• Computational Structural Engineering
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• v.36 no.2
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• pp.14-18
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• 2023

• Reproductive and Developmental Biology
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• v.38 no.4
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• pp.137-142
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• 2014

A tremendous increase in the human population has put poultry industry under an increased pressure to meet steep increase in the demand. Poultry is contributing 25% of the total world's meat production and lesser cost of investment per bird makes it more suitable for the further breeding programmes. Major poultry diseases frequently lead to cardiac damage and cause huge economic losses to poultry industry due to mortality. The in vitro embryonic stem cell (ESC) technology has a futuristic approach for homogeneous populace of differentiated cells, for their further transplantations. During in vitro conditions the differentiated cell populace can be used in grafting and transplantation processes to regenerate damaged tissues. Therefore, the current study targeted the use of spermatogonial stem cells (SSCs) in the poultry production system through cardiac regeneration. The current study will also open new boulevard for the similar kind of research in other livestock species for the management of heart diseases.

• The Transactions of the Korea Information Processing Society
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• v.7 no.8S
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• pp.2766-2773
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• 2000

본 논문에서는 HLRP(High-reliable Light-weight Real-time Protocol)라는 실시간 통신 프로토콜에 대하여 기술한다. HLRP는 SSCS(Service Specific Convergence Sublayer)계층의 프로토콜로서 ATM 교환 시스템 내에서 동등계층간(peer-to-peer)의흐름제어, 전송 오류 복구 및 IPC를 위한 통신 링크 관리 등의 기능을 수행한다. 일반적으로 SSCS 프로토콜은 통신 링크를 설정하고 해제하는 과정을 필요로 하나 HLRP에서는 그 과정을 필요로 하지 않는 다. HLRP는 ATM 교환 시스템과 같은 전용시스템에서 처리기나 제어기와 같은 통신 객체들에 관한 정보를 시스템 구성 시에 알 수 있다는 점을 이용하여 항구가상연결 개념을 적용한 경량의 실시간 프로토콜이다. 또한 오류 복구 절차를 간소화하고 프로토콜의 상태 수를 줄임으로써 성능을 높였다. 실험을 통하여 HLRP가 경량임에도 불구하고 ATM 교환 시스템에서 요구하는 고 신뢰도를 만족함을 보였다.

• Nuclear Engineering and Technology
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• v.55 no.7
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• pp.2489-2497
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• 2023

Co and Eu impurities in the SSCs are nuclides that dominantly influence the neutron-induced radioactive inventory in metal and concrete radwastes (radioactive wastes) during NPP decommission. The impurity concentrations provided by NUREG/CR-3474 were used for the practical range of Co and Eu impurity concentrations to be applied to the code calculations. Metal structures near the core were evaluated to be ILW (intermediate-level waste) for the whole range of Co impurity concentration, so the boundary line between ILW and LLW (low-level waste) has no change for the whole concentration range provided by NUREG/CR-3474. Also, the boundary line between VLLW (very low-level waste) and CW (clearance waste) in the concrete shield could alter a little depending on the Eu impurity concentration within the range provided by NUREG/CR-3474. From this work, it is found that the concentration of material impurities of SSCs gives no critical impact on determining radwaste levels.

• Bulletin of the Korean Space Science Society
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• 2003.05b
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• pp.19-19
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• 2003

No Abstract, See Full Text

• 대한생식의학회:학술대회논문집
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• 2007.11a
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• pp.43-49
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• 2007

• Korean Journal of Remote Sensing
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• v.27 no.6
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• pp.677-692
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• 2011

Suspended sediment is an important oceanic variable for monitoring changes in coastal environment related to physical and biogeochemical processes. In order to estimate suspended sediment concentration (SSC) from satellite data, we derived SSC coefficients by fitting satellite remote sensing reflectances to in-situ suspended sediment measurements. To collect in-situ suspended sediment, we conducted ship cruises at 16 different locations three times for the periods of Sep.-November 2009 and Jul. 2010 at the passing time of Landsat $ETM_+$. Satellite data and in-situ data measured by spectroradiometers were converted to remote sensing reflectances ($R_{rs}$). Statistical approaches proved that the exponential formula using a single band of $R_{rs}$(565) was the most appropriate equation for the estimation of SSC in this study. Satellite suspended sediment using the newly-derived coefficients showed a good agreement with insitu suspended sediment with an Root Mean Square (RMS) error of 1-3 g/$m^3$. Satellite-observed SSCs tended to be overestimated at shallow depths due to bottom reflection presumably. This implies that the satellite-based SSCs should be carefully understood at the shallow coastal regions. Nevertheless, the satellite-derived SSCs based on the derived SSC coefficients, for the most cases, reasonably coincided with the pattern of in-situ suspended sediment measurements in the study region.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.11C
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• pp.1152-1157
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• 2002

