• 대한전자공학회논문지SP
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• 제38권5호
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• pp.548-555
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• 2001

본 논문에서는 인간시각시스템 모델에 기반하여 회전과 이동은 물론 유손실 압축과 잘라내기 및 신축에 대해서도 견고한 디지털 영상 워터마킹을 제안하였다. 기존의 퓨리에 변환과 로그폴라맵핑을 이용하여 회전및 이동에 불변하도록함과 동시에 인간시각스템 모델의 공간주파수 감도를 감안하여 비가시성이 떨어지지 않는 범위 내에서 워터마크 에너지를 최대한 인가하고, 각 워터마크 비트들의 정보를 임의 섞음하여 전 영역에 분산 삽입하였다. 이와 같이 함으로써 잘라내기와 유손실 압축과 같은 영상처리나 회전, 이동 및 신축과 같은 기하학적 변형 등에 대해 워터마크 견고성을 더욱 향상시켰다. 또한 워터마크와 템플릿을 교차하지 않게 삽입함으로써 이들의 교차로 인한 비가시성과 견고성의 저하를 방지하였다. 실험을 통하여 제안한 워터마킹 방법은 유손실 압축, 잘라내기 및 신축에 대하여 기존방법보다 약 30∼75 [%]정도 더 견고성이 향상됨을 확인하였다.

• 한국식품과학회지
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• 제15권1호
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• pp.51-55
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• 1983

전분질로부터 효모균체를 직접 생산하기 위하여 분리 동정된 전분이용성 효모, Sp. holsaticus FRI Y-5의 기본적인 배양조건에 대하여 검토하였다. 이 균주는 산소를 절대적으로 요구하며, 배지의 초기 pH가 6.9일 때 최대균체량 7.2 g/l, 균체수율 0.27, 비성장속도 $0.14hr^{-1}$로 가장 좋은 생육현상을 보여 주었고 배양온도는 $23^{\circ}C$가 적온이었다. 균체생육의 활성화 에너지 Ea는 17.33 kcal/mole로 산출되었다. 검토된 13종의 질소원 가운데 $(NH_2)_2CO$가 3일 배양후 균체량이 10.69 g/l로 가장 많았으며 이때 균체수율은 0.451이었다. $SO_4\;^{-2}$염으로서 $Mg^{+2},\;K^+$ 및 $Zn^{+2}$와 유기영양원으로서 yeast extract도 균체증식에 좋은 영향을 주는 것으로 판명되었다.

• 대한기계학회논문집A
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• 제26권8호
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• pp.1577-1584
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• 2002

This paper develops a finite element model for studying the occupant behavior and injury cofficients of a large-sized cab-over type truck. Since it does not have a room to absorb collision energy and deformation in front of the passenger compartment the deformation is directly transmitted to the passenger compartment. Moreover, since its steering column is attached on the frame, severe deformation of the frame directly affects on the steering wheel's movement. Therefore, if the occupant behavior and injury coefficients analysis is performed using a finite element model developed based on a sled test, it is very difficult to expect acquiring satisfactory results. Thus, the finite element model developing in this paper is based on the frontal crash test in order to overcome the inherent problems of the sled test based model commonly used in the passenger car. The occupant behavior and injury coefficients analysis is performed using PAM-CRASH installed in super-computer SP2. In order to validate the reliability of the developed finite element model, a frontal crash test is carried out according to a test method used fur developing truck occupant's secondary safety system in european community and japan. That is, test vehicle's collision direction is vertical to the rigid barrier and collision velocity is 45kph. Thus, measured vehicle pulses at the lower parts of the left and right B-pilla., dummy chest and head deceleration profiles, HIC(head injury criterial) and CA(chest acceleration) values, and dummy behavior from the frontal crash test are compared to the analysis results to validate reliability of the developed model.

