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Molecular subtyping and antimicrobial susceptibility of Streptococcus dysgalactiae subspecies equisimilis isolates from clinically diseased pigs

  • Oh, Sang-Ik;Kim, Jong Wan;Kim, Jongho;So, Byungjae;Kim, Bumseok;Kim, Ha-Young
    • Journal of Veterinary Science
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    • v.21 no.4
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    • pp.57.1-57.11
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    • 2020
  • Background: Streptococcus dysgalactiae subspecies equisimilis (SDSE) acts as an etiological agent for lameness, neurological signs, and high mortality in pigs. Despite its importance in pig industries and zoonotic potential, little is known about the effects of this pathogen. Objectives: This study aimed to determine the molecular characteristics and antimicrobial resistance of SDSE strains isolated from diseased pigs. Methods: A total 11 SDSE isolates were obtained from diseased pigs. Bacterial identification, PCR for virulence genes, emm typing, and antimicrobial resistance genes, multilocus sequence typing, and antimicrobial susceptibility test were performed. Results: Nine isolates were from piglets, and 8 showed lameness, sudden death, or neurological signs. The isolates were PCR-positive for sla (100%), sagA (100%), and scpA (45.5%), and only 1 isolate amplified the emm gene (stL2764). Eight different sequence types were detected, categorized into 2 clonal complexes and 4 singletons. All the isolates in this study were included in a small cluster, which also contained other strains derived from humans and horses. The minimum inhibitory concentrations for the tested beta-lactams were low, while those for macrolides, tetracyclines, and fluoroquinolones were relatively high. PCR analysis of the macrolide and tetracycline resistance genes demonstrated that the isolates carried erm(B) (18.2%, n = 2), mef(A/E) (9.1%, n = 1), tet(M) (18.2%, n = 2), and tet(O) (90.2%, n = 10). Two isolates presented a mutation in parC, which is associated with fluoroquinolone resistance. Conclusion: This study provided insight into swine-derived SDSE, as it is related to veterinary medicine, and elucidated its zoonotic potential, in the context of molecular epidemiology and antimicrobial resistance in public health.

Safety of Perioperative Maintenance of Antiplatelet Agents in Elderly Patients Undergoing Lung Cancer Surgery

  • Hee Ju Hong;Ji Hyeon Park;Samina Park;In Kyu Park;Chang Hyun Kang;Young Tae Kim
    • Journal of Chest Surgery
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    • v.57 no.4
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    • pp.342-350
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    • 2024
  • Background: The maintenance of antiplatelet therapy increases the risk of bleeding during lung cancer surgery. Conversely, the perioperative interruption of antiplatelet therapy may result in serious thrombotic complications. This study aimed to investigate the safety of continuing antiplatelet therapy in the context of lung cancer surgery. Methods: We retrospectively reviewed a cohort of 498 elderly patients who underwent surgery for lung cancer. These patients were categorized into 2 groups: group N, which did not receive antiplatelet therapy, and group A, which did. Group A was subsequently subdivided into group Am, where antiplatelet therapy was maintained, and group Ai, where antiplatelet therapy was interrupted. We compared the incidence of bleeding-related and thrombotic complications across the 3 groups. Results: There were 387 patients in group N and 101 patients in group A (Ai: 70, Am: 31). No significant differences were found in intraoperative blood loss, thoracotomy conversion rates, transfusion requirements, volume of chest tube drainage, or reoperation rates for bleeding control between groups N and A or between groups Am and Ai. The duration of hospital stay was longer for group A compared to group N (7 days vs. 6 days, p=0.005), but there was no significant difference between groups Ai and Am. The incidence of cardiovascular or cerebrovascular complications did not differ significantly between groups Ai and Am. However, group Ai included a severe case of in-hospital ST-elevation myocardial infarction. Conclusion: The maintenance of antiplatelet therapy was found to be safe in terms of perioperative bleeding and thrombotic complications in elderly lung cancer surgery patients.

