• Natural Product Sciences
• /
• 제19권2호
• /
• pp.155-159
• /
• 2013

Honokiol, a naturally occurring neolignan mainly found in Magnolia species, has exhibited a potential anti-proliferative activity in human cancer cells. However, the growth inhibitory activity against hepatocellular carcinoma cells and the underlying molecular mechanisms has been poorly determined. The present study was designed to examine the anti-proliferative effect of honokiol in SK-HEP-1 human hepatocellular cancer cells. Honokiol exerted anti-proliferative activity with cell-cycle arrest at the G0/G1 phase and sequential induction of apoptotic cell death. The cell-cycle arrest was well correlated with the down-regulation of checkpoint proteins including cyclin D1, cyclin A, cyclin E, CDK4, PCNA, retinoblastoma protein (Rb), and c-Myc. The increase of sub-G1 peak by the higher concentration of honokiol ($75{\mu}M$) was closely related to the induction of apoptosis, which was evidenced by decreased expression of Bcl-2, Bid, and caspase-9. Hohokiol was also found to attenuate the activation of signaling proteins in the Akt/mTOR and ERK pathways. These findings suggest that the anti-proliferative effect of honokiol was associated in part with the induction of cell-cycle arrest, apoptosis, and dow-nregulation of Akt/mTOR signaling pathways in human hepatocellular cancer cells.

• 한국자원식물학회지
• /
• 제22권4호
• /
• pp.312-316
• /
• 2009

In the present study, the anti-cancer activity of 80% ethanol extracts from 120 kinds of medicinal herbs and native plants were investigated. Among them, the barks of Melia azedarach L. var. japonica Makino showed the highest cytotoxicity in HCT-15 human colon cancer cell. With this result, we carried out hollow fiber (HF) assay and anti-metastasis study to confirm the anti-cancer effects of M. azedarach var. japonica. In MTT assay, M. azedarach var. japonica.inhibited the proliferation of HCT-15 cells in dose-dependent manner. HF assay was carried out using A549 human adenocarcinoma cell, HCT-15 and SK-Hep1 human liver cancer cell via intraperitoneal (IP) and subcutaneous (SC) site. As a results, SK-Hep1 implanted in IP site showed the highest cytotoxicity. The result from metastatic model using B16/BL6 mouse corresponded to that of HF assay. These results suggest that the ethanol extract from M. azedarach var. japonica. might have a potent anti-cancer activity and advanced study is needed for the development of novel natural anti-cancer drug.

• 생약학회지
• /
• 제43권4호
• /
• pp.291-296
• /
• 2012

The dried roots of Angelica decursiva and Peucedanum praeruptorum are registered as the official sources for the drug 'Junho' in Korean Herbal Pharmacopoeia. In this study the essential oils were extracted from the roots of the two plants by steam distillation. Their compositions and the biological activities were compared. As the results of GC and GC-MS analysis, forty one and thirty five compounds were identified in the essential oils of Angelica decursiva and Peucedanum praeruptorum, respectively. Both of the two essential oils contained ${\alpha}$-pinene as the most abundant component. They showed similar significant antioxidant activities in DPPH scavenging assay, and reducing power test. Their dose dependent inhibitions of the nitrosamine formation were identified in experiment using BHA as control. In MTT test of three cancer cell lines, HeLa, MCF-7 and SK-Hep-1, the essential oil from A. decursiva showed stronger activities than that of P. praeruptorum and their common main component, ${\alpha}$-pinene.

• 약학회지
• /
• 제55권5호
• /
• pp.398-403
• /
• 2011

Ligusticum chuanxiong (Umbelliferae) is a perennial herb that has been used for invigoration of blood in Korean traditional medicine. It is especially important in gynecological therapy of amenorrhea and dysmenorrhea. In this study, the essential oil of L. chuanxiong was obtained by steam distillation and its main components of L. chuanxiong, Z-ligustilide and butylidene phthalide, were isolated by silica gel column chromatography. We investigated the cytotoxic effects of the essential oil fraction of L. chuanxiong and its main components on MCF-7, HeLa and SK-Hep-1 cell lines by measuring the number of surviving cancer cells after treatment through direct cell counting and MTT analysis, and by examining the morphological changes under the microscope. The essential oil from the rhizomes of L. chuanxiong and its main components showed significant cytotoxic activities for all three tested cell lines. We also observed morphological changes of shrinking and blebbing in the membranes of the three cell lines, depending on the concentration of L. chaunxiong oil or its main components.

