• Journal of the Korean Society of Safety
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• v.3 no.1
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• pp.21-29
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• 1988

Conventional pwm switching power supply have the disadvantage some aspects of size, light weight, noise and system stability. High frequency Series Resonant Converter (SRC), described in this paper, almost improve above disadvantages. We use the state plane technique as analysis method. This technique is powerful tool which can clearly analyze the peak stress of the state variables inside the converter, Here, we can define each operation mode from frequency ratio Fsn, switching frequency to resonant frequency, and we analyze the output performance in each operation mode. To verify the theoretical analysis, we compose the actual converter, and the experimental results are compared with analysis.

• Proceedings of the KIPE Conference
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• 2018.11a
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• pp.201-202
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• 2018

본 논문에서는 넓은 범위의 입력전압에서의 빠른 응답속도를 가진 고승압 양방향 컨버터를 제안한다. 제안하는 시스템은 2대의 Buck컨버터와 SRC(Seris Resonant Covnerter)로 구성된 3-stage 구조이며, 고승압이 가능하고 넓은 입력전압 범위에서 고효율을 성취할 수 있다. 또한 제안하는 컨버터는 모든 전압범위에서 빠른 응답속도로 제어를 하며 CC(Constant Current), CV(Constant Voltage), CP(Constant Power) 및 Pulse 동작을 수행한다. 제안하는 양방향 컨버터의 1.8kW급 시작품으로 실험을 통하여 타당성을 검증하였고, 최고효율 96%, 정격효율 90.8%를 달성하였다.

• BMB Reports
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• v.32 no.2
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• pp.119-126
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• 1999

The SH3 domain of PLC-${\gamma}1$ has been known to induce DNA synthesis. However, little is known about the putative effector proteins that associate with the domain. In this report, we provide evidence that the SH3 domain of PLC-${\gamma}1$ associates with Shc, which has been implicated in the activation of p21Ras in response to many growth factors. The association between Shc and PLC-${\gamma}1$ is enhanced either by v-Src-induced transformation or EGF-stimulation in vivo and in vitro. Furthermore, from transient expression studies with COS-7 cells, we show that the SH3 domain of PLC-${\gamma}1$ is required for association with Shc in vivo, whereas tyrosyl phosphorylation of PLC-${\gamma}1$ is not. Taken together, we suggest that Shc might be involved in the PLC-${\gamma}1$-mediated signaling pathway.

• Animal cells and systems
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• v.14 no.3
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• pp.155-160
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• 2010

Extracellular signal-regulated kinases 1/2 (ERK1/2) play important roles in a variety of biological processes including cell growth and differentiation. We have previously reported that GAR-3 activates ERK1/2 via phospholipase C and protein kinase C, presumably through pertussis toxin (PTX)-insensitive Gq proteins, in Chinese hamster ovary (CHO) cells. Here we provide evidence that GAR-3 also activates ERK1/2 through PTX-sensitive G proteins, phosphatidylinositol 3-kinase (PI 3-kinase), and Src family kinases in CHO cells. We further show that in human embryonic kidney (HEK293) cells, epidermal growth factor receptor and Ras are required for efficient ERK1/2 activation by GAR-3. Taken together, our data indicate that GAR-3 evokes ERK1/2 activation through multiple signaling pathways in cultured mammalian cells.

• Journal of Microbiology and Biotechnology
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• v.22 no.3
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• pp.378-384
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• 2012

In aquatic invertebrates, particularly marine gastropods, organotin compounds induce irreversible sexual abnormality in females, which is termed imposex, at very low concentrations. Organotin compounds are agonists for nuclear receptors such as RXRs and $PPAR{\gamma}$. However, the imposex phenomenon has not been reported to act as an antagonist on estrogen receptors in other species, including vertebrates and invertebrates. In order to gain insights into the antagonistic activity of organotin compounds on estrogen receptors (ERs), we examined the inhibitive effect of these compounds on estradiol-dependent ${\beta}$-galactosidase activity using the yeast two-hybrid detection system consisting of a combination of the human estrogen receptor ($hER{\beta}$) ligand-binding domain and the co-activator steroid receptor co-activator-1 (SRC1). Tributyltin-hydroxide (TBT-OH) and triphenyltin-chlorine (TPT-Cl) exhibited an inhibitive effect on $E_2$-dependent transcriptional activity, similar to antagonistic chemicals such as 4-hydroxytamoxifen (OHT) or ICI 182,780, at a very low concentration of $10^{-14}$ M TBT or $10^{-10}$ M TPT, respectively. The yeast growth and transcriptional activity with transcriptional factor GAL4 did not exhibit any effect at the tested concentration of TBT or TPT. Moreover, the yeast two-hybrid system using the interaction between p53 and the T antigen of SV40 large did not describe any effect at the tested concentration of OHT or ICI 182,780. However, the interaction between p53 and T antigen was inhibited at a TBT or TPT concentration of $10^{-9}$ M, respectively. These results indicate that TBT and TPT act as inhibitors of ER-dependent reporter gene transcriptional activation and of the interaction between $hER{\beta}$ LBD and the co-activator SRC1 in the yeast two-hybrid system. Consequently, our data could partly explain the occurrence of organotin compound-induced imposex on the endocrine system of mammals, including humans.

