50~60 kg and over 60 kg respectably were 6.27 mSv, 7.67 mSv and 9.33 mSv respectably using CT-Expo V1.7, 5.62 mSv, 7.22 mSv and 8.91 mSv respectably using ImPACT v1.0. Weight and the effective dose coefficient analysis showed a very strong positive correlation(r=743, r=0.693). Conclusion: Using such a dose evaluation programs, easier to predict and evaluate the effective dose possible without performing phantom study and such dose evaluation programs could be used to collect basic data for CT dose management.

• BMB Reports
• /
• v.28 no.6
• /
• pp.538-545
• /
• 1995

When DNA replication of simian virus 40 (SV40) is initiated on the replication origin, the regions containing the initiation sites of DNA primase, which participates in the transient RNA primer synthesis for formation of Okazaki fragments in the lagging strand, were chosen as the target sites of antisense RNA for studies of the inhibition of SV40 DNA replication. Four recombinant transcription vectors, pUC-PrI, pUC-PrII, pGEM-PrBS, and pGEM-PrSN, coding antisense RNA, were constructed. Four antisense RNAs (named as I, II, BS, and SN) having the size of 18, 19,58, and 123 nts, respectively, were made from the transcription vectors by in vitro transcription. And then, antisense RNA in the concentration of 2${\mu}m$ were added to COS cells transfected with pATSV-W which is a recombinant plasmid containing the SV40 origin of replication. The inhibitory extent of DNA replication was measured by DpnI resistance and was confirmed by measurement of transient RNA primer synthesis. The result shows that six combinations of antisense RNA (I, II, BS, SN, I+SN, and BS+SN) lead to the inhibition of SV40 DNA replication by up to 85%.

• Korean Journal of Microbiology
• /
• v.26 no.2
• /
• pp.106-112
• /
• 1988

We have examined the structures and cellular distributions of the SV40 late RNAs present in monkey cells at late times after infection. One particular RNA species, spliced at residue 373(373-RNA), was found to be as abundant as the major late 16S RNAs. This result was unexpected since previous reports showed that the molecular ratio of the 373-spliced 19S RNA to 16S RNA is approximately 0.1 among either cytoplasmic polyadenylated or polysomal viral RNAs. Both sedimentation and electrophoretic analysis indicated that the 373-RNA was approximately 16S to 19S in size. Therefore, it was not a splicing intermediate or the product of premature termination of transcription within the late leader region. Whereas most SV40 late 16S RNA is polyadenylated and located in the cytoplasm, the majority of 373-RNA was found to lack poly A, and be located in the nucleus.

• Korean Journal of Microbiology
• /
• v.27 no.1
• /
• pp.1-9
• /
• 1989

The locations of 5' ends as well as the splicing pattern of viral poly A(-) 19S RNA from monkey cells infected with SV40 were determined by a modification of primer extension method. The 5' end of this RNA mapped at the major cap site at nucleotide residue 325, used most frequently by SV40 late RNAs. The intron from nt.373 to nt.558 was removed as the ordinary cytoplasmic poly A(+) 19S RNA. The 3'end of this RNA was very heterogeneous and distributed over 1 kb upstream of polyadenylation site, as determined by S1 nuclease mapping. The reason for this normally initiated and spliced RNA to accumulate in the nucleus was investigated. In order to test whether the presence of unused 3' splice region on this RNA caused such subcellular distribution, cells were transfected with SV40 mutant KNA containing deletion around 3' splice site. The RNA deleted of 3' splice region accumulated mainly in the cytoplasm. This accumulation did not result from the increased stability of the RNA due to the deletion, since the wild type and mutant RNAs exhibited similar half lives after chase with actinomycin D. Therefore it is likely that the 19S spliced RNA is hindered from being transported into the cytoplasm due to some pre-splicing complexes formed at the unused 3' splice site.

