• The Korea Journal of Herbology
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• v.24 no.1
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• pp.159-167
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• 2009

Objectives : So-Dang-Hwan (SDH) is used as a traditional treatment of diabetes in oriental clinics in Korea. This study aimed to evaluate antidiabetic effect of SDH in streptozotocin (STZ)-induced diabetic rats. Methods : Diabetes was induced by i.p. injection of STZ (45 mg/kg) to Sprague-Dawley rats. Experimental animals (eight per group), were treated by oral administration of SDH (60 mg/kg body weight) and glibenclimide (1 mg/kg), a known antidiabetic drug for comparison, during 5 weeks. To veridy the effect of SDH, the levels of glucose, triglyceride, insulin, BUN and creatinine were measured in sera from experimental diabetic rats, and an oral glucose tolerance test (OGTT) was also performed. Results : SDH prevented body weight loss in diabetic rats. SDH exhibited at termination, a significant reduction in blood glucose levels in STZ-induced diabetic rats. SDH significantly reduced serum creatinine levels toward the normal levels. The OGTT results showed a significant improvement in glucose tolerance in rats treated with SDH. Conclusions : These data indicate that SDH treatment may improve glocose homeostasis in STZ-induced diabetes.

• Journal of Acupuncture Research
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• v.22 no.5
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• pp.1-10
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• 2005

Objectives : The Present study was carried out to investigate the preventive effect of Anti-diabetic prescription 1(AD-1) on streptozotocin(STZ)-induced diabetic nephropathy. Methods : AD-1 consists of eleven herbs that have an effect on diabetes mellitus. AD-1 was given to rats with the combination of oral administration and herbal-acupuncture stimulation. The experimental animals were divided into 3 groups : normal group of rats, control group of STZ-induced diabetic rats, sample group with AD-1 treatment. Experimental diabetes was induced by the injection of STZ(60mg/kg) to e rat via the peritoneum. The effect of AD-1 on STZ-induced diabetic nephropathy was observed by measuring the serum level of creatinine and BUN. Urine secretion of albumin for 24 hours and urine level of glucose measures too. Anti-oxidative stress of AD-1 administration in living body was estimated by measuring lipid peroxide and GSH content in cortex of kidneys. Results : STZ induced increase of serum creatinine, BUN and albumin secretion were lowered by AD-1 treatment. Conclusion : The AD-1 treatment showed protective effect on rat diabetic nephropathy model, and action mechanism of the effect was thought to be concerned with anti-oxidative stress.

• The Korea Journal of Herbology
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• v.26 no.4
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• pp.23-30
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• 2011

Objectives : This study aimed to evaluate the effect of Atractylodis Rhizoma Alba water extract on streptozotocin (STZ)-induced diabetes in rats. Methods : Male Sprague-Dawley rats were divided into four groups ; normal, STZ-control and Atractylodis Rhizoma Alba (A) water extract-administrated group. Rats in which diabetic was induced by intraperitonal injection with STZ(60 mg/kg body weight). STZ-induced diabetic rats were orally administrated A extract daily for 5 weeks at doses of 200 or 500 mg/kg. Fasting blood glucose, total cholesterol, triglyceride and blood urea nitrogen were measured in sera of rats. Total volume of urine and urinary creatinine were also measured. Histopathological examination and immunohistochemical staining for the expression of insulin and ${\alpha}$-SMA in pancreas and kidney were performed, respectively. Results : There were no differences in body and kidney weights between STZ-control and A extract-administrated groups. However, serum triglyceride level was significantly decreased in A extract-administrated groups compared with those of STZ-control group. Histopathological analysis of pancreas and kidney revealed increased the number of islets and insulin-positive beta-cells in pancreas, and decreased morphological changes of glomerulus and ${\alpha}$-SMA expression in kidney after the administration of A extract. Conclusions : These results suggest that Atractylodis Rhizoma Alba has a biological action on STZ-induced diabetes in rats via decreasing the serum levels of total triglyceride, and suppressing the morphological changes of pancreas and kidney.

