• Archives of Pharmacal Research
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• 제17권5호
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• pp.348-353
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• 1994

The activity fractionation upon the MeOH extract of the root of trichosanthes kirilowii led to the isolation of eight cucurbitane tritepense namely cucurbitacin .betha. (I), isocucurbitacin .betha.(II), cucurbitacin D(III), isocucurbitacin D(IV), 3-epi-isocucurbitacin .betha(V), dihydrocucurbitain .betha. (VI), dihydroisocucurgbitachin .betha. (VII) and dihydrocucurbitacin E (VIII), as active principles. All isolates were shown to exhibit significant cytotoxicity against cultured human tumor cells, including A-549, Sk-OV-3, Sk-MEL-2, XF-498 and HCT 15, with an exceptionally high potency.

• 생약학회지
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• 제26권1호
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• pp.47-50
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• 1995

EtOAc soluble part of MeOH extract of Anemarrhena asphodeloides rhizome was evaluated for the cytotoxicity against the five kinds of human tumor cell lines (A-549, SK-OV-3, SK-MEL-2, XF498 and HCT15) in vitro. Bioassay-guided fractionation of EtOAc soluble part led to the isolation of active compound which was identified as timosaponin A-III showed potent cytotoxic activity, but its genin, sarsasapogenin, did not show cell growth inhibition.

• Archives of Pharmacal Research
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• 제18권3호
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• pp.164-168
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• 1995

In order to elucidate the cytotoxicity-structure correlation of ginseng-derived components, several prosapogenins and sapogenins were prepared from Korean red ginseng (Panax ginseng) saponins by acid hydrolysis or alkaline cleveage, and their chemical structures were identified by a combination of spectral and physical methods. Some of these degradation products showed the cytotoxic activities against various cancer cell lines, A549, SK-OV-3, SK-Mel-2, P388, L1210 and K562. The significant difference in cytotoxicity between stereoisomers was not found and the activity was inversely proportional to the number of sugars linked to sapogenins. Diol-type prosapogenins and sapogenins showed higher cytotoxicity than triol-type ones.

• 생약학회지
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• 제31권4호
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• pp.401-406
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• 2000

Marine sponges are known to be a source of diverse sterols. In our study on the cytotoxic components of the marine sponge Spirastrella abata, $5{\alpha},8{\alpha}-epidioxy\;{\Delta}^6$ sterols (1-5) and $5{\alpha},8{\alpha}-epidioxy\;{\Delta}^{6,9(11)}$ sterols (6-7) were isolated. The structures were identified based on the analyses of $^1H-NMR,\;^{13)C-NMR$, and MS data. These compounds were assayed for cytotoxicity against 5 human solid tumor cell lines including A549, SK-OV- 3, SK-MEL-2, XF498, and HCT15.

• 약학회지
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• 제41권4호
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• pp.524-529
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• 1997

Seventy three hydrolysable tannins and related compounds were isolated from seven Euphorbia plants. Among them, 28 compounds including nine gallotannins, eleven ellagitannins and eight related compounds were selected according to the structural similarity. Cytotoxicity of them on the human tumor cell lines including A-549, SK-OV-3, SK-MEL-2, XF-498 and HCT-15 were evaluated by the SRB method in vitro. 3,4,6-Tri-O-galloyl-D-glucose was shown to exhibit most potent cytotoxic effect($4.4{\mu}g/ml).

• Applied Biological Chemistry
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• 제41권2호
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• pp.187-190
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• 1998

동결건조(凍結乾燥)한 누에 유래(4령유충(齡幼蟲), 암 수 번데기, 암 수 성충(成蟲)) 및 건조 뽕나무 유래 재료(잎, 오디, 상백피(桑白皮))의 메탄을 추출물, 백강잠(白彊蠶) 및 누에 4령유충(齡幼蟲) 잠분(蠶糞)의 메탄올 추출물의 5종 인간(人間) 암세포주(癌細胞株)(A549 lung, SK-OV-2 ovarian, SK-MEL-2 melanoma, XF-498 CNS, HCT-15 colon tumor cell lines)에 대한 세포독성(細胞毒性)을 sulforhodamine B법을 이용하여 in vitro 검정하였다. 공시시료중 잠분(蠶糞)의 70% 메탄올 열탕추출물(熱湯抽出物)은 이들 암세포주(癌細胞株)에 대하여 강한 세포독성(細胞毒性)을 나타내었으나, 잠분(蠶糞)의 메탄올 추출물 및 오디와 상백피(桑白皮)의 메탄을 추출물은 중간 정도의 활성을 보였다. 기타 물질들은 이들 암세포주(癌細胞株)에 거의 독성을 보이지 않았다. 70% 메탄올 열탕추출물(熱湯抽出物)이 강한 세포독성(細胞毒性)을 나타내어, 용매 분획한 결과 클로로포름과 에틸아세테이트 획분이 암세포주(癌細胞株)에 대하여 가장 강한 세포독성(細胞毒性)을 보였다. 결론적으로, 잠분(蠶糞), 상백피(桑白皮) 및 오디의 항암활성(抗癌活性)은 이들의 약리작용의 일부를 설명할 수 있을 것으로 생각된다.

