• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.117-123
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• 2003

For screening of autoregulator receptor gene from Saccharopolyspora erythraea, PCR was performed with primers of receptor gene designed on the basis of amino acid sequences of autoregulator receptor proteins with known function. PCR products were subcloned into the BamHI site of pUC19 and transformed into the E. coli DH5$\alpha$. The isolated plasmid from transformant contained the fragment of 120 bp, which was detected on 2% gel after BamHI treatment. The insert, 120 bp PCR product, was confirmed as the expected internal segment of gene encoding autoregulator receptor protein by sequencing. Southern and colony hybridization using Saccha. erythraea chromosomal DNA were performed with the insert as probe. The plasmid (pEsg) having 3.2 kbp SacI DNA fragment from Saccha. erythraea is obtained. The 3.2 kbp SacI DNA fragment was sequenced by the dye terminator sequencing. The nucleotide sequence data was analyzed with GENETYX-WIN (ver 3.2) computer program and DNA database. frame analyses of the nucleotide sequence revealed a gene encoding autoregulator receptor protein which is a region including KpnI and SalI sites on 3.2 kbp SacI DNA fragment. The autoregulator receptor protein consisting of 205 amino acid was named EsgR by author. In comparison with known autoregulator receptor proteins, homology of EsgR showed above 30%.

• The Korean Journal of Physiology and Pharmacology
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• v.10 no.4
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• pp.217-222
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• 2006

Atherosclerosis is considered as a chronic inflammatory process. However, the nature of the oxidant signaling that regulates monocyte adhesion and its underlying mechanism is poorly understood. We investigated the role of reactive oxygen species on the vascular cell adhesion molecule-1 (VCAM-1) and monocyte adhesion in the cultured endothelial cells. $TNF-{\alpha}$ at a range of $1{\sim}30\;ng/ml$ induced VCAM-1 expression dose-dependently. BCECF-AM-labeled U937 cells firmly adhered on the surface of endothelial cells when the endothelial cells were incubated with $TNF-{\alpha}$ (15 ng/ml). Ten $\;{\mu}mol/L$ of SB203580, an inhibitor of p38 MAPK, significantly reduced $TNF-{\alpha}-induced$ VCAM-1 expression, compared to the JNK inhibitor ($40\;{\mu}mol/L$ of SP60015) or ERK inhibitor ($40\;{\mu}mol/L$ of U0126). Also, SB203580 significantly inhibited $TNF-{\alpha}-induced$ monocyte adhesion in HUVEC. Superoxide production was minimal in the basal condition, however, treatment of $TNF-{\alpha}$ induced superoxide production in the dihydroethidineloaded endothelial cells. Diphenyleneiodonium (DPI, $10\;{\mu}mol/L$), an inhibitor of NADPH oxidase, and rotenone $(1\;{\mu}mol/L)$, an inhibitor of mitochondrial complex I inhibited $TNF-{\alpha}-induced$ superoxide production, VCAM-1 expression and monocyte adhesion in the endothelial cells. Taken together, our data suggest that NADPH oxidase and mitochondrial ROS were involved in $TNF-{\alpha}-induced$ VCAM-1 and monocyte adhesion in the endothelial cells.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.42 no.3 s.303
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• pp.73-80
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• 2005

Recently there have been many efforts to support searching and browsing based on the visual content of image and multimedia data. Most existing approaches to content-based image retrieval rely on query by example or user based low-level features such as color, shape, texture. But these methods of query are not easy to use and restrict. In this paper we propose a method for automatic color object extraction and labelling to support multiple queries of content-based image retrieval system. These approaches simplify the regions within images using single colorizing algorithm and extract color object using proposed Color and Spatial based Binary tree map(CSB tree map). And by searching over a large of number of processed regions, a index for the database is created by using proposed labelling method. This allows very fast indexing of the image by color contents of the images and spatial attributes. Futhermore, information about the labelled regions, such as the color set, size, and location, enables variable multiple queries that combine both color content and spatial relationships of regions. We proved our proposed system to be high performance through experiment comparable with another algorithm using 'Washington' image database.

