• Title/Summary/Keyword: SS concentration

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Feeding pattern, biochemical, anthropometric and histological effects of prolonged ad libitum access to sucrose, honey and glucose-fructose solutions in Wistar rats

  • Virgen-Carrillo, Carmen Alejandrina;Moreno, Alma Gabriela Martinez;Rodriguez-Gudino, Juan Jose;Pineda-Lozano, Jessica Elizabeth
    • Nutrition Research and Practice
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    • v.15 no.2
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    • pp.187-202
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    • 2021
  • BACKGROUND/OBJECTIVES: The exposure to sucrose in rats has mimic abnormalities attributed to metabolic syndrome (MetS). The effects of honey bee and "free" glucose and fructose, have not been explored in this context. The aim was to expose Wistar rodents to sucrose solution (SS), honey solution (HS) and fructose/glucose solution (GFS) at 30% to assess their effects. SUBJECTS/METHODS: HS (n = 10), SS (n = 10) and GFS (n = 10) groups were formed. Solutions were ad libitum along 14-weeks. RESULTS: Between solutions consumptions, honey was significantly 42% higher (P = 0.000), while similar consumption was observed among GFS and SS. The feeding pattern of HS consumption was irregular along experiment; while the food intake pattern showed the similar trend among groups along time. Non statistical differences were obtained in any biochemical and anthropometric measure, however, a higher concentration of leptin (721 ± 507 pg/mL), lower concentration of total cholesterol (TC; 48.87 ± 2.41 mg/100 mL), very low density lipoprotein (VLDL; 16.47 ± 6.55 mg/100 mL) and triglycerides (82.37 ± 32.77 mg/100 mL) was obtained in SS group. For anthropometric values, HS showed less total adipose tissue (AT; average 26 vs. 31-33 g) and adiposity index (average 6.11 vs. 7.6). Due to sugar-sweetened beverages consumption increases the risk for the development of chronic diseases; correlations between fluid intake and anthropometric and biochemical parameters were assessed. A moderate correlation was obtained in groups with the weight of total AT and solution intake; for the weight gain in GFS group and for triglycerides in HS and GFS. The highest hepatic tissue damage was observed in SS group with multiple intracytoplasmic vacuoles, atypia changes, moderate pleomorphism and hepatocellular necrosis. CONCLUSIONS: In spite of the significantly higher consumption of HS, biochemical, anthropometrical and histological effects were not remarkably different in comparision to other sweeteners.

Characterization of the Water Soluble Organic Fraction Extracted from a Sewage Sludge Amended Soil (Sewage Sludge를 시용(施用)한 토양(土壤)에서 추출(抽出)한 수용성유기물(水溶性有機物)의 화학구조적(化學構造的) 특성(特性))

  • Lim, Hyungsik;Volk, V.V.;Baham, J.
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.1
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    • pp.38-49
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    • 1985
  • The water soluble organic fractions (WSOF) from an agricultural soil (W), a soil treated with sludge for 6 years ($WS_6$), a sludge-soil mixture incubated for one week ($WS_1$), and sewage sludge (SS) were extracted, purified, and characterized by elemental analysis, functional group determinations, infrared, UV-visible, and proton nuclear magnetic resonance spectrosocpy. The SS was characterized by higher organic H, N, and P contents, a higher H/C ratio, and a lower C/N ratio than W. Total acidity carboxyl and phenolic hydroxyl group contents were generally highest in SS, intermediate in $WS_6$ and $WS_1$, and lowest in W. Overall aromatic character and aromatic carboxyl group contents were highest in W, and lowest in SS. Aliphatic proton, aliphatic carboxyl, and phenolic hydroxyl group contents were highest in SS, and lowest in W. Protein decomposition products were the pronounced components in SS, and decreased in concentration as the sludge component in the mixtures decreased. The $^1H$-NMR spectra suggested that the SS-protons were bound to a wider range of functional groups than W-protons. Structural complexities around the aromatic protons followed the following order: SS>$WS_1$>$WS_6$>W.

