• The Plant Pathology Journal
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• 제20권3호
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• pp.212-219
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• 2004

Three isolates of Cucumber mosaic virus (CMV) from lily plants showing mosaic and distortion symptoms were detected by reverse-transcriptase polymerase chain reaction (RT-PCR) using primers specific to Cucumovirus genus namely, LK-CMV, LK4-CMV, and LKS-CMV. Restriction enzymes patterns of the RT-PCR products revealed that the lily isolates belonged to subgroup IA of CMV. In terms of biological properties, the lily isolates have highly similar but distinct pathogenicity as reported in other lily strains and ordinary strains of CMV. To characterize the molecular properties, cDNAs containing coat protein (CP) gene and 3' non-coding region (NCR) of RNA3 for the isolates were cloned and their nucleotide sequences were determined. The CP similarity (218 amino acids) was highly homologous (>97％) with that of subgroup I CMV strains. However, an additional 20-nulcleotide long segment was only present in 3' NCR of lily isolates, which form an additional stem-loop RNA structure. By using chimeric construct exchange cDNA containing 3'NCR of LK-CMV into the full-length cDNA clone of RNA3 of Fny-CMV, this additional segment may prove to be significant in the identification and fitness of the virus in lily plants. The pathology of zucchini squash infected by F1F2L3-CMV, a pseudorecombinant virus was showed to change drastically the severe mosaic and stunting symptom into a mild chlorotic spot on systemic leave, compared with Fny-CMV. To delimit the sequence of RNA3 affected the pathology, various RNA3 chimeras were constructed between two strains of CMV. The symptom determinants of F1F2L3-CMV were mapped to the positions amino acid 234, 239, and 250 in 3a movement protein (MP). RNA3 chimeras changed the sequences encoding three amino acids were resulted in alteration of systemic symptom.

• 디지털융복합연구
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• 제15권11호
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• pp.497-511
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• 2017

연구의 목적은 스쿼시 심판판정에 대한 선수들의 인식과 심판판정에 대한 정서 반응을 알아보는데 있다. 이를 위해, 2016년 국가대표선수 남 녀 각 3명 총 6명을 대상으로 개별심층면담을 진행하여, 이들이 인식하고 있는 심판판정과 그에 따른 정서반응을 규명하였다. 연구결과 첫째, 심판판정에 대한 인식 차이는 심판마다 주관적 관점을 가지고 있고 보는 위치와 시각의 차이, 순간적인 판단능력, 경력과 경험에 따라 차이가 나는 것으로 나타났다. 둘째, 심판판정이 경기의 승패에 미치는 영향은 낮은 것으로 나타났다. 셋째, 리드하고 리드 당하는 경기 상황에서 심판 판정 영향정도와 그에 따른 정서적 대처방안 모두 "영향이 없다"는 의견이 많은 것으로 나타났다. 이러한 결과는 선수들이 인식하는 심판판정이 경기상황과 심판의 경험 등에 따라서 다르게 인식한다는 것을 의미하며, 심판에 따라 판정에 대한 인식이 다르지만, 실제 경기결과에는 영향을 미치지 않는다는 것을 의미한다. 결론적으로 심판판정이 상황에 따라 선수들의 정서반응에 긍정적 및 부정적 영향을 미칠 수 있지만, 경기력을 결정하는 중요 요소는 선수 개인의 여유 있는 경기 운영과 기술의 정확성이라는 사실을 시사한다.

• 한국식품영양학회지
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• 제33권1호
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• pp.91-97
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• 2020

The purpose of this study was to provide the basic data of butternut squash (Cucurbita moschata) according to the drying methods. The proximate composition and chromaticity of freeze-dried and hot air-dried Cucurbita moschata powders were evaluated, and the antioxidant effects of these ethanolic extracts were analyzed by DPPH, ABTS, FRAP, reducing power activity, total phenolic, and flavonoid content. As a result, Cucurbita moschata was revealed to have high carbohydrate and crude protein contents, while crude ash and crude fat contents were relatively low. Also, it was confirmed that the hot air-dried Cucurbita moschata had lower L, a, and b values than the freeze-dried Cucurbita moschata. Additionally, as a result of analyzing the antioxidant activity of Cucurbita moschata ethanolic extracts, hot air-dried Cucurbita moschata ethanolic extract showed significantly superior antioxidant activity than freeze-dried Cucurbita moschata ethanolic extract. Also, hot air-dried Cucurbita moschata ethanolic extract was revealed to have a higher polyphenol content, but slightly lower flavonoid content than freeze-dried Cucurbita moschata ethanolic extract. Based on the data from this study, further experiments on Cucurbita moschata material are necessary.