• Advances in nano research
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• 제7권4호
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• pp.233-240
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• 2019

The present research work reports in-vitro anti-cancer activity of biologically synthesized ZnO nanoparticles (ZnO NPs) against human carcinoma cells viz SCC-40, SK-MEL-2 and SCC-29B using Sulforhodamine-B (SRB) Assay. ZnO NPs were synthesized by a unique and novel biological route using Temperature-gradient phenomenon where the extract of combination of Catharanthus roseus (L.) G. Don (C. roseus), Azadirachta indica (A. indica), Ficus religiosa (F. religiosa) and NaOH solution were used as synthesis medium. The morphology of the ZnO NPs was characterized by Transmission Electron Microscopy (TEM). TEM images reveal that particle size of the samples reduces from 76 nm to 53 nm with the increase in reaction temperature and 68 nm to 38 nm with the increase in molar concentration of NaOH respectively. XRD study confirms the presence of elements and reduction in crystallite size with increase in reaction temperature and NaOH concentration. The diffraction peaks show broadening and a slight shift towards lower Bragg angle ($2{\theta}$) which represents the reduction in crystallite size as well as presence of uniform strain. The FTIR spectra of the extract show transmittance peak fingerprint of Zn-O bond and presence of bioactive molecules These NPs exhibit inhibition greater than 50% for SCC-40, SK-MEL-2 and SCC-29B cell lines and more than 50% cell kill for SCC-29B cells at concentrations < $80{\mu}g/ml$. Nanoparticles with smallest size have shown better anti-cancer activity and peculiar cell-selectivity. The combination of extracts of these plants with ZnO NPs can be used in targeted drug delivery as an effective anti-cancer agent, a potential application in cancer treatment.

• 한국응용과학기술학회지
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• 제24권2호
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• pp.109-116
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• 2007

2-Chlorobenzoyl hydrazine refluxed with benzoyl isothiocyanate and phenyl isothiocyanate in ethanol for 3 hours to give benzamide derivative (1) and anilinederivative (2) on yield of 71%and 95%, respectively. Benzamide derivative (1) reacted with ethanolic sodium hydroxide on reflux to afford cyclization product (3), followed by general substitution reaction of two steps to give acetamide (5), and derivatived acetamides 7a-7k, while aniline derivative (2) reacted with ethanolic sodium hydroxide on reflux to afford another cyclization product (4). Thiol (4) reacted with N-phenyl chloroacetamide in the presence of potassim carbonate to give acetamide derivative (6). Compounds 1-7kwere evaluated for their growth inhibition against five cancer cell lines, including human lung carcinoma (A-549), human prostate cancer (DU145), human colon adenocarcinoma (HT-29), human malignant melanoma (SK-MEL-2) and human ovary malignant ascites (SK-OV-3) with sulforhodamine B (SRB) assay. All compounds (1-7k) showed low inhibition activities under 50% on 100M concentration.

• 생약학회지
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• 제43권1호
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• pp.6-9
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• 2012

The $CH_2Cl_2$ soluble part of the leaves of Helianthus tuberosus L. (Compositae) exhibited a potent cytotoxic activity against the cultured human tumor cell lines including A-549, SK-OV-3, SK-MEL-2, XF498 and HCT-15 in vitro. Bioassaydirected fractionation of the $CH_2Cl_2$ soluble part of this plant led to the isolation of four cytotoxic sesquiterpene lactones having ${\alpha}$-methylene-${\gamma}$-lactone ring in the molecule. On the basis of physical and spectral evidences, their structures were characterized as ${\Delta}^{4,15}$-isoatripliciolide tiglate (1), ${\Delta}^{4,15}$-isoatripliciolide methacrylate (2), budlein A isobutylate (3) and budlein A tiglate (4). The ${\Delta}^{4,15}$-isoatripliciolide tiglate (1) showed the most potent cytotoxic activity ($0.26{\mu}M<ED_{50}<2.16{\mu}M$) against all of the cell lines tested.

• Archives of Pharmacal Research
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• 제23권2호
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• pp.155-158
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• 2000

Bioassay-guided fractionation of Crataegus pinnatifida (Rosaceae) gave two cytotoxic ursane-type triterpenes which were identified as uvaol (1) and ursolic acid (2) by physicochemical and spectroscopic methods. 3-Oxo-ursolic acid (3) was synthesized from ursolic acid (2) by Jones method. The cytotoxic activities of these compounds were tested against murine L1210 and human cancer cell lines (A549, SK-OV-3, SK-MEL-2, XF498, and HCT15) in vitro. Compounds 1 and 2 showed moderate cytotoxicities against L1210, whereas they showed weak activities against human cancer cell lines. However compound 3 exhibited potent cytotoxic activities both in murine and in human cancer cell lines.