• Title/Summary/Keyword: SPOT

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Studies on Glycolipids in Bacteria -Part II. On the Structure of Glycolipid of Selenomonas ruminantium- (세균(細菌)의 당지질(糖脂質)에 관(關)한 연구(硏究) -제2보(第二報) Selenomonas ruminantium의 당지질(糖脂質)의 구조(構造)-)

  • Kim, Kyo-Chang
    • Applied Biological Chemistry
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    • v.17 no.2
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    • pp.125-137
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    • 1974
  • The chemical structure of glycolipid of Selenomonas ruminantium cell wall was to be elucidated. The bacterial cells were treated in hot TCA and the glycolipid fractions were extracted by the solvent $CHCl_3\;:\;CH_3OH$ (1 : 3). The extracted glycolipids fraction was further separated by acetone extraction. The acetone soluble fraction was named as the spot A-compound. The acetone insoluble but ether soluble fraction was named as the spot B-compound. These two compounds were examined for elucidation of their chemical structure. The results were as follows: 1. The IR spectral analysis showed that O-acyl and N-acyl fatty acids were linked to glucosamine moiety in the spot A-compound. However in the spot B-compound in addition to O and N-acyl acids phosphorus was shown to be attached to glucosamine. 2. It was recognized by gas liquid chromatography that spot A compound contained beta-OH $C_{13:0}$ fatty acid in predominance in addition to the fatty acid with beta-OH $C_{9:0}$, whereas the spot B compound was composed of the predominant fatty acid of beta-OH $C_{13:0}$ with small amount of beta-OH $C_{9:0}$. 3. According to the paper chromatographic analysis of hydrazinolysis products of the spot A compound, a compound of a similar Rf value as the chitobiose was recognized, which indicated a structure of two molecules glucosamine condensed. The low Rf value of the hydrazinolysis product of the spot B-compound confirmed the presence of phosphorus attached to glucosamine. 4. The appearance of arabinose resulting from. ninhydrin decomposition of the acid hydrolyzate of the spot A compound indicated that the amino group is attached to $C_2$ of glucosamine. 5. The amount of glucosamine in the N-acetylated spot A compound decreased in half of the original content by the treatment. with $NaBH_4$, indicating that there are two molecules of glucosamines in the spot A compound. The presence of 1, 6-linkage between two molecules of glucosamine was suggested by the Morgan-Elson reaction and confirmed by the periodate decomposition test. 6. By the action of ${\beta}-N-acetyl$ glucosaminidase the N-acetylated spot A compound was completely decomposed into N-acetyl glucosamine, whereas the spot B compound was not. This indicated the spot A compound has a beta-linkage. 7. When phosphodiesterase or phosphomonoesterase acted on $^{32}P-labeled$ spot B compound, $^{32}P$ was not released by phosphodiesterase, but completely released by phosphomonoesterase. This indicated that one phosphorus is linked to glucosamine moiety. 8. The spot A compound is assumed to have the following chemical structure: That is glucosaminyl, ${\beta}-1$, 6-glucosamine to which O-acyl and N-acyl fatty acids are linked, of which the predominant fatty acid is beta-OH $C_{13:0}$ fatty acid in addition to beta-OH $C_{9:0}$ fatty acid 9. The spot B compound is likely to have the linkage of $glucosaminyl-{\beta}-1$, 6-glucosamine to which phosphorus is linked in monoester linkage. Furthermore both O-acyl and N-acyl fatty acids contained beta-OH $C_{13:0}$ fatty acid predominantly in addition to beta-OH $C_{9:0}$ fatty acid.

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Statistical Investigation of Fatigue Life Prediction of the Spot Welded Lap Joint(I) : Application of Weibull Probability Distribution Function (정용접이음재의 피로수명 예측에 관한 확률적 검토(I) : Weibull 확률 분포함수 적용)

  • 손일선;백동호
    • Transactions of the Korean Society of Automotive Engineers
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    • v.7 no.6
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    • pp.214-221
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    • 1999
  • Spot welding is very important and useful technology in fabriaction of the thin sheet structure such as the automobile, train and air craft, Because fatigue strength of the spot welding point is however considerably lower than base metal due to stress concentration at the nugget edge, reasonable fatigue strength evaluation of spot welded lap joint is very important to estimate the reliability and durability of th spot welded structure and to establish a criterion of ling life fatigue design. For reasonalbe fatigue strength evaluation, it is necessary to estimate the fatigue strength of spot welded lap joints, systematically. So far, many investigators have numerically and experimentally studied on the systematic fatigue strength estimation for various spot welded lap joints, and the methods suggested has been considerably accumulated. By the way, for applying them in practical fatigue design of the thin sheet structure fabricated by spot welding ,it is also necessary to verify their efficiency and reliability on the predicted results, Therefore, in this study, a statistical fatigue strength estimation method for spot welded lap joints was developed by using the Weibull probability distribution function. From the result, it was found that fatigue strength and fatigue life of the spot welded lap joints having various dimension were able to be statically predicted . And also, a reliable criterion for long life fatigue design of the spot welded lap joint could be established.

