• Animal cells and systems
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• v.5 no.2
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• pp.145-151
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• 2001

p62 is a phosphotyrosine-independent ligand of the SH2 domain of $p56^{Ick}$, a T-cell specific Src family tyrosine kinase. Recently p62 has been shown to interact with a number of proteins, such as $PKC\varsigma$ and ubiquitin, and implicated in important cellular functions such as cell proliferation. Since the two p62 interacting proteins, $p56^{Ick}$ and $PKC\varsigma$, have been reported to play roles in cell death, 1 have addressed the potential role of p62 during apoptosis in Jurkat cells in this study. Herein 1 show that p62 was specifically cleaved into two peptides by a caspase-3-like activity during Fas-receptor mediated apoptosis in Jurkat cells. This cleavage generated two fragments with molecular weights of about 35 kDa that differed in subcellular localizations. The N-terminal cleaved fragment was present in the detergent-insoluble fraction whereas the C-terminal fragment was found in the detergent-soluble fraction. In addition, the C-terminal fragment appeared to be subjected to further degradation as apoptosis prolonged. Moreover, overexpression of p62 in Jurkat cells attenuated the Fas receptor mediated apoptosis, suggesting that p62 is involved in apoptotic signal transduction pathway in lymphocytes.

• Animal cells and systems
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• v.14 no.3
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• pp.155-160
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• 2010

Extracellular signal-regulated kinases 1/2 (ERK1/2) play important roles in a variety of biological processes including cell growth and differentiation. We have previously reported that GAR-3 activates ERK1/2 via phospholipase C and protein kinase C, presumably through pertussis toxin (PTX)-insensitive Gq proteins, in Chinese hamster ovary (CHO) cells. Here we provide evidence that GAR-3 also activates ERK1/2 through PTX-sensitive G proteins, phosphatidylinositol 3-kinase (PI 3-kinase), and Src family kinases in CHO cells. We further show that in human embryonic kidney (HEK293) cells, epidermal growth factor receptor and Ras are required for efficient ERK1/2 activation by GAR-3. Taken together, our data indicate that GAR-3 evokes ERK1/2 activation through multiple signaling pathways in cultured mammalian cells.

• Steel and Composite Structures
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• v.39 no.3
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• pp.337-352
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• 2021

The study presented experimental and numerical investigation on the seismic performance of steel reinforced concrete (SRC) L-shaped column- reinforced concrete (RC) beam joints. Various parameters described as steel configuration form, axial compressive ratio, loading angle, and the existence of slab were examined through 4 planar joints and 7 spatial joints. The characteristics of the load-displacement response included the bearing capacity, ductility, story drift ratio, energy-dissipating capacity, and stiffness degradation were analyzed. The results showed that shear failure and flexural failure in the beam tip were observed for planar joints and spatial joint, respectively. And RC joint with slab failed with the plastic hinge in the slab and bottom of the beam. The results indicated that hysteretic curves of spatial joints with solid-web steel were plumper than those with hollow-web specimens. The capacity of planar joints was higher than that of space joints, while the opposite was true for energy-dissipation capacity and ductility. The high compression ratio contributed to the increase in capacity and initial stiffness of the joint. The elastic and elastic-plastic story deformation capacity of L-shaped column frame joints satisfied the code requirement. A design formula of joint shear resistance based on the superposition theory and equilibrium plasticity truss model was proposed for engineering application.

