• Journal of Forest and Environmental Science
• /
• 제37권4호
• /
• pp.331-337
• /
• 2021

This study is designed according to the fact that the feces presumed to be from a Prionailurus bengalensis was found in Ulleungdo Island, where Prionailurus bengalensis is not known to inhabit, and that visual observation of the feces may cause errors in species identification. The feces observed in Ulleungdo Island on October 21, 2019 and August 29, 2020, in Gyeongju on December 4, 2020, and in Jecheon on December 7, 2020 was found intactly on grass, not buried in the ground. Although it was difficult to distinguish and identify the feces of Felis catus and Prionailurus bengalensis with visual observation, the feces collected from Ulleungdo Island was closely related to the Felis catus according to the genetic analysis whereas the ones collected from Gyeongju and Jecheon was identified from Prionailurus bengalensis. Therefore through the gene analysis, this study proved that visual observation of feces with similar appearance, specifically the feces found in Ulleungdo Island, Gyeongju, and Jecheon, may cause errors in species identification. It is judged to be necessary to analyze fields signs and genes for the species identification when using the feces of Felis catus and Prionailurus bengalensis.

• 환경생물
• /
• 제41권2호
• /
• pp.145-166
• /
• 2023

Parrots have been threatened by global trade to meet their high demand as pets. Controlling parrot trade is essential because parrots play a vital role in the ecosystem. Accurate species identification is crucial for controlling parrot trade. Parrots have been traded as eggs due to their advantages of lower mortality rates and more accessible transport than live parrots. A molecular method is required to identify parrot eggs because it is difficult to perform identification using morphological features. In this study, DNAs were obtained from 43 unidentified parrot eggs using a non-destructive sampling method. Partial cytochrome b (CYTB) gene was then successfully amplified using polymerase chain reaction (PCR) and sequenced. Sequences newly obtained in the present study were compared to those available in the GenBank by database searching. In addition, phylogenetic analysis was conducted to identify species using available sequences in GenBank along with sequences reported in previous studies. Finally, the 43 parrot eggs were successfully identified as seven species belonging to two families and seven genera. This non-destructive sampling method for obtaining DNA and molecular identification might help control the trade of parrot eggs and prevent their illegal trade.

• 한국가구학회지
• /
• 제12권2호
• /
• pp.39-47
• /
• 2001

This study was carried out to identify wood species excavated at the temple site of Backjae dynasty in Neungsanri, Buyeo. At the temple site, there were great excavations such as Backjae Incense Burner(National Treasure No.287) and Stone Reliquary(National Treasure No.288). The King Chang 13 Year carved on Stone Reliquary indicated that this temple was completed in A.D 567. Identification of wood species were as follows: The Temple building was constructed by Pinus densiflora Sieb. et Zucc. The species of the main pillar of Wooden Pagoda which preserved relics of the Buddha was Zelkova serrate Makino.

• International Journal of Oral Biology
• /
• 제31권1호
• /
• pp.11-14
• /
• 2006

This study was undertaken to develop PCR primers for the identification and detection of Streptococcus anginosus using species-specific forward and reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene(rDNA). The primer specificity was tested against 12 S. anginosus strains and 6 different species(10 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. anginosus ATCC $33397^T$. The data showed that species-specific amplicons were obtained from all the S. anginosus strains tested, but not in the six other species. The PCR could detect as little as 0.4pg of the chromosomal DNA from S. anginosus. This suggests that the PCR primers are highly sensitive and applicable to the detection and identification of S. anginosus.

• 한국수산과학회지
• /
• 제50권2호
• /
• pp.195-206
• /
• 2017

Joint time-frequency images of the broadband echo signals of six fish species were obtained using the smoothed pseudo-Wigner-Ville distribution in controlled environments. Affine transformation and principal component analysis were used to obtain eigenimages that provided species-specific acoustic features for each of the six fish species. The echo images of an unknown fish species, acquired in real time and in a fully automated fashion, were identified by finding the smallest Euclidean or Mahalanobis distance between each combination of weight matrices of the test image of the fish species to be identified and of the eigenimage classes of each of six fish species in the training set. The experimental results showed that the Mahalanobis classifier performed better than the Euclidean classifier in identifying both single- and mixed-species groups of all species assessed.

• Journal of Species Research
• /
• 제9권3호
• /
• pp.288-323
• /
• 2020

A revised checklist of Korean Trichoptera is provided for the species recorded from the Korean Peninsula, including both North and South Korea. The checklist includes bibliographic research as well as results after reexamination of some specimens. For each species, we provide the taxonomic literature that examined Korean Trichoptera materials or mentioned significant taxonomic treatments regarding to Korean species. We also provide the records of unnamed species based on larval identification for further study. Based on taxonomic considerations, 20 species among the previously known nominal species in Korea are deleted or synonymized, and three species omitted from the previous lists, Hydropsyche athene Malicky and Chantaramongkol, 2000, H. simulata Mosely, 1942 and Helicopsyche coreana Mey, 1991 are newly added to the checklist. Hydropsyche formosana Ulmer, 1911 is recorded from the Korean Peninsula for the first time by the identification of Hydropsyche KD. In addition, we recognized 14 species of larvae separated with only tentative alphabetic designations. As a result, this new Korean Trichoptera checklist includes 218 currently recognized species in 66 genera and 25 families from the Korean Peninsula.