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미세조류의 Methane 발효특성

  • 강창민;최명락
    • Microbiology and Biotechnology Letters
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    • v.24 no.5
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    • pp.597-603
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    • 1996
  • This study was carried out to examine degradation characteristics of microalgae Chlorella vulgaris in methane fermentation. We measured COD and VS reduction, gas and methane productivity, VFA (volatile fatty acid), respectively. Then we calculated material balance and hydrolysis rates in soluble and solid material. The substrate concentration was controlled from 14 gCOD$_{cr}$/l to 64 gCOD$_{cr}$/l in batch cultures, and HRT (hydraulic retention time) controlled from 2 days to 30 days in continuous experi- ments. The results were as follows. In batch culture, accumulated gas productivity increased with the increase of the substrate concentration. The SS and VSS was removed all about 30% increase of substrate concentration and the most of the degradable material removed during the first 10 days. The curve of gas and methane production rate straightly increased until substrate concentration is 26 gCOD$_{cr}$/l. In continuous culture experiments, the removal rates at HRT 10days were 20% for total COD and TOC, respectively. At longer HRT, there was no increase in the removal efficiency. At HRT 15 days, the removal rates were 30% for SS and VSS, respectively. Soluble organic materials were rapidly degraded, and so there was no accumulated. Soluble COD concentration was not increase regardless of HRT-increasing. That meaned the hydrolysis was one of the rate-limiting stage of methane fermentation. The first-order rate constants of hydrolysis were 0.23-0.28 day$^{-1}$ for VSS, and 0.07-0.08 day$^{-1}$ for COD.

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Development of Ocean Environmental Algorithms for Geostationary Ocean Color Imager (GOCI) (정지궤도 해색탑재체(GOCI) 해수환경분석 알고리즘 개발)

  • Moon, Jeong-Eon;Ahn, Yu-Hwan;Ryu, Joo-Hyung;Shanmugam, Palanisamy
    • Korean Journal of Remote Sensing
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    • v.26 no.2
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    • pp.189-207
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    • 2010
  • Several ocean color algorithms have been developed for GOCI (Geostationary Ocean Color Imager) using in-situ bio-optical data sets. These data sets collected around the Korean Peninsula between 1998 and 2009 include chlorophyll-a concentration (Chl-a), suspended sediment concentration (SS), absorption coefficient of dissolved organic matter ($a_{dom}$), and remote sensing reflectance ($R_{rs}$) obtained from 1348 points. The GOCI Chl-a algorithm was developed using a 4-band remote sensing reflectance ratio that account for the influence of suspended sediment and dissolved organic matter. The GOCI Chl-a algorithm reproduced in-situ chlorophyll concentration better than the other algorithms. In the SeaWiFS images, this algorithm reduced an average error of 46 % in chlorophyll concentration retrieved by standard chlorophyll algorithms of SeaWiFS. For the GOCI SS algorithm, a single band was used (Ahn et al., 2001) instead of a band ratio that is commonly used in chlorophyll algorithms. The GOCI $a_{dom}$ algorithm was derived from the relationship between remote sensing reflectance band ratio ($R_{rs}(412)/R_{rs}(555)$) and $a_{dom}(\lambda)$). The GOCI Chl-a fluorescence and GOCI red tide algorithms were developed by Ahn and Shanmugam (2007) and Ahn and Shanmugam (2006), respectively. If the launch of GOCI in June 2010 is successful, then the developed algorithms will be analyzed in the GOCI CAL/VAL processes, and improved by incorporating more data sets of the ocean optical properties data that will be obtained from waters around the Korean Peninsula.

Regional Variation and Discharge Characteristics of Stream Water Quality and Heavy Metals Around the Shihwa Lake Basin (시화호 유역 하천수 일반수질 및 중금속의 변화 및 유출 특성 연구)