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A Study on the Hot Spot Temperature in 154kV Power Transformers

  • Kweon, Dong-Jin;Koo, Kyo-Sun;Woo, Jung-Wook;Kwak, Joo-Sik
    • Journal of Electrical Engineering and Technology
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    • v.7 no.3
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    • pp.312-319
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    • 2012
  • The life of a power transformer is dependent on the life of the cellulose paper, which influenced by the hot spot temperature. Thus, the determination of the cellulose paper's life requires identifying the hot spot temperature of the transformer. Currently, however, the power transformer uses a heat run test is used in the factory test to measure top liquid temperature rise and average winding temperature rise, which is specified in its specification. The hot spot temperature is calculated by the winding resistance detected during the heat run test. This paper measures the hot spot temperature in the single-phase, 154kV, 15/20MVA power transformer by the optical fiber sensors and compares the value with the hot spot temperature calculated by the conventional heat run test in the factory test. To measure the hot spot temperature, ten optical fiber sensors were installed on both the high and low voltage winding; and the temperature distribution during the heat run test, three thermocouples were installed. The hot spot temperature shown in the heat run test was $92.6^{\circ}C$ on the low voltage winding. However, the hot spot temperature as measured by the optical fiber sensor appeared between turn 2 and turn 3 on the upper side of the low voltage winding, recording $105.9^{\circ}C$. The hot spot temperature of the low voltage winding as measured by the optical fiber sensor was $13.3^{\circ}C$ higher than the hot spot temperature calculated by the heat run test. Therefore, the hot spot factor (H) in IEC 60076-2 appeared to be 2.0.

An Experimental Investigation of Limit Loads and Fatigue Properties of Spot Welded Specimens (점용접시편의 극한하중과 피로특성에 관한 실험적 고찰)

  • Lee, Hyeong-Il;Kim, Nam-Ho;Lee, Tae-Su
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.24 no.1 s.173
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    • pp.38-51
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    • 2000
  • The study on the mechanical behavior of a spot-welded specimen is largely divided into the quasi-static overload failure analysis and the fatigue failure prediction. The main issue in an overload analysis is to examine the critical loads, thereby providing a generalized overload failure criterion. As the welding spot forms a singular geometry of an external crack type, fatigue failure of spot-welded specimens can be evaluated by means of a fracture parameter. In this study, we first present the limit loads of 4 representative types of single spot-welded specimens in terms of the base metal yield strength and specimen geometries. Recasting the load vs. fatigue life relationships experimentally, obtained here, we then predict the fatigue life of spot-weld specimens with a single parameter denoted the equivalent stress intensity factor. This crack driving parameter is demonstrated to successfully describe the effects of specimen geometry and loading type in a comprehensive manner. The suggested fatigue life formula for a single spot weld can play a key, role in the design and assessment of spot-welded panel structures, in that the fatigue strength of multi-spots is eventually determined by the fatigue strength of each single spot.

The comparision of fatigue behavior of $CO_2$ plug weld and resistance spot weld (저항점용접과 $CO_2$ 플러그용접의 피로거동 연구)

  • Jeong, Won-Uk;Jeong, Yeon-Su;Kang, Seong-Su
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.22 no.3
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    • pp.554-561
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    • 1998
  • Vehicle body structures are formed by thousands of spot welds and fatigue failure of vehicle structures occur near the spot welds after driving a long way at a durability test road. It is necessary to know accurately the reason of the fatigue failure of the spot weld in the developing stage in order to reinforce it. Many investigations have been done regarding the strength of spot welded joints, contributing to understand its fatigue strength. In developing process, a fatigue failed spot welded area can be repaired by $CO_2$ welding or another method to continue the test. To know the effect of reinforcing these welds, several methods of welding were analyzed and compared to spot welding. With the results of this test, the appropriate repair method can be used instead of spot welding during the development of new car and best design guide can be given for the strength. In this study, fatigue and static tensile tests are made and microstructure is investigated for the purpose of estimating the strength of welded joints by using spot welded and $CO_2$ plug welded specimens. The tested specimens are of two types : Tensile-shear type(TS) and L-tension type(LT).

