• Bulletin of the Korean Chemical Society
• /
• v.35 no.9
• /
• pp.2753-2757
• /
• 2014

INtegrase Interactor 1 protein (INI1/hSNF5) or BRG1-associated factor 47 (BAF47) is a SWI/SNF-related matrix associated actin dependent regulator of chromatin subfamily B member. DNA binding domain of INI1/hSNF5 is cloned into E.coli expression vectors, pET32a and purified as a monomer using size exclusion chromatography. NMR data show that $INI1^{DBD}$ has folded state with high population of ${\alpha}$-helices. By fluorescence-quenching experiments, binding affinities between $INI1^{DBD}$ and two double stranded DNA fragments were determined as $29.9{\pm}2.6{\mu}M$ (GAL4_1) and $258.7{\pm}5.8$ (GAL4_2) ${\mu}M$, respectively. Our data revealed that DNA binding domain of INI1/hSNF5 binds to transcriptional DNA sequences, and it could play an important role as a transcriptional regulator.

• Journal of the Korean Ceramic Society
• /
• v.55 no.2
• /
• pp.126-134
• /
• 2018

Using aqueous solution free radical polymerization with glacial acrylic acid (GAA), maleic anhydride (MA) and sodium methallyl disulfonate (SMADS), a novel linear polycarboxylate dispersant was synthesized for ceramics. Dispersant linear structural characterization was done by FTIR, $^1H$ NMR, HPLC and GPC, and the ratio of monomers was determined using an orthogonal experiment. This research is focused on the effects of polymerization temperature, monomer mole ratios and dosage of initiator on ceramic slurry viscosity with linear polycarboxylate dispersant for ceramic dosage rate of 0.30% (based on dry slurry), all of which were investigated by single factor test. The best polymerization conditions for linear GAA-MA-SMADS are when n(AA) : n(MA) : n(SMADS) equals 3.0 : 1.0 : 0.5, the molecular weight of the polymer is 4600 daltons, the initiator sodium persulfate accounts for 7% of the total mass of polymerized monomers, the polymerization temperature is $90^{\circ}C$ and the reaction time is 2 h. The ceramic body slurry viscosity drops from $820mPa{\cdot}s$ to $46mPa{\cdot}s$ when the concentration of the polycarboxylate dispersant is 0.30%.

• International Journal of Control, Automation, and Systems
• /
• v.6 no.3
• /
• pp.413-423
• /
• 2008

The template matching method is used as a simple method to track objects or patterns that we want to search for in the input image data from image sensors. It recognizes a segment with the highest correlation as a target. The concept of this method is similar to that of SNF (Strongest Neighbor Filter) that regards the measurement with the highest signal intensity as target-originated among other measurements. The SNF assumes that the strongest neighbor (SN) measurement in the validation gate originates from the target of interest and the SNF utilizes the SN in the update step of a standard Kalman filter (SKF). The SNF is widely used along with the nearest neighbor filter (NNF), due to computational simplicity in spite of its inconsistency of handling the SN as if it is the true target. Probabilistic Strongest Neighbor Filter for m validated measurements (PSNF-m) accounts for the probability that the SN in the validation gate originates from the target while the SNF assumes at any time that the SN measurement is target-originated. It is known that the PSNF-m is superior to the SNF in performance at a cost of increased computational load. In this paper, we suggest an image tracking algorithm that combines the template matching and the PSNF-m to estimate the states of a tracked target. Computer simulation results are included to demonstrate the performance of the proposed algorithm in comparison with other algorithms.