• Journal of Photoscience
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• 제7권2호
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• pp.39-44
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• 2000

To examine the chilling tolerance lipids, we compared the chilling susceptibility of photosystem II of wild type tobacco plants with that of transgenic tobacco plants, in which the sensitivity to chilling had been enhanced by genetic modification of fatty acid unsaturation of chloroplast membrane lipids. The transgenic tobacco plants were found to contain reduced levels of unsaturated membrane fatty acids by being tansformed with cDNA for glycerol-3-phosphate acyltransferase from squash. For the purpose of studying on the functional integrity of photosystem II during low-temperature photoinhibition, the photochemical efficiency was measured as the ration of the maximun fluorescence of chlorophyll (Fv/Fm) of photosystem II. In parallel with an investigation on the transgenic plants, susceptibility of chilling-resistant species, such as spinah and pea, and of chilling-sensitive ones, such as squash and sweet potato, to low-temperature photoinhibition was also compared in terms of room temperature-induced chlorophyll fluorescence from photosystem II. When leaf disks from the two genotypes of tobacco plants were exposed to light at 5$^{\circ}C$, the transgenic plants showed more rapid decline in photochemical activity of photosysytme II than wild-type plants. When they were pretreated with lincomycin, an inhibitor of chloroplast-encoded protein synthesis, the extent of photoinhibition was even more accelerated. More impottantly, they showed a comparable extent of photoinhibition in the presence of lincomycin, making a clear contrast to the discrepancy observed in the discrepancy observed in the absence of lincomycin. Restoration of Fv/Fm during recovery from low-temperature photoinhibition occurred more slowly in the transgenic tobacco plants than the wild-type. These findings are discussed in relation to fatty acid unsaturation of membrane phosphatidylglycerol. It appears that the ability of plants to rapidly regenerate the active photosystem II complex from might explain, in part, why chilling-resistant plants can toleratlow-temperature photoinhibition.

• 한국컴퓨터그래픽스학회논문지
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• 제18권2호
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• pp.55-62
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• 2012

본 논문에서는 GPU의 정점 셰이더에서 각 정점별 질량-스프링 모의 실험을 수행하여, 매우 빠른 속도로 피부의 2차 변형을 표현하는 새로운 방법을 제안한다. 3차원 캐릭터의 각 피부 정점에 대해, 길이가 0인 스프링으로 가상 정점을 연결하고, 캐릭터 동작에 따라서 피부 정점의 위치 및 속도가 변화하면, 질량-스프링 모의 실험을 통해 렌더링 될 가상 정점의 위치를 GPU의 정점 셰이더를 사용하여 병렬적으로 계산한다. 본 논문에서 제시하는 방법을 통해 피부의 재질 특성을 반영하는 2차 변형을 매우 빠르게 표현할 수 있으며, 각 피부 정점에 제안한 방법을 동적으로 적용하면 전통적인 2차원 애니메이션에서 관찰되는 수축-팽창(squash-and-stretch), 추종(follow-through)과 같은 효과도 매우 빠른 연산시간에 효과적으로 표현할 수 있다. 따라서 본 논문의 결과는 게임과 같은 실시간 응용분야에서 가상 캐릭터의 피부 변형을 표현하는데 효과적으로 사용될 수 있다.

• 한국운동역학회지
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• 제17권1호
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• pp.29-39
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• 2007

This study were obtained elapsed time phase-by-phases, displacement, user angle, velocity and angular velocity to analyse kinematically contributing factors at impact of forehand drive motion, on targeting three male players. The results of the study were presented as follows; In the forehand drive swing, the elapsed time by phases was a total of .52 seconds: .30 seconds from backswing to impact and .22 seconds from impact to follow-through, Considering the mean change in locations of COM of each(part$\rightarrow$body segment) at impact, racket head, left shoulder, right wrist and left hip, the left-right directions(X-axis) were showm to be each $.61{\pm}.03$, $1.19{\pm}.08$, $.66{\pm}.03$, $.94{\pm}.06$, and $.45{\pm}.03m$. The displacement differences of COM of each body segment were shown to be -.57, -.05, -.33, and .16m. For the vertical direction(Z-axis), the center of mass was lowest at impact and highest at E3. For the displacement of the right wrist on the left hip, the right wrist moved to .82m to the lower direction without change in the locations of the hip from E1 from E2. When the left hip moved .02m from E2 to E3, the right wrist moved .7m in the upper direction. In respect to the velocity of each body segment, the hip and the shoulder joint accelerated and then the wrist followed. Then the right wrists of all the subjects and their racket heads showed maximum speed, and an effective swing was observed. At the angle of each part, the angle of the right wrist was the smallest at the backswing and the largest at the moment of the impact. Then it increased gradually in the follow-through section. In respect of angular velocity for subject A, the hip moved and the largest change occurred. Immediately before the impact, the subject made a swing using his right wrist, his hip, and the shoulder joint, showing the maximum value, which was judged to be effective.

