• Ocean Science Journal
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• 제40권3호
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• pp.155-166
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• 2005

New PCR primers (N=18) were designed for the isolation of complete SSU to LSU rDNA sequences from the dinoflagellate Alexandrium tamarense. Standard PCR, employing each primer set selected for amplifications of less than 1.5 kb, successfully amplified the expected rDNA regions of A. tamarense (Korean isolate, HY970328M). Complete SSU, LSU rDNAs and ITS sequences, including 5.8S rDNA, were recorded at 1,800 bp, 520 bp and 3,393 bp, respectively. The LSU rDNA sequence was the first report in Alexandrium genus. No intron was found in the LSU rRNA coding region. Twelve D-domains within the LSU rDNA were put together into 1,879 bp (44.4% G+C), and cores into 1514 bp (42.8% G+C). The core sequence was significantly different (0.0867 of genetic distance, 91% sequence similarity) in comparison with Prorocentrum micans (GenBank access. no. X16108). The D2 region was the longest in length (300 bp) and highly variable among the 12 D-domains. In a phylogenetic analysis using complete LSU rDNA sequences of a variety of phytoplankton, A. tamarense was clearly separated with high resolution against other species. The result suggests that the sequence may resolve the taxonomic ambiguities of Alexandrium genus, particularly of the tamarensis complex.

• ALGAE
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• 제21권2호
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• pp.169-174
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• 2006

We have cultured more than 100 Arctic and Antarctic cryophilic microalgal strains in KOPRI culture collections of polar microorganisms (KCCPM). Among them, we tried to identify an Antarctic strain, KOPRI AnM0008 by morphological and molecular analysis. Nuclear SSU rDNA and plastid rbcL sequences were used to identify the strain. It was identified as Porosira pseudodenticulata based on SSU sequence data showing 99% identity with GenBank X85398. This result was supported by morphological features like solitary labiate process, external foramina and internal cribra by optical and scanning electron microscope. Morphological identification and molecular analysis on polar cryophilic microalgae will be accomplished to construct the databases for KCCPM.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2014년도 춘계학술대회
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• pp.538-541
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• 2014

점차 사람들이 요구하는 스마트폰의 사양이 높아지면서 빠른 속도의 무선통신, 높은 화소의 카메라와 그래픽 처리 능력 등의 기능을 갖춘 고성능의 스마트폰이 등장하였다. 이에 전통적인 AR(Augment Reality) 기술이 모바일 기기에서 가능해지면서 AR이 스마트폰 어플리케이션으로 보급되어 휴대성이 증대 되었다. 하지만 스마트폰에서 구현된 AR은 어플리케이션의 메모리 사용량이 높다는 문제점이 있다. 본 연구는 이러한 메모리 문제점을 해결하기 위하여 증강현실 오픈소스인 Mixare을 활용하여 설계 및 구현하였고 이를 기반으로 모바일 기반 증강현실 어플리케이션의 메모리 사용량의 감소방안을 제시한다.

• Journal of the korean society of oceanography
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• 제39권3호
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• pp.172-185
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• 2004

LSU rDNA D1-D2 and SSU rDNA genes of 23 strains in seven Alexandrium (Halim) species, A. tamarense (Lebour) Balech, A. catenella (Whedon et Kofoid), A. fraterculus (Balech) Balech, A. affine (Inoue et Fukuyo) Balech, A. insuetum Balech, A. pseudogonyaulax (Biecheler) Horiguchi ex Yuki et Fukuyo and A. tamiyavanichii Balech, were sequenced and the data were used for molecular phylogenetic analysis. The sequence data revealed 11 and 7 ribotypes in the LSU rDNA D1-D2 region and 4 and 17 ribotypes in the SSU rDNA region of A. catenella and A. tamarense, respectively. Other Alexandrium species had also 1 to 5 ribotypes in the two regions. With the exception of CMC2 and CMC3 of A. catenella, all A. tamarense and A. catenella strains had a common ribotype, a functionally expressed rRNA gene (here termed type A), in both gene regions. In addition to the functionally expressed gene, several pseudogenes were obtained that were found to be good tools to analyze the population designation of regional isolates by grouping them according to shared ribotypes. From the phylogenetic analysis of the sequence data determined in this study and retrieved from GenBank, the genus Alexandrium was divided into 14 groups: 1) A. tamarense, 2) A. excavatum, 3) A. catenella, 4) Tasmanian A. tamarense, 5) A. affine (and/or A. concavum), 6) Thai A. tamarense, 7) A. tamiyavanichii, 8) A. fraterculus, 9) A. margalefii, 10) A. andersonii, 11) A. ostenfeldii, 12) A. minutum (or A. lusitanicum), 13) A. insuetum, and 14) A. pseudogonyaulax. The SSU rDNA gene sequence of A. fundyense was so similar to those of A. tamarense used in this study that the two species were difficult to discriminate each other. A. tamiyavanichii was closest to the A. tamarense strain isolated in Thailand and close to the long chain-forming species of A. affine and A. fraterculus. The phylogenetic tree showed that A. margalefii, A. andersonii, A. ostenfeldii, A. minutum and A. insuetum constituted the basal relative complex, and that A. pseudogonyaulax is an ancestral taxon in the genus Alexandrium.

