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Epidemiological study of bacterial diseases of cultured olive flounder, Paralichthys olivaceus from 2005 to 2006 in Korea (2005년부터 2006년 사이 우리나라 양식 넙치, Paralichthys olivaceus의 세균성 질병에 대한 역학조사)

  • Cho, Mi-Young;Kim, Myoung-Sug;Kwon , Mun-Gyeong;Jee, Bo-Young;Choi, Hye-Sung;Choi, Dong-Lim;Park, Gyeong-Hyun;Lee, Chang-Hoon;Kim, Jin-Do;Lee, Joo-Seok;Oh, Yun-Kyeong;Lee, Deok-Chan;Park, Shin-Hoo;Park, Myoung-Ae
    • Journal of fish pathology
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    • v.20 no.1
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    • pp.61-70
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    • 2007
  • The epidemiological study was performed to survey the prevalence of bacterial disease of cultured olive flounder, Paralichthys olivaceus from October, 2004 to August, 2006 in Korea. A total of 1,271 of fish samples were collected at random includes fish exhibiting clinical signs of the disease in question. The total 331 samples among 738 cases of infectious diseases were infected with 366 bacteria isolates including Vibrio spp. (42.1%), Streptococcus spp. (16.9%), Edwardsiella tarda (12.3%), Photobacterium damselae subsp. damselae (8.2%), Pseudomonas spp. (2.2%) or others (18.3%). Vibrio spp. and P. damselae subsp. damselae were continually isolated through all seasons but Streptococcus spp. and E. tarda were mainly isolated from May to November. The 206 cases were showed mixed infection with other bacteria (3.6%), parasites (31.4%) or virus (41.7%); Vibrio spp. (n=21), Streptococcus spp. (n=13), Trichodina (n=76), Scutica (n=31), VNNV (n=112), VHSV (n=46).

Distribution and Biodegradation of Crude oil-Degrading Bacteria in P'ohang Coastal Area (포항근해 원유분해세균의 분포 및 원유분해능)

  • 이창호;권기석;서현호;김희식;오희목;윤병대
    • Journal of Korea Soil Environment Society
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    • v.1 no.2
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    • pp.35-42
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    • 1996
  • Seawater samples were collected from P'ohang coastal area during April 1995 - January 1996. The distribution of total heterotrophic bacteria and crude oil-degrading bacteria (CDB) were studied. In addition, biodegradation of crude oil was investigated through mono and mixed culture. The heterotrophic bacterial distribution was in the range of 4.1 $\times$ $10^4$- 1.2 $\times$ $10^5$ CFU/$m\ell$, respectively. The percent of crude oil-degrading bacteria against total heterotrophic bacteria was 0.05-0.54% which was lower than other marine samples reported. Therefore it could be suggested that the distribution of crude oil-degrading bacteria in the seawater of P'ohang coastal area was highly affected by presence of petroleum hydrocarbon. Taxonomical characteristics of 26 isolates were investigated. The results of identification were showed 7 genera which were Acinetobacter spp., Bacillus spp., Citrobacter spp., Micrococcus spp., Moraxella spp., Rhodococcus spp., and Serratia spp. Appearance of Enterobacteriaceae indicated that the seawater was polluted with wastewater. Also genus of Bacillus had predominant in CDB on P'ohang coastal area. In flask culture, biodegradation of crude oil was enhanced by addition of mixed culture of CDB.

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A Survey on the population of Toxigenic Fungi in Crude Drugs in Seoul (시판 유통중인 한약재에서의 위해성 진균 분포에 관한 연구)

  • Lee Young-Ki;Park Ok-Soon;Oh Young-Hee;Kim Mu-Sang;Kim Young-Soo
    • Journal of environmental and Sanitary engineering
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    • v.20 no.2 s.56
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    • pp.29-32
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    • 2005
  • We just carried out this study in order to obtain an appropriate information of the population of toxigenic fungi in crude drugs in seoul. Results of fungal examination on twenty-three kinds crude drugs were described in this report. In 21 crude drugs, colonies of fungi were possible to identify into 7 genera. Predominant genera of fungi in crude drugs were Aspergillus spp. $(14,\;46.6\%)$, Scopulariospsis spp. $(7,\;23.3\%)$, Penicillium spp., Rhizopus spp., Fusarium spp $(2,\;6.6\%,\;respectively)$ and Phoma sp., Chaetomium sp. $(1,\; 3.3\%,\;respectively)$. Mycotoxin producing fungi like Aspergillus spp., Penicillum spp., and Fusarium spp. were 18 colonies$(60\%)$, totally.

Aphelenchus avenae and Antagonistic Fungi as Biological Control Agents of Pythium spp.

