• BMB Reports
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• 제50권2호
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• pp.85-90
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• 2017

Recently, we demonstrated that superoxide dismutase 3 (SOD3) is a strong candidate for biomedicine. Anti-oxidant function of SOD3 was accomplished without cell penetration, and it inhibited the inflammatory responses via non-enzymatic functions. SOD3 has the heparin binding domain associating cell surface. Interestingly, we found that $Zn^{2+}$ promotes transduction effects of recombinant human SOD3 (rhSOD3) by increasing uptake via the heparin binding domain (HBD). We demonstrated an uptake of rhSOD3 from media to cell lysate via HBD, resulting in an accumulation of rhSOD3 in the nucleus, which was promoted by the presence of $Zn^{2+}$. This resulted in increased inhibitory effects of rhSOD3 on NF-{\kappa}B and STAT3 signals in the presence of $Zn^{2+}$, which shows elevated association of rhSOD3 into the cells. These results suggest that an optimized procedure can help to enhance the inflammatory efficacy of rhSOD3, as a novel biomedicine.

• Asian Journal for Public Opinion Research
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• 제2권2호
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• pp.103-120
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• 2015

Since 1795, 106 districts in Japan have been selected as Important Historic Preservation Districts (Juuyo dentouteki kenzoubutsugun hozon chiku [Juudenken]). The system for protection of cultural properties enables the local government to name a "Preservation District" and allows for the development of a preservation plan based on local ordinances. Moreover, the well-organized, bottom-up networks, which are groups for community development activities on the basis of local participation, play an important role in raising awareness and conducting several preservation projects in their own towns. This study mainly focused on cultural resources management in the local community. The system, which possibly bridged the gap between the local authorities and the community, was revealed. Fifty non-profit groups and active citizens, who were engaged in an advanced stage of community participation in Juudenken, were selected to be interviewed. The results then were analyzed using STAT program. The significant associations were shown by mapping the associations related to the public process of community involvement. Each variable had its own significant meaning and contributed credible indirect association to community involvement. The network mapping indicated that balancing the local economy and technical conservation was important in generating community involvement, which provided a model on how local authorities and communities could articulate and maintain their own cultural resources.

• 생명과학회지
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• 제22권9호
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• pp.1243-1253
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• 2012

뇌하수체 전엽에서 성장호르몬의 생산과 분비는 세포의 분열과 분화 그리고 이동을 조절하는 몇 가지 천연물질에 의해 유도된다. 따라서 발효과정을 통해 제조된 청국장이 성장호르몬의 대사에 미치는 영향을 평가하기 위하여 성장호르몬 분비능과 반응성을 뇌하수체 세포와 성장호르몬 표적세포에서 관찰하였다. 6가지 종류의 콩 품종으로 제조된 청국장 추출물 중에서, 대원, 대풍, 태광의 3종류 청국장 추출물은 5 mg/ml 농도에서 GH3 세포로부터 성장호르몬의 분비를 촉진하였다. 비록 세포 생존능은 이러한 추출물에 의해 유의적인 변화가 유도되지 않았으나, 성장호르몬의 분비량은 청국장 추출물의 농도에 의존적으로 증가하였다. 또한 성장호르몬의 표적 기관으로부터 유래된 MG63과 HepG2 세포는 GH3로부터 수집된 조건적 배양액에 의해 유의적으로 활성화되었다. 또한 이러한 세포에서 STAT5 발현은 대원 청국장 추출물을 처리 후, 15분 혹은 30분부터 세포질에서 유의적으로 증가하였으며, p-STAT5는 핵에서 30분 혹은 60분부터 증가하였다. 따라서 이러한 결과는 3가지 종류의 청국장 추출물은 성장호르몬의 분비를 촉진시키며, 청국장의 조건적 배양액은 성장호르몬 표적세포에서 신호전달을 유도함을 제시하고 있다.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2009년도 춘계 종합학술대회 논문집
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• pp.306-311
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• 2009