In this paper, a detailed procedure of development for an AAL2 processor widely used in voice gateway application is introduced. The processor supports CPS and SSCS with voice service and framed mode data service. It provides 4 ATM virtual connections, which include 1020 AAL2 channels. The processor has one UTOPIA Level 1 interface for an ATM cell interface and 4 TDM ports for a voice channel interface. The TDM ports carry PCM/ADPCM voice streams. Most AAL2 processors are implemented as software, or hardware and software, so its latency is large. But this processor has very low latency as to CPS and SSCS because all of them are implemented in hardware. Also, it allows not only loopback and switching of CPS packets, but loopback and switching of TDM channels. The key feature is that the internal structure of the CPS and SSCS in this processor seems like as each software function, so they are called whenever they are required. In addition, they are reusable for another design and are scalable for more channels.

• Molecules and Cells
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• v.41 no.7
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• pp.631-638
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• 2018

Spermatogonial stem cells (SSCs) derived from mouse testis are unipotent in regard of spermatogenesis. Our previous study demonstrated that SSCs can be fully reprogrammed into pluripotent stem cells, so called germline-derived pluripotent stem cells (gPS cells), on feeder cells (mouse embryonic fibroblasts), which supports SSC proliferation and induction of pluripotency. Because of an uncontrollable microenvironment caused by interactions with feeder cells, feeder-based SSC reprogramming is not suitable for elucidation of the self-reprogramming mechanism by which SSCs are converted into pluripotent stem cells. Recently, we have established a Matrigel-based SSC expansion culture system that allows longterm SSC proliferation without mouse embryonic fibroblast support. In this study, we developed a new feeder-free SSC self-reprogramming protocol based on the Matrigel-based culture system. The gPS cells generated using a feeder-free reprogramming system showed pluripotency at the molecular and cellular levels. The differentiation potential of gPS cells was confirmed in vitro and in vivo. Our study shows for the first time that the induction of SSC pluripotency can be achieved without feeder cells. The newly developed feeder-free self-reprogramming system could be a useful tool to reveal the mechanism by which unipotent cells are self-reprogrammed into pluripotent stem cells.

• Toxicological Research
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• v.33 no.2
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• pp.107-118
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• 2017

Although alternative test methods based on the 3Rs (Replacement, Reduction, Refinement) are being developed to replace animal testing in reproductive and developmental toxicology, they are still in an early stage. Consequently, we aimed to develop alternative test methods in male animals using mouse spermatogonial stem cells (mSSCs). Here, we modified the OECD TG 489 and optimized the in vitro comet assay in our previous study. This study aimed to verify the validity of in vitro tests involving mSSCs by comparing their results with those of in vivo tests using C57BL/6 mice by gavage. We selected hydroxyurea (HU), which is known to chemically induce male reproductive toxicity. The 50% inhibitory concentration ($IC_{50}$) value of HU was 0.9 mM, as determined by the MTT assay. In the in vitro comet assay, % tail DNA and Olive tail moment (OTM) after HU administration increased significantly, compared to the control. Annexin V, PI staining and TUNEL assays showed that HU caused apoptosis in mSSCs. In order to compare in vitro tests with in vivo tests, the same substances were administered to male C57BL/6 mice. Reproductive toxicity was observed at 25, 50, 100, and 200 mg/kg/day as measured by clinical measures of reduction in sperm motility and testicular weight. The comet assay, DCFH-DA assay, H&E staining, and TUNEL assay were also performed. The results of the test with C57BL/6 mice were similar to those with mSSCs for HU treatment. Finally, linear regression analysis showed a strong positive correlation between results of in vitro tests and those of in vivo. In conclusion, the present study is the first to demonstrate the effect of HU-induced DNA damage, ROS formation, and apoptosis in mSSCs. Further, the results of the current study suggest that mSSCs could be a useful model to predict male reproductive toxicity.