• BMB Reports
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• 제31권4호
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• pp.370-376
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• 1998

Src-Homology 2 (SH2) domains have a capacity to bind phosphotyrosine-containing sequence context and play essential roles in various cellular signaling pathways. Due to the specific nature of the binding between SH2 domains and their counterpart proteins, inhibitors of SID domain binding have drawn extensive attention as a potential candidate for therapeutic agents. Here, we describe the binding assay system to screen for the ligands or blockers of the SH2 domains with an emphasis on the $p56^{lck}$ SH2 domain. In our assay system, SID domains expressed and purified as fusion proteins to Glutathione-S-transferase (GST) were covalently attached to 96-well microtitre plates through amide bond formation, which were subsequently allowed to bind the biotinylated phosphotyrosine (pY)containing synthetic pep tides. The binding of biotinylated pY peptides was detected by the horseradish peroxidase (HRP)-conjugated streptavidin. Using the various combinations of SH2 domain-pY peptides, we observed that: (1) The binding of pY-peptides to its counterpart SH2 domain is concentration-dependent and saturable; (2) The binding is highly specific for a particular combination of SH2 domain-pY peptide pair; and (3) The binding of Lck SH2-cognate pY-peptides is specifically competed by the nonbiotinylated peptides with expected relative affinity. These results indicate that the established assay system detects the SH2-pY peptide interaction with reproducible sensitivity and specificity and is suitable for screening the specific inhibitors of $p56^{lck}$ SH2 function.

• 한국강구조학회 논문집
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• 제25권6호
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• pp.635-647
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• 2013

PSRC 기둥은 앵글을 콘크리트에 매입시킨 기둥으로, 단면의 외곽 코너에 배치되는 앵글이 기둥의 휨-압축에 저항한다. 본 연구에서는 KBC 2009에 따라 압축력을 가한 기둥에 대하여 횡방향 반복가력 실험을 통하여 내진성능을 검증하였다. 기둥 종류, 연속후프근 적용, 앵글에 스터드 적용을 실험 변수로 고려하였으며, 2/3 스케일을 갖는 한 개의 SRC 합성기둥과 세 개의 PSRC 합성기둥을 실험하였다. 실험결과, KBC 2009로 예측한 PSRC 합성기둥의 하중재하능력은 실험결과와 잘 일치하였으며, 변형능력과 에너지 소산에 있어서 우수한 성능을 보여주었다. PSRC 합성기둥은 반복하중으로 인한 콘크리트 피복 탈락 이후, 앵글 및 주철근의 좌굴에 의해 실험체의 하중재하능력이 감소하였다. 특히, 연속후프근을 적용한 PSRC 합성기둥은 앵글의 조기 국부좌굴이 억제되어 기존 PSRC 합성기둥에 비해 연성능력이 향상되었다.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1286-1294
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• 2004

In a previous study by the current authors, hepatocellular carcinoma (HCC) was determined to be epidemiologically sex-dependent, and the incidence and multiplicity of HCC found to decrease in estradiol-3 benzoate (EB)-treated F344 rats. Therefore, to ascertain the anticancer mechanism of EB, a commercially available cDNA microarray, with a total of 14,815 cDNA rat gene clones, was used to determine the differentially expressed genes in nontreated livers, EB-treated livers, and diethynitrosolamine (DEN)-induced HCC. In the sequenced experiment, a total of 85 genes were differentially expressed at either two or more times the rate of the normal expression, where 33 genes were downregulated by EB, and 52 genes upregulated. Candidate genes were selected according to significant changes observed in the mRNA expression in the EB-treated livers compared with the nontreated livers, then these genes were filtered according to their different expression patterns in the DEN-induced tumors compared to the estrogen-treated livers. To confirm the microarray data, a real-time PCR analysis was performed for ten selected genes: the H-ras revertant protein 107 (H­rev107), insulin-like growth factor binding protein (lOFBP), parathyroid hormone receptor (PI'HR), SH3 domain binding protein (SH3BP), metallothionein, src-suppressed C-kinase substrate (SSeCK) gene, phosphodiesterase I, CD44, epithelial membrane protein 3 (EMP3), and estrogen receptor a (ERa). The SSeCK and phosphodiesterase I genes were both upregulated in the DEN-induced hepatocarcinomas, yet their possible carcinogenic functions remain unknown. Meanwhile, the other genes were downregulated, including the genes related to growth regulation (IOFBP, H-revI07, ER$\alpha$), adipogenesis inhibition (PTHR), and tumor suppression (metallothionein).

