• Title/Summary/Keyword: SOD activity

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Reinforcement of Antioxidative Potentials by Korean Traditional Prescriptions on Mouse Plasma and Liver (전통 한약 탕제 투여에 의한 혈장 및 간 조직의 항산화력 증강 효과)

  • Hong, Seong-Gil;Lee, Mi-Young;Yoon, Yoo-Sik;Kang, Bong-Joo;Kim, Dae-Won;Cho, Dong-Wuk
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1661-1666
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    • 1999
  • Yungmijihwgang-Won, Yollyunggobon-Dan and Palmi-Hwan, Korea traditional prescriptions composed of oriental medical herbs, have been used successfully to improve human health and regimen. This study was designed to examine the mechanism of healthful effects of the Korea traditional prescriptions through its antioxidative potentials. Using in vitro antioxidative activity assay system such as DPPH radical quenching assay, superoxide anion radical scavenging assay and inhibition of TBARS production, three Korea traditional prescriptions were observed to have nearly the same antioxidative potentials as ascorbic acid, a well-known strong water-soluble antioxidant. Moreover, we observed reinforced antioxidative effects of these drugs in liver from mouse fed these drugs with 4 weeks. When liver homogenate was incubated with 2.2'-azobis(amidinopropane) dihydrochloride(AAPH), as a free radical initiator, we observed that oxidative damages were decreased and antioxidative potentials were increased in liver homogenate treated these drugs. However, enzymatic antioxidative system as superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase was not affected by drug administration.

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Isolation and Identification of Antioxidant Compounds of Various Solvents Extracted from Eriobotrya japonica Leaves (비파 잎의 추출용매별 항산화성 검증과 활성물질의 분리 및 동정)

  • Ham, Hyeon-Suk;Lee, Se-Yeul;Lee, Dong-Wan;Seong, Jong-Hwan;Kim, Han-Soo;Kim, Dong-Seob;Lee, Young-Guen
    • Journal of Life Science
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    • v.22 no.9
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    • pp.1166-1172
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    • 2012
  • To investigate potential medicinal or functional uses of Eriobotrya japonica, this study focused on the isolation and identification of antioxidant compounds from Eriobotrya japonica leaves. Various solvents were extracted from the leaves, and their scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals was measured, in addition to their superoxide dismutase-like activity, polyphenol compounds, and flavonoid content. Ethyl acetate extract exhibited the strongest scavenging effect in a 0.2 mM solution of DPPH ($63.24{\pm}2.20%$, $81.83{\pm}2.10%$, and $93.15{\pm}2.31%$ in 0.3, 0.7, and 1.0 mg/ml sample concentrations, respectively). The antioxidant effect of the ethyl acetate extract and methanol extract were generally stronger than that of n-hexane extract. The extracts were further purified by repeated silica gel column chromatography. The antioxidant compounds were identified as phytol, ${\beta}$-sitosterol, and (-)-loliolide using GC/MS.

Effect of Haedokjeongki-tang in Rat Liver after Monocrotaline Exposure (Monocrotaline으로 유발된 흰쥐의 간독성에 대한 해독정기탕의 효과)

  • Park, Hyun-Jeong;Kim, Jeong-Sang
    • Applied Microscopy
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    • v.37 no.1
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    • pp.1-10
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    • 2007
  • Monocrotaline (MCT) is a pyrrolizidine alkaloid (PA) plant toxin that produces hapatotoxicity in humans and animals. To felt the hypothesis, we investigated the possible protective effects of Haedokjeongki-tang as an antioxidant against MCT-induced liver injury in rats. Cells with apoptotic morphology have been observed in the livers of animals exposed to Ph and Haedokjeongki-tang. Whether apoptosis occurs in the livers of MCT-treated animals and whether it is required for full manifestation of pathological changes is not known, To determine this, rats were treated with 100 mg MCT/kg, and apoptosis was detected by transmission electron microscopy and TUNEL assay. MCT produced apoptosis in the liver by 6 h after treatment and increased by 24 h. Administration of Haedokjeongki-tang did affect liver structure and inhibit apopotosis in MCT-induced liver injury. Upon light and electron microscopic examination, we observed that Haedokjeongki-tang improved the morphological and histopathological changes of the liver caused by MCT-induced injury. MCT caused a time-dependent release of GOT and GPT, a marker of liver injury. Furthermore, we observed with respect to antioxidants status, catalase and superoxide dismutase activity tended to be higher in the MCT-treated rats than in the Haedokjeongki-tang administered rats. Our finding showed that Haedokjeongki-tang administration partially reduced liver injury after MCT exposure.