  • Jun, Ok-Kyoung;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.20 no.4
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    • pp.271-276
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    • 2004
  • To examine the control effect of damping-off on radish caused by Pythium spp., researchers used the isolates of a fungivorous nematode, Aphelenchus avenae, and antagonistic fungi, Trichoderma spp. These were used as biocontrol agents, either alone, or in combination. Growth rates of the A. avenae isolates and fungal damages by the nematodes varied depending on Trichoderma spp., which contained lower T. koningii and T. virens cultures than other Trichoderma cultures. Phythium spp. were damaged by all five Aphelenchus isolates, but the multiplication rate of nematode isolate Aa-3 was very poor. Antibiotic activity of T. virens and T. harzianum to Pythium spp. was stronger than that of T. koningii. Control efficacy against damping-off of radish was most enhanced under the treatment using the nematode-T. harzianum combination. On the contrary, the combinations of the nematodes and T. virens or T. koningii mostly did not increase or decreased their control effect vis-$\`{a}$-vis that of the nematodes or antagonistic fungi being used alone. The results suggest that the fungivorous nematodes may play a leading role in the disease control, and that the activity of the fungivorous nematodes may be activated by T. harzianum, but inhibited by T. koningii and T.virens.

Identification of Filamentous Molds Isolated from Korean Traditional Nuruk and their Amylolytic Activties (한국전통누룩에 존재하는 사상균의 분리 동정 및 Amylolytic 효소 활성)

  • 박정웅;이계호;이찬용
    • Microbiology and Biotechnology Letters
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    • v.23 no.6
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    • pp.737-746
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    • 1995
  • Through the study of identification of filamentous molds isolated from 12 traditional Nuruk, collected from several regions in Korea, 78 strains of Absidia spp., including Absidia corymbifera, Absidia ramosa, and Absidia sp. of which specific names were not identified, 19 strains of Rhizopus spp. including Rhizopus cohnii, Rhizopus arrhizus, and Rhizopus oryzae, 8 strains of Circinella spp., 1 strain of Actinomucor sp., 49 strains of Aspergillus spp., including Aspergillus oryzae, Aspergillus flavus, Aspergillus flavus var. columnaris, Aspergillus wentii, Aspergillus candidus, Aspergillus fumigatus, Aspergillus niger, and Aspergillus foetidus, 2 strains of Cladospoyium spp. and 2 strains Botryotrichum spp., etc. total 159 kinds of filamentous molds were isolated and identified. There were many differences in numbers and distributions of filamentous molds from each Nuruk according to their collected region. Absidia spp. were most frequently isolated from every Nuruk sample. Aspergillus oryzae, Aspergillus candidus, and Rhizopus cohnii showed even distribution. Penicillium sp. and Mucor sp. were not detected. Actinomucor sp., Aspergillus foetidus, Botryotrichum sp., and Cladosporium spp., which have not been reported by far, were found. Amylase activities, pH stability of amylase, and acid productivity of isolated strains were compared.

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Oreochromis spp. and Clarias Lazera as a Source of Transmitting Encysted Metacercariae to Man

  • El-Gohary, A.H.;Samaha, I.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.4
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    • pp.439-443
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    • 1997
  • A total of 135 freshwater fish samples (Oreochromis spp. <85> and Clarias lazera <50>) were collected from different localities (shops and fish markets) in Alexandria Province-Egypt and examined for the presence of parasitic larval stages. The obtained data found the overall mean of the infestation with encysted metacercariae was 71.1% in the examined fish. The rates of infestation with encysted metacercariae were 72.9% and 68.0% in the examined Oreochromis spp. and Clarias lazera, respectively. The highest prevalence of the encysted metacercariae was found in summer (81.8%) in case of Oreochromis spp. and in winter (92.3%) in case of Clarias lazera, all these seasonal differences are statistically significant. The rate of infestation of encysted metacercariae in the anterior third, middle third and posterior third of Oreochromis spp. was found to be 82.3%, 74.2% and 95.2%, respectively, while 88.2%, 88.2% and 91.2%, respectively, in Clarias lazera. Moreover, the rate of infestation of encysted metacercariae in eyes, gills, liver, kidney and branchial cavity of Oreochromis spp. was 91.9%, 70.9%, 20.9%, 46.8% and 32.2%, respectively. In Clarias lazera the infestation rate was found to be 47.1%, 70.6%, 8.8% and 38.2% in the gills, liver, spleen and kidney, respectively. The zoonotic and public health importance of the encysted metacercariae are causes severe visceral pain, abdominal discomfort, intermittent bloody diarrhoea and colic after consumption of inadequately cooked fish especially Tilapia spp. (Oreochromis spp.).

