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An efficient strategy for blocking the 1st mitotic cleavage of fish zygote using combined thermal treatment, exemplified by mud loach (Misgurnus mizolepis)

  • Nam, Yoon-Kwon;Park, Geyong-Cheol;Kim, Dong-Soo
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.38-38
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    • 2003
  • Blocking the first mitotic cleavage of the zygote is a key tool for chromosome-set manipulations in fish. We developed an improved method for inducing tetraploidy by blocking the mitosis with a combination of heat shock at 40.5$^{\circ}C$ for 1, 2 or 3 min followed by cold shock at $1.5^{\circ}C$ for 30, 45 or 60 min. When applied during the first cleavage metaphase of mud loach (Misgurnus mizolepis) zygotes, the optimal combination was heat for 2 min followed by cold for 45 min. At 1 month, the frequency of 4N survivors and the yield from total eggs fertilized was 55.7% and 14.4%, respectively, compared to heat shock alone with 20.0% efficiency and 3.6% yield. The effectiveness of the procedure was confirmed by diploid mitotic gynogenesis using transgenic markers. The overall yield of homozygous diploids, 34.0%, was better than that for single heat shock, 17.3%. The tetraploids and homozygous diploids had higher early mortality than normal diploid controls. However at 1 month, the viability of the tetraploids was the same as normal diploids. For gynogenetic diploids, the survival was similar to normal diploids after 3 months. The high efficiency of this new protocol extends the opportunity to study polyploidy in basic and applied research.

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The Karyotypic Analysis of Niwaella multifasciata Wakiya et Mori(Cobitidae), An Endemic Species in Korea (한국(韓國) 특산종(特産種) 수수미꾸리, Niwaella multifasciata Wakiya et Mori(Cobitidae)의 핵형분석(核型分析))

  • Nam, Myung-Mo;Yang, Hong-Jun;Chae, Byung-Soo
    • Korean Journal of Ichthyology
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    • v.3 no.1
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    • pp.36-41
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    • 1991
  • The karyotype of the Niwaella multifasciata, endemic species to the Nakdong River in Korea, is reported for the first time. The sample species, Niwaella multifasciata collected from the Milyang River and the Nam River which are tributaries of the Nakdong River, has a diploid complement of 50 chromosomes composed of 10 metacentric, 28 submetacentric, 12 subtelocentric chromosomes, and 88 arm numbers. The arm number of Niwaella multifasciata was larger than any other species of Cobitidae. Sexual dimophism and intraspecific polymophism of the chromosomes were not observed in this study.

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CAD Data Exchange among Different Commercial Packages (이 기종 시스템간의 CAD 데이터 교환)

  • Kim, Tae-Wook;Hwang, Byeong-eok
    • Journal of the Korean Society of Industry Convergence
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    • v.7 no.4
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    • pp.377-382
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    • 2004
  • This research was about implementing STEP for data exchange among different commercial package(CAD system). STEP was used to share CAD data as follows. ST-Developer implement Solid model was used with a physical file of STEP and represented in a CAD system. And then STEP conversion utility was used to make a neutrality format form in a component modeling. Analysis of Solid model was made possible for CAD system to read in and analyzed Solid model. This research are summarized as follows 1. A basis Model development used Visual C++ for a neutrality data of a programming interface general-use CAD system, and produced Box and Cylinder to a physical file, the result was implemented this in a programming interface to general-use CAD system, it is represented in CAD system as identical one modeling object. 2. STEP was used in different CAD system to utilize to its of each CAD system maximum, CATIA V5R9 user was able to analyze MDT 6 Modeling Arm. 3. STEP was used able to interpret and remodel the existing Solid model.

