• Title/Summary/Keyword: SOP

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A Study on Model for Developing SOP with Public Safety Communications (재난통신 표준운영절차(SOP) 개발 모델 연구)

  • Park, Seong-Gyoon
    • Journal of IKEEE
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    • v.23 no.1
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    • pp.214-223
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    • 2019
  • This paper presents methodologies and strategies for developing standard operating procedures (SOPs) that are essential for practical use of disaster communication systems. Therefore, the various factors related to the SOP of the public safety network(SafeNet) are identified and analyzed, and the processes and strategies for the effective development of the SOP are developed and presented based on the analysis. In addition, the proposed SOP is reflected in LTE, which is public safety network implementation technology, and suggests how priority and QoS that are the basis of radio resource allocation should be operated.

Localization of F plasmid SopB protein and Gene silencing via protein-mediated subcellular localization of DNA

  • Kim Sook-Kyung;James C. Wang
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2000.10a
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    • pp.15-23
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    • 2000
  • The subcellular localization of the SopB protein, which is encoded by the Escherichia coli F plasmid and is involved in the partition of the single-copy plasmid, was directly visualized through the expression of the protein fused to the jellyfish green fluorescent protein (GFP). The fusion protein was found to localize to positions close but not at the poles of exponentially growing cells. Examination of derivatives of the fusion protein lacking various regions of SopB suggests that the signal for the cellular localization of SopB resides in a region close to its N terminus. Overexpression of SopB led to silencing of genes linked to, but well-separated from, a cluster of SopB-binding sites termed sopC. In this SopB-mediated repression of sopC-linked genes, all but the N-terminal 82 amino acids of SopB can be replaced by the DNA-binding domain of a sequence-specific DNA -binding protein, provided that the sopC locus is also replaced by the recognition sequence of the DNA-binding domain. These results suggest a mechanism of gene silencing: patches of closely packed DNA-binding protein is localized to specific cellular sites; such a patch can capture a DNA carrying the recognition site of the DNA -binding domain and sequestrate genes adjacent to the recognition site through nonspecific binding of DNA.

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Identification of the Gene Products Responsible for F Plasmid Partitioning

  • Kim, Sung-Uk;Yu, Ju-Hyun;KazuoNagai
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.516.2-516
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    • 1986
  • DNA subfragments, sopA, sopB, and sopC supporting stable maintenance of an oriC plasmid, were derived from mini-F plasmid DNA (EcoRI restriction fragment, f5) after digestion with restriction endonucleases, and cloned in vector plasmid pBR322. The recombinant plasmid obtained were introduced into E. coli KY7231 and E. coli CSR603, and proteins specified by the mini-F fragments were analysed by SDS-polyacrylamide gel electrophoresis. Two proteins encoded by the F fragments were detected, having molecular weights of 41,000 and 37.000. The sopA protein (41K) encoded by a plasmid pXX288 was observed in the cytoplasm, whereas the sopB protein (37K) encoded by a plasmid pXX157 was in the membrane fraction. There was no novel protein band detected in the cell with a plasmid pXX300, which contained sopC fragment. Gene products of a plasmid pXX167, which is comprised of sopA, sopB, and sopC, were not detectable. Fluorography after one and two dimensional gel electrophoresis of the lysates showed that these two proteins were overproduced in the cells which were allowed to incorporate radioactive amino acid after plasmid amplification by chloramphenicol treatment. The isoelectric points of the sopA and sepB proteins were 6.6 and 7.0, respectively.

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A Study on The Awareness of Standard Operating Procedure For The Preparation in Landslide (산사태 대비 SOP에 대한 의식조사 연구)

  • Koo, WonHoi;Shin, HoJoon;Woo, ChoongShik;Baek, MinHo
    • Journal of the Society of Disaster Information
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    • v.9 no.4
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    • pp.503-510
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    • 2013
  • Recently, localized heavy rain is increased by climatic changes and landslide is increased. Also, because of landslide occurred in urban area, life and property damages are increased. Therefore, standard operation procedure of disasters should be established by steps and institutions so as to respond landslide. This thesis investigated application of current disaster manual so as to write SOP of landslide for disaster prevention related experts and accepted opinion for responding necessaries by using landslide SOP and important matters by step of disaster management. As the result of investigation, application of manual was low during the current response to disasters and application was the highest in responding step among the steps of disaster management. In case of landslide, they responded that response with SOP is necessary. During the organization of landslide SOP, they responded that training and education for landslide disaster are important at the step of prevention, conduction of landslide disaster broadcasting and provision of information are important at the step of preparation, guidance for evasion and behavior methods is important at the step of response and investigation of landslide disaster damages and reflection of record and evaluation are important at the step of recovery. In addition, for the requests for landslide SOP application, there was an importance of secondary factors such as expansion of professional manpower, strengthening of law and institution, education and training for SOP acquisition, etc.

Sleep Onset Period from the EEG Point of View (뇌파 영역에서 수면 발생 과정)

  • Lee, Hyun-Kwon;Park, Doo-Heum
    • Sleep Medicine and Psychophysiology
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    • v.16 no.1
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    • pp.16-21
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    • 2009
  • In accordance with the development of EEG and polysomnography in the field of sleep research, the sleep onset period (SOP) between wakefulness and sleep has been considered an important part for understanding the physiology of sleep. SOP in the transition from wakefulness to sleep is a gradual process integrating various viewpoints such as behavior, EEG, physiology and subjective report. Particularly, based on understanding of EEG changes during sleep, SOP has been regarded as a pattern of topographical change in specific frequency and specific state in EEG. Studies on quantitative EEG (qEEG) and event-related potential (ERP) have suggested that SOP shows the changes of functional coordination at the specific cortical areas in qEEG and the changes of regular patterns in response to environmental stimulation in ERP. The development of sleep EEG and topographic mapping of EEG is expected to integrate various viewpoints of SOP and clarify the neurophysiologic mechanism of SOP further.