본 연구는 질병과 관련이 있는 단백질들은 질병네트워크를 형성함에 있어서 매우 중요한 인자로 작용할 가능성이 있다는 아이디어에서 출발한다. 우리는 Online Mendelian Inheritance in Man(OMIM)과 SWISS-PROT으로부터 인간의 단백질 데이터와 질병 정보를 확보하고 질병관련 단백질의 단백질 상호작용 네트워크를 구축 한 후, 이를 바탕으로 질병네트워크를 구축했다. 그 결과 단백질 상호작용 네트워크에는 CALM1, ACTB 및 ABL2와 같은 40개의 허브 단백질이 존재하는 것을 확인했다. 단백질 상호작용 네트워크와 질병 네트워크를 통해서 우리는 질병들간의 상관관계와 각 질병에 작용하는 단백질들의 상관관계를 파악할 수 있었다. 구축된 질병네트워크로부터 APP, ABL1 및 STAT1과 같은 38개의 질병단백체를 찾아냈다. 우리는 이전 연구에서 허브 단백질들이 서브 질병네트워크에서 질병 단백체의 경향이 있다는 것을 증명했다. 하지만, 본 연구에서 전체 질병 네트워크를 분석한 결과 전체 40개의 허브 단백질 중 단 18% 허브 단백질만이 질병단백체임이 확인되었다. 현시점에서 허브 단백질-질병단백체 경향성이 전체 질병네트워크와 서브 질병네트워크간의 차이를 설명할 수 없다. 비록 우리가 이러한 풀리지 않은 문제를 안고 있지만, 단백질-질병 네트워크의 구조 및 기능 분석은 복잡한 인간 질병 시스템에서 분자 수준의 기작과 생물학적 과정을 이해하는데 중요한 정보를 제공할 것이다.

• Animal cells and systems
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• 제2권2호
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• pp.153-164
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• 1998

Cytokines regulate proliferation, differentiation and functions of haemotopoietic cells. Each cytokine possesses a variety of activities on various target cells (pleiotropy) and various cytokines have similar and overlapping activities on the same target cells (redundancy). The nature of these cytokine activities predicts unique feature of cytokine receptors, namely, cytokine has multiple receptors, different cytokines share a common receptor, and different cytokine receptors are linked to common signaling pathways. cDNA cloning of genes for cytokine receptors revealed distinct sets of receptor family with different structural features. The cytokine receptor superfamily consists of a largest family, and contains more than twenty cytokine receptor subunits. This receptor has common structural features in both extracellular and intracellular regions without tyrosine kinase domain. Another striking feature of the receptor is to share common subunit of multiple cytokines, which partly explains the redundancy of activities of some cytokines. Recent studies revealed detailed signaling events of the cytokine receptor, the primary activation of JAK and subsequent phosphorylation of tyrosine residues of receptor, and various cellular proteins. Many SH2 containing adapter proteins play an important role in cytokine signals, and this system has similarities with tyrosine kinase receptor signal transduction. STAT may mainly account for cytokine specific functions as suggested by knockout mice studies. It is of importance to note that cytokine activates multiple signaling pathways and the balance and combination of related signaling events may determine the specificity of functions of cytokines.

• 대한두경부종양학회지
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• 제37권2호
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• pp.11-17
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• 2021

Head and neck cancer is the 6^th most frequently diagnosed solid tumor in the world. Alcohol consumption, smoking, and HPV infection are associated with the incidence of head and neck squamous cell carcinoma (HNSCC). Although a multidisciplinary approach is a key strategy for the treatment of locally advanced HNSCC, systemic therapy is the mainstream of recurrent or metastatic HNSCC treatment. Stage IV HNSCC has a relatively poor prognosis with median overall survival of around one year. There have been many clinical trials to investigate the efficacy of target agents in the treatment of HNSCC. In the HPV-negative HNSCC, TP53 and CDKN2A are the most commonly mutated genes. In the HPV-positive HNSCC, the PI3K pathway is frequently altered. EGFR, PI3K, cell cycle pathway, MET, HRAS, and IL6/JAK/STAT pathway are explored targets in HNSCC. In this study, we review the target pathways and agents under research. We also introduce here umbrella trials of recurrent or metastatic HNSCC conducted by the Korea Cancer Study Group. The combination of target agents with immune checkpoint inhibitors or cytotoxic chemotherapies would be a future step in the precision medicine of HNSCC treatment.

