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http://dx.doi.org/10.5352/JLS.2012.22.9.1243

Effects of Chungkookjang Extract on Growth Hormone Secretion from GH3 Mouse Pituitary Cell and Growth Hormone Receptor Signaling Pathway  

Choi, Sun-Il (Department of Biomaterial Science, College of Natural Resources & Life Science, Pusan National University)
Kim, Ji-Eun (Department of Biomaterial Science, College of Natural Resources & Life Science, Pusan National University)
Hwang, In-Sik (Department of Biomaterial Science, College of Natural Resources & Life Science, Pusan National University)
Lee, Hye-Ryun (Department of Biomaterial Science, College of Natural Resources & Life Science, Pusan National University)
Lee, Young-Ju (Department of Biomaterial Science, College of Natural Resources & Life Science, Pusan National University)
Son, Hong-Joo (Department of Life Science and Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University)
Kim, Dong-Seob (Department of Food Science and Technology, College of Natural Resources & Life Science, Pusan National University)
Park, Kyu-Min (Jangmaeul Co.)
Hwang, Dae-Youn (Department of Biomaterial Science, College of Natural Resources & Life Science, Pusan National University)
Publication Information
Journal of Life Science / v.22, no.9, 2012 , pp. 1243-1253 More about this Journal
Abstract
The production and secretion of growth hormone (GH) in the anterior pituitary gland can be induced by several natural products to control cell proliferation, differentiation, and migration. To investigate whether Chungkookjang (CKJ) produced by the fermentation process affects GH-related metabolism, the secretion and the response of GH were observed in pituitary cells and GH target cells. Among six CKJs manufactured by different strains of glycine max, only three CKJs, including Daewon (DW), Daepung (DP), and Taegwang (TG), induced GH secretion from GH3 cells at 5.0 mg/ml concentration. There were no significant changes detected in the viability of any of the cells treated with these CKJs. In addition, the increase in GH secretion from the GH3 cells was dependent on the concentration of the three types of CKJs. The proliferation of cell lines, including MG63 and HepG2 cells, that originated from those derived from the GH target organs was significantly activated by treatment with the GH-containing conditional medium (GCM) harvested from the three CKJ-treated GH3 cells, although their induction rate was different from each other. In these cells, p-STAT5 was maximally translocated into the nucleus of MG63 cells 30 min after DW treatment, while it was translocated in HepG2 cells at 60 min. These results suggest that these three types of CKJ could enhance the secretion of GH, as well as the GCM-derived response, in the two target organs.
Keywords
Chungkookjang (CKJ); growth hormone (GH);
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1 Andreassen, T. T., Jorgensen, P. H., Flyvvbjerg, A., Orskov, H. and Oxlund, H. 1995. Growth hormone stimulates bone formation and strength of cortical bone in aged rats. J. Bone Miner. Res. 10, 1057-1067.
2 Anthony, M. S., Clarkson, T. B., Hughes, J. R., Morgan, T. M. and Burke, G. L. 1996. Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkey. J. Nutr. 126, 43-50.
3 Atti, K. M. 2000. The importance of growth hormone replacement therapy for bone mass in young adults with growth hormone deficiency. J. Pediatr. Endocrinol. Metab. 13, 1011-1021.
4 Campbell, G. S.1997. Growth hormone signal transduction. J. Pediatr. 131 (Suppl), S42.   DOI
5 Carter-Su, C., King, A. P., Argetsinger, L. S., Smit, L. S., Vanderkuur, J. and Campbell, G. S. 1996. Signalling pathway of GH. Endocr. J. 43(Suppl), S65-S70.   DOI
6 Cassidy, A., Bing ham, S. and Setchell, K. 1995. Biological effects of isoflavones in young women: importance of the chemical composition of soybean products. Br. J. Nutr. 74, 587-601.   DOI   ScienceOn
7 Darnell, J. E. Jr, Kerr, I. M. and Stark, G. R. 1994. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 264, 1415-1421.   DOI
8 Gerlier, D. and Thomasset, N. 1986. Use of MTT colorimetric assay to measure cell activation. J. Immunol. Methods 94, 57-63.   DOI   ScienceOn
9 Herrington, J., Smit, L. S., Schwartz, J. and Carter-Su, C. 2000. The role of STAT proteins in growth hormone signaling. Oncogene 19, 2585-2597.   DOI
10 Hong, S. W., Kim, Y. J., Lee, B. K. and Chung, K. S. 2006. The bacterial biological response modifier enriched Cheonggukjang fermentation. Korea J. Food Sci. Technol. 38, 548-553.