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Use of the Yeast 1.5-Hybrid System to Detect DNA-Protein-Protein Interaction

  • Kim, Sook-Kyung;Han, Jin-Hee
    • Journal of Microbiology
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    • v.38 no.2
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    • pp.113-116
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    • 2000
  • Escherichia coli F plasmid partition apparatus is composed of two trans-acting proteins (SopA and SopB) and one cis-acting DNA sequence (sopC). The SopB-sopC complex has been suggested to serve a centromere-like function through its interaction with chromosomally encoded proteins which remain to be identified. In this paper, we are introducing a new yeast 1.5-hybrid system which assembles the two-hybrid and one-hybrid system as a mean to find and additional component of the F plasmid partition system, interacting with DNA (sopC)-bound SopB protein. The results indicates that this system is a promising one, capable of selecting an interacting component.

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State Of Polarization Tracking Algorithm using Three-Point Measurement Technique for Polarization Mode Dispersion Compensation (펀광모드분산 보상을 위한 세 점 측정 방식의 빛의 편광상태 추적 알고리즘)

  • 송홍석;정현수;신서용
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.27 no.2B
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    • pp.171-177
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    • 2002
  • As speed and capacity of optical communication system increase dramatically, polarization-mode-dispersion compensation(PMDC) becomes a hot issue in these days. In this paper, we introduce a new state-of-polarization (SOP) tracking algorithm that can be used in a PMDC system. The new SOP tracking algorithm is also based on optical heterodyne coherent detection technique like the previous algorithm that we have reported before. However, unlike the previous algorithm, the new algorithm requires only three times of measurement in any circumstances to find the exact SOP information so that it can effectively be applied to PMDC system where very fast and stable operation is indispensible.

A Feed-forward Microsecond Level Real-time SOP Finding System (순방향 마이크로초 단위의 실시간 편광상태 검출 시스템)

  • Jung, Hyun-Soo;Shin, Seo-Yong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.1C
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    • pp.94-101
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    • 2008
  • In this paper, we introduce a real-time state-of-polarization(SOP) finding system. The system divides the optical wave into linear horizontal- and vertical-SOP components and measures two different beat-signals, which are produced by superposition with reference optical source, in time domain. From these measured beat signals we can get SOP information of the signal instantly. Since the proposed scheme is a feed-forward measurement system, comparing with conventional systems which require an optical feedback loop, the measurement time becomes reduced tremendously. We also introduced a novel calibration method for compensating birefringence-related errors which may occur during the measurement. We prove the operation and performance of the proposed system through computer simulation and actual experiments.

Localization of Sop Proteins and Interaction of Plasmid DNA with the Cell Membrane of Host Bacteria in Partitioning

  • Kim, Sung-Uk;Nagai, Kazuo;Tamura, Gakuzo;Yu, Ju-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.3 no.4
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    • pp.261-265
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    • 1993
  • A sopA protein (41K) encoded by plasmid pXX288 was observed in the cytoplasm, whereas a sopB protein (37K) encoded by plasmid pXX157 was observed in the membrane fraction. Most of the sopB protein was solubilized from the crude membrane by treatment with Sarkosyl, which suggested that the protein may be located in the inner membrane. The sopA protein was precipitated at the concentration of 30 to 60% ammonium sulfate. The sedimentation profile of the crude membrane fraction showed a little difference according to culture media used, and the sopB protein existed in all fractions of inner membrane. The DNA of plasmids, pXX157, pXX300, and pXX167 co-sedimented with inner membrane fraction.

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Development Risk Management Platform Technology based on Intelligent SOP (지능형 SOP 기반 위기관리 플랫폼 기술 개발)

  • Kim, Ok-Ju;Lee, Chang-Yeol
    • Proceedings of the Korean Society of Disaster Information Conference
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    • 2016.11a
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    • pp.435-438
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    • 2016
  • 재난에 대비하여 각 기관에서는 자체의 위기관리 매뉴얼을 보유하고 있고, 이를 기반으로 재난대응 훈련 등을 실시하고 있다. 그러나 이러한 매뉴얼은 개념적이어서 재난 발생 시 현장에서 즉각적으로 수행하기에는 구체성이 부족하다. 본 연구는 이러한 관점에서 위기관리 매뉴얼을 SOP 기반의 시스템으로 변환하여 운영할 수 있는 플랫폼 기술을 개발하는데 있다. 플랫폼은 단위 행동 모임인 SOP 관리 모듈, 위기관리 매뉴얼 기반 재난 대응 절차를 수행을 위한 시나리오 관리 모듈, 그리고 기관의 담당자가 직접 해당 SOP를 수행하기 위한 정보인 조직도 관리 모듈로 구성되어 있으며, 실제 재난 대응을 통하여 수행한 활동에 대한 정보를 기록하여 빅 데이터를 분석하여 보완할 수 있는 기반을 마련하였다.

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