Effect of Slurry Composting Bio-filtration (SCB) by Subsurface Drip Fertigation on Cucumber (Cucumis sativus L.) Yield and Soil Nitrogen Distribution in Greenhouse

  • Lim, Tae-Jun;Park, Jin-Myeon;Noh, Jae-Seung;Lee, Seong-Eun;Kim, Ki-In
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.4
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    • pp.253-259
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    • 2013
  • The use of subsurface drip fertigation using slurry composting bio-filtration (SCB) as nitrogen (N) fertilizer source can be beneficial to improve fertilizer management decision. The objective of this study was to evaluate effects of SCB liquid fertilizer by subsurface drip fertigation on cucumber (Cucumis sativus L.) yield and soil nitrogen (N) distribution under greenhouse condition. Cucumber in greenhouse was transplanted on April $4^{th}$ and Aug $31^{st}$ in 2012. N sources were SCB and urea. Four N treatments with 3 replications consisted of control (No N fertilizer), SCB 0.5N + Urea 0.5N (50:50 split application), SCB 1.0N, Urea 1.0N. 100% of N recommendation rate from soil testing was denoted as 1.0N. The subsurface drip line and a tensiometer were installed at 30 cm soil depth. An irrigation was automatically started when the tensiometer reading was -15 kPa. The growth of cucumber at 85 days after transplanting was 5% higher in all N treatment than control. Semi-forcing culture produced more fruit yield than retarding culture. Fruit yields were 62.2, 76.3, 76.4, and 75.1 Mg $ha^{-1}$ for control, SCB 1.0N, Urea 1.0N, and SCB 0.5N + Urea 0.5N, respectively. Although fruit yields were similar under SCB 1.0N, Urea 1.0N, and SCB 0.5N + Urea 0.5N, 176 kg K $ha^{-1}$ can be over applied if cucumber is grown twice a year under SCB 1.0N that may result in K accumulation in soil. N uptake was 172, 209, 213, 207 kg $ha^{-1}$ for control, SCB 1.0N, Urea 1.0N, and SCB 0.5N + Urea 0.5N, respectively. N use efficiency was the highest (37%) at SCB 0.5N + Urea 0.5N under semi-forcing culture. Nitrate-N concentration in soil for all N treatments except control in semi-forcing culture was the highest between 15 and 30 cm soil depth at the 85 days after transplanting and between 0 and 15 cm soil depth after cucumber harvest. These results suggested that SCB 0.5N + Urea 0.5N can be used as an alternative N management for cucumber production in greenhouse if K accumulation is concerned.

A Study on the Effective Arc Sensing by the Use of the Weighted-Arc-Current in Flux-Cored Arc Welding for Fillet Joints (가중용접전류를 이용한 FCAW 필릿용접용 아크센싱 알고리즘 연구)

  • 권순창;최재성
    • Journal of Welding and Joining
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    • v.18 no.1
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    • pp.83-90
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    • 2000
  • It was attempted to improve seam-tracking performance by applying a new arc-sensing algorithm for FCAW(flux-cored arc welding) process in fillet joints. For this study the authors have introduced three different weight factors: $\circled1$ arc currents at the weaving end are more weighted, $\circled2$ arc currents are evenly weighted along the weaving, and $\circled3$ arc currents at the weaving center are more weighted. To evaluate the 3 factors the values of signal-to-noise(S/N) ratio has been measured. The values were obtained for various welding conditions with different gaps in horizontal and vertical fillet joints. The test results showed that the S/N ratio of the 1st case was highest which resulted in the best of seam tracking performance. In addition, the comparison between the seam tracking performance in horizontal fillet joints and that in vertical ones has been done, and the result showed that tracking performance in vertical joints was relatively better than that in horizontal joints.