• BMB Reports
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• v.43 no.3
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• pp.199-204
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• 2010

The protein p104 was first isolated as a binding partner of the Src homology domain of phospholipase C$\gamma$1, and has been shown to associate with p85$\alpha$, Grb2. The ectopic expression of p104 reduced cellular growth rate, which was also achieved with the overexpression of only the proline-rich region of p104. The proline-rich region of p104 has been found to inhibit the colony formation of platelet-derived growth factor BB-stimulated NIH3T3 cells and MCF7 cancer cells on soft agar. Mutagenesis analysis showed that the second and third proline-rich regions are essential for growth control, as well as for interaction with p85$\alpha$. Overexpression of p104 increased the level of the cyclin-dependent kinase inhibitor, $p27^{Kip1}$, and inhibited the activity of phosphoinositide 3-kinase (PI3K). In summary, p104 interacts with p85$\alpha$ and is involved in the regulation of $p27^{Kip1}$ expression for the reduction of cellular proliferation.

• The Korean Journal of Nuclear Medicine
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• v.33 no.1
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• pp.1-10
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• 1999

Cancer cells are known to show increased rates of glycolysis metabolism. Based on this, PET studies using F-18-fluorodeoxyglucose have been used for the detection of primary and metastatic tumors. To account for this increased glucose uptake, a variety of mechanisms has been proposed. Glucose influx across the cell membrane is mediated by a family of structurally related proteins known as glucose transporters (Gluts). Among 6 isoforms of Gluts, Glut-1 and/or Glut-3 have been reported to show increased expression in various tumors. Increased level of Glut mRNA transcription is supposed to be the basic mechanism of Glut overexpression at the protein level. Some oncogens such as src or ras intensely stimulate Glut-1 by means of increased Glut-1 mRNA levels. Hexokinase activity is another important factor in glucose uptake in cancer cells. Especially hexokinase type II is considered to be involved in glycolysis of cancer cells. Much of the hexokinase of tumor cells is bound to outer membrane of mitochondria by the porin, a hexokinase receptor. Through this interaction, hexokinase may gain preferred access to ATP synthesized via oxidative phosphorylation in the inner mitochondria compartment. Other biologic factors such as tumor blood flow, blood volume, hypoxia, and infiltrating cells in tumor tissue are involved. Relative hypoxia may activate the anaerobic glycotytic pathway. Surrounding macrophages and newly formed granulation tissue in tumor showed greater glucose uptake than did viable cancer cells. To expand the application of FDG PET in oncology, it is important for nuclear medicine physicians to understand the related mechanisms of glucose uptake in cancer tissue.

• Microbiology and Biotechnology Letters
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• v.21 no.1
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• pp.59-65
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• 1993

We isolated Actinomycetes strain GMT 1155 and purified the active compound, MT 1155, on the morphological reversion of ts/NRK cell from the isolate. MT 1155 was identified as toyocamycin having antifungal and antitumor activities from physico-chemical properties and UV, IR, $^1H$-NMR, $^13C$-NMR and mass spectrum. MT 1155 showed the morphologically reversional activity on ts/NRK cell and the cytotoxicity on CTLL cell at the final concentrations of 1.7 JlM and 0.2 11M, respectively and its $IC_{50}$ value on protein kinase A enzyme was 2.3 $\mu$M. Also it had strong antifungal activity against several pathogenic fungi but not antibacterial activity. And it did not inhibit both protein kinase C activity and the bleb-formation of K562 cell induced by phorbol esters.

• Journal of Korean Society of Steel Construction
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• v.29 no.1
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• pp.25-36
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• 2017

In the 1994 Northridge earthquake, connection damage initiated from the beam bottom flange was prevalent. The presence of a concrete slab and resulting composite action was speculated as one of the critical causes of the prevalent bottom flange fracture. In this study, four seismic retrofit schemes are proposed in order to salvage welded steel moment connections with composite floor slabs in existing steel moment frames. Because top flange modification of existing beams is not feasible due to the presence of a concrete floor slab, three schemes of bottom flange modification by using welded triangular or straight haunches or RBS(reduced beam section), and beam web strengthening by attaching heavy shear tab were cyclically tested and analyzed. Test results of this study show that haunch and web-strengthened specimens can eliminate the detrimental effect caused by composite action and ensure excellent connection plastic rotation exceeding 5% rad. Design recommendations for each retrofit scheme together with supplemental numerical studies are also presented.

• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.1
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• pp.7-16
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• 2015

The transmitter of quadrature multiplexed GMSK (QM-GMSK) is composed of two quadrature multiplexed GMSK modulators. QM-GMSK has a slightly increased spectrum main lobe compared with $Q^2PSK$ or QMSK, but it has highly suppressed side lobes. As a result, practical spectrum efficiency of QM-GMSK is achieved. By replacing the baseband elementary pulses of QM-GMSK with their approximate, the squared sinusoid of half-period, offset-$Q^2PSK$($OQ^2PSK$) is obtained. The $OQ^2PSK$ signal has similar spectral properties to QM-GMSK. The transmitter of $OQ^2PSK$ can be simply implemented without the Gaussian lowpass filter, which is required in QM-GMSK transmitter. In this paper, we propose an overlapped pulse shaping in $OQ^2PSK$ with RC(raised-cosine) or SRC(squared raised-cosine) pulses of length longer than the symbol period. Power spectrum of the proposed modulation scheme exhibits further suppressed side lobes, hence enhanced spectrum efficiency is obtained. Simulation results indicate that BER performance of the proposed scheme is comparable to that of $OQ^2PSK$.