• Journal of Interdisciplinary Genomics
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• 제5권2호
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• pp.18-23
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• 2023

Conventional evaluation method for identifying the organic cause of short stature has a low detection rate. If an infant who is small for gestational age manifests postnatal growth deterioration, triangular face, relative macrocephaly, and protruding forehead, a genetic testing of IGF2, H19, GRB10, MEST, CDKN1, CUL7, OBSL1, and CCDC9 should be considered to determine the presence of Silver-Russell syndrome and 3-M syndrome. If a short patient with prenatal growth failure also exhibits postnatal growth failure, microcephaly, low IGF-1 levels, sensorineural deafness, or impaired intellectual development, genetic testing of IGF1 and IGFALS should be conducted. Furthermore, genetic testing of GH1, GHRHR, HESX1, SOX3, PROP1, POU1F1, and LHX3 should be considered if patients with isolated growth hormone deficiency have short stature below -3 standard deviation score, barely detectable serum growth hormone concentration, and other deficiencies of anterior pituitary hormone. In short patients with height SDS <-3 and high growth hormone levels, genetic testing should be considered to identify GHR mutations. Lastly, when severe short patients (height z score <-3) exhibit high levels of prolactin and recurrent pulmonary infection, genetic testing should be conducted to identify STAT5B mutations.

• 한국생산제조학회지
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• 제8권4호
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• pp.68-78
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• 1999

Heat transfer performance is studied for boiling and condensation of R-11 on integral-fin tubes. Nine tubes with trapezoidal integral-fins having fin densities from 748 to 1654fpm and 10,30 grooves and finned tubes with caves of 0.55 and 0.64 mm height respectively are tested. in case of condensation CFC-11 condensates at saturation stat of 32$^{\circ}C$ on the outside surface cooled by inside cooling water flows. And in case of boiling the refrigerant evaporates at a saturation state of 1 bar on the outside tube surface and heat is supplied by hot water which circulates inside of the tube,. The tube having fin transfer coefficient concerns fin tubes with caves show higher valve than low fin tube having find density of 1299fpm and 30grooves. The overall heat transfer coefficient of fin tube with caves is about 5155 W/mK at 2.8m/s of water velocity, The value is abuot 2.7 times higher than plain tube and 1.3 times higher than low fin tube having fin density of 1299fpm and 30 grooves.

• 한국생산제조학회지
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• 제8권4호
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• pp.65-65
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• 1999

Heat transfer performance is studied for boiling and condensation of R-11 on integral-fin tubes. Nine tubes with trapezoidal integral-fins having fin densities from 748 to 1654fpm and 10,30 grooves and finned tubes with caves of 0.55 and 0.64 mm height respectively are tested. in case of condensation CFC-11 condensates at saturation stat of 32℃ on the outside surface cooled by inside cooling water flows. And in case of boiling the refrigerant evaporates at a saturation state of 1 bar on the outside tube surface and heat is supplied by hot water which circulates inside of the tube,. The tube having fin transfer coefficient concerns fin tubes with caves show higher valve than low fin tube having find density of 1299fpm and 30grooves. The overall heat transfer coefficient of fin tube with caves is about 5155 W/mK at 2.8m/s of water velocity, The value is abuot 2.7 times higher than plain tube and 1.3 times higher than low fin tube having fin density of 1299fpm and 30 grooves.

• Journal of Korean Neurosurgical Society
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• 제59권2호
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• pp.154-157
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• 2016

A continuous electroencephalography (cEEG) can be helpful in detecting vasospasm and delayed cerebral ischemia in aneurysmal subarachnoid hemorrhage (SAH). We describe a patient with an aneurysmal SAH whose symptomatic vasospasm was detected promptly by using a real-time cEEG. Patient was immediately treated by intraarterial vasodilator therapy. A 50-year-old woman without any significant medical history presented with a severe bifrontal headache due to acute SAH with a ruptured aneurysm on the anterior communicating artery (Fisher grade 3). On bleed day 6, she developed a sudden onset of global aphasia and left hemiparesis preceded by cEEG changes consistent with vasospasm. A stat chemical dilator therapy was performed and she recovered without significant neurological deficits. A real-time and protocol-based cEEG can be utilized in order to avoid any delay in detection of vasospasm in aneurysmal SAH and thereby improve clinical outcomes.