• Steel and Composite Structures
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• v.32 no.2
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• pp.265-279
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• 2019

The self-centering capacity and energy dissipation performance have been recognized critically for increasing the seismic performance of structures. This paper presents an innovative steel moment frame with self-centering steel reinforced concrete (SRC) wall panel incorporating replaceable energy dissipation devices (SF-SCWD). The self-centering mechanism and energy dissipation mechanism of the structure were validated by cyclic tests. The earthquake resilience of wall panel has the ability to limit structural damage and residual drift, while the energy dissipation devices located at wall toes are used to dissipate energy and reduce the seismic response. The oriented post-tensioned strands provide additional overturning force resistance and help to reduce residual drift. The main parameters were studied by numerical analysis to understand the complex structural behavior of this new system, such as initial stress of post-tensioning strands, yield strength of damper plates and height-width ratio of the wall panel. The static push-over analysis was conducted to investigate the failure process of the SF-SCWD. Moreover, nonlinear time history analysis of the 6-story frame was carried out, which confirmed the availability of the proposed structures in permanent drift mitigation.

• Smart Structures and Systems
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• v.26 no.6
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• pp.781-796
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• 2020

Prediction of total sediment load is essential in an extensive range of problems such as the design of the dead volume of dams, design of stable channels, sediment transport in the rivers, calculation of bridge piers degradation, prediction of sand and gravel mining effects on river-bed equilibrium, determination of the environmental impacts and dredging necessities. This paper is aimed to investigate and predict the total sediment load of the Wadi Arbaat in Eastern Sudan. The study was estimated the sediment load by separate total sediment load into bedload and Suspended Load (SL), independently. Although the sediment records are not sufficient to construct the discharge-sediment yield relationship and Sediment Rating Curve (SRC), the total sediment loads were predicted based on the discharge and Suspended Sediment Concentration (SSC). The turbidity data NTU in water quality has been used for prediction of the SSC in the estimation of suspended Sediment Yield (SY) transport of Wadi Arbaat. The sediment curves can be used for the estimation of the suspended SYs from the watershed area. The amount of information available for Khor Arbaat case study on sediment is poor data. However, the total sediment load is essential for the optimal control of the sediment transport on Khor Arbaat sediment and the protection of the dams on the upper gate area. The results show that the proposed model is found to be considered adequate to predict the total sediment load.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.17 no.4
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• pp.1182-1199
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• 2023

Fractional repetition (FR) codes can achieve exact uncoded repair for multiple failed nodes, with lower computational complexity and bandwidth overhead, and effectively improve repair performance in distributed storage systems (DSS). The actual distributed storage system is dynamic, that is, the parameters such as node storage overhead and number of storage nodes will change randomly and dynamically. Considering that traditional FR codes cannot be flexibly applied to dynamic distributed storage systems, a new construction scheme of adaptive-and-resolvable FR codes based on hypergraph coloring is proposed in this paper. Specifically, the linear uniform regular hypergraph can be constructed based on the heuristic algorithm of hypergraph coloring proposed in this paper. Then edges and vertices in hypergraph correspond to nodes and coded packets of FR codes respectively, further, FR codes is constructed. According to hypergraph coloring, the FR codes can achieve rapid repair for multiple failed nodes. Further, FR codes based on hypergraph coloring can be generalized to heterogeneous distributed storage systems. Compared with Reed-Solomon (RS) codes, simple regenerating codes (SRC) and locally repairable codes (LRC), adaptive-and-resolvable FR codes have significant advantages over repair locality, repair bandwidth overhead, computational complexity and time overhead during repairing failed nodes.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2020.10a
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• pp.221-221
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• 2020

Curcumin, a hydrophobic polyphenol derived from turmeric, has been used a food additive and as a herbal medicine for the treatment of various diseases. In the present study, we found the functional role of a nanosphere loaded with curcumin (CN) in the promotion of the motility of human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) during the wound closure. We found that the efficacy of hUCB-MSCs migration induced by CN was 1000-fold higher than that of curcumin powder. CN significantly increased the motility of hUCB-MSCs by activating c-Src, which is responsible for the phosphorylation of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK). CN induced the expression levels of α-actinin-1, profilin-1 and filamentous-actin, as regulated by the phosphorylation of nuclear factor-kappa B during its promotion of cell migration. In a mouse skin excisional wound model, we found that transplantation of UCB-MSCs pre-treated with CN enhances wound closure, granulation, and re-epithelialization at mouse skin wound sites. These results indicate that CN is a functional agent that promotes the mobilization of UCB-MSCs for cutaneous wound repair.