• The Korean Journal of Physiology and Pharmacology
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• 제21권3호
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• pp.345-352
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• 2017

Since previous studies have reported that hydroquinone (HQ) exerted immunosuppressive and anti-inflammatory activity, various HQ derivatives have been synthesized and their biological activities investigated. In this study, we explored the anti-inflammatory activity of JS-III-49, a novel HQ derivative, in macrophage-mediated inflammatory responses. JS-III-49 suppressed the production of the inflammatory mediators nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) and down-regulated the mRNA expression of the inflammatory enzymes cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) as well as the expression of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-$1{\beta}$ without cytotoxicity in LPS-stimulated RAW264.7 cells. JS-III-49 inhibited nuclear translocation of the $NF-{\kappa}B$ transcription factors p65 and p50 by directly targeting Akt, an upstream kinase of the $NF-{\kappa}B$ pathway, in LPS-stimulated RAW264.7 cells. However, JS-III-49 did not directly inhibit the kinase activities of Src and Syk, which are upstream kinases of Akt, in LPS-stimulated RAW264.7 cells. Moreover, JS-III-49 suppressed the nuclear translocation of c-Fos, one of the components of AP-1, by specifically targeting p38, an upstream mitogen-activated protein kinase (MAPK) in the AP-1 pathway in LPS-stimulated RAW264.7 cells. These results suggest that JS-III-49 plays an anti-inflammatory role in LPS-stimulated macrophages by targeting Akt and p38 in the $NF-{\kappa}B$ and AP-1 pathways, respectively.

• Steel and Composite Structures
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• 제22권2호
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• pp.449-472
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• 2016

This paper studies the seismic behavior of reinforced concrete (RC) walls with encased cold-formed and thin-walled (CFTW) steel truss, which can be used as an alternative to the conventional RC walls or steel reinforced concrete (SRC) composite walls for high-rise buildings in high seismic regions. Seven one-fourth scaled RC wall specimens with encased CFTW steel truss were designed, manufactured and tested to failure under reversed cyclic lateral load and constant axial load. The test parameters were the axial load ratio, configuration and volumetric steel ratio of encased web brace. The behaviors of the test specimens, including damage formation, failure mode, hysteretic curves, stiffness degradation, ductility and energy dissipation, were examined. Test results indicate that the encased web braces can effectively improve the ductility and energy dissipation capacity of RC walls. The steel angles are more suitable to be used as the web brace than the latticed batten plates in enhancing the ductility and energy dissipation. Higher axial load ratio is beneficial to lateral load capacity, but can result in reduced ductility and energy dissipation capacity. A volumetric ratio about 0.25% of encased web brace is believed cost-effective in ensuring satisfactory seismic performance of RC walls. The axial load ratio should not exceed the maximum level, about 0.20 for the nominal value or about 0.50 for the design value. Numerical analyses were performed to predict the backbone curves of the specimens and calculation formula from the Chinese Code for Design of Composite Structures was used to predict the maximum lateral load capacity. The comparison shows good agreement between the test and predicted results.

• Asian Journal for Public Opinion Research
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• 제3권4호
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• pp.156-175
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• 2016

This paper considers whether random sampling always produces more accurate survey results in the case of South Korea. We compare information from the 2010 census to the demographic variables of three public opinion surveys from South Korea: Gallup Korea's Omnibus Survey (Survey A) is conducted every two months by Gallup Korea; the annual Social Survey (Survey B) is conducted by Statistics Korea (KOSTAT); the Korean General Social Survey (KGSS or Survey C) is conducted annually by the Survey Research Center (SRC) at Sungkyunkwan University (SKKU). Survey A uses quota sampling after randomly selecting the neighborhood and initial addresses; Survey B uses random sampling, but allows replacements in some situations; Survey C uses simple random sampling. Data from more than one year was used for each survey. Our analysis suggests that Survey B is the most representative in most respects, and, in some respects, Survey A may be more representative than Survey C. Data from Survey C was the least stable in terms of representativeness by geographical area and age. Single-person households were underrepresented in both Surveys A and C, but the problem was more severe in Survey A. Four-person households and married persons were both over-represented in Survey A. Less educated people were under-represented in both Survey A and Survey C. There were differences in income level between Survey A and Survey C, but income data was not available for Survey B or the census, so it is difficult to ascertain which survey was more representative in this case.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2897-2901
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• 2012