• Mycobiology
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• 제30권1호
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• pp.13-17
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• 2002

The genomic DNAs were extracted from roots of Glycine max and Sorghum bicolor, and compared with those from spores of two arbuscular mycorrhizal(AM) fungi, Glomus mosseae and Scutellospora heterogama. The partial small subunit(SSU) of ribosomal RNA genes were synthesized and amplified by polymerase chain reaction with the fungal specific primers, AM1 and NS31. By the recent molecular techniques, the presence of another AM fungal DNA were confirmed in the roots of two plants, and three sequences of rDNA fragments amplified were identified to be close to those of G. caledonium, G. fasiculatum and G. proliferum. The two AM fungi, both, were found to colonize at the cortical layers of plant roots collected in the fields, together.

• 한국시뮬레이션학회:학술대회논문집
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• 한국시뮬레이션학회 1999년도 추계학술대회 논문집
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• pp.156-162
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• 1999

원자력교육원 2회기 기준발전소인 영광 1호기 발전소의 디지털 경보설비 (IAS : Intelligent Annunciator System) 는 MPU, SSU, ACU 및 ADU로 구성되어 있으며, 특히 MPU에서는 SSU 로부터 수신된 Scan Data를 해석하고 경보를 저장하고, 경보의 내용을 프린터 및 CRT를 비롯하여 다양한 형태로 병렬출력이 가능하도록 하고 있다. 본 논문에서는 전력연구원에서 수행중인 '원자력 교육원 시뮬레이터 성능개선' 과제의 일부로 IAS의 구조와 이를 시뮬레이터 주컴퓨터 (Host Computer)상의 다이나믹 모델과 연동시켜 구현하는 방법론을 다루고 있다. 특히, 시뮬레이터에 대한 규제기관의 인허가가 중요한 현안으로 대두될 전망임에 따라 ANSI/ANS-3.5, Regulatory Guide 1.149 등 규제요건에서 제시하는 성능기준 및 검사기준을 만족하도록 설계함으로 향후 규제기관에 대한 인허가 획득에서 대비할 예정이다.

• Nuclear Engineering and Technology
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• 제36권6호
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• pp.485-496
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• 2004

Typical probabilistic safety assessment (PSA) results were used to estimate the performance indicator (PI) thresholds of unplanned reactor scram (URS) and safety system unavailability (SSU) for Korean nuclear power plants (NPPs). The changes in core damage frequency (${\Delta}$CDFs) of $10^{-6}/yr$, $10^{-5}/yr$, and $10^{-4}/yr$ were adopted as the risk criteria in setting up the PI thresholds. The PI thresholds for the URS were estimated using information pertaining to the initiating event frequencies, the CDF, and the CDF contribution of each initiating event. The PI thresholds of the SSU were estimated using information on the unavailability, the Fussell-Vesely importance, and the CDF.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 추계학술대회 논문집
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• pp.295-298
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• 2005

A minimum commitment method (MCM) was applied to predict the creep rupture life of type 316LN SS. For this purpose, a number of the creep rupture data for the type 316LN SS were collected through literature survey and experimental data of KAERl, Using the short-term creep rupture data under 2000 hr, the long-term creep rupture life above $10^5$ hour was predicted by means of the MCM. An optimum value of A, P and G function, used in the MCM equation, was determined respectively, and the creep rupture life with the A values in different temperatures was compared with the experimental data and the predicted curves.

• Mycobiology
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• 제48권6호
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• pp.522-527
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• 2020

Plum pocket caused by the dimorphic ascomycetous fungi, Taphrina spp., results in unsightly malformations and crop loss. In 2016, Japanese plums (Prunus salicina Lindl.) with plum pocket symptoms were found in Gimcheon. Three isolates were collected from symptomatic P. salicina fruits and identified as Taphrina deformans based on morphological characteristics and molecular sequence analysis of including internal transcribed space (ITS) and the mitochondrial small ribosomal subunit (SSU) regions of the three isolates. Pathogenicity test on plum fruits confirmed that, the present T. deformans isolates are causal agent of plum pocket. To the best of our knowledge, this is the first report of plum pocket caused by T. deformans in South Korea.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.114.1-114
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• 1997

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