• Journal of Nuclear Fuel Cycle and Waste Technology
• /
• v.1 no.1
• /
• pp.1-7
• /
• 2013

The safe management of radioactive waste is a national task required for sustainable generation of nuclear power and for energy self-reliance in Korea. Since the initial introduction of nuclear power to Korea in 1978, rapid growth in nuclear power has been achieved. This large nuclear power generation program has produced a significant amount of radioactive waste, both low- and intermediate-level waste (LILW) and spent nuclear fuel (SNF); and the amount of waste is steadily growing. For the management of LILW, the Wolsong LILW Disposal Center, which has a final waste disposal capacity of 800,000 drums, is under construction, and is expected to be completed by June 2014. Korean policy about how to manage the SNF has not yet been decided. In 2004, the Atomic Energy Commission decided that a national policy for SNF management should be established considering both technological development and public consensus. Currently, SNF is being stored at reactor sites under the responsibility of plant operator. The at-reactor SNF storage capacity will run out starting in 2024. In this paper, the fundamental principles and steps for implementation of a Korean policy for national radioactive waste management are introduced. Korean practices and prospects regarding radioactive waste management are also summarized, with a focus on strategy for policy-making on SNF management.

• Nuclear Engineering and Technology
• /
• v.50 no.3
• /
• pp.504-511
• /
• 2018

In Lithuania, all spent nuclear fuel (SNF) resulted from the operation of the Ignalina Nuclear Power Plant (INPP), which had two Russian Acronym for "Channelized Large Power Reactor"-type reactors. After the final shutdown, the total amount of SNF at the INPP was approximately 22,000 fuel assemblies. All these assemblies will be stored for about 50 years and disposed of after that. The decision to shut down and decommission both reactors in Lithuania before termination of design period raises a significant challenge for the treatment of accumulated SNF. Therefore, various techniques and technologies for SNF management were developed and justified for that specific case, and a set of special equipment was installed at the INPP, the effectiveness of which was demonstrated during its operation. This article presents unique techniques related to the management of SNF adopted and commissioned at the INPP after its operation shutdown, namely fuel rod cladding leak tightness control system and special equipment for collection of possible spillage during handling of SNF assembly in the hot cell. The operational experience and measurement results of fuel rod cladding leak tightness control system are presented.

• Nuclear Engineering and Technology
• /
• v.53 no.11
• /
• pp.3704-3710
• /
• 2021

The characteristics of the external radiation on the surface of the casks for spent nuclear fuel (SNF) storage by dry method are investigated for the case when the spatial distribution of SNF in the basket changes due to the destruction of the fuel rod claddings. The surface areas are determined, where the changes in fluxes of neutrons, produced by ²⁴⁴Cm actinide, and γ-quanta, produced by long-lived isotopes, are maximum in the result of the decrease in the height of the SNF area. Concrete (VSC-24) and metal (SC-21) casks are considered as examples. The procedure of periodic measurement of the dose rate of neutrons or γ-quanta at the specified points of the cask surface is proposed for identifying the fuel rod cladding destruction. Under normal operation, the decrease in the dose rate produced by neutrons as the function of SNF storage duration is determined by the half-life of ²⁴⁴Cm, and for γ-quanta - by the half-lives of long-lived SNF isotopes. Consequently, a stepwise change in the dose rate of neutrons or γ-quanta, detected by the measurements, as compared to the previous one, would indicate the destruction of the fuel rod claddings.

• Nuclear Engineering and Technology
• /
• v.54 no.10
• /
• pp.3766-3777
• /
• 2022

It is essential to ensure the safety of spent nuclear fuel (SNF) transport cask in drop situation that is included in transport accident scenarios. The safety of the drop situation is affected by the impact absorption performance of impact limiters. Therefore, when designing an impact limiter, the uncertainty in the material properties that affect the impact absorption performance must be considered. In this study, the material properties of the wood inside the impact limiter were selected as the variables for a parametric study. The sensitivity analysis of the drop response of the SNF transport cask with impact limiter was performed. The minimum wood strength required to prevent a direct collision between the cask and floor was derived from the analysis results. In addition, the plastic strain response was analyzed and strain-based evaluation was performed. Based on this result, the critical values of wood properties that change the impact dynamic characteristics were investigated. Finally, the optimal material properties of wood were obtained to secure the structural safety of the SNF transport cask. The results of this study can contribute to the development of SNF transport cask, thereby ensuring safety in transport accident conditions.