11 Ihle, J. N. 1996. STATs: signal transducers and activators of transcription. Cell 84, 331-334.   DOI
12 Ishida, H., Uesugi, T., Hirai, K., Toda, T., Nukaya, H., Yokotsuka, K. and Tsuji, K. 1998. Preventive effects of the plant isoflavones, daidzin, and genistin, on bone loss in ovariectomized rats fed a calcium-deficient diet. Biol. Pharm. Bull. 21, 62-66.   DOI
13 Kajiya, H., Takekoshi, S., Miyai, S., Ikeda, T., Kimura, S. and Osamura, R. Y. 2005. Dietary soybean enhances Pit-1 dependent pituitary hormone production in iodine deficient rats. J. Mol. Histol. 36, 265-274.   DOI
14 Kassem, M., Blum, W. and Risteli, J. 1993. Growth hormone stimulates proliferation and differentiation of normal human osteoblast-like cells in vitro. Calcif. Tissue Int. 52, 222-226.   DOI   ScienceOn
15 Kim, J. S. 1996. Current research trends on bioactive function of soybean. Korea Soybean Digest 13, 17-24.
16 Kim, S. H., Yang, J. L. and Song, Y. S. 1999. Physiological functions of Chungkukjang. Food Indus. Nutr. 4, 40-46.
17 Kim, W. K., Choi, K. H., Kim, Y. T., Park, H. H., Choi, J. Y., Lee, Y. S., Oh, H. I., Kwon, I. B. and Lee, S. Y. 1996. Purification and characterization of a fibrinolytic enzyme produced for Bacillus sp. Strain CK 11-4 screened from Cheonggukjang. Appl. Environ. Microbiol. 62, 2482-2488.
18 Kim, Y. T., Kim, W. K. and Oh, H. I. 1995. General microbiology, physiology and metabolism Screening and identification of the fibrinolytic bacterial strain from Cheonggukjang. Korean J. Microbiol. Biotechnol. 23, 1-5.
19 Koh, J. B. 2006. Effects of Cheonggukjang on lipid metabolism in hyperlipidemic female rats. Korean J. Nutr. 39, 331-337.
20 Kwak, C. S., Kim, M. Y., Kim, S. A. and Lee, M. S. 2006. Cytotoxicity on human cancer cells and antitumorigenesis of Cheonggukjang, a fermented soybean product, im DMBA-treated rats. Korean J. Nutr. 39, 347-356.
21 Kwon, D. Y., Jang, J. S., Lee, J. E., Kim, Y. S., Shin, D. H. and Park, S. 2006. The isoflavonoid aglycone-rich fraction of chungkookjang, fermented unsalted soybeans, enhance insulin signaling and peroxisome proliferator-activated receptor-gamma activity in vitro. Biofactors 26, 245-258.   DOI
22 Lee, E. H. and Chyun, J. H. 2007. Effects of Chongkukjang intake on lipid metabolism and liver function in ethanol consumed rats. Korean J. Nutr. 40, 684-692.   과학기술학회마을
23 Leung, K. C., Howe, C., Gui, L. Y., Trout, G., Veldhuis, J. D. and Ho, K. K. 2002. Physiological and pharmacological regulation of 20-kDa growth hormone. Am. J. Physiol. Endocrinol. Metab. 283, 836-843.
24 Li, L., He, D., Wilborn, T. W., Falany, J. L. and Falany, C. N. 2009. Increased SULT1E1 activity in HepG2 hepatocytes decreases growth hormone stimulation of STAT5b phosphorylation. Steroids 74, 20-29.   DOI
25 Merz-Demlow, B. E., Duncan, A. M., Wangen, K. E., Xu, X., Carr, T. P., Phipps, W. R. and Kurzer, M. S. 2000. Soy isoflavones improve plasma lipids in normocholesterolmic, premenopausal women. Am. J. Clin. Nutr. 71, 1462-1469.
26 Miquet, J. G., Muñoz, M. C., Giani, J. F., González, L., Dominici, F. P., Bartke, A., Turyn, D. and Sotelo, A. I. 2010. Ames dwarf (Prop1(df)/Prop1(df)) mice display increased sensitivity of the major GH-signaling pathways in liver and skeletal muscle. Growth Horm. IGF Res. 20, 118-126.   DOI
27 Perez, F. R., Casabiell, X., Camina, J. P., Zugaza, J. L. and Casanueva, F. F. 1997. cis-unsaturated free fatty acids block growth hormone and prolactin secretion in thyrotropin-releasing hormone-stimulated GH3 cells by perturbing the function of plasma membrane integral proteins. Endocrinology 138, 264-272.   DOI   ScienceOn
28 Potter, S. M., Baum, J. A., Teng, H., Stillman, R. J., Shay, N. F. and Erdman, J. W. Jr. 1998. Soy protein and isoflavones; Their effects on blood lipids and bone density n postemenopausal women. Am. J. Clin. Nutr. 68, 1375-1379.
29 Shim, S. H., Lee, E. J., Kim, J. S., Kang, S. S., Ha, H., Lee, H. Y., Kim, C., Lee, J. H. and Son, K. H. 2008. Rat growth-hormone release stimulators from fenugreek seeds. Chem. Biodivers 5, 1753-1761.   DOI
30 Slootweg, M. C., Swolin, D., Netelenbos, J. C., Isaksson, O. G. and Ohlsson, C. 1997. Estrogen enhances growth hormone receptor expression and growth hormone action in rat osteosarcoma cells and human osteoblast-like cells. J. Endocrinol. 155, 159-164.   DOI
31 Yang, J. L., Lee, S. H. and Song, Y. S. 2003. Improving effect of powders of cooked soybean and Cheonggukjang on blood pressure and lipid metabolism in spontaneously hypertentive rats. J. Korean Soc. Food Sci. Nutr. 32, 899-905.   DOI
32 Yoshizato, H., Fujikawa, T., Shibata, M., Tanaka, M. and Nakashima, K. 1999. Stimulation of growth hormone gene expression in the pituitary and brain by Panax ginseng C. A. MEYER. Endocr. J. 46, 85-88.   DOI