• Structural Engineering and Mechanics
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• v.88 no.1
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• pp.25-42
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• 2023

This paper presents a finite element (FE) simulation of eccentrically loaded lightweight aggregate concrete-encased steel (LACES) columns with H-shaped steel sections, analytical equations are also established to estimate the columns' axial and bending moment interaction capacities. The validity of the proposed models is checked by comparing the results with experimental data. Good agreements between the test and proposed models' results are found with acceptable agreements. Moreover, design parameters, including the lightweight aggregate concrete (LWAC) strength, eccentricity, column slenderness ratio, and confinement, are studied using the FE analysis, and their efficiency factors are discussed. The results show that the ultimate axial capacity of the LACES composite columns subjected to eccentric loading is negatively affected by the increase in the columns' height, but it is positively affected by the increase of the confinement. Increasing the eccentricity and columns' height reduced the columns'stiffness. In addition, the ultimate capacity of the LACES column is significantly influenced by the LWAC strength and eccentricity, where the ultimate capacity of the LACES column is significantly increased by increasing LWAC strength, and it is remarkably decreased by increasing the eccentricity. When the eccentricity changed from zero to 70 mm, the ultimate axial capacity and stiffness decreased by 67.97% and 63.56%, respectively.

• Journal of Navigation and Port Research
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• v.48 no.2
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• pp.125-136
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• 2024

This research presents an innovative integrated ethanol solid oxide fuel cell (SOFC) system designed for applications in marine vessels. The system incorporates an exhaust gas heat recovery mechanism. The high-temperature exhaust gas produced by the SOFC is efficiently recovered through a sequential process involving a gas turbine (GT), a regenerative system, steam Rankine cycles, and a waste heat boiler (WHB). A comprehensive thermodynamic analysis of this integrated SOFC-GT-SRC-WHB system was performed. A simulation of this proposed system was conducted using Aspen Hysys V12.1, and a genetic algorithm was employed to optimize the system parameters. Thermodynamic equations based on the first and second laws of thermodynamics were utilized to assess the system's performance. Additionally, the exergy destruction within the crucial system components was examined. The system is projected to achieve an energy efficiency of 58.44% and an exergy efficiency of 29.43%. Notably, the integrated high-temperature exhaust gas recovery systems contribute significantly, generating 1129.1 kW, which accounts for 22.9% of the total power generated. Furthermore, the waste heat boiler was designed to produce 900.8 kg/h of superheated vapor at 170 ℃ and 405 kP a, serving various onboard ship purposes, such as heating fuel oil and accommodations for seafarers and equipment.

• International Journal of Industrial Entomology and Biomaterials
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• v.48 no.1
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• pp.13-18
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• 2024

The rice field grasshopper, Oxya chinensis sinuosa (OC), has traditionally been utilized in Korea for various purposes; however, its potential benefits in the context of osteoporosis remain unclear. The results revealed that OC ethanol extract (OCE) significantly inhibited the formation and activity of tartrate-resistant acid phosphatase (TRAP)-positive cells in receptor activator of nuclear factor-κB ligand (RANKL)-stimulated RAW264.7 cells. Furthermore, OCE, at concentrations ranging from 100 to 400 ㎍/mL, demonstrated a dose-dependent reduction in the protein expression of osteoclast-specific markers, including nuclear factor of activated T cell cytoplasmic 1, c-Src, and TRAP, when compared to RANKL stimulation alone. Additionally, OCE significantly inhibited RANKL-induced activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) but not the activation of extracellular signal-regulated kinase. Collectively, these results indicate that OCE suppresses osteoclastogenesis by attenuating the phosphorylation of p38 MAPK and JNK. Consequently, these findings suggest that OCE holds promise for the prevention of osteoporosis.