• Journal of the Korean Chemical Society
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• v.4 no.1
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• pp.15-17
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• 1957

1. Preparation of Titanium tetrachloride; The following precesses were strictly followed as the preliminary step to obtain pure $TiOCl_2$, titanyl chloride; First, pure Titanium Oxide mixed with carbon is rolled into pills. After drying up perfectly, these pills are heated at 900∼1000${\circ}C$. And then the pills are subjected to the flow of $Cl_2$ gas in a quartz tube heated to 900-1000${\circ}C$. Thus Titanium tetrachloride is obtained. 2. Preparation of $TiOCl_2$ ; Yellowish trobrown solution is made by pouring 80 g of conc. HCl (sp.gr. 1.19) to 45 gr of Titanium tetrachloride (approx. 2 times of theoretical amount). Then this solution is kept settled for 5-days in a desiccator filled with phosphorous pentoxide at room temperature. As the colorless amorphous solid thus obtained is washed with aceton, 36.5 g of the pure salt are obtained. 3. Determination of composition. The analysis of the sample taken from the deposit desiccated gives the following data; (A) Qualitative analysis; a) $Ti(OH)_4$ is precipitated by adding NaOH in water solution of the salt. b) Adding $AgNO_3$ solution, the water solution of the salt gives white precipitate of AgCl. c) When acid and $H_2O_2$ are added, the solution turns its color to redish brown (This proves that $TiO^{++}$ was converted into $TiO^{++}$ by oxidation of $H_2O_2$. (B) Quantitative analysis; a) $Ti(OH)_4$ precipitated by $10{\%}$ NaOH isalitatsubjected consecutively to the filtration and ignition in porcelain crucible at approx. 1000${\circ}C$. , then $TiO_2$ thus formed is weighed and calculated into Ti content. b) Chlorine involved in water solution of the salt is determined by Vorhardt method. Result: The values obtained from previous analysis, devied by their atomic weight gives the following composition: Ti : Cl = 1 : 2 Therefore $TiOCl_2$ should be given as its molecular formula. 4. Summary. When $TiCl_4$ is additated into conc. HCl, $TiO^{++}$ formed exists as a stable form, and forms $TiOCl_2$. However $TiOCl_2$ is unstable to heating. When the temperature is raised to $65{\circ}C$the decomposition of the solution is accelerated, and gives $TiO_2$ aq. $TiOCl_2$ in addition is highly hygroscopic.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.37 no.1
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• pp.27-36
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• 2000

As a potential approach for non-destructive testing of concrete structures, we evaluate the time-of-flight (TOF) ultrasound tomography technique In conventional X ray tomography, the reconstructed Image corresponds to the internal attenuation coefficient However, in TOF ultrasound tomography, the reconstructed Image is proportional to the retractive index of the medium Because refractive effects are minimal for X-rays, conventional reconstruction techniques are applied to reconstruct the Image in X-ray tomography However, since ultrasound travels in curved path, due to the spatial variations in the refractive index of the medium, the path must be known to correctly reconstruct the Image. Algorithm for determining the ultrasound path is developed from a Geometrical Optics point view and the image reconstruction algorithm, since the paths are curved It requires the algebraic approach, namely the ART or the SIRT Here, the difference between the computed and the measured TOP data is used as a basis, for the iteration process First the initial image is reconstructed assuming straight paths. It then updates the path based on the recently reconstructed image This process of reconstruction and path determination repeats until convergence The proposed algorithm is evaluated by computer simulations, and in addition is applied to a real concrete structure.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.5
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• pp.25-33
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• 2010

Multi-media service has been changed into user based audio services, which service supports actively user's preference and interaction with the users. In the market, multi-media products which can support the highest audio-quality by using lossless audio technology have been released and object audio music which user can select the objects has been serviced. In this paper, we design user's preference information based object audio file format and audio scene description for storage and transmission media. The designed file format is designed based on MPEG-4 file format because high-quality audio codecs in MPEG-4 audio can be easily used and the track of file format can be flexibly controlled depend on the number of the instrument in music. The encoded audio data of each objects and encoded audio scene description by binary encoding that has independent track are packed in a file. The scene description for storage media is consist of full and object scene description, the scene description for transmission media has an essential description for object audio operation and a specific description for real audio sound. The designed file format based simulator is developed and it generates an object audio file with several scene descriptions. Also, the real audio sound is serviced by the interaction with user and the unpacked scene description.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.43 no.1 s.307
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• pp.27-38
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• 2006

The main purpose of rate control is to achieve the highest video quality when bandwidth or storage capacity is limited. For this purpose, we need a rate control algorithm which is adaptively controlled by the motion information of sequences, scene change, buffer capacity and time-varing bandwitdh channels. A rate-control method in the encoder requires the accurate estimation of target bit for each frame and the low end-to-end delay for transmitting video data by intelligent selection of encoding parameters. In this paper, we suggest three kinds of linear relation in the encoder to satisfy the characteristics of rate control. The first relation is that between the percentage of zero quantized transformed coefficients(p) and coded bits. Second relation is that between the PSNR of encoded frame and its Quantization parameter(QP). Finally, we can find out a linear approximation between QP and p. According to the experimental analysis, the proposed method results in an efficient rate control in terms of the bit estimation, the buffer capacity, and PSNR compared with the existing rate control in the H.264 JM 9.3.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.41 no.5
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• pp.45-52
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• 2004