• 한국토양비료학회지
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• 제47권6호
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• pp.391-397
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• 2014

Acid mine drainage occurrence is a serious environmental problem by mining industry; it usually contain high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of greatest concern. It causes mine impacted soil pollution with mining and smelting activities, fossil fuel combustion, and waste disposal. In the present study, three bacterial strains capable of producing urease were isolated by selective enrichment of heavy metal contaminated soils from a minei-mpacted area. All isolated bacterial strains were identified Sporosarcina pasteurii with more than 98% of similarity, therefore they were named Sporosarcina sp. KM-01, KM-07, and KM-12. The heavy metals detected from the collected mine soils containing bacterial isolates as Mn ($170.50mg\;kg^{-1}$), As ($114.05mg\;kg^{-1}$), Zn ($92.07mg\;kg^{-1}$), Cu ($62.44mg\;kg^{-1}$), and Pb ($40.29mg\;kg^{-1}$). The KM-01, KM-07, and KM-12 strains were shown to be able to precipitate calcium carbonate using urea as a energy source that was amended with calcium chloride. SEM-EDS analyses showed that calcium carbonate was successfully produced and increased with time. To confirm the calcium carbonate precipitation ability, urease activity and precipitate weight were also measured and compared. These results demonstrate that all isolated bacterial strains could potentially be used in the bioremediation of acidic soil contaminated by heavy metals by mining activity.

• 미생물학회지
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• 제18권1호
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• pp.20-28
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• 1980

1. 꽃에서 분리한 200여 균주에서 30종을 동정하였으며, 그 중 메탄올을 자화할수있는 균주는 23종이었으며, 가장 생장이 좋은 3종을 선별하였다. 그 3종은 Candida incommunis, Cryptococcus aerius, Haηsenula ciferrii였다. 2. 위의 3종은 복합비타민과 yeast extract의 공급이 없이 탄소원으로 메탄올을 자화하여 중식하였으며, 메탄올을 이용하여 증식한 효모의 아미노산 조성을 검사한 결과 필수 아미노산이 모두 포함되어 있었다. 3. 총 아마노산의 함량이 Candida incommunis는 42.5%, Hansenula ciferrii는 39.9%로서 Kloeckera sp. No. 22 (Ogata 둥 1910a)의 39.9%보다 높고, Cryptococcus aeriuS는 36.5%로서 Candida utilis(Ogata 등 1970b)의 31.9%보다 높다. 4.곡류단백질에 흔히 결핍되어 있는 lysine과 threonine의 필수 아미노산이 이들 yeast에서는 소액분의 수준으로 포함되어 있다. 5. 총 질소량 Candida incommunis가 31.4%, Hansenula ciferrii가 28.8%, Cryptococcus aerius가 27.1%로 나타났다. 6. 높은 균체수율을 위한 실험결과 메탄올의 농도 1.3%, 온도 $30^{\circ}C$가 최적 생장조건으로 나타났다.

• 콘크리트학회논문집
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• 제17권1호
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• pp.113-120
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• 2005

본 연구의 목적은 기둥의 내진성능을 개선하기 위한 연구로서, 섬유-강판이 복합된 보강재를 사용하여 보강기둥의 내력특성을 연구하였다. 이용 가능한 복합판의 보강량을 선정하고, 기존의 탄소섬유와 강판으로 보강한 기둥과 함께 밴드형 보강실험체를 제작하여 일정축력을 받는 기둥에 반복횡하중을 가하여 하중, 변의 및 연성도를 조사하였다. 내진성능의 척도가 되는 변위연성도, 에너지연성도, 누적소산에너지 및 감쇠비를 비교하여 복합판을 사용한 부재의 내력특성을 평가하였다. 연구결과, 복합판을 사용하여 기둥을 보강할 경우에도 다른 보강방법과 같이 양호한 내진성능을 확보할 수 있었다. 또한, 밴드형식으로 보강한 보강실험체의 연성능력은 모두 양호한 편이나, 최대하중 증가를 위해서는 일정량 이상의 보강량이 필요한 것으로 나타났다. 연성도 평가로는 에너지연성도가 변위연성도에 비하여 보강기둥의 연성능력을 잘 나타내었다.

• 한국환경과학회지
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• 제22권1호
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• pp.91-98
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• 2013

In order to study the seasonal patterns and possible origins of air concentrations of volatile organic compounds(VOC), measurements were taken with GC-MS at 3 sampling sites in Jinju for 12 months from Mar. 2010 to Feb. 2011. Atmospheric VOC are sampled on tubes containing solid adsorbents(Tenax TA) with a time resolution of 2hrs. Composition and concentration of VOC are analysed with a GC system equipped with thermal desorption apparatus(ATD). The most abundant compound appeared to be Toluene, Ethylbenzene and m,p-Xylene. The mean concentrations of Benzene were 0.20 ppb at GN site, 0.18 ppb at DA site, and 0.25 ppb at SP site, respectively. VOC concentration showed a strong seasonal variation, with higher concentrations during the spring and lower concentrations during the summer. The results showed that monthly fluctuations in measured VOC concentrations depended on variations in the strength of sources, as well as on photochemical activity and meteorological conditions. In Jinju, the total VOC emissions for 2009 were estimated to be 4,407 ton/year by Clean Air Policy Support System(CAPSS). It is shown that solvent use 57.5%(2,534 ton/yr), waste treatment and disposal 23.3%(1,025 ton/yr), and mobil source-road traffic 12.2%(537 ton/yr) are the most significant anthropogenic source.

• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.1019-1027
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• 2009

A noncompartmented microbial fuel cell (NCMFC) composed of a Mn(IV)-carbon plate and a Fe(III)-carbon plate was used for electricity generation from organic wastewater without consumption of external energy. The Fe(III)-carbon plate, coated with a porous ceramic membrane and a semipermeable cellulose acetate film, was used as a cathode, which substituted for the catholyte and cathode. The Mn(IV)-carbon plate was used as an anode without a membrane or film coating. A solar cell connected to the NCMFC activated electricity generation and bacterial consumption of organic matter contained in the wastewater. More than 99% of the organic matter was biochemically oxidized during wastewater flow through the four NCMFC units. A predominant bacterium isolated from the anode surface in both the conventional and the solar cell-linked NCMFC was found to be more than 99% similar to a Mn(II)-oxidizing bacterium and Burkeholderia sp., based on 16S rDNA sequence analysis. The isolate reacted electrochemically with the Mn(IV)-modified anode and produced electricity in the NCMFC. After 90 days of incubation, a bacterial species that was enriched on the Mn(IV)-modified anode surface in all of the NCMFC units was found to be very similar to the initially isolated predominant species by comparing 16S rDNA sequences.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1221-1228
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• 2013

Two lipase genes (bpl1 and bpl3) from Antarctic Bacillus pumilus strains were expressed in Bacillus subtilis. Both recombinant lipases BPL1 and BPL2 were secreted to the culture medium and their activities reached 3.5 U/ml and 5.0 U/ml, respectively. Their molecular masses apparent using SDS-PAGE were 23 kDa for BPL1 and 19 kDa for BPL3. Both lipases were purified to homogeneity using ammonium sulfate precipitation and HiTrap SP FF column and Superose 12 column chromatographies. The final specific activities were estimated to be 328 U/mg for BPL1 and 310 U/mg for BPL3. Both lipases displayed an optimum temperature of $35^{\circ}C$, similar to other mesophilic enzymes. However, they maintained as much as 70% and 80% of the maximum activities at $10^{\circ}C$. Accordingly, their calculated activation energy at a temperature range of $10-35^{\circ}C$ was 5.32 kcal/mol for BPL1 and 4.26 kcal/mol for BPL3, typical of cold-adapted enzymes. The optimum pH of BPL1 and BPL3 was 8.5 and 8.0, respectively, and they were quite stable at pH 7.0-11.0, showing their strong alkaline tolerance. Both lipases had a preference toward medium chain length ($C_6-C_{10}$) fatty acid substrates. These results indicate the potential for the two Antarctic B. pumilus lipases as catalysts in bioorganic synthesis, food, and detergent industries.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1580-1587
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• 2012

Japanese encephalitis virus (JEV) envelope (E) protein holds great promise for use in the development of a recombinant vaccine. Purified recombinant E (rE) protein may be useful for numerous clinical applications; however, there are limitations in using the Escherichia coli expression system for producing high-quality rE protein. Therefore, in this study, the yeast expression system was used to generate the rE protein. For protein production using the yeast system, the full-length JEV E gene was cloned into Pichia pastoris. SDS-PAGE and immunoblotting analysis demonstrated that the rE protein had a molecular mass of 58 kDa and was glycosylated. The predicted size of the mature unmodified E protein is 53 kDa, suggesting that post-translational modifications resulted in the higher molecular mass. The rE protein was purified to greater than 95% purity using combined ammonium sulfate precipitation and a SP-Sepharose Fast Flow column. This purified rE protein was evaluated for immunogenicity and protective efficacy in mice. The survival rates of mice immunized with the rE protein were significantly increased over that of Hyphantria cunea nuclear polyhedrosis virus E protein (HcE). Our results indicate that the rE protein expressed in the P. pastoris expression system holds great promise for use in the development of a subunit vaccine against JEV.