• Proceedings of the Korean Radioactive Waste Society Conference
• /
• 2004.06a
• /
• pp.261-267
• /
• 2004

Inventories, projections, and characteristics of spent nuclear fuel(SNF) generated from domestic nuclear power plants were updated to support high-level waste disposal system design. The historical and projected inventory by the end 2055 is expected to be 20,500 and 14,800MTU for PWR and CANDU spent nuclear fuel, respectively The ratio of quantity for TEX>$17{\times}17$ SNF was shown to be 0.6 as of 2003. The amount of TEX>$17{\times}17$ SNF, however, will be less than that of TEX>$16{\times}16$ KSFA after 2012, while the quantity of TEX>$16{\times}16$ KSFA will reach to 70% of the total spent fuels in the 2055. Average turnup of SNF revealed ~36GWD/MTU and ~40GWD/MTU for the period of 1994-1999 and 2000-2003, respectively. It is expected that the average burnup of SNF will exceed 45GWD/MTU at the end of 2000's. Therefore, it seems reasonable to use the TEX>$17{\times}17$ 4.5w/o, 45GWD/MTU as the Reference SNF at present state. The TEX>$16{\times}16$ KSFA 4.5w/o, 55GWD/MTU, however, should be Reference SNF after ~2010.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.128-128
• /
• 2018

Nypa fruticans wurmb (NF) has been used as traditional medicinal food in Asian countries. Especially, NF has been used for conventional medicine to treat inflammatory periodontal diseases. Previous studies have been shown that NF has large amount of useful constituents such as phenolic acids, polyphenols and flavonoids. Also, NF is known as having medicinal effects such as anti-oxidant, anti-inflammatory and cholesterol-lowering effects. NF has recently been attracted to use complementary medicinal food on inflammatory diseases in Korea. However, there are no obvious effects in inflammatory and metabolic diseases also mechanisms has been studied yet. The purpose of this study was to investigate the anti-inflammatory effects of NF and steamed-NF (SNF), which recently has been used as health food, using Human keratinocyte cell line (HaCaT) and Human mast cell line (HMC-1). The cytotoxicities of NF and SNF were measured by using MTT assays in HaCaT cells and HMC-1 cell. To evaluate anti-inflammatory effects of NF and SNF, HaCaT cells were stimulated with tumor necrosis factor $(TNF)-{\alpha}$ and Interferon $(IFN)-{\gamma}$. Also, HMC-1 cells were stimulated with phorbol-12-myristate-13-acetate (PMA) and A23187 calcium ionophore (A23187) to induce allergic inflammation. Inflammatory cytokine were measured by enzyme-linked immunosorbent assay (ELISA). In this result, the extract of NF and SNF (0.01 - 1mg/ml) did not show cytotoxicity in HaCaT cells and HMC-1 cells. In addition, the NF and SNF suppressed the production of interleukin (IL)-6 and IL-8 in HaCaT cells at highest concentration. Furthermore, the treatment of SNF significantly inhibited the secretion level of IL-8 in PMA plus A23187-stimulated HMC-1 cells compared with NF treatment group. These results suggest that the extract of NF and SNF may serve as a potential therapy for skin inflammatory diseases.

• Journal of Life Science
• /
• v.10 no.1
• /
• pp.40-44
• /
• 2000

The SNF2/SW ATPase/helicase family comprises proteins form a variety of species with in vivo functions, such as transcriptional regulation, maintenance of chromosome stability during mitosis, and various types of DNA repair. Here, we reported the characterization of h게2+gene which was iolated by PCR amplification using the conserved domain of SNF2 motifs. Sequence analysis of PCR product showed striking evolutionary conservation among the SNF2 family of proteins. Two transcripts of 6.7 and 3.4 Lb were detected by Northern blot analysis. furthermore, the intensities of these two bands were increased by ultraviolet(UV) irradiation. These results indicate that the hrp2+ is a novel member of the SNF2 family of proteins and is one of the UV-inducible genes in S. pombe. To determine the level of transcripts of hrp2+ gene during cellular growth, Northern blot analysis were performed. This result indicates that the level of hrp2+transcript reached its maximum before cells entered the exponential growth phase. This suggests that hrp2+ gene is experssed mainly at the early stage of cell growth.