• Molecules and Cells
• /
• v.23 no.3
• /
• pp.370-378
• /
• 2007

A superoxide dismutase was purified 62-fold in seven steps to homogeneity from Methylobacillus sp. strain SK1, an obligate methanol-oxidizing bacterium, with a yield of 9.6%. The final specific activity was 4,831 units per milligram protein as determined by an assay based on a 50% decrease in the rate of cytochrome c reduction. The molecular weight of the native enzyme was estimated to be 44,000. Sodium dodecyl sulfate gel electrophoresis revealed two identical subunits of molecular weight 23,100. The isoelectric point of the purified enzyme was found to be 4.4. Maximum activity of the enzyme was measured at pH 8. The enzyme was stable at pH range from 6 to 8 and at high temperature. The enzyme showed an absorption peak at 280 nm with a shoulder at 292 nm. Hydrogen peroxide and sodium azide, but not sodium cyanide, was found to inhibit the purified enzyme. The enzyme activity in cell-free extracts prepared from cells grown in manganese-rich medium, however, was not inhibited by hydrogen peroxide but inhibited by sodium azide. The activity in cell extracts from cells grown in iron-rich medium was found to be highly sensitive to hydrogen peroxide and sodium azide. One mol of native enzyme was found to contain 1.1 g-atom of iron and 0.7 g-atom of manganese. The N-terminal amino acid sequence of the purified enzyme was Ala-Tyr-Thr-Leu-Pro-Pro-Leu-Asn-Tyr-Ala-Tyr. The superoxide dismutase of Methylobacillus sp. strain SK1 was found to have antigenic sites identical to those of Methylobacillus glycogenes enzyme. The enzyme, however, shared no antigenic sites with Mycobacterium sp. strain JC1, Methylovorus sp. strain SS1, Methylobacterium sp. strain SY1, and Methylosinus trichosproium enzymes.

• Korean Journal of Ecology and Environment
• /
• v.42 no.1
• /
• pp.95-106
• /
• 2009

The Shihwa constructed wetland was established for the treatment of severely poluted water from Banwoul, Donghwa and Samhwa streams. This study was focused on investigating the structure of epiphytic diatom communities on reed (Phragmites communis) planting area at Banwoul high wetland (3 stations) and low wetland (3 stations) from March to October 2005. The concentration of T-N of inlet stations from the streams were decreased by flowed through the wetland, but the concentration of T-P were increased at outflow part. Epiphytic diatoms on the reed were a total 109 taxa which were composed of 103 species, 5 varieties, 1 unidentified species. The standing crops were rapidly decreasing tendency from spring to autumn but chlorophyll-a concentration were showed a very irregulated changes. Dominant species were 6 taxa which were Achnanthes minutissima in the early investigation, and were changed to the genus Navicula and the genus Nitzschia in the summer. DAIpo were ranged the values of $20.2{\sim}51$, which were mesosaprobic states at the high wetland and ranged the values of $12.4{\sim}52$, which were polysaprobic to mesosaprobic states at the low wetland. TDI were ranged the values of $28.8{\sim}94.5$, which were oligotrophic to eutrophic state at the high wetland and ranged the values of $33.3{\sim}89.7$ which were mesotrophic to eutrophic states at the low wetland. The healthy assessment (DAIpo and TDI) of water ecosystem were showed clean-bad from spring to autumn. These epiphytic diatom communities were determinated by the biological factor such as the growth of reed and the physical factors such as water temperature, light penetration and SS and so on.