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Design of LNA and Mixer for Ku-band Receiver (Ku 밴드 수신단을 위한 저잡음 증폭기 및 주파수 혼합기 설계)

  • Choi, Hyuk-Jae;Ko, Jae-Hyeong;Kim, Koon-Tae;Lee, Je-Kwang;Kim, Hyeong-Seok
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.61 no.2
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    • pp.257-262
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    • 2012
  • The Ministry of Information-Communication assigned 18~19GHz frequency band for communication of cabins and platform to link between subway/train and it's station. In this paper, we propose wireless transmission devices which are 2 stage hybrid low noise amplifier of 18GHz band and mixer for 18GHz as well to apply for RF receiver. We designed LNA to be noise matched its 1st stage and gain matched for 2nd stage and mixer using $180^{\circ}$ hybrid coupler to suppress the spurious signal. The transistors of 18 GHz LNA and mixer are NE3210S01 of NEC and KMB-N51-1, respectively. As the result of simulation, we get 19.92dB gain and 2.06dB noise figure with LNA and 8.61dB conversion loss with mixer.

Research on the Needs of Learners for Cooking Practice Education (조리실습교육에 대한 학습자의 요구도 조사)

  • 복혜자
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.1
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    • pp.74-82
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    • 2004
  • The study was aimed to estimate the degree of recognition of, satisfaction with and needs for cooking practice education. 300 female students of middle schools and high schools in Seoul, Kyung-gi, Inchon area were targeted fer the research, which has been conducted from January 1st to December 20th in 2003. The crosstab, the t-test, and the ANOVA analysis were processed as methods using SPSS. The study showed that most of students had experienced cooking exercises, and the degree of satisfaction of the high school group was higher than that of the middle school group. Both groups answered they wanted more time for the cooking practice classes and the middle school group had the greater necessity. The necessity far practice lessons of cooking traditional foods reaches n high level in both groups, All the students answered they liked both western and traditional foods, but preferred the traditional food to the western one. They also believed the traditional food was more healthy than the western one. In terms of the needs for cooking practice education, the middle school group wanted to learn how to cook cake, pie, steak potato chip, and hamburger, while the high school group wanted cake, pie, steak sandwich, and potato chip. As for the traditional food, the middle school group wanted to learn how to cook Naegmyun(cold noodles), fried rice, rice hash, dumpling soup, and knife-cut noodles. The high school group, however, picked knife-cut noodles, fried rice, iced noodles, rice hash, and rice-cake soup.

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임신부의 건강이 신생아의 체중과 신장에 미치는 영향

  • 김영매
    • Journal of Korean Academy of Nursing
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    • v.1 no.1
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    • pp.49-58
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    • 1970
  • For the purpose to clarify the effects of the mothers health during pregnancy on the weight and length of the newborn infants, this study was carried out on 500 new born infants delivered at St, Mary's Hospital, Ewha Woman's University Hospital and Red Cross Hospital in Seoul during the eight-month period from March, 1969 to October, 1969. In this study, the relationship between the T weight and length of the new born infants, and in each case, the weight, length, age, duration of labour, duration of pregnancy, weight gain during pregnancy and diets level during pregnancy of the mother were observed. The relationship between the weight and length of the new born infants and the parity n-as also observed. The results obtained in this study were as follows. 1. The weight of new born infants of both sexes was significantly correlated with the parity, the weight of the mother, and the duration of labour of the primipara. The weight of the mother and the duration of pregnancy were significantly correlated with the weight of the male new born infants only, and the weight of the new born infants and the weight gain of the mother during pregnancy were significantly correlated in the female new born infants. 2. In general, the weight of the new born infants of well or excellently nourished mothers were heavier than those of poorly nourished ones. 3. The length of the new born infants was significantly correlated mother the duration of labour in the primipara.

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Overlap Syndrome:Obstructive Sleep Apnea-Hypopnea Syndrome in Patients with Chronic Obstructive Pulmonary Disease (중첩증후군:만성 폐쇄성 폐질환을 가 진 폐쇄성 수면무호흡-저호흡 증후군)

  • Choi, Young-Mi
    • Sleep Medicine and Psychophysiology
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    • v.15 no.2
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    • pp.67-70
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    • 2008
  • Overlap syndrome can be defined as a coexistence of chronic obstructive pulmonary disease (COPD) and sleep apnea-hypopnea syndrome (SAHS). The association of COPD and SAHS has been suspected because of the frequency of both diseases. Prevalence of COPD and SAHS is respectively 10 and 5% of the adult population over 40 years of age. However, a recent study has shown that the prevalence of SAHS is not higher in COPD than in the general population. The coexistence of the two diseases is only due to chance. SAHS does not affect the pathophysiology of COPD and vice versa. Prevalence of overlap syndrome is expected to occur in about 0.5% of the adult population over 40 years of age. Patients with overlap syndrome have a more profound hypoxemia, hypercapnia, and pulmonary hypertension when compared with patients with SAHS alone or usual COPD patients without SAHS. To treat the overlap syndrome, nocturnal noninvasive ventilation (NIV) or nasal continuous positive airway pressure (nCPAP) can be applied with or without nocturnal oxygen supplement.