Feeding Habits of Boleophthalmus pectinirostris in the Intertidal Zone of Beolgyo, Korea (벌교 조간대에 서식하는 짱뚱어 Boleophthalmus pectinirostris의 식성)

  • Choi, Dae-Up;Kim, Jong-Yeon
    • Korean Journal of Ichthyology
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    • v.28 no.2
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    • pp.87-92
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    • 2016
  • The feeding habits of Boleophthalmus pectinirostris were studied using 198 specimens collected monthly from April 2014 to March 2015 in the intertidal zone of Beolgyo, Korea. The size of B. pectinirostris ranged from 6.5 to 18.7 cm in total length (TL, cm). B. pectinirostris was herbivore that consumes mainly centric and pennate diatoms. Its diets also included small quantities of Prorocentrum dinoflagellates. and nematods. B. pectinirostris predominantly consumed diatoms (mainly Pleurosigma spp., Navicula spp., Nitzschia spp., Gyrosigma spp.) in all fish size classes, and all months, and thus there were no significant diet changes in relation to size and month.

Studies on the Flavonoid Compositions of Elsholtzia spp. (향유속(Elsholtzia spp.) 식물자원의 플라보노이드 성분 분석에 관한 연구)

  • Um, Hye-Jin;Kim, Gun-Hee
    • The Korean Journal of Food And Nutrition
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    • v.20 no.2
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    • pp.103-107
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    • 2007
  • Elsholtzia spp., a herbaceous perennial plant grown worldwide, have been used as a folklore medicine. Due to the presence of flavonoid in their extracts, Elsholtzia spp. have various functional properties. The objective of this research was to compare the amount of flavonoids in Elsholtzia spp. extracts in order to improve the potential of by using their functional properties. Elsholtzia spp. were collected in various areas of Korea. These were divided into leaf, flower, stem, and root. Each sample was dried using various drying method and ground to a powder. The powdered sample prepared was extracted with 80% ethanol. Extracts were analysed for the content of total flavonoids and apigenin by HPLC. Higher content of flavonoids was observed in the extracts of leaves and flowers. In previous study, apigenin among flavonoids was detected from Elsholtzia spp. so, apigenin content was analysed by HPLC. According to the results, the extracts of leaf and flower had the higher apigenin content than other samples.

Variation in trophic pathways and food web characteristics revealed by stable isotopes in an intermittent stream system of the Inukami River, Japan

  • Shin, Hyun-Seon;Amahashi, Nozomi;Ao, Lan;Mitamura, Osamu
    • Animal cells and systems
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    • v.15 no.3
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    • pp.235-241
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    • 2011
  • To examine variation in trophic pathways and the characteristics of food webs from organic matters to aquatic insects, we used stable isotopes to study an intermittent stream system of the Inukami River, Japan. The aquatic insects, including Glossosoma spp., Chironominae spp., Stenelmis spp., Rhyacophilla nigrocephala, and Hexatoma spp., were characterized by different feeding strategies. The ${\delta}^{13}C$ values for these species indicated that Glossosoma spp. graze upon periphyton; Chironominae and Stenelmis spp. mainly feed on benthic particulate organic matter, and R. nigrocephala and Hexatoma spp., which were identified as predators, feed upon Glossosoma, Stenelmis, and/or Chironominae spp. This suggests that the trophic position of consumers at each station may be determined by the trophic position of basal food sources in situ. For trophic pathways, the ${\delta}^{13}C$ values for both organic matter and aquatic insects tended to gradually decrease, whilst the ${\delta}^{15}N$ values increased from the upper reach to the lower reaches, relative to the physicochemical and geographical conditions. These parameters indirectly influence the flow of energy from organic matter to consumers within food web in an intermittent stream system.

Effects of Environmental Factors on Aeromonas spp. Population in Naktong Estuary (낙동강 하구 생태계의 환경요인과 Aeromonas spp. 분포와의 관계)

  • 전도용;권오섭;하영칠
    • Korean Journal of Microbiology
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    • v.27 no.4
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    • pp.391-397
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    • 1989
  • Population of Aeromonas and environmental parameters were investigated at three sites from August 1986, to December, 1986 in Naktong Estuary. The variation range of Aeromonas was $4.3\times10^{2}-4.6\times 10^{4}$ MPN/100ml. The result of ANOVA indicates significant differences among the populations of Aeromonas in each site. The highest population of Aeromonas occurred at site 2, and the lowest at site 3-B. To scrutinize the effects of environmental parameters on the distribution of Aeromonas spp, principal component analysis and multiple stepwise regression were used. The results showed that distribution of Aeromonas spp. was mainly influenced by outflow of freshwater and inflow of inorganic nutrients and correlated with heterotrophic bacteria, available nitrogen, fecal coliform bacteria, and temperature.

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