• 한국수산과학회지
• /
• 제55권5호
• /
• pp.742-750
• /
• 2022

This study aimed to monitor the distribution of Cobitidae in Korea by the identification of species using genetic analysis. Based on the genetic analysis, Cobitidae species in four of five domestic fish farms consisted of only Chinese muddy loach Misgurnus mizolepis, but muddy loach Misgurnus anguillicaudatus was also present it in one fish farm. In the case of imported Cobitidae species, in addition to Chinese muddy loach and muddy loach, the harmful species Paramisgurnus dabryanus, was also present. Chinese muddy loach accounted for 20%, 67%, and 60% of the S6, S7, and S8 samples, respectively. An analysis of the total length, body length, and weight showed that domestic Chinese muddy loach showed higher values than imported muddy loach, and imported Chinese muddy loach showed similar values to P. dabryanus. There were no significant differences in the country of origin of the three species. Thus, the mitochondrial cytochrome c oxidase subunit I gene sequence was analyzed and compared the verification of species identification. The three species of Cobitidae were genetically divided into three groups and determined to have genetic differences. These results indicate that it is necessary to reduce the heterogeneous mixing rate through discriminating species by genetic analysis.

• Animal Systematics, Evolution and Diversity
• /
• 제36권1호
• /
• pp.60-77
• /
• 2020

Cecidomyiidae (Diptera) consists of six subfamilies, which are divided into three groups according to larval ecological habits (phytophagous, mycophagous, and zoophagous). The five basal subfamilies of Cecidomyiidae consist entirely of mycophagous species, with approximately 1500 species described worldwide and 29 previously known to occur in Korea. In this study, 37 named species (1 Lestremiinae, 29 Micromyinae, 4 Winnertziinae, and 3 Porricondylinae species) are newly reported from South Korea. We excluded Lestremia yasukunii Shinji from the list of Korean mycophagous cecidomyiids as it is a nomen nudum. Therefore, we herein officially recognize 65 species, 30 genera, and four subfamilies for the Korean mycophagous cecidomyiid fauna. We also provide diagnoses and photographs to aid species identification and discussion on the four years of gall midge taxonomic inventory in South Korea.

• Journal of Microbiology
• /
• 제41권4호
• /
• pp.312-319
• /
• 2003

In an attempt to develop a method for rapid and accurate identification of six Vibrio species that are clinically important and most frequently detected in Korea, 16S rDNA restriction fragment length polymorphism (RFLP) of Vibrio type strains, as well as environmental isolates obtained from the Korean coastal area, was analyzed using ten restriction endonucleases. Digestion of the 16S rDNA fragments amplified by polymerase chain reaction (PCR) with the enzymes gave rise to 2~6 restriction patterns for each digestion for 47 Vibrio strains and isolates. An additional 2~3 restriction patterns were observed for five reference species, including Escherichia coli, Aeromonas hydrophila, A. salmonicida, Photobacterium phosphoreum, and Plesiomonas shigelloides. A genetic distance tree based on RFLP of the bacterial species correlated well with that based on 16S rDNA sequences. The very small 16S rDNA sequence difference (0.1%) between V. alginolyticus and V. parahaemolyticus was resolved clearly by RFLP with a genetic distance of more than 2%. RFLP variation within a species was also detected in the cases of V. parahaemolyticus, V. proteolyticus, and V. vulnificus. According to the RFLP analysis, six Vibrio and five reference species were assigned to 12 genotypes. Using three restriction endonucleases to analyze RFLP proved sufficient to identify the six pathogenic Vibrio species.

• Genomics & Informatics
• /
• 제10권3호
• /
• pp.206-211
• /
• 2012

DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects, and shellfish. We present results on phylogenetic relationships to assess biodiversity the in the Korean peninsula. Average GC% contents of the 68 fish species (46.9%), the 59 shellfish species (38.0%), and the 29 insect species (33.2%) are reported. Using the Kimura 2 parameter in all possible pairwise comparisons, the average interspecific distances were compared with the average intraspecific distances in fish (3.22 vs. 0.41), insects (2.06 vs. 0.25), and shellfish (3.58 vs. 0.14). Our results confirm that distance-based DNA barcoding provides sufficient information to identify and delineate fish, insect, and shellfish species by means of all possible pairwise comparisons. These results also confirm that the development of an effective molecular barcode identification system is possible. All DNA barcode sequences collected from our study will be useful for the interpretation of species-level identification and community-level patterns in fish, insects, and shellfish in Korea, although at the species level, the rate of correct identification in a diversified environment might be low.