  • Jeong, Hyeryeong;Kim, Kyung-Tae;Kim, Eun-Soo;Lee, Seung-Yong;Ra, Kongtae
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.20 no.2
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    • pp.76-83
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    • 2017
  • In order to investigate the characteristics of water quality and heavy metals in Shihwa Lake, concentration comparisons according to land use types and mass discharge through streams were carried out. Based on classification for streams according to land use types, the concentration of DO, SS, POC, $NO_3$ and Chl-a showed higher concentrations in agricultural areas than in industrial and urban areas. However, COD, DIN, DIP, TN, TP, DOC, TOC and heavy metal concentrations showed relatively high values in industrial areas. The concentrations of water qualities and heavy metals were relatively high in March to May and the concentration decreased in summer seasons (July-August). The averages of stream discharges of water qualities were 1,172 kg/d for SS, 151 kg/d for TN, 11.1 kg/d for TP and 389 kg/d for TOC, respectively. The agricultural area, Jangjunbo(S8), accounted for 47.5%(TP)~75.1%(SS) in the total stream discharges. Cu, Zn and Cd in stream waters were found to be more than 92% of the total discharges in industrial area, showing descending order of Zn>Cu>Ni>Pb>Co>Cd.

Effects of Disinfectant Concentration, pH, Temperature, Ammonia, and Suspended Solids on the Chlorine Disinfection of Combined Sewer Overflow (소독제 농도, pH, 온도, 암모니아 농도, 부유물질이 강우 월류수 염소 소독에 미치는 영향)

  • Kim, Sang-Hyoun
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.10
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    • pp.685-690
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    • 2014
  • The treatment of combined sewer overflow (CSO) is one of potential concerns in domestic wastewater treatment in Korea due to the pre-announce of CSO regulations. This work investigated the effects of disinfectant (NaOCl) concentration (0.11 to 4.0 mg $Cl_2/L$), pH (6.5 to 8.0), temperature (15 to $25^{\circ}C$), ammonia (10 to 41 mg N/L), and suspended solids (91 to 271 mg SS/L) on the chlorine disinfection of CSO. The effect of NaOCl concentration on the pseudo-$1^{st}$ order reaction rate for total coliform inactivation was described well with a saturation-type model with the half-velocity constant of 1.212 mg/L. The total coliform inactivation reaction rate decreased with SS and pH, and increased with temperature. Ammonia in the examined range did not affect the disinfection kinetics. A chlorine contact tank with the injection chlorine level of 1 mg $Cl_2/L$ and the hydraulic retention time of 1.25 min is estimated to reduce total coliform from $1{\times}10^5MPN/mL$ to 1,000 MPN/mL at 271 mg SS/L, $15^{\circ}C$, and pH 8.0. Chlorine would be a proper option for the disinfection of CSO.

Determination of Representative Long-term Water Quality Parameters of the Streams in Estuarine Lake (하구호 유입하천의 유역특성에 따른 장기 대표 수질절정)

  • Kim, Young-Chul;Lee, Dong-Ryul;Hwang, Gil-Son
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.3
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    • pp.262-272
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    • 2005
  • This study was undertaken to obtain design concentrations for the natural system treating stream water flowing into estuarine lake(Sapgyo). This lake has three major, so called, national-class streams: one is Gokgyo flowing through two medium-size municipal areas and the others are Muhan and Sapgyo placed in mostly rural area nested with some small towns. The results of three year's investigation showed big changes in water quality during the period between dry and wet seasons. Nitrogen concentration usually decreases as the flow rate increases. The change of phosphorus concentration in Gokgyo was the same pattern as nitrogen, but in other two streams it was inversely related with flow rates. This is probably due to the chemical property of the particles washed out from the different stream basins during wet season. It was found that about 40 to 60% of the total nitrogen are in soluble form and on average, 35% of the total phosphorus consists of dissolved-P. Representative concentration of these streams has to be separately determined based on dry and wet season. TDS and SS could be indicating parameters useful to depict and characterize a change of water quality with respect to flow rate because their measurement is easy and also includes a small analytical error. TDS values measured during dry season are related with high nitrogen concentration while during wet season, SS values effectively reflect a high concentration of phosphorus. For design purpose, cumulative concentration distribution graph presented in this paper can be directly or indirectly applied to other streams through a brief comparative and verificative study.