Antioxidant Effects and Improvement of Lipid Metabolism of Mulberry fruit, Mulberry Leaves and Silkworm Powder with Different Mixing Ratios in Streptozotocin-Induced Diabetic Rats (오디, 뽕잎 및 누에의 혼합비율에 따른 Streptozotocin 유발 당뇨쥐에서의 항산화 효과 및 지질대사개선 효과)

  • Kwon Eun-Hye;Jung Myung-Ae;Rhee Soon-Jae;Choi Sang-Won;Cho Sung-Hee
    • Journal of Nutrition and Health
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    • v.39 no.2
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    • pp.91-99
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    • 2006
  • This study was conducted to investigate the effects of mulberry fruit, mulberry leaves and silkworm powder with different mixing ratios on hepatic antioxidative system and lipid metabolism in streptozotocin-induced diabetic rats. Sprague-Dawley male rats weighing $100{\pm}10g$ were induced diabetic by 50 mg/kg bw streptozotocin and randomly assigned to following experimental groups; normal diet group (DM), 0.3% and 0.6% mulberry fruit diet groups (F and 2F), 0.3% mulberry leaves diet group (M), 0.3% silkworm powder diet group (S), 0.15% mulberry fruit+0.15% mulberry leaves diet group (FM), 0.15% mulberry fruit+0.15% silkworm powder diet group (FS), 0.1 % mulberry fruit+0.1 % mulberry leaves+0.1% silkworm powder diet group (FMS). The experimental diets were fed for 4 weeks. Hepatic SOD activity was not changed significantly by any of single or combined supplementations of mulberry fruit, leaves and silkworm powder but GSH-px and catalase activities were increased by the groups supplemented with two or three of the test ingredients (FM, FS, FMS) as compared with the DM group. Hepatic TBARS value was not reduced significantly by any of the supplementations but lipofuscin contents were significantly reduced in the FM, FS and FMS groups as compared with the DM group. Hepatic mitochondria and microsomal carbonyl values were reduced by the single and combined supplementations of the test ingredients. Hepatic HMG-CoA reductase activities were increased in the all supplementation groups as compared with the DM group. Hepatic total lipid and triglyceride contents were increased but cholesterol contents reduced in the supplemented groups. The effects on the enzyme activities, peroxide or its products and lipid contents were most remarkable in the FMS group. In conclusion, mulberry fruit, mulberry leaves and silkworm powder have the favorable effects on antioxidative system and lipid metabolism in the diabetic liver and the mulberry fruit, leaves and silkworm powder with equal ratio exert the synergistic effect expectedly to prevent diabetic complications.

Influence of Dietary n3 Polyunsaturated Fatty Acids on Plasma Lipid-Lowering Effect and Peroxidation Level In Rats (쥐에서 n3계 불포화지방산 식이의 혈장지질 저하효과와 과산화물형성에 미치는 영향)

  • Choi, Joo Sun
    • Journal of Nutrition and Health
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    • v.23 no.6
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    • pp.408-417
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    • 1990
  • To compare the hypolipidemic effect of n6 linoleic acid n3 $\alpha$-linolenic acid and n3 eicosapentaenoic acid plus docosahexaenoic acid, male Sprague Dawley rats weighing about 450g were fed the experimental diets for 6 weeks which composed of fat at 15%(W/W) level and were different only in dietary PUFA. Dietary fat was corn oil, perilla oil, and fish oil concentrate as a source of n6 linoleic acid, n3 $\alpha$-linolenic acid, and n3 eicosapentaenoic acid+docosahexaenoic acid, respectively. Plasma total Chol and HDL-chol levels were significantly-lower in fish oil group than in corn oil and erilla oil groups. Plasma cholesterol lowering effect of PUFa was in the order of n3 EPA+DHA>n3 $\alpha$-linolenic acid>n6 linoleic acid. Plasma TG was significantly lower in both fish oil and perilla oil groups than in corn oil group. Plasma TG-lowering effect was greater by n3 PUFA (EPA+DHA, $\alpha$-linolenic acid) than by n6 PUFA(linoleic acid). However, there were no significant effects on lipoprotein pattern hemolysis, and the levels of tocopherol and malondialdehyde in plasma and RBC by difference dietary fat with sufficient tocopherol supplement. Liver superoxide dismutase activity was significantly increased in proportion to the degree of fat unsaturation, thereby resulted in the lower level of MDA in fish oil group. In conclusion, fish oil and perilla oil rich in n3 PUFA may have important nutritional applications in the prevention and treatment of atherosclerotic disease.