• Journal of the Korean Society of Radiology
• /
• v.5 no.4
• /
• pp.195-200
• /
• 2011

The purpose of this study is to investigate the dose of radiation exposure to radiation-related workers in a hospital setting, thus increasing awareness of the health risk to the radiation-related workers. The result of the analysis showed the average dose of radiation exposure to radiation-related workers in hospital K was $0.75{\pm}0.26mSv$ in 2008, $0.67{\pm}0.30mSv$ in 2009, and $0.92{\pm}0.33mSv$ in 2010. The average dose of radiation exposure in hospital P was $0.43{\pm}0.13mSv$ in 2008, $0.43{\pm}0.20mSv$ in 2009, and $0.33{\pm}0.85mSv$ in 2010. The average dose of radiation exposure in hospital K by age group was 13.39mSv for age 20 to 29, 8.37mSv for age 30 to 39, 1.19mSv for age 40 to 49, 0.28mSv for age 50 to 59, and 0.32mSv for age 60 to 69 The average dose of radiation exposure in hospital P by age group was 0.33mSv for age 20 to 29, 1.41mSv for age 30 to 39, 0.83mSv for age 40 to 49, 1.66mSv for age 50 to 59, and 1.12mSv for age 60 to 69. Moreover, the average radiation exposure to radiation-related workers over 3 year period by gender group in hospital K was $2.92{\pm}1.03mSv$ for male group and $0.94{\pm}0.93mSv$ for female group. The average radiation exposure over 3 year period by gender group in hospital P was $0.66{\pm}0.18mSv$ for male group and $1.80{\pm}0.60mSv$ for female group. Persons working in diagnostic radiology department received mean of $1.65{\pm}1.54mSv/year$, mean $1.17{\pm}0.82mSv/year$ in radiation oncology, mean $1.79{\pm}1.42mSv/year$ at nuclear medicine department and mean $0.99{\pm}0.51mSv/year$ at other departments. Radiation exposure was higher than that of other departments(p<0.05). Doctors and technologists received higher radiation exposure (mean $1.75{\pm}1.17mSv/year$, $1.60{\pm}1.39mSv/year$ each) than other workers(p<0.05). Measurement and evaluation of radiation exposure in radiation-related workers should be widely conducted accurately and consistently in the radiation-related occupational setting so that people in these occupational settings are more aware of the risk from radiation exposure, and thus give more attention and caution to decrease radiation exposure. It would be essential to minimize accumulated radiation dose in the radiation-related occupational setting in order to maintain and improve the health of radiation-related workers.

• Biomedical Science Letters
• /
• v.10 no.2
• /
• pp.85-92
• /
• 2004

Immortalization of primary corneal cells has influence on pharmacy, medical and biological fields. Especially, investigation of immortalization mechanism using viral oncoproteins is useful for medical treatments, and these cell lines will be useful materials for toxic test of medical supplies and cell biological experiments. Rabbit corneal fibroblasts in culture undergo a finite number of divisions before they reach a terminally non-proliferating state known as replicative senescence. Therefore, we attempted to induce immortalization of rabbit corneal fibroblasts with SV 40 large T antigen. As a result of experiment, expression of SV 40 large T antigen was confirmed, and expression of proteins related to cell cycle repressor was decreased in the transfection group compared with non-transfection group. According to the results of cell cycle phase distribution test, SV 40 large T antigen-transfected cells had obtained higher proliferation rate than primary cells. It was confirmed that during induction of immortalization, SV 40 large T antigen was not able to increase telomerase activity. In conclusion, we made a rabbit corneal fibroblast cell line with SV40 large T antigen. This cell line will be useful for further studies of mammalian fibroblast biology, particularly with regard to angiogenesis and malignant transformation. In addition, this cell line offers opportunity for testing potential therapeutics and can be used for toxicity tests of materials or cosmetics. In the future, our cell line can potentially be utilized in a wide range of biology related fields.