• The Korea Journal of Herbology
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• v.23 no.4
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• pp.103-112
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• 2008

Objectives: Indongdeungjikolpi-tang(IJT) is used as a traditional treatment of diabetes in oriental clinincs. This study aimed to evaluate the anti-diabetic effect of Indongdeungjikolpi-tang(IJT) in streptozotocin(STZ)-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of streptozotocin(STZ; 60 mg/kg BW) to Sprague-Dawley male rats. Experimental animals(six per group), were treated by oral administration of IJT(100 and 500 mg/kg BW) and glibendimide(3 mg/kg), a known antidiabetic drug for comparison, during 4 weeks. We measured the levels of glucose, insuline, triglyceride, creatinine and urea in sera of each group. An oral glucose tolerance test(OGTT) was also performed in all groups. Results: IJT(100 and 500 mg/kg) significantly reduced blood glucose levels and increased plasma insulin levels in STZ-induced diabetic rats. IJT also significantly reduced the plasma levels of tryglyceride, creatinine and urea in STZ-induced diabetic rats. The OGTT results showed a significant improvement in glucose tolerance in IJT-administrated rats. Conclusions: These data indicate that IJT may improve glocose homeostasis in STZ-induced diabetes, which could be associated with stimulation of insulin secretion.

• Biomolecules & Therapeutics
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• v.4 no.4
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• pp.437-442
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• 1996

Hypoglycemic effect of Tabebuia avellandae was investigated in the streptozotocin(STZ)-induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats by injections of STZ (45 mg/kg, i.v.). Rats weighing 200-250 g were divided into 6 groups: normal, STZ-control, hexane fr., CHCl$_3$fr., BuOH fr. and $H_2O$ fr. group. Normal and STZ-control rats received 3% Tween 80 only. Four groups of diabetic rats were administered orally at doses of 100, 400, 300 and 400 mg/kg/day of hexane, CHC1$_3$, BuOH and $H_2O$ fr. respectively. Fractions were administered orally to the rats for 7 days after STZ injection. All rats were anesthetized with ether, blood samples were taken by cardiac puncture for clinical chemistry and the rats were killed by exsanguination. Liver, kidney, heart and spleen were removed, weighed and analyzed. We measured glucose, protein, cholesterol and triglyceride levels in the plasma and glycogen, cholesterol and triglyceride levels in liver. The extent of blood glucose decrement in rats administered $H_2O$ fraction was greater than that in the STZ-control rats. The serum cholesterol and triglyceride levels were significantly lowered by administration of $H_2O$ fraction compared with those of STZ-control group. Treatment of rats with Tabebuia avellandae fractions caused decreases in STZ-induced elevation of cholesterol and triglyceride. Liver triglyceride level was significantly lowered hexane and BuOH fraction group compared with STZ-control group. These results suggest that $H_2O$ fraction of Tabebuia avellandae has the hypoglycemic action against STZ-induced diabetic rats.

• Journal of Life Science
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• v.20 no.5
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• pp.691-696
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• 2010

In this study, we investigated the antioxidative effects of So-Dang-Tang (SDT) on streptozotocin (STZ)-induced diabetic rats. Diabetes was induced by intraperitoneal injection of STZ (45 mg/kg body weight) into Sprague-Dawley rats. The SDT (200 mg/kg) and the reference drug, glibenclimide (1mg/kg), were orally administered once a day for 28 days in STZ-induced diabetic rats. The activity of antioxidant enzymes, including those of superoxide dismutase (SOD) and catalase, and the levels of glutathione (GSH) and production of malondialdehyde (MDA) were measured in the liver, kidney, and pancreas of diabetic rats. Treatment with SDT in STZ-induced diabetic rats significantly increased the activities of antioxidant enzymes and GSH levels in the liver, kidney, and pancreas when compared to those of the STZ-control group. SDT also significantly decreased lipid peroxidation product and MDA levels in STZ-induced diabetic rats. These results indicate that SDT has an antioxidative action in STZ-induced diabetic rats.

• Journal of Haehwa Medicine
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• v.29 no.1
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• pp.18-25
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• 2020

Objective: The goal of this study is to investigate whether peripheral axonal regeneration is affected by diabetes in experimental animals. Method: Sprague Dawely rat was injected with 45~50 mg/kg of streptozotocin (STZ) to generate an animal model of diabetes. Three months after STZ injection, sciatic nerve (2 cm length) was removed and the same length of nerve segments from STZ-injected animal or from control animal (CTL) was transplanted into STZ-injected animals (STZ-STZ and STZ-CTL respectively). Similarly, sciatic nerve segments from STZ-injected animal or from control animal were grafted into the control animals (CTL-STZ and CTL-CTL respectively). All animals were sacrificed 2 weeks after transplantation. Sciatic nerve sections were prepared and subjected to immunofluorescence staining analysis. Results: Immunofluorescence staining for NF-200 showed that distal elongation of regenerating axons reached 40~80% of proximal neve in both CTL-STZ and CTL-CTL groups. However, distal elongation in both STZ-STZ and STZ-CTL groups were 20~60% of proximal nerve. Furthermore, measurement of axonal regeneration after immuno-staining with SCG10 showed that the scores of distal elongation relative to proximal nerve were 50~90% in CTL-CTL and CTL-STZ groups and 10-60% in STZ-CTL and STZ-STZ. Conclusions: Our data showed that the levels of axonal regeneration were not affected irrespective of whether they were from STZ- or CTL graft, but were greatly reduced when the nerves were transplanted into the STZ host.