• International Journal of Arrhythmia
• /
• 제18권2호
• /
• pp.66-76
• /
• 2017

Background and Objectives: Although an anterior linear ablation is an effective lesion set in radiofrequency catheter ablation (RFCA) for longstanding persistent atrial fibrillation (L-PeAF), its durability for bidirectional block (BDB) is only about 60% at repeat procedure. We hypothesized that changes in electrocardiogram (ECG) may predict an anterior line block state and the clinical outcome of L-PeAF ablation. Subjects and Methods: We studied 304 L-PeAF patients (77% male, $60{\pm}10yrs$), who consistently underwent RFCA Dallas lesion set (circumferential pulmonary vein isolation, posterior box lesion, and anterior line) protocol with subsequent comparison of pre-procedural and post-procedural P wave axes, and one year follow-up (n=205) sinus rhythm (SR) ECGs. Results: 1. P wave axis shifted inferiorly at immediate post-procedure (p<0.001), and was independently correlated with BDB of anterior line (${\ss}=10.4$, 95% confidence interval [CI] 2.79-17.94, p=0.008). 2. The degree of post-procedural inferior shift of P wave axis did not reflect clinical recurrence within one-year (n=205, p=0.923), potentially due to conduction recovery of an anterior line. However, among 160 patients without clinical recurrence within one-year, P wave axis at one-year ECG was independently associated with very late recurrence of AF after one-year (n=160, hazard ratio [HR] 0.98; 95% CI 0.97-0.99, p=0.001), during $45.6{\pm}16.7$ months of follow-up. 3. Among 22 patients who underwent repeat procedures, P wave axis shift was more significant in patients with maintained BDB of an anterior line than in those without (p=0.015). Conclusion: An inferior shift of P wave axis reflects the achievement and the maintenance of an anterior line BDB, and is associated with better long-term clinical outcome after catheter ablation for L-PeAF based on Dallas lesion set.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2018.05a
• /
• pp.318-318
• /
• 2018

충주댐 하류 남한강 본류에서는 매년 봄철 지속되는 가뭄으로 인한 유량감소와 과다한 영양염류 유입으로 인해 부영양화 현상이 빈번히 발생하고 있다. 이에 본 연구에서는 남한강 본류로 유입되는 주요 하천(섬강, 청미천, 양화천, 복하천)을 대상으로 중점관리가 필요한 우심하천 및 주요 오염원을 선별하고자 갈수기 오염부하 특성 및 남한강 본류에 대한 기여율을 분석하였다. 분석 결과, 갈수기 조건에서의 오염부하 및 기여율은 섬강에서 BOD5 852.0 kg/day(41.3%), T-P 59.0 kg/day(56.8%)로 가장 높게 나타났으며, 유량 및 수질 모니터링 기간 내 발생한 점 기저비점 오염부하가 남한강 본류에 미치는 기여율 BOD5 52.8%, COD 52.1%, TOC 53.0%, SS 41.3%, T-N 47.7%, T-P 30.0%, Chl-a 47.8%로 섬강에서 가장 높게 나타났다. 그리고 전국오염원조사 자료를 바탕으로 산정된 수질항목에 대한 오염원별 전체 배출부하 또한 섬강에서 가장 높게 나타났으며, 토지계, 생활계, 축산계 오염원 순으로 높은 비중을 차지하는 것으로 나타났다. 본 연구 결과를 통해 충주댐 하류 남한강 본류 갈수기 수질을 효율적으로 개선하기 위해서는 섬강을 중점적으로 점오염원 관리뿐만 아니라, 기저비점을 통합적으로 관리하기 위한 대책 마련이 필요할 것으로 판단된다. 향후 본 연구의 결과는 극한 가뭄이 지속되는 상황을 고려한다면, 충주댐 하류 남한강 본류 갈수기 수질오염 개선에 활용될 실효성 있는 대책 및 유역관리 정책 수립에 대한 기초 자료로 활용될 수 있을 것이라 사료된다.