According tothe development of digital media technologies various algorithms for video sequence matching have been proposed to match the video sequences efficiently. A large number of video sequence matching methods have focused on frame-wise query, whereas a relatively few algorithms have been presented for video sequence matching or video shot matching. In this paper, we propose an efficientalgorithm to index the video sequences and to retrieve the sequences for video sequence query. To improve the accuracy and performance of video sequence matching, we employ the Cauchy function as a similarity measure between histograms of consecutive frames, which yields a high performance compared with conventional measures. The key frames extracted from segmented video shots can be used not only for video shot clustering but also for video sequence matching or browsing, where the key frame is defined by the frame that is significantly different from the previous fames. Several key frame extraction algorithms have been proposed, in which similar methods used for shot boundary detection were employed with proper similarity measures. In this paper, we propose the efficient algorithm to extract key frames using the cumulative Cauchy function measure and. compare its performance with that of conventional algorithms. Video sequence matching can be performed by evaluating the similarity between data sets of key frames. To improve the matching efficiency with the set of extracted key frames we employ the Cauchy function and the modified Hausdorff distance. Experimental results with several color video sequences show that the proposed method yields the high matching performance and accuracy with a low computational load compared with conventional algorithms.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.10 no.1
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• pp.31-36
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• 1977

The monthly distribution of marine bacteria in the coastal waters of Chung-Mu was investigated from April, 1976 to March, 1977. The aim of this study was to obtain basic data for the prevention of food poisoning and for the efficient aquaculture of the area. Samples of sea water, mud, fish and shell fish were taken every month. The results are as follows: 1. One thousand four hundred and twenty-six strains were isolated from 732 samples of sea water, mud, fish and shell fish. They were 450 strains of Pseudomonas fluorescens, 422 strains of Achromobacter liquefacience, 72 strains of Vibrio parahaemolyticus, 234 strain of Vibrio alginolyticus, and 248 strains of Proteus vulgaris. 2. V. parahaemolyticus occupied $9.84\%$ of the total samples and $52\%$ of them were found in the sea water and mud. 3. The muds sampled districtly beneath the aquaculture raft contained much gas. They seemed to originate from the deposition of excretion of shell fish. The fatness of the shell fish was low where the gas was abundant. 4. It was .found that the shell fish with low fatness contained much Vibrio sp. 5. Regional distribution shows that marine bacteria were abundant in the order of station 10, 9, 8, 11, 1. The area around station 9 was polluted by reclamation of the area, and station 8 showed the influence of the excrement treat tank located nearly.

• BMB Reports
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• v.40 no.3
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• pp.315-324
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• 2007

The novel $\alpha$-amylase purified from locally isolated strain, Bacillus sp. KR-8104, (KRA) (Enzyme Microb Technol; 2005; 36: 666-671) is active in a wide range of pH. The enzyme maximum activity is at pH 4.0 and it retains 90% of activity at pH 3.5. The irreversible thermoinactivation patterns of KRA and the enzyme activity are not changed in the presence and absence of $Ca^{2+}$ and EDTA. Therefore, KRA acts as a $Ca^{2+}$-independent enzyme. Based on circular dichroism (CD) data from thermal unfolding of the enzyme recorded at 222 nm, addition of $Ca^{2+}$ and EDTA similar to its irreversible thermoinactivation, does not influence the thermal denaturation of the enzyme and its Tm. The amino acid sequence of KRA was obtained from the nucleotide sequencing of PCR products of encoding gene. The deduced amino acid sequence of the enzyme revealed a very high sequence homology to Bacillus amyloliquefaciens (BAA) (85% identity, 90% similarity) and Bacillus licheniformis $\alpha$-amylases (BLA) (81% identity, 88% similarity). To elucidate and understand these characteristics of the $\alpha$-amylase, a model of 3D structure of KRA was constructed using the crystal structure of the mutant of BLA as the platform and refined with a molecular dynamics (MD) simulation program. Interestingly enough, there is only one amino acid substitution for KRA in comparison with BLA and BAA in the region involved in the calcium-binding sites. On the other hand, there are many amino acid differences between BLA and KRA at the interface of A and B domains and around the metal triad and active site area. These alterations could have a role in stabilizing the native structure of the loop in the active site cleft and maintenance and stabilization of the putative metal triad-binding site. The amino acid differences at the active site cleft and around the catalytic residues might affect their pKa values and consequently shift its pH profile. In addition, the intrinsic fluorescence intensity of the enzyme at 350 nm does not show considerable change at pH 3.5-7.0.