• Molecules and Cells
• /
• 제21권2호
• /
• pp.218-223
• /
• 2006

The effect of poly(ADP-ribosyl)ation on the stability of p53 in SK-HEP1 cells treated with UV light was examined. Intracellular levels of p53 increased in cells treated with a low dose of UV light ($20J/m^2$), whereas they increased but then declined after a higher dose of UV ($100J/m^2$). Intracellular levels of p53 in the UV treated SK-HEP1 cells were dependent on the UV dose. Use of proteasome inhibitors revealed that p53 is degraded by proteasomal proteolysis after high doses of UV light. We present evidence that, at low doses, poly(ADP-ribose)polymerase (PARP) poly(ADP-ribosyl) ates p53 and protects it from proteasomal degradation before caspase-3 is activated, whereas at high doses the cells undergo UV induced apoptosis and PARP is cleaved by caspase-3 before it can protect p53 from degradation. Destabilization of p53 by cleavage of PARP may be important in cell fate decision favoring apoptosis.

• Biomolecules & Therapeutics
• /
• 제13권2호
• /
• pp.113-117
• /
• 2005

Organosulfur compounds have been shown to exert an anti-cancer activity. In an attempt to develop novel chemopreventive and anti-cancer agents for liver cancer, we synthesized allylthiopyridazine derivatives. We have previously shown that allylthiopyridazine derivatives exert inhibitory effects on proliferation, invasion and migration of SK-Hep-1 hepatocarcinoma cells in vitro. The in vivo anti-tumor effect of 3-methvl-6-allylthiopy-ridazine, named as K6, was also reported. In this study, we further investigated the preclinical anti-cancer efficacy of K6 for hepatocarcinoma using nude mice xenografted with Hep-G2 hepatocellular carcinoma cells. K6(20-100 mg/kg, orally administered everyday for 30 days) markedly decreased the tumor volume of Hep-G2 cell-transplanted nude mice as evidenced by ultrasonographic and plethysmogranhic analyses. The inhibitory effect on tumor volume was lower than that exerted by doxorubicin (2 mg/kg), intravenously injected) which was used as a positive control. This study shows that K6 efficiently suppresses xenograft tumor growth, revealing K6 as apotential anti-cancer agent for suppressing in vivo progression of liver cancer. Given that hepatocarcinoma is among the most prevalent and lethal malignancies and there is no effective treatment to date, our study may contribute to the potential drug development for liver cancer.

• 대한한방종양학회지
• /
• 제7권1호
• /
• pp.61-75
• /
• 2001

The purpose of this study is to prove the angiogenesis effects of WhaYoungJiTongTang (hereinafter referred to as the 'WYJTT'). For the study, by utilizing liver cancer cell line; SK-HEP-1, lung cancer cell line: A549, stomach cancer cell line: AGS and bovine capillary endothelial cell: BCE, the effects of the WYJTT on toxicity and proliferation ability of cells and the effects on anti-angiogenesis of bovine capillary endothelial cell and of mice's aorta were studied. 1. Cell viability assay In comparison with the control group, when $100{\mu}g/ml$ of WYJTT was injected, the viability was reduced in SK-Hep-1, $400{\mu}g/ml$ in A549 and $200{\mu}g/ml$ in AGS. 2. Cell proliferation assay In comparison with the control group, when $600{\mu}g/ml$ of WYITT was injected, DNA synthesis was reduced to 35.1% in the SK-Hep-1, 56.0% in A549, and 25.8% in BCE (bovine capillary endothelial cell); and when $400{\mu}g/ml$ was injected, DNA synthesis was reduced to 12.1 in AGS. 3. Tube formation assay In the event that BCE is injected with WYJTT in each of its content gradient, the anti-angiogenesis was effective in amounts of $400{\mu}g/ml$ with 6 hours of the treatment. 4. Aortic ring assay In comparison with the control group, the angiogenesis was restricted to the remarkable degree in amount of $200{\mu}g/ml$: 10% in $400{\mu}g/ml$; and fully inhibited in each of $800{\mu}g/ml$ and $1600{\mu}g/ml$. As a result of the experiments mentioned above, WYJTT showed its anti-angiogenesis effects against cancer cell line.

