• Title/Summary/Keyword: SSC7

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Relationship between porcine miR-20a and its putative target low-density lipoprotein receptor based on dual luciferase reporter gene assays

  • Ding, Yueyun;Zhu, Shujiao;Wu, Chaodong;Qian, Li;Li, DengTao;Wang, Li;Wan, Yuanlang;Zhang, Wei;Yang, Min;Ding, Jian;Wu, Xudong;Zhang, Xiaodong;Gao, Yafei;Yin, Zongjun
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.7
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    • pp.922-929
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    • 2019
  • Objective: Mutations in low-density lipoprotein receptor (LDLR), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC-miR-20a. Methods: Bioinformatic analysis predicted that porcine LDLR is a target of SSC-miR-20a. Wild-type and mutant LDLR 3'-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3'-UTR and pGL3 Control LDLR mutant-3'-UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine premiR-20a-coding sequence between the HindIII and BamHI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3'-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results: Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR3'-UTR-driven (p<0.01), but not mutant LDLR-3'-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = -0.656, p<0.05). Conclusion: LDLR is a potential target of miR-20a, which might directly bind the LDLR 3'-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.

Photoelectric Conversion Efficiency of DSSC According to Plasma Surface Treatment of Conductive Substrate (전도성 기판의 플라즈마 처리에 따른 염료감응형 태양전지 광전변환 효율 특성 변화)

  • Ki, Hyun-Chul;Kim, Seon-Hoon;Kim, Doo-Gun;Kim, Tae-Un;Hong, Kyung-Jin;So, Soon-Yeol
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.25 no.11
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    • pp.902-905
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    • 2012
  • This study is explore the photoelectric conversion change of dye-sensitized solar cells with surface treatment of the conductive substrate. gases of FTO surface treatment were $N_2$, and $O_2$. Treatment conditions of surface were gas flux from 25 sccm to 50 sccm and RF power were from 25 W to 50 W. Treatment time and pressure were fixed 5 min and 100 mtoor. The best sheet resistance and surface roughness were obtained by $O_2$ 50 sccm and 50 W and that result were 7.643 ${\Omega}/cm^2$ and 17.113 nm, respectively. The best efficiency result was obtained by $O_2$ 50 sccm and 50 W and that result of Voc, Jsc, FF and efficiency were 7.03 V, 14.88 $mA/cm^2$, 63.75% and 6.67%, respectively.

On the Types and Functions of English Subordination including Smallest Small Clauses (영어 종속접속의 유형과 기능: 극소절을 포함하여)

  • Hong, Sungshim
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.1
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    • pp.134-139
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    • 2021
  • This paper discusses the types and functions of English subordinate clauses, whether English subordinate clauses (SC) are headed by a Complementizer (CP) or headed by a lexical (but not functional) Preposition (PP). Furthermore, unlike the standard classification, the current paper provides a finer-grained analysis and classification of English SCs. The traditional or prescriptive view on the functions of English SCs includes Noun SC such as complement clauses, Adjectival SC including relative clauses, and Adverbial SCs that cover a garden variety of subordinators. Added to the existing classification of subordination in English is what I notate as Verbless subordinate clause (V-less SC). Of these 4 different types of subordinate clauses with different functions, properties, and distributions, Subjectless Verbless subordinate clause is further divided into Smallest small clause (SSC) which accounts for English subordination mechanism more uniformly and consistently with respect to their clausal architecture, especially when the subordinate clause is neither PP nor CP.

Identification of Quantitative Trait Loci(QTL) for Meat Color Trait on Chromosome 7 in Pig (돼지 7번 염색체에서 육색 연관 QTL 확인)

  • Choi, B.H.;Lee, H.Y.;Kim, T.H.;Hong, K.C.;Cheong, I.C.
    • Journal of Animal Science and Technology
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    • v.46 no.4
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    • pp.525-536
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    • 2004
  • The objective of this study was to identify the quantitative traits loci(QTL) for meat quality traits in pigs. Three-generation resource population was constructed from a cross between Korean native boars and Landrace sows. The resource population including founders, $F_1$ and $F_2$ was genotyped for 23 microsatellite markers on chromosome 7. The sex average total length of linkage map on chromosome 7 was estimated 154.6 cM. Meat quality traits including meat pH, meat color, drip loss, shear force, heating loss, crude fat, crude protein, crude ash and water content in muscle were collected from $F_2$ animals. For the QTL mapping, we used $F_2$ QTL Analysis Servlet of QTL express for web-based QTL mapping tools(http://qtl.cap.ed.ac.uk/). The QTLs for CIE-a and CIE-b on SSC7 were significantly detected at 1% and 5% chromosome-wide level, respectively.