• Journal of Fisheries and Marine Sciences Education
• /
• v.24 no.4
• /
• pp.602-608
• /
• 2012

This study was conducted to evaluate the effect of commercial extruded pellet (EP) diet on the growth and water quality compared with a raw fish moist pellet (MP) diet for flounder on field feeding experiments. Three replicate groups of 130 fish per each tank (initial weight of 380 g) were fed one of the EP and Moist pellet (MP) for 7 months. In field feeding experiment, no significant difference was observed in final mean weight of fish fed the experimental EP (1,233 g) and MP (1,237 g), and any noticeable problem caused by feeding the experimental EP was not found during the whole period of feeding trial. Feed efficiency of fish fed the experimental EP was higher than that of MP, and there was no significant difference in survival between the fish fed experimental EP and MP. The concentrations of SS, COD, TN and TP of seawater in tanks of MP groups were 1-5 times higher than those of EP groups. Therefore, these results strongly suggest that diet EP could be developed to replace MP diet for the marketable size of production for flounder without adverse effects on growth performance and water quality.

• Korean Journal of Soil Science and Fertilizer
• /
• v.48 no.1
• /
• pp.64-71
• /
• 2015

Soil contamination with arsenic and heavy metals is a worldwide problem. Main objective of this research was to evaluated effects of reducing heavy metal leaching under reduced soil condition amended with industrial by-products. The contaminated soil was amended with 3% (w/w) of limestone (Ls), steel slag (SS) and acid mine drainage sludge (AMDS). Synthetic acid rain ($H_2SO_4:HNO_3=6:4$, pH 5.5 fixed) was used for feeding solution with flow rate of $0.78{\sim}0.88mL\;min^{-1}$. Results showed that similar pH and EC of leachate was observed in all treatments regardless of applied industrial by-products. However, arsenic concentration of leachate increased when industrial by-products were mixed. Meanwhile, concentration of heavy metal in the leachate decreased from 11.3 to 4.59 mg for Cd, from 92.3 to 7.93 mg for Pb, and from 11,716 to 1,788 mg for Zn via immobilization in soil with AMDS amended, respectively. Overall, application of industrial by-products can be an environmentally-friendly way to remediate soil and(or) leachate contaminated with metal(loid)s in metal mine site.

• Journal of Korean Society on Water Environment
• /
• v.37 no.1
• /
• pp.47-54
• /
• 2021

Aerobic granular sludge (AGS) can be classified as a type of self-immobilized microbial aggregates measuring more than 0.2 mm. It offers the option to simultaneously remove COD, N, and P that occur in different zones inside a granule. Also, AGS is characterized by high precipitability, treatability with high organic loading, and high tolerance to low temperature. In this study, a sequencing batch reactor inoculated with AGS (AGS-SBR) is a new advanced wastewater treatment process that was proven to grow AGS with integrated nutrient removal and low C/N ratio. A pilot plant, AGS-SBR with a capacity of 225 ㎥/d was installed at an S sewage treatment plant in Gyeonggi-do. The results of the operation showed that the water quality of the effluent indicated that the value of BOD5 was 1.5 mg/L, CODMn was 11.4 mg/L, SS was 6.2 mg/L, T-N was 13.2 mg/L, and T-P was 0.197 mg/L, and all of these values reliably satisfied an effluent standard (I Area). In winter, the T-N treatment efficiency at a lower temperature of less than 11℃ also showed reliability to meet the effluent standard of the I Area (20 mg/L or less). Analysis of microbial community in AGS showed a higher preponderance of beneficial microorganisms involved in denitrification and phosphorus accumulation compared with activated sludge. The power consumption and sludge disposal cost were reduced by 34.7% and 54.9%, respectively, compared to the domestic SBR type sewage treatment plant with a processing capacity of 1,000 ㎥/d or less.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.33 no.2
• /
• pp.151-157
• /
• 2019