• Journal of Radiation Protection and Research
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• 제16권2호
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• pp.55-65
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• 1991

벼, 콩, 상치, 당근, 호박의 가식부위에 대하여 몇 가지 토양에 있어서 토양-작물체간 Mn-54, Co-60, Zn-65, Cs-137의 전이계수를 포트 재배에 의한 방사성 추적자 흡수실험을 통하여 조사하였다. 핵종간 전이계수는 거의 모든 경우 Zn-65>Mn-54>Cs-137>Co-60의 순이었다 중이 벼보다 전반적으로 한 자리 정도 높은 값을 보였고 채소류의 경우에는 대체로 상치에서 가장 높고 호박에서 가장 낮은 값을 보였다. 강산성 토양에서는 약산성 토양에서보다 전이계수가 철센 높았다. 본 조사결과에 입각하여 한국인의 섭식경로 피폭선량 평가에 이용하기 위한 각 핵종의 전이계수치가 작물별로 제안되었다.

• 한국운동역학회지
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• 제17권3호
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• pp.11-21
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• 2007

The purpose of this study was to examine the differences of kinematics and muscle activities depending on the changes of angle approaching balls during backhand drive in squash. The results are as follows. Stride time took the longest at AD2 and step lengths were the biggest at AD1 of left foot contact and right foot contact and AD2 of impact and follow-through. The center of gravity and the speed of racket head were the highest at AD3 and at AD2. Angle of shoulder joint were the biggest at AD1 of left foot contact, right foot contact and impact and AD3 of follow-through. Angle of elbow joint were the biggest at AD3 of left foot contact, right foot contact and follow-through and AD2 of impact. Angle of pelvis joint were the biggest at AD2 of left foot contact, AD1 of right foot contact and AD3 of impact and follow-through. Angle of knee joint were the biggest at AD2 of left foot contact, AD1 of right foot contact and AD3 of impact and follow-through. Angle of ankle joint were the biggest at AD1 of left foot contact and AD3 of right foot contact, impact and follow-through. According to the analysis results of triceps brachii, latissimus dorsi, brachioradialis muscle and flexor carpi ulnaris muscle activities were high at AD1 of all phases. Analysis results of vastus lateralis, vastus medialis, tibialis anterior and gastrocnemius medial muscle activities were high at AD2 of phase1 and phase3. Those of vastus lateralis, vastus medialis and tibialis anterior, gastrocnemius medial were high at AD3 of Phase 2 and AD1 of phase2.

• 한국식생활문화학회지
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• 제37권6호
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• pp.540-546
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• 2022

This study aimed to determine the changes in the vitamin B₅ content of raw and cooked vegetables. The nineteen vegetables were subjected to different cooking methods, viz. blanching, boiling, pan-broiling, and steaming. Vitamin B₅ was quantified by reversed-phase high-performance liquid chromatography (HPLC) using photodiode-array (PDA) detection (200 nm). The standard reference materials (SRM) were used to validate the accuracy of vitamin B₅ measurement method used in this study. The cooking yields ranged from 82.63 to 107.62% and decreased in most of the vegetables except bitter melon, curled mallow, and eggplant. The raw kabocha squash, Danhobak, had the highest vitamin B₅ content (0.671 mg/100 g) among the samples. All cooked vegetables showed lower vitamin B₅ content compared to the raw samples. The true retention ranged from 0% (crown daisy, blanching) to 84.49% (kabocha squash, steaming). These results indicate that vitamin B₅ is degraded after cooking. Pan-broiling and steaming are better cooking methods than the others for retaining vitamin B₅. The true retention of vitamin B₅ in the samples markedly depends on the cooking method and food matrix. These results can be used as important basic data for nutritional evaluation of meals.

• 한국식생활문화학회지
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• 제37권1호
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• pp.47-60
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• 2022

This study examines the effects of different cooking methods (stir-frying, steaming, superheated-steaming) on the contents and the true retention of moisture, water-soluble vitamins, and bioactive compounds of ten selected vegetables: broccoli, brussels sprout, cabbage, eggplant, green bean, onion, red cabbage, red onion, squash, and tomato. The total color difference (𝚫E) values were decreased after stir-frying the samples, except for eggplant, green bean, and tomato. The true retention of water-soluble vitamins (B₁, B₂, B₃, and C) was increased in most vegetables after superheated-steaming, as compared to steaming and stir-frying. Moreover, compared to the uncooked vegetables, a higher true retention of total polyphenol and flavonoid was obtained for most vegetables subsequent to superheated-steaming. Total anthocyanin content was detected only in eggplant, red cabbage, and red onion, and a smaller loss of anthocyanin was determined after subjecting red cabbage to superheated-steaming. Also, the free radical scavenging activities were higher in superheated-steaming vegetables, except in eggplant and squash. These results indicate that superheated-steaming induces a positive effect for retaining water-soluble vitamins and functional components of vegetables.