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Effects of Meatal Care in Reduction of Catheter-associated Urinary Tract Infection for Elderly Patients in the ICU (외요도구 간호가 노인중환자의 유치도뇨관 관련 요로감염에 미치는 효과)

  • Shin, Jae-Sun;Sohng, Kyeong-Yae
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.15 no.4
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    • pp.449-456
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    • 2008
  • Purpose: The purpose of this study was to compare the effects of meatal care with 10% betadine or with normal saline on the incidence of urinary tract infection (UTI) for elderly patients with indwelling urinary catheter in the ICU. Method: A quasi experimental design with non-equivalent control group was used. The 37 patients who participated in this study were 65-year-old or older. Patients in the saline group (n=20) received meatal care with normal saline and those in the betadine group (n=17) received meatal care with 10% betadine once a day for 6 days. Urine cultures were done on the 7th day for both groups to detect UTIs. Results: No difference was observed in the incidence of urinary tract infection between the two groups, regardless of patients' gender, ability to communicate or history of operation. Conclusion: The results indicated that use of saline which is cheap and does not irritate the mucous membrane is effective in preventing UTI within the first 7 days, and can be used instead of betadine for meatal care for elderly patients with indwelling urinary catheter in the ICU.

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Effects of Nitrogen Levels and Treatments on Agronomic Characteristics and Yield in Job's Tears(Coix lacryma-Jobi L.) (질소시비수준과 시비방법이 율무의 생육과 수량에 미치는 영향)

  • 권병선;박희진;성낙술
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.5
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    • pp.413-418
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    • 1992
  • The higher fertilizer application level and frequency of additional fertilizer increase the higher the growing characters of plant height, No. of leaves, No. of stem per plant, and No. of ears per main stem, etc., appeared. The yield characters of No. of grains per plant, percentage of threshing, weight of 1 $\ell$, weight of 100 grains and seed yield per 10a appeared high both in used N 14kg, 40% as the basic dressing, 30% as the 1st top dressing, 20% as the 2nd top dressing and 10% as the 3rd top dressing, and in used N 18kg as the total amount of basic dressing. Dispersion analysis showed the difference of significant level according to the interactions, among fertilizer application levels, among fertilizational methods, and between fertilizer application and fertilizational method.

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The Incidence Rate of Ventilator Associated Pneumonia in Relation to the Exchange of Circuit Cycle (인공호흡기 튜브교환주기에 따른 인공호흡기 관련 폐렴발생률)

  • Kim, Nam-Cho;Kim, Yang-Ree
    • Korean Journal of Adult Nursing
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    • v.15 no.3
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    • pp.463-471
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    • 2003
  • Purpose: This study was aimed at providing scientific evidence for minimizing ventilator-associated pneumonia(VAP) by identifying appropriate timing of exchange of circuit for mechanical ventilator that is well suitable for the medical environment of intensive care units of hospitals in Korea. Method: This was a quasi-experimental study with a convenience sample of 19 adult subjects aged over 18 years who were admitted to the NS ICU of C university hospital, and placed on mechanical ventilator. The subjects were placed in two groups, compared on the incidence rate of VAP after they received exchange of circuit either at 1-week interval (N =10) or 2-week interval (N = 9). Result: 1) When considering 1000 days as the standard unit of analysis for incidence, the incidence rate of VAP was 7.19 cases at the 1-week cycle exchange group and 15.23 at the 2-week cycle exchange group, showing no statistically significant difference between the two groups. 2) There were a total of 3 types of bacteria isolated from the patients with VAP, including 2 cases with P. aeruginosa, 1 case with Streptococcus group F and A. baumannii. Conclusion: With thorough hand washing and strict management of tracheal tube of mechanical ventilator as well as use of tracheal intubation techniques, exchange cycle of circuit of mechanical ventilator by nurses may be changed from 1-week to 2-week interval.