Invasion is usually recognized as the main reason for the high recurrence and death rates of glioma and restricts the efficacy of surgery and other therapies. Therefore, we aimed to investigate the mechanism involved in promotion effects of mda-9/syntenin on human glioma cell migration. The wound healing method was used to test the migration ability of human glioma cells CHG-5 and CHG-hS, stably overexpressing mda-9/syntenin. Western blotting was performed to determine the expression and phosphorylation of focal adhesion kinase (FAK) and JNK in CHG-5 and CHG-hS cells. The migration ability of CHG-hS cells was significantly higher than that of CHG-5 cells in fibronectin (FN)-coated culture plates. Phosphorylation of FAK on tyrosine 397, 576, and 925 sites was increased with time elapsed in CHG-hS cells. However, phosphorylated FAK on the tyrosine 861 site was not changed. Phosphorylated Src, JNK and Akt levels in CHG-hS cells were also significantly upregulated. Phosphorylation of JNK and Akt were abolished by the specific inhibitors SP600125 and LY294002, respectively, and the migration ability of CHG-hS cells was decreased, indicating that the JNK and PI3K/Akt pathways play important roles in regulating mda-9/syntenin-induced human brain glioma migration. Our results indicate Mda-9/syntenin overexpression could activate FAK-JNK and FAK-Akt signaling and then enhance the migration capacity of human brain glioma cells.

• 한국정보통신학회논문지
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• 제21권12호
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• pp.2232-2240
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• 2017

국제해상기구는 국제해상인명안전협약을 통해 국제항해 선박에 대하여 AIS와 VHF를 의무화하고, 국내 또한 선박안전법과 선박설비기술기준을 통해 특정 선박에 의무화 하고 있다. 그러나 다양한 통신장비 및 복잡한 사용법으로 인해 오동작이 발생하고 실제 구조신호에 대한 응답지연으로 인하여 인명사고가 발생하는 경우가 많다. 그래서 최근 해상통신장치는 국제해상안전조난시스템 현대화의 일환으로 차세대 해상통신시스템 구축을 위해 여러 종류의 해상통신시스템을 연동 및 통합하려는 시도가 이루어지고 있다. 본 논문에서는 AIS와 VHF의 연동 및 통합을 통한 DSC 기능을 구현하는 기법에 대하여 기술하였다. AIS에서 선박의 정보를 추출하고 VHF의 DSC 기능에 활용할 수 있는 데이터의 연동 알고리즘을 통해 향 후 국내기술기준 및 표준화를 달성하기위한 근거를 제시하고자 한다.

• 한국강구조학회 논문집
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• 제14권3호
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• pp.463-470
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• 2002

TEC-BEAM System은 구조용 CT형강, PC 콘크리트 그리고 현장타설콘크리트 슬래브로 이루어진 합성보이다. 이 시스템은 단순보에 대한 휨 및 전단실험을 수행하였고 우수한 거동을 보였다. 그러나 현장적용을 위해서는 TEC-BEAM과 RC 기둥 강접합 상세개발이 필요하다. 이 접합부는 TEC-BEAM의 하부에 발생하는 힘을 기둥에 전달하기 위한 매캐니즘을 발생해야만 한다. 그래서 TEC-BEAM의 단면이 증대되어 TEC-BEAM 플랜지 하부로 철근이 배근되는 접합부를 개발하였다. 본 논문에서는 제안된 시스템의 구조적 성능 평가를 위해 2개의 실험체에 대하여 실험하였다. 실험변수는 철근의 정착길이 즉, 증대된 단면의 길이이며, 실험결과 제안된 시스템은 우수한 접합부를 구조 성능을 가지고 있음을 확인할 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권8호
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• pp.3793-3796
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• 2016

Background: Prostate cancer (PC) is one of the leading causes of death, especially in developed countries. The human development index (HDI) and its dimensions seem correlated with incidence and mortality rates of PC. This study aimed to assess the association of the specific components of HDI (life expectancy at birth, education, gross national income per 1000 capita, health, and living standards) with burden indicators of PC worldwide. Materials and Methods: Information of the incidence and mortality rates of PC was obtained from the GLOBOCAN cancer project in year 2012 and data about the HDI 2013 were obtained from the World Bank database. The correlation between incidence, mortality rates, and the HDI parameters were assessed using STATA software. Results: A significant inequality of PC incidence rates was observed according to concentration indexes=0.25 with 95% CI (0.22, 0.34) and a negative mortality concentration index of -0.04 with 95% CI (-0.09, 0.01) was observed. Conclusions: A positive significant correlation was detected between the incidence rates of PC and the HDI and its dimensions including life expectancy at birth, education, income, urbanization level and obesity. However, there was a negative significant correlation between the standardized mortality rates and the life expectancy, income and HDI.