Molecular Characterization, Chromosomal Localizations, Expression Profile, and Association Analysis of the Porcine PECI Gene with Carcass Traits

  • Gao, H.;Fan, B.;Zhu, M.J.;Liu, Bang
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.1
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    • pp.7-12
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    • 2010
  • The full-length cDNA of the porcine peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase (PECI) gene encodes a monofunctional peroxisomal ${\Delta}^3$,${\Delta}^2$-enoyl-CoA isomerase. Cloning and sequencing of the porcine PECI cDNA revealed the presence of an 1185-base pair open reading frame predicted to encode a 394-amino acid protein by the 5'rapid amplification of cDNA ends (5'RACE) and EST sequences. The porcine PECI gene was expressed in seven tissues (heart, liver, spleen, lung, kidney, skeletal muscle, fat) which was revealed by reverse transcriptase-polymerase chain reaction (RT-PCR). The porcine PECI was mapped to SSC71/2 p11-13 using the somatic cell hybrid panel (SCHP) and the radiation hybrid panel (RH) (LOD score 12.84). The data showed that PECI was closely linked to marker S0383. A C/T single nucleotide polymorphism in PECI exon 10 (3'UTR) was detected as a PvuII PCR-RFLP. Association analysis in our experimental pig population showed that different genotypes of PECI gene were significantly associated with the Average Backfat thickness (ABF) (p<0.05) and Buttock backfat thickness (p<0.01).

Turbidity One-System for River and Reservoir (하천-저수지 탁수 일원화 모델 )

  • Seokil Jeong;Eun Ji Kim;Cheol Seo Ki;;EJae Kwon;Hwa Young Kim
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.510-510
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    • 2023
  • 집중호우의 증가는 저수지 내 탁수의 증가로 이어지며, 저수지에서의 탁수 배제는 하천의 탁수 문제로 연결될 수 있다. 탁수의 발생은 정수처리 비용 증가와 하천 생태계 문제 등을 발생시키므로, 이에 대한 해결책이 지속적으로 요구되고 있다. 특히 낙동강 유역의 임하호는 태풍 및 집중호우 발생 시 고탁수의 유입 빈도가 높다. 탁수가 임하호에 유입되면 일반적으로 저수지 탁수 예측 수치해석을 수행하여 탁수의 저수지 내 탁수 거동과 방류량에 따른 탁수 저감 정도를 파악하고, 탁수 배제를 위한 저수지 운영계획을 수립한다. 그러나 방류된 고탁수의 하천 거동에 대한 수치해석은 국내에서는 수행하고 있지 않은 실정이다. 임하댐 하류에서 내성천합류부까지는 약 0.325 m3/s의 물을 취수하고 있으므로(2022년 기준), 하천의 탁수도 저수지 탁수와 동시에 고려되어야 할 필요가 있다. 저수지와 하천의 유사 거동 분석에 대해 격자 구성 등의 이유로 일반적으로 각각 다른 tool을 사용하고 있으나, 모델구축 및 입□출력 data의 변환 등 구축, 구동, 분석 과정 모두 효율적이지 못하다. 이에 본 연구에서는 하천과 저수지에서의 탁수 거동을 동시에 예측할 수 있는 일원화된 수치해석 모델을 구축(임하호~낙본C)하였다. 준3차원 모델인 EFDC를 이용하였으며, 격자는 저수지는 직사각격자, 하천은 Curvlinear를 적용하였다. 연직방향으로는 저수지와 하천의 수심 차이가 크므로 EFDC 내에 탑재되어 있는 𝜎-z(uniform)레이어 옵션을 선택하여, 저수지 연직 레이어는 40개, 하천은 2개로 구축되었다. 하천에서의 탁수 거동 예측은 지류의 유량, 탁도 등의 경계조건 data를 얻기가 쉽지 않기 때문에, 유량은 임하호 유입유량에 유역면적비를 적용하였으며, 탁도는 TSS(Total Suspended Solid)-SSC(Suspended Sediment Concentration)-유량의 관계를 각각 회귀분석 등을 통해 도출하여 연구 범위 내 7개 지류하천에 적용하였다. 모의 시기는 2개의 태풍으로 연속적인 탁수가 발생한 2020년 08~10월로 결정하였다. 모의 결과 저수지와 하천의 PBIAS는 각각 13.6과 8.4로(Very Good 등급) 하천과 저수지를 동시에 모의하는 탁수 예측의 적용 가능성은 충분한 것으로 확인되었다. 그러나 이벤트별 지류의 유량과 탁도는 일정한 관계로 계산될 수 없으므로, 향후 유역 모델과 연계하여 지류 유입 유량 및 탁도의 정확도를 개선할 것이다.