The Movement Characteristics of Suspended Solid(SS) in Harbor Construction(II) - The Landfill Construction and The Grab Dredging Construction (항만공사시 부유토사의 거동 특성에 관한 연구(II) - 매립 및 준설공사시 -)

  • Lee Ji Wang;Maeng Jun Ho;Cho Kwang Woo;Yang Gwon Weol;Kim Young Seup
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.6 no.2
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    • pp.46-53
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    • 2003
  • In order to analyze the movement characteristics of suspended solid(SS) in harbor construction, we investigate the generation and movement processes of the SS for the landfill construction by direct casting method and the grab dredging construction which is the most frequent process in harbor construction. We find that the SS is generated into a very high concentration right after the direct casting of landfill soil and continued up to 60 minutes in the landfill construction using the direct casting method with dredged materials by a ship of 700-tonnage. In the grab dredging construction, the SS is generated in a high density of concentration near at its source regardless of water depth, formed belt and diffused up to 700m along the trajectory of tidal current. Based on the result of the present study, it is recommended for the mitigation of SS generation that the silt protector be deployed near at construction site close enough to block the diffusion of SS and the body length of silt protector be long enough to mitigate the SS diffusion in the bottom layer.

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Single-stranded DNA Enhances the Rate of Product Release During Nucleotide Hydrolysis Reaction by T7 DNA Helicase

  • Kim, Dong-Eun;Jeong, Yong-Joo
    • Bulletin of the Korean Chemical Society
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    • v.27 no.10
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    • pp.1618-1622
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    • 2006
  • Bacteriophage T7 gp4A' is a ring-shaped hexameric DNA helicase that catalyzes duplex DNA unwinding using dTTP hydrolysis as an energy source. To investigate the effect of single-stranded DNA (ssDNA) on the kinetic pathway of dTTP hydrolysis by the T7 DNA helicase complexed with ssDNA, we have first determined optimal concentration of long circular M13 single-stranded DNA and pre-incubation time in the absence of $Mg^{2+}$ which is necessary for the helicase-ssDNA complex formation. Steady state dTTP hydrolysis in the absence of $Mg^{2+}$ by the helicase-ssDNA complex provided $k_{cat}$ of $8.5\;{\times}\;10^{-3}\;sec^{-1}$. Pre-steady state kinetics of the dTTP hydrolysis by the pre-assembled hexameric helicase was monitored by using the rapid chemical quench-flow technique both in the presence and absence of M13 ssDNA. Pre-steady state dTTP hydrolysis showed distinct burst kinetics in both cases, indicating that product release step is slower than dTTP hydrolysis step. Pre-steady state burst rates were similar both in the presence and absence of ssDNA, while steady state dTTP hydrolysis rate in the presence of ssDNA was much faster than in the absence of ssDNA. These results suggest that single-stranded DNA stimulates dTTP hydrolysis reaction by T7 helicase by enhancing the rate of product release step.

Anti-obesity Effect of Steamed Soybean and Fermented Steamed Soybean in High-fat Diet-induced Obese ICR Mice

  • Seo, Hye Rin;Lee, Ah Young;Cho, Kye Man;Cho, Eun Ju;Kim, Hyun Young
    • Natural Product Sciences
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    • v.23 no.1
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    • pp.61-68
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    • 2017
  • This study was performed to investigate the ameliorating effects of steamed soybeans (SS) and fermented SS (FSS) on lipid metabolism in high-fat diet-induced obese mice. ICR mice were divided into four groups and given the following different diets: normal diet (ND), high-fat diet (HFD), HFD with 1% SS (HFD + SS), and HFD with 1% FSS (HFD + FSS). After 14 weeks, the body weight gain was higher in the HFD group compared with the ND group but lower in the HFD + FSS group compared with the HFD group. Plasma levels of triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were significantly higher in the HFD group compared to the ND group, but lower in the HFD + SS and HFD + FSS groups compared with the HFD group. In addition, leptin concentration in plasma was lower in the groups fed HFD + SS and HFD + FSS compared with the HFD group. The accumulation of hepatic TG and TC was significantly inhibited in the HFD + SS and HFD + FSS groups. Furthermore, SS and FSS attenuated lipid peroxidation and nitric oxide formation in the liver induced by the high-fat diet. These results suggest that soybeans, especially FSS, may be useful in preventing obesity-induced abnormalities in lipid metabolism.