• Title/Summary/Keyword: SSU

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Morphology and Molecular Data for Antarctic Cryophilic Microalga, Porosira pseudodenticulata (남극 호냉성 미세조 Porosira pseudodenticulata의 형태와 분자적 자료)

  • Jung, Woong-Sic;Joo, Hyoung-Min;Hong, Sung-Soo;Kang, Jae-Shin;Choi, Han-Gu;Kang, Sung-Ho
    • ALGAE
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    • v.21 no.2
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    • pp.169-174
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    • 2006
  • We have cultured more than 100 Arctic and Antarctic cryophilic microalgal strains in KOPRI culture collections of polar microorganisms (KCCPM). Among them, we tried to identify an Antarctic strain, KOPRI AnM0008 by morphological and molecular analysis. Nuclear SSU rDNA and plastid rbcL sequences were used to identify the strain. It was identified as Porosira pseudodenticulata based on SSU sequence data showing 99% identity with GenBank X85398. This result was supported by morphological features like solitary labiate process, external foramina and internal cribra by optical and scanning electron microscope. Morphological identification and molecular analysis on polar cryophilic microalgae will be accomplished to construct the databases for KCCPM.

Design and Implementation for Augment Reality Application Using Open Source (오픈소스를 활용한 증강현실 어플리케이션 설계 및 구현)

  • Cha, Tae-soo;Kim, Jong-bae;Shin, Yong-tae
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2014.05a
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    • pp.538-541
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    • 2014
  • With the increased market demand for advanced specifications in smart phones, smart phones that have functions with wireless communications with high speed, cameras with high pixel and with high graphic processing ability have appeared. Furthermore, as traditional Augmented Reality technology has been practicable in mobile devices, many application's use AR technology, so AR's portability has been increased. But application with AR technology, which was implemented in smart phones has created capacity issues as applications are taking up a large portion of the memory capacity of phones. This research designed and implemented optimized AR technology by Mixare, AR open source, to solve such problems. As a result, I assured that there has been a decrease in application's memory used on the basis of mobiles.

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Molecular Phylogenetic Relationships Within the Genus Alexandrium(Dinophyceae) Based on the Nuclear-Encoded SSU and LSU rDNA D1-D2 Sequences

  • Kim, Choong-Jae;Sako Yoshihiko;Uchida Aritsune;Kim, Chang-Hoon
    • Journal of the korean society of oceanography
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    • v.39 no.3
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    • pp.172-185
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    • 2004
  • LSU rDNA D1-D2 and SSU rDNA genes of 23 strains in seven Alexandrium (Halim) species, A. tamarense (Lebour) Balech, A. catenella (Whedon et Kofoid), A. fraterculus (Balech) Balech, A. affine (Inoue et Fukuyo) Balech, A. insuetum Balech, A. pseudogonyaulax (Biecheler) Horiguchi ex Yuki et Fukuyo and A. tamiyavanichii Balech, were sequenced and the data were used for molecular phylogenetic analysis. The sequence data revealed 11 and 7 ribotypes in the LSU rDNA D1-D2 region and 4 and 17 ribotypes in the SSU rDNA region of A. catenella and A. tamarense, respectively. Other Alexandrium species had also 1 to 5 ribotypes in the two regions. With the exception of CMC2 and CMC3 of A. catenella, all A. tamarense and A. catenella strains had a common ribotype, a functionally expressed rRNA gene (here termed type A), in both gene regions. In addition to the functionally expressed gene, several pseudogenes were obtained that were found to be good tools to analyze the population designation of regional isolates by grouping them according to shared ribotypes. From the phylogenetic analysis of the sequence data determined in this study and retrieved from GenBank, the genus Alexandrium was divided into 14 groups: 1) A. tamarense, 2) A. excavatum, 3) A. catenella, 4) Tasmanian A. tamarense, 5) A. affine (and/or A. concavum), 6) Thai A. tamarense, 7) A. tamiyavanichii, 8) A. fraterculus, 9) A. margalefii, 10) A. andersonii, 11) A. ostenfeldii, 12) A. minutum (or A. lusitanicum), 13) A. insuetum, and 14) A. pseudogonyaulax. The SSU rDNA gene sequence of A. fundyense was so similar to those of A. tamarense used in this study that the two species were difficult to discriminate each other. A. tamiyavanichii was closest to the A. tamarense strain isolated in Thailand and close to the long chain-forming species of A. affine and A. fraterculus. The phylogenetic tree showed that A. margalefii, A. andersonii, A. ostenfeldii, A. minutum and A. insuetum constituted the basal relative complex, and that A. pseudogonyaulax is an ancestral taxon in the genus Alexandrium.