• Asian-Australasian Journal of Animal Sciences
• /
• v.8 no.5
• /
• pp.449-454
• /
• 1995

The present study was conducted to assess gene expression of bacterial lacZ driven by the SV40 promoter at early developmental stages of bovine embryos. The lacZ gene was linearized with BamHI digestion and introduced into the pronucleus by microinjection at 20 hrs after the commencement of in vitro fertilization. Intact bovine blastocysts were not stained with X-Gal, suggesting that there is no endogenous beta-galactosidase activity in these blastocysts. In contrast, the bovine blastocyst cells microinjected with the lacZ gene exerted a characteristic greenish-blue color originating from the bacterial beta-galactosidase activity, albeit at a low rate, i.e. 2.1% of the total fertilized oocytes injected. It was concluded, therefore, that the lacZ gene driven by the SV40 promoter could be used for an indirect screening method in which the presence of transgene is evaluated from the product of transgene expression.

• Journal of radiological science and technology
• /
• v.38 no.1
• /
• pp.23-30
• /
• 2015

Positron emission tomography scan has been growing diagnostic equipment in the development of medical imaging system. Compare to 99mTc emitting 140 keV, Positron emission radionuclide emits 511 keV gamma rays. Because of this high energy, it needs to reduce radioactive emitting from patients for radio technologist. We searched the external dose rates by changing distance from patients and measure the external dose rates when we used shielder investigate change external dose rates. In this study, the external dose distribution were analyzed in order to help managing radiation protection of radio technologists. Ten patients were searched (mean age: $47.7{\pm}6.6$, mean height: $165.5{\pm}3.8cm$, mean weight: $65.9{\pm}1.4kg$). Radiation was measured on the location of head, chest, abdomen, knees and toes at the distance of 10, 50, 100, 150, and 200 cm, respectively. Then, all the procedure was given with a portable radiation shielding on the location of head, chest, and abdomen at the distance of 100, 150, and 200 cm and transmittance was calculated. In 10 cm, head ($105.40{\mu}Sv/h$) was the highest and foot($15.85{\mu}Sv/h$) was the lowest. In 200 cm, head, chest, and abdomen showed similar. On head, the measured dose rates were $9.56{\mu}Sv/h$, $5.23{\mu}Sv/h$, and $3.40{\mu}Sv/h$ in 100, 150, and 200 cm, respectively. When using shielder, it shows $2.24{\mu}Sv/h$, $1.67{\mu}Sv/h$, and $1.27{\mu}Sv/h$ in 100, 150, and 200 cm on head. On chest, the measured dose rates were $8.54{\mu}Sv/h$, $4.90{\mu}Sv/h$, $3.44{\mu}Sv/h$ in 100, 150, and 200 cm, respectively. When using shielder, it shows $2.27{\mu}Sv/h$, $1.34{\mu}Sv/h$, and $1.13{\mu}Sv/h$ in 100, 150, and 200 cm on chest. On abdomen, the measured dose rates were $9.83{\mu}Sv/h$, $5.15{\mu}Sv/h$, and $3.18{\mu}Sv/h$ in 100, 150, and 200 cm, respectively. When using shielder, it shows $2.60{\mu}Sv/h$, $1.75{\mu}Sv/h$, and $1.23{\mu}Sv/h$ in 100, 150, and 200 cm on abdomen. Transmittance was increased as the distance was expanded. As the distance was further, the radiation dose were reduced. When using shielder, the dose were reduced as one-forth of without shielder. The Radio technologists are exposed of radioactivity and there were limitations on reducing the distance with Therefore, the proper shielding will be able to decrease radiation dose to the technologists.

