Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Sequence Analyses of PCR Amplified Partial SSU of Ribosomal DNA for Identifying Arbuscular Mycorrhizal Fungi in Plant Roots

  • Tae, Moon-Sung (Department of Biology, Korea National University of Education) ;
  • Eom, Ahn-Heum (Institute of Natural Science, Korea National University of Education) ;
  • Lee, Sang-Sun (Department of Biology, Korea National University of Education)
  • Published : 2002.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The genomic DNAs were extracted from roots of Glycine max and Sorghum bicolor, and compared with those from spores of two arbuscular mycorrhizal(AM) fungi, Glomus mosseae and Scutellospora heterogama. The partial small subunit(SSU) of ribosomal RNA genes were synthesized and amplified by polymerase chain reaction with the fungal specific primers, AM1 and NS31. By the recent molecular techniques, the presence of another AM fungal DNA were confirmed in the roots of two plants, and three sequences of rDNA fragments amplified were identified to be close to those of G. caledonium, G. fasiculatum and G. proliferum. The two AM fungi, both, were found to colonize at the cortical layers of plant roots collected in the fields, together.

Keywords

References

  1. Ausubel, F. M., Brent, R, Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. 1999. Short protocols in molecular biology. John Wiley and Sons, Inc, New York
  2. Bever, J. D., Morton, J. B., Antonovics, J. and Schultz, P. A 1996. Host-dependent sporulation and species diversity of arbuscular mycorrhizal fungi in a mown grassland. Journal of Ecology 84: 71-82 https://doi.org/10.2307/2261701
  3. Clapp, J. P., Young, J. P. W, Merryweather, J. Wand Fitter, A. H. 1995. Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community. New Phytologist 130: 259-265 https://doi.org/10.1111/j.1469-8137.1995.tb03047.x
  4. Daniels, B. A. and Skipper, H. A 1982. Methods for the recovery and quantitative estimation of propagules from soil. Pp 29-35. In: Methods and principles of mycorrhizal research. N. C. Schenck (Ed.). American Phytopathological Society. St. Paul, Minn
  5. Eom, A H., Hartnett, D. C. and Wilson, G. W T 2000. Host plant species effects on arbuscular mycorrhizal fungal communities in tallgrass prairie. Oecologia 122: 435-444 https://doi.org/10.1007/s004420050050
  6. Gianianazzi, S. and Schuepp, H. 1994. Impact of Arbuscular mycorrhizas on Sustainable Agriculture and Natural Ecosystems. Birkhauser Verlag, Basel
  7. Hall, I. R. 1984. Taxonomy of VA mycorrhizal fungi. Pp 57-94. In: VA-Mycorrhizae. C. L. Powell & D. J. Bagyoargi (Eds.). Baca Raton. CRC
  8. Hall, I. R. and Fish, B. J. 1979. A key to the Endogonaceae. Trans. Br. Mycol. Soc. 73: 261-270 https://doi.org/10.1016/S0007-1536(79)80110-2
  9. Helgason, T, Fitter, A H. and Young, J. P. W. 1999. Molecular diversity of arbuscular mycorrhizal fungi colonizing Hyacinthoides non-scripta (bluebell) in a seminatural woodland. Molecular Ecology 8: 659-666 https://doi.org/10.1046/j.1365-294x.1999.00604.x
  10. Johnson, N. C., Pfleger, F. L., Crookston, R. K. and Simmons, S. R 1991. Vesicular-arbuscular mycorrhizas respond to com and soybean cropping history. New Phytologist 117: 657-664 https://doi.org/10.1111/j.1469-8137.1991.tb00970.x
  11. Koske, R. and Gemma, J. N. 1989. A modified procedure for staining roots to detect VA mycorrhizas. Mycological Research 92: 486-505 https://doi.org/10.1016/S0953-7562(89)80195-9
  12. Lee, J. K. 2002. Studies on the mycorrhizae found in the roots of Korean ferns and its relatives. Korea National University of Education, Chungbuk
  13. Redecker, D., Morton, J. B. and Bruns, T D. 2000. Molecular phylogeny of the arbuscular mycorrhizal fungi Glomus sinuosum and Sclerocystis coremioides. Mycologia 92: 282-285 https://doi.org/10.2307/3761561
  14. Redecker, D., Thierfelder, H., Walker, C. and Werner, D. 1997. Restriction analysis of PCR-amplified internal transcribed spacers of ribosomal DNA as a tool for species identification in different genera of the order Glomales. Applied and Environmental Microbiology 63: 1756-1761
  15. Schenck, N. C. and Perez, Y. 1990 Manual for the Identification of VA Mycorrhizal Fungi Synergistic Publications. Gainesville, Florida
  16. Simon, L., Lalonde, M. and Bruns, T. D. 1992. Specific amplification of 18S fungal ribosomal genes from vesicular- arbuscular endomycorrhizal fungi colonizing roots. Appl. Environ. Microbiol. 58: 291-295
  17. Smith, S. E. and Read, D. J. 1996. Mycorrhizal symbiosis. Academic Press. London
  18. Trappe, J. M. 1982. Synoptic keys to the genera and species of Zygomycetous mycorrhizal fungi. Phytopathology 72: 1102-1108
  19. Van der Heijden, M. G. A, Klironomos, J. N., Ursie, M., Moutoglis, P., Streitwolf, E. R., Boller, T, Wiemken, A. and Sanders, I. R. 1998. Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396: 69-72 https://doi.org/10.1038/23932

Cited by

  1. Roots vol.31, pp.1, 2003, https://doi.org/10.4489/MYCO.2003.31.1.001
  2. vol.31, pp.1, 2003, https://doi.org/10.4489/MYCO.2003.31.1.019
  3. ITS Primers with Enhanced Specificity to Detect the Ectomycorrhizal Fungi in the Roots of Wood Plants vol.31, pp.1, 2003, https://doi.org/10.4489/MYCO.2003.31.1.023
  4. Restriction Analyses of PCR Amplified Partial SSU Ribosomal DNA to Distinguish Arbuscular Mycorrhizal Fungi from Other Fungi Colonizing Plant Roots vol.31, pp.2, 2003, https://doi.org/10.4489/MYCO.2003.31.2.068