• 한국축산식품학회지
• /
• 제22권4호
• /
• pp.348-351
• /
• 2002

유황오리 추출물의 각종 암세포에 대한 생육억제효과를 MTT assay를 이용하여 검토한 결과 10 mg/$m\ell$의 농도에서 KB(구강상피암) 89.5$\pm$0.7%, SNU-1(위암) 69.8$\pm$1.7%, K-562(백혈병) 79.8$\pm$2.8%, Farrow(흑색종) 82.7$\pm$2.6%, WiDr 결장암) 76.3$\pm$2.5%, SK-MES-1(폐암) 59.2 $\pm$4.4%, HL6O(백혈병) 60.5 +3.5%, Calu-3(폐암) 53.2$\pm$1.6%, HEP-2(후두암) 80.7$\pm$0.5%, P388(마우스 백혈병) 79.9$\pm$3.7%, 3LL(마우스 폐암) 87.2$\pm$3.3%의 효과가 있다는 사실이 판명되었다. 그리고 유황오리 추출물의 HP-20 column chromatography에서는 100% methyl alcohol 용출물이 HEP-2에 대한 생육억제효과가 있었으며, 10 mg/$m\ell$에서 99.1$\pm$0.4%의 생육억제효과가 나타났다. 또한 일반오리 추출물과 유황오리 추출물의 각종 암세포에 대한 생육억제효과를 비교하였을 때 거의 모든 암세포에서 유황오리 추출물의 효과가 더 높았다.

• Animal cells and systems
• /
• 제11권1호
• /
• pp.9-15
• /
• 2007

The naphthoquinone analog (2,3-dichloro-6,9-dihydroxy-1,4-naphtoquinone, NA) has an inhibitory effect on cdc25A protein phosphatase in vitro, which is responsible for G1/S transition during cell cycle. However, the exact mechanism inducing the growth inhibition is not understood. In this study, we investigated the regulatory mechanisms of growth arrest induced by NA, as a new potent inhibitor of cdc25A phosphatase, in human hepatocarcinoma SK-hep-1 cells. We found that NA induced the G1 arrest by perturbation of protein tyrosine dephosphorylation of Cdk2, which may be resulting from inhibition of cdc25A phosphatase. In addition, p21 was expressed in a p53-independent manner and participated in the NA-induced G1 arrest by inhibiting Cdk2 activity. Although the exact mechanism is not known, the p21 expression might be related to MAPK activation. From these results, we suggest that NA induces G1 arrest via inhibition of cdc25A and induction of p53-independent p21 expression in SK-Hep-1 cells.

• Journal of Applied Biological Chemistry
• /
• 제55권4호
• /
• pp.201-205
• /
• 2012

본 연구는 4종의 허브 식물의 부위별 MeOH 추출물에 대한 DPPH 라디칼 소거능, 세포독성 그리고 tyrosinase 저해활성을 검정하기 위해 시행되었다. yarrow의 지상부와 뿌리 추출물에서 총 폴리페놀 함량과 플라보노이드 함량이 가장 높았을 뿐만 아니라 yarrow의 지상부에서는 높은 DPPH 라디칼 소거능을 확인하였다. HeLa (uterus), SK-Hep-1 (liver), YD-15 (oral) 세포주를 이용하여 세포독성을 확인한 결과, feverfew의 지상부에서 가장 높은 세포독성을 보였으며 $102.58-138.68{\mu}g/mL$의 $IC_{50}$값을 확인하였다. 또한, mallow의 뿌리 추출물($71.24{\mu}g/mL$)에서는 대조구로 사용한 arbutin ($69.56{\mu}g/mL$)과 비교하여 tyrosinase 저해 활성에 효능이 있는 것으로 판단되었다.