• Korean Journal of Environmental Agriculture
• /
• v.23 no.1
• /
• pp.52-58
• /
• 2004

This work was conducted to provide the reference data of radioactivity in the foodstuffs at a radiological emergency situation in Jeju Island The sampled foodstuffs were agricultural (31), livestock (6), marine (12) and forest products (4), and processed foods (3) consumed by Jeju Islanders. $^{137}Cs$ and $^{40}K$ activities were determined by HPGe r-ray spectromety. The activity ranges of $^{137}Cs$ was ${\sim}650\;mBq/kg$ fresh in the agricultural products, ${\sim}131\;mBq/kg$. fresh in the livestock, ${\sim}834\;mBq/kg$ fresh in the forest, ${\sim}253\;mBq/kg$ fresh in the marine and $32.0{\sim}483\;mBq/kg$. fresh in the processed foods (tea). In case of $^{40}K$ the activity was $16.6{\sim}542\;Bq/kg$. fresh in the agricultural products, $39.1{\sim}294\;Bq/kg$ fresh in the livestock, $85.5{\sim}116\;Bq/kg$ fresh in the forest, $50.1{\sim}657\;Bq/kg$ fresh in the marine, and $33.6{\sim}1,065\;Bq/kg$ fresh in the processed foods (tea). The highest activity of $^{137}Cs$, 834mBq/kg fresh was observed in oak mushroom and $^{40}K$ 1,065 Bq/kg fresh in coffee. Annual effective doses of $^{137}Cs$ and $^{40}K$ by intake of foodstuffs per capita were the following order; agricultural products (66,543 nSv) > livestock products (19,311 nSv) > processed foods (6,648 nSv) > marine products (6,579 nSv) > forest products (860 nSv). Therefore, total annual effective dose was summed 99,941 nSv which is quite low level comparing to the annual effective dose by external exposure, 2,400,000 nSv. The data obtained in this study can be useful for monitoring whether the foodstuffs are contaminated or not at an emergency radiation accident, and showed that the foodstuffs consumed in Jeju are safe in terms of annual effective dose of $^{137}Cs$ and $^{40}K$

• Journal of the Korean Society of Radiology
• /
• v.15 no.2
• /
• pp.109-115
• /
• 2021

This study is a study on the difference in dose according to the presence or absence of gonadal shielding of scattered rays generated during chest computed tomography examination, and the scattered dose of the examination site was measured by placing the RadEye G-10 device in the center of the phantom. When the gonads are not shielded, the scattering lines of the whole, both sides, posterior and gonads are measured and Xenolite nolead Apron (0.35 mm PB), Xenolite nolead Apron (front 0.35 mm PB Mix back 0.25 mm PB, Skirt overlap), Half Apron After shielding with (0.5 mm PB), each scattered dose was measured. During chest computed tomography, the scattered dose of the test site was measured at 272 μSv, and when not shielded with Apron, the average total was 43 μSv, left 81 μSv, right part 82 μSv, posterior part 38.8 μSv, and Gonad part 16 μSv. Became. Xenolite nolead Apron shielded only the upper part and measured all 11.2 μSv, left part 43.1 μSv, right part 45.3 μSv, posterior part 12 μSv and Gonad part 5.2 μSv. Xenolite nolead Apron (Skirt overlap) covered the Pelvis area 360° and the dose was measured to be 5.6 μSv in the whole, 22.4 μSv in the left, 15.7 μSv in the right side, 6 μSv in the posterior part, and 3.2 μSv in the Gonad part. Xenolite nolead Apron (Skirt overlap) covered the Pelvis area 360° and the dose was measured to be 5.6 μSv in the whole, 22.4 μSv in the left, 15.7 μSv in the right side, 6 μSv in the posterior part, and 3.2 μSv in the Gonad part. When measuring only the upper part with Half Apron, the total measurement was 10.7 μSv, the left part 42.6 μSv, the right part 40.6 μSv, the posterior part 11.3 μSv, and the Gonad part 4.7 μSv. The method of 360° shielding of the pelvic area showed a dose reduction of more than 80%, and a dose reduction effect of more than 70% was shown when all shielding was performed. In all computerized tomography examinations, research to reduce the exposure dose and various shielding devices were used. It is believed that continuous research on the technique is needed.