Fracture Mechanical Fatigue Strength Evaluation of IB-Type Spot Welded Lap Joint under Tension-Shear Load (인장-전단하중을 받는 IB형 일점 Spot 용접이음재의 파괴역학적 피로강도 평가)

  • 손일선;정원석;이휘광;배동호
    • Transactions of the Korean Society of Automotive Engineers
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    • v.6 no.5
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    • pp.20-27
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    • 1998
  • According as the member of the automobile body structure have been thinned their thickness and have become high strength, each part of the body structure has been put more severe stress condition. And, because fatigue strength of the spot welded lap joint is influenced by its geometrical and mechanical factors, welding condition and etc., there needs a quantitative and systematic evaluation method for them. In this study, by considering nugget edge of the spot weld part of the IB-type spot welded lap joint under tension-shear load to the ligament crack. fatigue strength of various IB-type spot welded lap joints was estimated with the stress intensity factor(S.I.F.) KIII which is fracture mechanical parameter. We could find that fatigue strength evaluation of the IB-type spot welded lap joints by KIII is more effective than the maximum principal stress ($\sigma$1max) at edge of the spot weld obtained from FEM analysis.

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Comparison of SPOT-5 DEM Control with SRTM DEM (SRTM DEM을 이용한 SPOT-5 DEM의 조정 비교)

  • Lee, Hyo-Seong;Han, Dong-Yeob
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.29 no.2
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    • pp.193-199
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    • 2011
  • It is necessary to register precisely Digital Elevation Model (DEM) generated from SPOT -5 stereo images in order to monitor the topographic and environmental changes of important topographic features such as Mt. Baekdu. The SPOT-5 DEM is registered taking SRTM DEM as a reference DEM. If SPOT-5 DEM is transformed with single 3D rigid equation, it has many errors. Therefore, this study extracted uniformly ground control points over study area and estimated locally adapted transformation equation. The accuracy of proposed method was evaluated with comparison to scale-based and GCP-based transformation method.

The Correction of Systemetic Error of Three Dimensional Positioning using SPOT Imagery (SPOT 영상(映像)을 이용(利用)한 3차원(次元) 위치결정(位置決定)에 있어서 정오차(定誤差) 보정(補正)에 관한 연구(研究))

  • Yeu, Bock Mo;Jung, Young Dong;Lee, Hyun Jik
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.12 no.4_1
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    • pp.121-128
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    • 1992
  • This study aims to define the algorithm for self-calibration bundle adjustment with additional parameters, which is fit for the correction systematic errors in the SPOT satellite imagery, and to present a suitable term of additional parameters for the data form of SPOT satellite imagrery. As a result, an algorithm of self-calibration bundle adjustment for SPOT satellite imagery was settles, and the computer program was developed. Also, the suitable term of additional parameters to correct the systematic errors for each data form was defined through examination for determination effect of additional parameters and significance test. The algorithm of self-calibration bundle adjustment for SPOT satellite imagery according to this study could improve the accuracy of positioning.

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Fracture mechanical evaluation of fatigue strength of a single spot welded lap joint under tension-shear load (인장-전단하중을 받는 일점 Spot용접재의 파괴역학적 피로강도 평가)

  • 배동호
    • Journal of the korean Society of Automotive Engineers
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    • v.13 no.5
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    • pp.42-50
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    • 1991
  • According as the members and inner and outer plates of the automobile body structure have been thinned their thickness and have become high strength, each part of the body structure has been put more severe stress condition. Therefore, it has been increasingly required to improve the fatigue strength of the spot welded structures. As one of the improving methods for such problem, the author had previously proposed the method of alleviating stress concentration at nugget edge of the spot weld part and improving its fatigue strength [1]. But, because fatigue strength of the spot welded lap joint is influenced by its geometrical and mechanical factors, welding condition and etc., there needs a quantitative and systematic estimation method of them. In this report, by considering nugget edge of the spot weld part of the spot welded lap joint subjected to tensile load to the ligament crack, fatigue strength of various spot welded lap joints was estimated with the stress intensity factor (S.I.F.) K which is fracture mechanical parameter. It is known that evaluation of fatigue strength of the spot welded lap joint by the stress intensity factor (S.I.F.) K is more effective than the maximum stress $(\sigma_{ymax}$) at edge of the spot weld part on the center line of width of the plate.

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A Study on the Generation of 3 Dimensional Graphic Files Using SPOT Imagery (SPOT 위성영상을 이용한 3차원 그래픽 화일 생성연구)

  • Cho, Bong-Whan;Lee, Yong-Woong;Park, Wan-Yong
    • Journal of Korean Society for Geospatial Information Science
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    • v.3 no.1 s.5
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    • pp.79-89
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    • 1995
  • Using SPOT satellite imagery, 3 dimensional geographic information can be obtained from SPOT's oblique viewing image. Especially, SPOT provides high spatial resolution, adequate base/height ratio and stable orbit characteristics. In this paper, 3D terrain features were extracted using SPOT stereo image and also the techniques for generation of 3D graphic data were developed for the extracted terrain features. We developed computer programs to generate automatically 3D graphic files and to display geographic information on the computer screen, The results of this study may be effectively utilized for the development of 3D geographic information using satellite images.

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