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Changes of antioxidant enzyme activities subjected to water stress in soybean leaves (대두(大豆)에서 분석(水分)스트레스에 의(依)한 항산화효소(抗酸化酵素)의 활성도(活性度) 변화(變化))

  • Kim, Tae-Sung;Kang, Sang-Jae;Park, Woo-Churl
    • Current Research on Agriculture and Life Sciences
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    • v.16
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    • pp.24-30
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    • 1998
  • This experiment was carried out to elucidate and study about plant defense mechanism subjected to water stress(drought, flooding). We measured water content, total soluble protein content as stress marker and superoxide dismutase(SOD), catalase (CAT) as antioxidant enzymes subjected to water stress(drought, flooding) and recovery in soybean leaves. The results obtained were as follows; Two soybean lines(keonolkong, euhakong)leaves exposed to water stress (drought, flooding) showed premature senescence as evidence by the decrease in water content, and total soluble protein content, but those of soybean leaves subject to water stress recovery for 3 days were recovered. Visual damage was much worse at drought stress than flooding stress and was worse keunolkong than enhakong. The activity of superoxide dismutase, catalase subjected to water stress(drought, flooding)was on the decrease, but degree of decrease was different from a sort of soybean lines, drought and flooding stress.

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Antioxidative Effects of Mugwort (Artemisia vulgaris L.) Extracts Diet on ICR Mouse Skin (애엽 추출물 섭취가 ICR 마우스의 피부조직에 미치는 항산화 효과)

  • Park, Si-Hyang;Cho, Duck-Moon;Choi, Gyeong-Lim;Choi, Yeung-Joon;Choi, Jin-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.12
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    • pp.1523-1528
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    • 2007
  • The feeding effects of mugwort (Artemisia vulgaris L.) extracts (ME) on the anti-oxidative actions of ICR mouse skin was investigated. To study the antioxidative effects of ME on ICR mouse skin, female ICR mice were grouped into basic diet group (control), ascorbic acid diet group (AA-2.5, AA-5.0, AA-10.0 and AA-20.0 mg/kg BW/day) as a positive control and experimental diet group (mugwort extract; ME-25, ME-50, ME-100, and ME-200 mg/kg BW/day) and fed for 10 weeks. Protein contents in ME-50, ME-100, and ME-200 feeding group were increased ($3.1%{\sim}11.1%$) and hydroxyl radical contents were significantly decreased ($10.4%{\sim}17.4%$) compared to control group. Oxidative stress signals and oxidized protein contents were significantly reduced to the range of 15.3 to 17.1% in ME-100 and ME-200 groups. Also, superoxide dismutase (SOD) activity was significantly increased to the range of 15.0% to 23.3% in ME-100 and ME-200 groups. Catalase activities were significantly increased ($14.0%{\sim}36.9%$) in all groups in a dose-dependent pattern. Antioxidative ability of ME showed similarity to that of ascorbic acid.

The Antioxidant and Skin-whitening Effects of Saccharomyces cerevisiae FT4-4 Isolated from Berries Grown in Sunchang (화장품 소재로서 순창 베리류 유래 Sacchromyces cerevisiae FT4-4의 항산화 활성 및 미백 효과)