Academic burn-out is a state of emotional, and physical exhaustion caused by prolonged stress over the course of the semester and academic year. It is composed of exhaustion, inefficacy, and cynicism. The purpose of this study was to reveal that the academic burn-out would be significantly different between early and the end stage of semester, and the sub pattern would be different according to Sasang constitution among the healthcare college students. The first survey was conducted in early March 2018 and the second one was done in the end of June 2018. Total 386 questionnaires concerning KS-15 of Sasang Constitution, MBI-SS of academic burn-out and general characteristics such as drinking, smoking and so on were collected and 9 of them were excluded, because of insufficient answers. The academic burn-out score significantly increased from 56 in early stage of semester to 63 in the end stage of semester (p<.001). In sub-pattern analysis, the exhaustion score significantly increased from 21.3 to 26.7 (p<.001), and cynicism score increased from 15.4 to 17 (p<.001). The cynicism score of drinking alcohol group ($16.21{\pm}5.572$) was higher than the non-drinking alcohol group ($14.62{\pm}5.859$), and the exhaustion score of smoking group ($23.75{\pm}6.942$) was significantly higher than that of non-smoking group ($21.05{\pm}6.697$) in early stage of semester. The exhaustion score of female ($27.47{\pm}5.762$) was higher than that of male ($26.02{\pm}6.536$) (p=.024), and the inefficacy score of Taeeumin ($20.13{\pm}5.244$) was significantly higher than that of Soyangin ($18.15{\pm}5.588$) in the end stage of semester. The healthcare college student experiences academic burn-out, and there is significantly different according to gender and SC in sub-pattern. Further study is needed to manage the academic burn-out according gender and Sasang constitution.

• Journal of Ocean Engineering and Technology
• /
• v.33 no.3
• /
• pp.214-221
• /
• 2019

A series of model tests was performed to evaluate the survivability of an articulated tower-type buoy structure under harsh environmental conditions. The buoy structure consisted of three long pipes, a buoyancy module, and top equipment. The scale model was made of acrylic pipe and plastic with a scale ratio of 1/22. The experiments were carried out at the ocean engineering basin of KRISO. The performance of the buoy structure was investigated under waves only and under combined environmental conditions from sea state (SS) 5 to 7. A nonlinear time-domain numerical simulation was conducted using the mooring analysis program OrcaFlex. The survivability of the buoy was analyzed based on three factors: the pitch motion, submergence of the top structure, and anchor reaction force. The model test results were directly compared to the results of numerical simulations. The effects of the sea state and combined environment on the performance of the buoy structure were investigated.

• Proceedings of the SCSK Conference
• /
• 2003.09a
• /
• pp.501-519
• /
• 2003

Silk Sericin(SS) is a natural protein extracted from cocoon of bombix mori and shows moisturizing effect to the skin due to a number of hydroxyl groups in the structure. But its application to cosmetics is limited due to its poor solubility in water. In order to solve this drawback and expand its application to cosmetics, polyethyleneglycol(PEG) was conjugated with sericin by reacting activated polyethyleneglycol(ActPEG). Reaction site of sericin is tyrosine residue, which was determined by using $^1$H-NMR. Random coil structure of sericin was transformed to beta-sheet structure by conjugating polyethyleneglycol. It was confirmed that melting point of sericin-PEG conjugate was lowered compared to that of each sericin and PEG due to the interaction between sericin and PEG in crystalline structure. Self-assembled sericin-PEG nanoparticle was obtained by dialyzing with alcohol solution of sericin-PEG conjugate against water. The particle is spherical and has 200-400nm of size. The moisturizing ability of sericin-PEG nanoparticle was much higher than that of sericin itself. Incorporation of vitamin A into sericin-PEG nanoparticle was carried out by diafiltration method. The content of incorporated Vitamin A in sericin-PEG nanoparticle was 8.9 wt％. Releasing behaviour of vitamin A incorporated into nanoparticle was tested in phosphate buffer, pH 7.4 at 37$^{\circ}C$. and half-life of Vitamin A release was 43hrs. Sericin-PEG nanoparticle exhibited higher moisturing effect than sericin itself and distilled water, respectively. No toxicity and irritation were observed in animal tests. It can be expected that the self-assembled sericin-PEG nanoparticle can be developed for cosmetics.