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Effects of 1-methylcyclopropene (1-MCP) treatment on the maintenance of fruit quality of RubyS apples during cold storage ('루비에스' 사과의 저온저장 중 과실품질 유지를 위한 1-methylcyclopropene 처리 효과)

  • Jingi Yoo;Hwajong Yoo;Nay Myo Win;Hee-Young Jung;Young-Je Cho;In-Kyu Kang
    • Food Science and Preservation
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    • v.30 no.1
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    • pp.78-87
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    • 2023
  • This study was conducted to evaluate the effect of different 1-methylcyclopropene (1-MCP) concentrations on fruit quality of small-sized RubyS apples during cold storage. After harvesting, the fruits were treated with 1-MCP at 0.5 or 1 µL/L concentrations and, subsequently, stored at 0℃ for 6 months. After 6 months, the flesh firmness of untreated fruits, which was 85.4 N at harvest, had gradually decreased to 46.5 N; however, that of 1-MCP-treated fruits was maintained at 59.1 and 59.5 N. Titratable acidity (TA) of untreated fruits had also decreased from 0.42 to 0.24%, whereas that of 1-MCP-treated fruits was maintained at 0.26 and 0.27%. Soluble solids content (SSC) did not differ in all fruits. However, the 1-MCP-treated fruits had lower levels of SSC/TA ratio than untreated fruits. Moreover, after 6 months, the ethylene production had increased to 47.0 µL/kg/h in the untreated fruits, whereas 1-MCP blocked the ethylene production at 1.4 and 1.7 µL/kg/h. The weight loss and peel color variables remained unaffected by 1-MCP treatments. Thus, these results indicated that, for RubyS apples, the storability was only 2 months at 0℃ without treatment, which can be extended to 6 months with 1-MCP treatments. The application of 1-MCP at 0.5 µL/L concentration is effectively and economically sufficient to maintain the quality of RubyS apples.

Sequencing, Genomic Structure, Chromosomal Mapping and Association Study of the Porcine ADAMTS1 Gene with Litter Size

  • Yue, K.;Peng, J.;Zheng, R.;Li, J.L.;Chen, J.F.;Li, F.E.;Dai, L.H.;Ding, SH.H.;Guo, W.H.;Xu, N.Y.;Xiong, Y.ZH.;Jiang, S.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.7
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    • pp.917-922
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    • 2008
  • A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTS1 gene was obtained. Analysis of the ADAMTS1 gene using the porcine radiation hybrid panel indicated that pig ADAMTS1 is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTS1 as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).

Quality of 'Fuji' Apple Packed with Functional MA Film to the Films Thickness (두께별 기능성 MA 필름으로 포장하여 저장한 사과의 품질)

  • Hong, Sang Tai;Park, HyungWoo;Park, Yang-Kyun
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.25 no.2
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    • pp.51-56
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    • 2019
  • The quality of 'Fuji' apple was analyzed the film thickness conditions and packed with functional MA film during 24 weeks storage at $0^{\circ}C$ to weight loss, hardness, acidity and SSC, decay ratio, and vitamin C. The weight loss of apple packed functional MA film was higher than that of contro.Value of the weight loss of apple packed in the control and functional MA filmswere 8.2% and 0.4-0.5%, respectively. The change of hardness and titratable acidity was also lower than that of control. The decay ratio of apple packed with functional MA films were 2.4~4.7%, but that of control was 6.8%. The decay ratio of apple was the lowest at 0.025mm functional MA film. Changes of vitamin C also was lower than that of control. Based on the results, we suggested that the functional MA film with 0.025mm thickness is desirable for keeping the quality of the 'Fuji' apple produced at Geochang, Korea.

Development of Heat-Health Warning System Based on Regional Properties between Climate and Human Health (대도시 폭염의 기후-보건학적 특성에 기반한 고온건강경보시스템 개발)

  • Lee, Dae-Geun;Choi, Young-Jean;Kim, Kyu Rang;Byon, Jae-Young;Kalkstein, Laurence S.;Sheridan, Scott C.
    • Journal of Climate Change Research
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    • v.1 no.2
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    • pp.109-120
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    • 2010
  • Heat wave is a disaster, which increases morbidity and mortality in temperate regions. Climate model results indicate that both intensity and frequency of heat wave in the future will be increased. This study shows the result about relationship between excess mortality and offensive airmass in 7 metropolitan cities, and an operational Heat-Health Warning System (HHWS) in Korea. Using meteorological observations, the Spatial Synoptic Classification (SSC) has been used to classify each summer day from 1982 to 2007 into specific airmass categories for each city. Through the comparative study analysis of the daily airmass type and the corresponding daily mortality rate, Dry Tropical (DT), and Moist Tropical plus (MT+) were identified as the most offensive airmasses with the highest rates of mortality. Therefore, using the multiple linear regression, forecast algorithm was produced to predict the number of the excess deaths that will occur with each occurrence of the DT and MT+ days. Moreover, each excess death forecast algorithm was implemented for the system warning criteria based on the regional acclimatization differences. HHWS will give warnings to the city's residents under offensive weather situations which can lead to deterioration in public health, under the climate change.