• Journal of the Korean Academy of Clinical Electrophysiology
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• v.2 no.2
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• pp.13-23
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• 2004

This study aimed at examining the effects of percutaneous electrical nerve stimulation(PENS) applied to different parts of the streptozotocin(STZ) induced diabetic rats on the change of glucose level and spinal neuron excitability. A total of twenty-eight SD rats, divided into four groups, were used as experiment animal. Experiment group I, the normal control group, was composed of normal rats without diabetes induction. Experiment group was composed II of the rats without any treatment after experimental diabetes induction. Experiment group III was composed of the rats with 2 Hz and $200\;{\mu}s$ of PENS to the acupuncture points related with diabetes for 20 minutes after diabetes induction. Experiment group IV was composed of the rats with 2 Hz and $200\;{\mu}s$ of PENS to the parts unrelated with diabetes for 20 minutes after diabetes induction. The results can be summarized as follow: As for glucose level, the group I showed no change within normal range, and the group III showed significant increase, compared with other groups (p<0.05). As for the change of H latency, M and H amplitude, the group III showed significant differences in decrease of latency and amplitude (p<0.05). As for Hmax/Mmax ratio, the normal and other groups showed no significant differences in decrease of amplitude. It can be concluded from the above results that PENS to the acupuncture points of the STZ-induced diabetic rats was effective for spinal neurone excitability, in particular, for those of the group with PENS to the acupuncture points. This study was conducted in the period of acute diabetes induction, and therefore, further study should be conducted in the period of chronic diabetes to research both acute and chronic diabetes.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.6
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• pp.1267-1272
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• 1998

In order to investigate the preventive and therapeutic of royal jelly on diabetes, the levels of blood glucose and serum lipids as well as the number of blood cells were determined in streptozotocin(STZ) diabetic rats. Rats were divided into seven groups. The RJ group was administered royal jelly and the STZ group was treated with streptozotocin to induce diabetes. To determine the preventive effect, diabetes was induced after administration of royal jelly for 2 weeks in group RS1/RS2. In group SR1/SR2 diabetic rats were administered royal jelly for 2 weeks to investigate the therapeutic effect. After 3 weeks, the body weight was reduced in STZ and SR1 groups and food intake was increased in the STZ, RS1 and SR1 groups. The blood glucose level was similar to the control group in the RJ, RS1 and RS2 groups and there was no effect in the other groups. The total lipid and triglyceride level were lower in the SR1 group as compared to STZ, and the total cholesterol level was higher in the STZ group. The index of atherogenesis was lower in the RJ and SR1 groups compared to the normal group. The number of red blood cells and hemoglobin was higher in the RJ and SR1 groups and the number of white blood cells was higher in the RJ and SR2 groups.

• Korean Journal of Veterinary Research
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• v.40 no.3
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• pp.489-496
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• 2000

This study was conducted to investigate the effects of streptozotocin-induced (STZ) diabetes on insulin-like growth factor-I (IGF-I), insulin-like growth factor binding proteins (IGFBPs), and IGF-I carrier proteins in serum, liver, and kidney. The levels of total and free IGF-I were measured by radioimmunoassay (RIA). The patterns of IGFBPs were determined by western ligand blotting (WLB) analysis. The profiles of IGF-I carrier proteins in serum were determined by column chromatography. The levels of total and free IGF-I in serum were lower in STZ-induced diabetic rat than normal rat (p<0.01). Similarly, the levels of total IGF-I in liver was lowered in STZ-induced diabetic rats. On the other hand, the levels of total IGF-I in kidney were increased in STZ-induced diabetic rats compared with normal rats (p<0.01). In serum and liver from STZ-induced diabetic rats, the amount of IGFBP-3 was decreased and the amount of IGFBP-2 was increased compared with normal rats. There was a not difference in amount of IGFBP-4 in serum between STZ-induced diabetic rats and normal rats. The serums of normal rats have higher 150kDa carrier proteins than in STZ-induced diabetic rats, whereas, most of 50kDa carrier proteins were found in STZ-induced diabetic rats. These results demonstrate that in STZ-induced diabetic rats, IGF-I/IGFBPs system that included functional bioactivity was changed in serum as well as tissues, and these changes may play an important role in pathogenesis of diabetes.