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Eco-design Color Trends in Fashion (패션에 나타난 에코디자인의 색채경향 연구)

  • Song, Jee-Eun;Choi, Kyung-Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.34 no.3
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    • pp.492-507
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    • 2010
  • The eco-design in modem fashion is mixed with a well-being trend that combines functionality and ethics with technology to suggest a new fashion style for the $21^{st}$ century. This study analyzes the colors of the eco-design in the current fashion trends and identifies the eco-friendly color images that suggest directions for eco-design. The results of this study are as follows. First, the eco-design is an ethical design concept of the ecological value. It can be classified into the functional efficiency in the multi-functionality the sustainability, the recycling capability, the trends for health and craftsmanship, according to various suggestions by eco-design related theorists. Second, the eco-colors shown in the fashion trend follow the order of YR, R, Y, N, and B, with the order of the pale, grayish, light grayish and dull tones. Third, the hue trends of eco-colors each eco-type are focused on R, YR, and Y. With regards to the tones, the functional efficiency, the multi-functional capability, and the sustainability are shown in the neutral tone, while the recycling capability is shown in the grayish tone and the trends pursuing health and craftsmanship are shown in the pale tone.

Cooperative Multi-relay Scheme for Secondary Spectrum Access

  • Duy, Tran-Trung;Kong, Hyung-Yun
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.4 no.3
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    • pp.273-288
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    • 2010
  • In this paper, we propose a cooperative multi-relay scheme for a secondary system to achieve spectrum access along with a primary system. In the primary network, a primary transmitter (PT) transmits the primary signal to a primary receiver (PR). In the secondary network, N secondary transmitter-receiver pairs (ST-SR) selected by a centralized control unit (CCU) are ready to assist the primary network. In particular, in the first time slot, PT broadcasts the primary signal to PR, which is also received by STs and SRs. At STs, the primary signal is regenerated and linearly combined with the secondary signal by assigning fractions of the available power to the primary and secondary signals respectively. The combined signal is then broadcasted by STs in a predetermined order. In order to achieve diversity gain, STs, SRs and PT will combine received replicas of the primary signal, using selection combining technique (SC). We derive the exact outage probability for the primary network as well as the secondary network. The simulation results are presented to verify the theoretical analyses.

A story of literature n acupuncture & moxibustion techniques to treat 10 kinds of lumbago described by Huh Jun in DongUiBoGam(東醫寶鑑) (동의보감(東醫寶鑑) 두통분류(頭痛分類)에 따른 십종두통의(十種頭痛義) 침구치료(鍼灸治療)에 대(對)한 문헌연구(文獻硏究))

  • Ji, Jun-Hwan;Lee, Joon-Moo
    • Korean Journal of Acupuncture
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    • v.21 no.4
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    • pp.167-177
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    • 2004
  • Objective : The purpose of this study is as follows. We classify headache into ten kinds as mentioned in DongUiBoGam(東醫寶鑑), and are going to present each acupuncture & moxibustion treatment. Methods : For the purpose of looking for each treatment, we referred to a. large number of literature for headache treatment from ancient to malearn. Results : The ten kinds of headache have each treatment. above all, In treatment of headache due to middle - headache (正頭痛) and megrim(偏頭痛) have pain in the formable of ache part. Therefore, general point such as GV20, GV23, ST8, TE23, Extra Meridian are used in turn, also wind-cold-headache(風寒頭痛) and many kind of headache such as GV16, LI4, GB20 are mainly used much in turn. Especially BL is in common use. Conclusions : We conclude that ten kinds of lumbago have each different acupuncture & moxibustion points and treatment, so if we follow each treatment we might obtain more higher rate the treatment of headache.

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