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Evaluation of Neuronal Dysfunction in Schizophrenia before and after Neuroleptic Treatment by ??H MRS

  • C, Bo-Young;Paik, In-Ho;Lee, Chang-Uk;Lee, Hyoung-Koo;Suh, Tae-Suk
    • Journal of the Korean Magnetic Resonance Society
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    • v.5 no.1
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    • pp.56-65
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    • 2001
  • Localized in vivo proton magnetic resonance spectroscopy (MRS) was performed to evaluate metabolic alterations in the right and left frontal lobe before and after neuroleptic treatment of schizophrenic patients (n=24) and a group of healthy normal subjects (n=20). Proton metabolic ratios obtained from the 8㎤ yokels in the right and left frontal lobe were compared with the clinical assessment of PANSS for each subject. There was no significant difference in the metabolic ratios between the right and the left frontal lobes in either the schizophrenic group or the control group, indicating no laterality. Compared with those of the normal control group, NAA/Cr and (GABA+Glu)/Cr ratios of the schizophrenic patients showed significantly lower (p=0.023) and higher (p=0.005) value, respectively. The (GABA+Glu)/Cr ratio of the schizophrenic patients was generally decreased after neuroleptic treatment, while the NAA/Cr ratio was not changed. Significant correlation between the (GABA+Glu)/Cr ratio and the clinical symptom scores assessed by PANSS was established. The present study supports the “hypofrontality” hypothesis of schizophrenia on the basis of the altered metabolic ratios before and after neuroleptic treatment.

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Board Level Reliability Evaluation for Package on Package

  • Hwang, Tae-Gyeong;Chung, Ji-Young
    • Proceedings of the International Microelectronics And Packaging Society Conference
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    • 2007.04a
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    • pp.37-47
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    • 2007
  • Factor : Structure Metal pad & SMO size Board level TC test : - Large SMO size better Board level Drop test : - Large SMO size better Factor : Structure Substrate thickness Board level TC test : - Thick substrate better Board level Drop test : - Substrate thickness is not a significant factor for drop test Factor : Material Solder alloy Board level TC test : - Not so big differences over Pb-free solder and NiAu, OSP finish Board level Drop test : - Ni/Au+SAC105, CuOSP+LF35 are better Factor : Material Pad finish Board level TC test : - NiAu/NiAu is best Board livel Drop test : - CuOSP is best Factor : Material Underfill Board level TC test - Several underfills (reworkable) are passed TCG x500 cycles Board level Drop test : - Underfill lots have better performance than non-underfill lots Factor : Process Multiple reflow Board level TC test : - Multiple reflow is not a significant actor for TC test Board level Drop test : N/A Factor : Process Peak temp Board level TC test : - Higher peak temperature is worse than STD Board level Drop test : N/A Factor : Process Stack method Board level TC test : - No big difference between pre-stack and SMT stack Board level Drop test : - Flux dipping is better than paste dipping but failure rate is more faster

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The Effect of for Major River Project and Kumho River on Nakdong River's Water Quality - Focused on Kangjung-Koryung Weir (4대강 사업과 금호강의 수질이 낙동강 본류에 미치는 영향에 대한 연구 - 강정고령보 공사현장을 중심으로)

  • Hwang, Sam-Jin;Bae, Hun-Kyun;Kim, Hak-Yoon
    • Journal of Environmental Science International
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    • v.22 no.6
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    • pp.695-703
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    • 2013
  • In this study, the effects of four major river project and Kumho River, second biggest branch of Nakdong River, were investigated to provide basic data for proper management of Nakdong River's water quality. Daily sampling processes at three different points, Munsanri (the upper side of Kangjung-Koryung weir), Kangchang (the outlet of the Kumho River) and Samunjin (the lower side of Kangjung-Koryung weir and junction of Kumho River and Nakdong River), were conducted from May 1st 2011 to Sep. 4th 2011. Water samples were analyzed for nine factors, DO, BOD, COD, T-N, T-P, pH, turbidity, SS, and coliform. As demonstrated by the results, concentrations of BOD, T-N, T-P and coliform at Nakdong River were affected by water quality of Kumho River while SS and turbidity were affected by constructions for Kangjung-Koryung weir. Further studies, for example, affects of wastewater treatment facilities, should be followed.