Sequence Analyses of PCR Amplified Partial SSU of Ribosomal DNA for Identifying Arbuscular Mycorrhizal Fungi in Plant Roots

  • Tae, Moon-Sung;Eom, Ahn-Heum;Lee, Sang-Sun
    • Mycobiology
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    • v.30 no.1
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    • pp.13-17
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    • 2002
  • The genomic DNAs were extracted from roots of Glycine max and Sorghum bicolor, and compared with those from spores of two arbuscular mycorrhizal(AM) fungi, Glomus mosseae and Scutellospora heterogama. The partial small subunit(SSU) of ribosomal RNA genes were synthesized and amplified by polymerase chain reaction with the fungal specific primers, AM1 and NS31. By the recent molecular techniques, the presence of another AM fungal DNA were confirmed in the roots of two plants, and three sequences of rDNA fragments amplified were identified to be close to those of G. caledonium, G. fasiculatum and G. proliferum. The two AM fungi, both, were found to colonize at the cortical layers of plant roots collected in the fields, together.

Development of the Alarm Monitoring System for KNPEC-2 (원자력교육원 2호기 시뮬레이터 경보감시계통 개발)

  • 홍진혁
    • Proceedings of the Korea Society for Simulation Conference
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    • 1999.10a
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    • pp.156-162
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    • 1999
  • 원자력교육원 2회기 기준발전소인 영광 1호기 발전소의 디지털 경보설비 (IAS : Intelligent Annunciator System) 는 MPU, SSU, ACU 및 ADU로 구성되어 있으며, 특히 MPU에서는 SSU 로부터 수신된 Scan Data를 해석하고 경보를 저장하고, 경보의 내용을 프린터 및 CRT를 비롯하여 다양한 형태로 병렬출력이 가능하도록 하고 있다. 본 논문에서는 전력연구원에서 수행중인 '원자력 교육원 시뮬레이터 성능개선' 과제의 일부로 IAS의 구조와 이를 시뮬레이터 주컴퓨터 (Host Computer)상의 다이나믹 모델과 연동시켜 구현하는 방법론을 다루고 있다. 특히, 시뮬레이터에 대한 규제기관의 인허가가 중요한 현안으로 대두될 전망임에 따라 ANSI/ANS-3.5, Regulatory Guide 1.149 등 규제요건에서 제시하는 성능기준 및 검사기준을 만족하도록 설계함으로 향후 규제기관에 대한 인허가 획득에서 대비할 예정이다.

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Determination of Performance Indicator Thresholds Based on Typical PSA Results

  • Kang, Dae-Il;Kim, Kil-Yoo;Hwang, Mee-Jung;Sung, Key-Yong
    • Nuclear Engineering and Technology
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    • v.36 no.6
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    • pp.485-496
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    • 2004
  • Typical probabilistic safety assessment (PSA) results were used to estimate the performance indicator (PI) thresholds of unplanned reactor scram (URS) and safety system unavailability (SSU) for Korean nuclear power plants (NPPs). The changes in core damage frequency (${\Delta}$CDFs) of $10^{-6}/yr$, $10^{-5}/yr$, and $10^{-4}/yr$ were adopted as the risk criteria in setting up the PI thresholds. The PI thresholds for the URS were estimated using information pertaining to the initiating event frequencies, the CDF, and the CDF contribution of each initiating event. The PI thresholds of the SSU were estimated using information on the unavailability, the Fussell-Vesely importance, and the CDF.

Creep Life Prediction of Type 316LN Steel Using Minimum Commitment Method (최소구속법을 이용한 Type 316LN 강의 크리프 수명 예측)

  • Kim W.G.;Yoon S.N.;Ryu W.S.;Yi W.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.10a
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    • pp.295-298
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    • 2005
  • A minimum commitment method (MCM) was applied to predict the creep rupture life of type 316LN SS. For this purpose, a number of the creep rupture data for the type 316LN SS were collected through literature survey and experimental data of KAERl, Using the short-term creep rupture data under 2000 hr, the long-term creep rupture life above $10^5$ hour was predicted by means of the MCM. An optimum value of A, P and G function, used in the MCM equation, was determined respectively, and the creep rupture life with the A values in different temperatures was compared with the experimental data and the predicted curves.

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First Report on Plum Pocket Caused by Taphrina deformans in South Korea

  • Oh, Nam Kwon;Hassan, Oliul;Chang, Taehyun
    • Mycobiology
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    • v.48 no.6
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    • pp.522-527
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    • 2020
  • Plum pocket caused by the dimorphic ascomycetous fungi, Taphrina spp., results in unsightly malformations and crop loss. In 2016, Japanese plums (Prunus salicina Lindl.) with plum pocket symptoms were found in Gimcheon. Three isolates were collected from symptomatic P. salicina fruits and identified as Taphrina deformans based on morphological characteristics and molecular sequence analysis of including internal transcribed space (ITS) and the mitochondrial small ribosomal subunit (SSU) regions of the three isolates. Pathogenicity test on plum fruits confirmed that, the present T. deformans isolates are causal agent of plum pocket. To the best of our knowledge, this is the first report of plum pocket caused by T. deformans in South Korea.