  • Seo, Ji won;Ryu, Myeong Seon;Yang, Hee-Jong;Jeong, Su-Ji;Jeong, Do-Youn
    • Journal of Life Science
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    • v.31 no.2
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    • pp.175-182
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    • 2021
  • Saccharomyces lysate has the well-known function of soothing the skin in various ways: it is an anti-irritant and can treat skin care conditions, such as skin whitening and antioxidative activity. However, data on the safety for use of Saccharomyces lysate in cosmetics and skin care products are still limited. To design a new cosmetic material with antioxidant and skin-whitening effects, 80 yeast strains were isolated from berries grown in Sunchang. Among the isolates, the FT4-4 strain, which exhibited superior biological activities, was selected for further experiments. The FT4-4 strain was identified as Saccharomyces cerevisiae by 18S rRNA gene sequencing analysis. S. cerevisiae FT4-4 showed higher DPPH radical-scavenging (51.41%), superoxide dismutase (62.23%), and tyrosinase inhibition (64.75%) activities. The highest yield of biomass (3.16 g/l) and maximum growth rate of S. cerevisiae FT4-4 were observed within 16 h. Furthermore, the cytotoxicity potential of S. cerevisiae FT4-4 on B16F10 melanoma cells was measured by an MTT assay, and the results indicated that S. cerevisiae FT4-4 had a capacity to inhibit melanin up to 72.02% at an initial 10 mg/ml concentration. These results suggest that S. cerevisiae FT4-4 could be a promising candidate as a multi-functional material for application in the cosmetic industry, especially because of its antioxidant and skin-whitening effects.

Comparison of antioxidant activities of pearled and wholegrain barley harvested in Jeju (도정 유무에 따른 제주산 보리의 항산화 활성 비교)

  • Kang, Yuri;Kim, Hyun Jung
    • Korean Journal of Food Science and Technology
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    • v.53 no.3
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    • pp.252-259
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    • 2021
  • Barley contains health-beneficial compounds, such as phenolic acids, flavonoids, lignans, and tocopherols. The antioxidant activities of pearled and wholegrain hull-less, premature, and black barley harvested in Jeju were investigated by measuring total phenolic content (TPC), total flavonoid content (TFC), phenolic compounds, tocopherols (T), and tocotrienols (T3), along with their in vitro antioxidant activities. Consequently, TPC and TFC in wholegrain barley groups were higher than those in pearled barley. Gallic acid (1.55-2.98 ㎍/g) and protocatechuic acid (0.67-2.84 ㎍/g) were the predominant phenolic compounds in barley. Total T and T3 concentrations of wholegrain barley groups were significantly higher than those of pearled barley (p<0.05). Except for the metal chelating effect and reducing power, the in vitro antioxidant activities of wholegrain barley were significantly higher than those of pearled barley. These results indicate that wholegrain Jeju barley can be used as a natural antioxidant source in the food industry.

Studies on Antioxidant and Anti-hyperlipidemic Effects in Induced Hypercholesterolemic Rats of Steamed Achyranthis Bidentatae Radix (우슬(牛膝)의 증숙(蒸熟)에 따른 항산화 효능 및 고지혈증 유발 흰쥐에 대한 고지혈증 개선 효능에 대한 연구)

  • Kim, Jeong-Soo;Park, MyungJae;Kim, SuJi;Shin, Mi-Rae;Lee, AhReum;Park, Hae-Jin;Roh, Seong-Soo;Seo, Young Bae
    • The Korea Journal of Herbology
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    • v.33 no.6
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    • pp.43-60
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    • 2018
  • Objectives : This study was conducted to compare the effect of Achyranthis Bidentatae Radix on antioxidant changes and antihyperlipidemics in hypercholesterolemic rats according to changes in the number of steams. Methods : The experimental animals were divided into six groups: normal (Nor), high cholesterol diet (Veh), high cholesterol diet with Simvastatin 5 mg/kg (Sim), high cholesterol diet with AR0 extract 200 mg/kg (AR0), high cholesterol diet with AR6 extract 200 mg/kg (AR6) and high cholesterol diet plus AR9 extract 200 mg/kg (AR9). I measured HDL, LDL TC, TG contents and ROS from each serums and compared them. I analyzed antioxidant-related protein expressions and cholesterol-related protein expressions in each liver tissues. I also performed optical microscopic analysis through liver tissue staining. Results : The Antioxidant activity of Achyranthis Bidentatae Radix was increased by steaming processes. In vivo, AR0 decreased liver weight in hyperlipidemic rats. LDL TC, TG, AI and CRF were decreased but HDL was increased in AR0. AST, ALT, creatinine, and BUN were decreased in AR0. ROS was decreased in AR0, AR6, and AR9. SOD, catalase and GPx were decreased by steaming processes. p-AMPK was decreased but SREBP-2, p-ACC, and HMGCR were increased by steaming processes. Histologic analysis showed that AR0 decreased liver fat accumulation. Conclusion : In conclusion, AR0 can be developed as a treatment for hyperlipemia. And it is required to research on antioxidant effect changes among different times steamed Achyranthis Bidentatae Radix.