• Title/Summary/Keyword: SOT 배지

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Pilot-scale preparation and physicochemical characteristics of microbiological agar from Gelidium amansii in Korea (국내산 우뭇가사리로부터 미생물 배지용 한천의 pilot규모 정제와 특성)

  • KIM Doo-Sang;KIM Hyeung-Rak;KIM Jeong-Han;PYEUN Jae-Hyeong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.1
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    • pp.70-74
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    • 2000
  • Agar for microbiological medium was prepared with pilot-scale for industrial application by the process of microfiltration ($0.4 {\mu}m\;pore size$) in $40{\~}50{\circ}C$, washing with sot water, and treatment with $0.25\;N NaOH\;at\;70{\circ}C$. Transparency, gel strength, viscosity, sulfate content, and syneresis ratio of agar prepared from Gelidium amansii was compared with commercial agar for microbiological medium. Gel strength and transparency were increased with processing, however, it's viscosity, sulfate content, and syneresis ratio were reduced. The final agar product was superior to commercial agar for microbiological medium.

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Growth and r-Linolenic Acid Production of Arthrospira (Spirulina) platensis in Heterotrophic Culture. (Arthrospira (Spirulina) platensis의 종속영양배양과 r-Linolenic Acid 생산)

  • Choi, Gang-Guk;Bae, Myoung-Sook;Park, Je-Seop;Park, Bok-Jun;Ahn, Chi-Yong;Oh, Hee-Mock
    • Microbiology and Biotechnology Letters
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    • v.35 no.1
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    • pp.45-51
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    • 2007
  • Arthrospira (Spirulina) platensis is one of the commercially important filamentous cyanobacteria. The heterotrophic cultivation of Arthrospira can be an alternative strategy for commercial mass production. In heterotrophic culture, the specific growth rate of A. platensis M9108, a glucose-resistant mutant of A. platensis PCC 9108, was $0.014h^{-1}$ which was 1.8 higher than that oi the previous report. The mutant possessed the facility to assimilate and to metabolize glucose efficiently under heterotrophic condition. However, the r-linolenic acid content of 6 Arthrospira strains was not increased in heterotrophic culture. Four Arthrospira strains out of 6 tested strains were able to utilize maltose as a carbon source under heterotrophic condition. The biomass production of these strains on maltose was similar to that on glucose. The specific growth rate of A. platensis M9108 increased with glucose concentration up to 5.0 g/L and then decreased at a glucose concentration of 10.0 g/L. Additionally, A. platensis M9108 under heterotrophic condition showed no aggregation during the cultivation in contrast to A. platensis PCC 9108.